Restriction map for pF1KB3095
 (Linear) (Six-base) MAPSORT of: KIBB3095.seq  Check: 9691  from: 1  to: 981



KIBB3095.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AatII G_ACGT'C

Cuts at:      0     939     981 
   Size:        939      42
 
AccI GT'mk_AC

Cuts at:      0     202     981 
   Size:        202     779
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     981 
   Size:        110     871
 
AflII C'TTAA_G

Cuts at:      0     357     981 
   Size:        357     624
 
AhdI GACnn_n'nnGTC

Cuts at:      0     940     981 
   Size:        940      41
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     948     980     981 
   Size:        948      32       1

  Fragments arranged by size: 

                 948      32       1
 
ApoI r'AATT_y

Cuts at:      0     176     697     763     927     981 
   Size:        176     521      66     164      54

  Fragments arranged by size: 

                 521     176     164      66      54
 
AvaI C'yCGr_G

Cuts at:      0     122     981 
   Size:        122     859
 
BanII G_rGCy'C

Cuts at:      0     738     981 
   Size:        738     243
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     451     981 
   Size:        451     530
 
BglII A'GATC_T

Cuts at:      0     128     500     981 
   Size:        128     372     481

  Fragments arranged by size: 

                 481     372     128
 
BmtI G_CTAG'C

Cuts at:      0     383     981 
   Size:        383     598
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     981 
   Size:         67     914
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     561     981 
   Size:        561     420
 
BsaHI Gr'CG_yC

Cuts at:      0     936     981 
   Size:        936      45
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     260     290     671     701     981 
   Size:        260      30     381      30     280

  Fragments arranged by size: 

                 381     280     260      30      30
 
BseYI C'CCAG_C

Cuts at:      0     641     981 
   Size:        641     340
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     981 
   Size:        100     881
 
BsiHKAI G_wGCw'C

Cuts at:      0     738     981 
   Size:        738     243
 
BsmI GAATG_Cn'

Cuts at:      0     418     981 
   Size:        418     563
 
Bsp1286I G_dGCh'C

Cuts at:      0     738     981 
   Size:        738     243
 
BspHI T'CATG_A

Cuts at:      0     711     981 
   Size:        711     270
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     542     981 
   Size:        542     439
 
BstEII G'GTnAC_C

Cuts at:      0     786     981 
   Size:        786     195
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     919     981 
   Size:        919      62
 
BstYI r'GATC_y

Cuts at:      0     128     500     981 
   Size:        128     372     481

  Fragments arranged by size: 

                 481     372     128
 
BstZ17I GTA'TAC

Cuts at:      0     203     981 
   Size:        203     778
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     956     981 
   Size:         19     937      25

  Fragments arranged by size: 

                 937      25      19
 
EcoICRI GAG'CTC

Cuts at:      0     736     981 
   Size:        736     245
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     110     981 
   Size:         67      43     871

  Fragments arranged by size: 

                 871      67      43
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     587     981 
   Size:        587     394
 
EcoRI G'AATT_C

Cuts at:      0     927     981 
   Size:        927      54
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     523     555     926     958     981 
   Size:        523      32     371      32      23

  Fragments arranged by size: 

                 523     371      32      32      23
 
MslI CAynn'nnrTG

Cuts at:      0     374     981 
   Size:        374     607
 
MspA1I CmG'CkG

Cuts at:      0     171     981 
   Size:        171     810
 
NheI G'CTAG_C

Cuts at:      0     379     981 
   Size:        379     602
 
PfoI T'CCnGG_A

Cuts at:      0      66     981 
   Size:         66     915
 
PsiI TTA'TAA

Cuts at:      0     326     981 
   Size:        326     655
 
SacI G_AGCT'C

Cuts at:      0     738     981 
   Size:        738     243
 
SfcI C'TryA_G

Cuts at:      0     476     981 
   Size:        476     505
 
SmlI C'TyrA_G

Cuts at:      0     357     981 
   Size:        357     624
 
SspI AAT'ATT

Cuts at:      0     432     981 
   Size:        432     549
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      62      94     260     292     981 
   Size:         62      32     166      32     689

  Fragments arranged by size: 

                 689     166      62      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     448     502     981 
   Size:        448      54     479

  Fragments arranged by size: 

                 479     448      54
 
ZraI GAC'GTC

Cuts at:      0     937     981 
   Size:        937      44
 

 Enzymes that do cut:

    AatII     AccI     AcuI    AflII     AhdI     AloI     ApoI     AvaI
    BanII     BbsI    BglII     BmtI     BpmI     BsaI    BsaHI    BsaXI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI   BstEII
    BstXI    BstYI  BstZ17I     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI
    Hin4I     MslI   MspA1I     NheI     PfoI     PsiI     SacI     SfcI
     SmlI     SspI     TstI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI   AflIII     AgeI     AleI     AlfI
    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaWI    BseRI    BsiEI    BsiWI    BsmBI    BspEI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
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