Restriction map for pF1KB0497
 (Circular) (Six-base) MAPSORT of: pF1KB0497.seq  Check: 4658  from: 1  to: 16394



pF1KB0497  16394 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2767    5498    6699    8694   11137   11838    2767 
   Size:       2731    1201    1995    2443     701    7323

  Fragments arranged by size: 

                7323    2731    2443    1995    1201     701
 
AccI GT'mk_AC

Cuts at:    524    2301    2420    4339    6782    7286    7514    8918   11117 
   Size:       1777     119    1919    2443     504     228    1404    2199

Cuts at:  11117   11401   12769   13432     524 
   Size:        284    1368     663    3486

  Fragments arranged by size: 

                3486    2443    2199    1919    1777    1404    1368     663
                 504     284     228     119
 
Acc65I G'GTAC_C

Cuts at:  13410   13410 
   Size:      16394
 
AclI AA'CG_TT

Cuts at:  13010   16234   13010 
   Size:       3224   13170
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    499    1153    3289    3370    3541    3751    4160    6140    7813 
   Size:        654    2136      81     171     210     409    1980    1673

Cuts at:   7813    8218    8263    8787    9634    9935    9949   10123   10369 
   Size:        405      45     524     847     301      14     174     246

Cuts at:  10369   10411   12977   14219   14651   15398   15666     499 
   Size:         42    2566    1242     432     747     268    1227

  Fragments arranged by size: 

                2566    2136    1980    1673    1242    1227     847     747
                 654     524     432     409     405     301     268     246
                 210     174     171      81      45      42      14
 
AfeI AGC'GCT

Cuts at:   7912   11695    7912 
   Size:       3783   12611
 
AflII C'TTAA_G

Cuts at:  12214   12214 
   Size:      16394
 
AflIII A'CryG_T

Cuts at:   2987    3644    7439    7580    7889    8748   11980   12249   13746 
   Size:        657    3795     141     309     859    3232     269    1497

Cuts at:  13746   14850   15827    2987 
   Size:       1104     977    3554

  Fragments arranged by size: 

                3795    3554    3232    1497    1104     977     859     657
                 309     269     141
 
AgeI A'CCGG_T

Cuts at:  12335   14782   12335 
   Size:       2447   13947
 
AhdI GACnn_n'nnGTC

Cuts at:   1919    5613    7723   13320    1919 
   Size:       3694    2110    5597    4993

  Fragments arranged by size: 

                5597    4993    3694    2110
 
AleI CACnn'nnGTG

Cuts at:  10861   12979   10861 
   Size:       2118   14276
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    233    4519    6715    9721   10690   11479   12470   13333     233 
   Size:       4286    2196    3006     969     789     991     863    3294

  Fragments arranged by size: 

                4286    3294    3006    2196     991     969     863     789
            
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    928     960    6628    6660     928 
   Size:         32    5668      32   10662

  Fragments arranged by size: 

               10662    5668      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:   1527    3309    3774    4314    4544    4768    5724    6006    7404 
   Size:       1782     465     540     230     224     956     282    1398

Cuts at:   7404    8418    8810    9105    9345   10005   10311   10347   10647 
   Size:       1014     392     295     240     660     306      36     300

Cuts at:  10647   10662   13699   15266    1527 
   Size:         15    3037    1567    2655

  Fragments arranged by size: 

                3037    2655    1782    1567    1398    1014     956     660
                 540     465     392     306     300     295     282     240
                 230     224      36      15
 
ApaI G_GGCC'C

Cuts at:    604    4838    7705    9411   12921     604 
   Size:       4234    2867    1706    3510    4077

  Fragments arranged by size: 

                4234    4077    3510    2867    1706
 
ApaLI G'TGCA_C

Cuts at:   1759    1849   15164    1759 
   Size:         90   13315    2989

  Fragments arranged by size: 

               13315    2989      90
 
ApoI r'AATT_y

Cuts at:    918    2434    2749    3538    3610    5255    8656   13398   15747 
   Size:       1516     315     789      72    1645    3401    4742    2349

Cuts at:  15747   16003     918 
   Size:        256    1309

  Fragments arranged by size: 

                4742    3401    2349    1645    1516    1309     789     315
                 256      72
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:      16394
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:      16394
 
AvaI C'yCGr_G

Cuts at:    185     322     353    1247    2235    4869    5264    5424    6703 
   Size:        137      31     894     988    2634     395     160    1279

Cuts at:   6703    6943    8369    8693    8887   10265   12554   13414     185 
   Size:        240    1426     324     194    1378    2289     860    3165

  Fragments arranged by size: 

                3165    2634    2289    1426    1378    1279     988     894
                 860     395     324     240     194     160     137      31
            
 
AvrII C'CTAG_G

Cuts at:   4479    6486    4479 
   Size:       2007   14387
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   6939    6972   12202   12235    6939 
   Size:         33    5230      33   11098

  Fragments arranged by size: 

               11098    5230      33      33
 
BamHI G'GATC_C

Cuts at:   6596    8039    8796    8813   11856   13419    6596 
   Size:       1443     757      17    3043    1563    9571

  Fragments arranged by size: 

                9571    3043    1563    1443     757      17
 
BanI G'GyrC_C

Cuts at:   1053    1348    3242    3426    5895    6933    7275    7807    8631 
   Size:        295    1894     184    2469    1038     342     532     824

Cuts at:   8631    8706   11390   12272   13410   13824   14072   14107    1053 
   Size:         75    2684     882    1138     414     248      35    3340

  Fragments arranged by size: 

                3340    2684    2469    1894    1138    1038     882     824
                 532     414     342     295     248     184      75      35
            
 
BanII G_rGCy'C

Cuts at:    257     323     604    1724    2373    3788    4838    5506    6863 
   Size:         66     281    1120     649    1415    1050     668    1357

Cuts at:   6863    7147    7705    8207    8543    9411    9521    9752   12921 
   Size:        284     558     502     336     868     110     231    3169

Cuts at:  12921   13166   13408   14438     257 
   Size:        245     242    1030    2213

  Fragments arranged by size: 

                3169    2213    1415    1357    1120    1050    1030     868
                 668     649     558     502     336     284     281     245
                 242     231     110      66
 
BbeI G_GCGC'C

Cuts at:   1057    3246   12276   13828   14076    1057 
   Size:       2189    9030    1552     248    3375

  Fragments arranged by size: 

                9030    3375    2189    1552     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1478    2526    2817    3477    3856    4493    7378    8052    8225 
   Size:       1048     291     660     379     637    2885     674     173

Cuts at:   8225    8251   12983    1478 
   Size:         26    4732    4889

  Fragments arranged by size: 

                4889    4732    2885    1048     674     660     637     379
                 291     173      26
 
BbvCI CC'TCA_GC

Cuts at:   1536    6514    6851    8617    8782    9812   10602    1536 
   Size:       4978     337    1766     165    1030     790    7328

  Fragments arranged by size: 

                7328    4978    1766    1030     790     337     165
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   3385    3419    5673    5707    6244    6278   11362   11396    3385 
   Size:         34    2254      34     537      34    5084      34    8383

  Fragments arranged by size: 

                8383    5084    2254     537      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   1450    1944    3634    6774   10405   13085   14292   15053   16394 
   Size:        494    1690    3140    3631    2680    1207     761    1341

Cuts at:  16394    1450 
   Size:       1450

  Fragments arranged by size: 

                3631    3140    2680    1690    1450    1341    1207     761
                 494
 
BclI T'GATC_A

Cuts at:   1199    1529    4089    5060    7541    7814    9575   13341   13914 
   Size:        330    2560     971    2481     273    1761    3766     573

Cuts at:  13914    1199 
   Size:       3679

  Fragments arranged by size: 

                3766    3679    2560    2481    1761     971     573     330
                 273
 
BfrBI ATG'CAT

Cuts at:    962    7938     962 
   Size:       6976    9418
 
BglI GCCn_nnn'nGGC

Cuts at:    187    5522    6754    8428    9406   10945   12916     187 
   Size:       5335    1232    1674     978    1539    1971    3665

  Fragments arranged by size: 

                5335    3665    1971    1674    1539    1232     978
 
BglII A'GATC_T

Cuts at:    653    2999    4166    6192    8960    9366   10127   13909   15946 
   Size:       2346    1167    2026    2768     406     761    3782    2037

Cuts at:  15946     653 
   Size:       1101

  Fragments arranged by size: 

                3782    2768    2346    2037    2026    1167    1101     761
                 406
 
BlpI GC'TnA_GC

Cuts at:    251    2093    5326    9782   11884   12169   13506     251 
   Size:       1842    3233    4456    2102     285    1337    3139

  Fragments arranged by size: 

                4456    3233    3139    2102    1842    1337     285
 
Bme1580I G_kGCm'C

Cuts at:    604     761     894    1353    1763    1853    4838    6045    6938 
   Size:        157     133     459     410      90    2985    1207     893

Cuts at:   6938    7280    7705    8300    8636    9411   10284   11658   12921 
   Size:        342     425     595     336     775     873    1374    1263

Cuts at:  12921   13363   14019   14112   15168     604 
   Size:        442     656      93    1056    1830

  Fragments arranged by size: 

                2985    1830    1374    1263    1207    1056     893     873
                 775     656     595     459     442     425     410     342
                 336     157     133      93      90
 
BmgBI CAC'GTC

Cuts at:   1560    8552   11983    1560 
   Size:       6992    3431    5971

  Fragments arranged by size: 

                6992    5971    3431
 
BmrI ACTGGGnnnn_n'

Cuts at:   1728    2055    2265    3328    3834    3963    4062    4401    5355 
   Size:        327     210    1063     506     129      99     339     954

Cuts at:   5355    7376   11319   11784   12273   13606   13687   14022   16166 
   Size:       2021    3943     465     489    1333      81     335    2144

Cuts at:  16166    1728 
   Size:       1956

  Fragments arranged by size: 

                3943    2144    2021    1956    1333    1063     954     506
                 489     465     339     335     327     210     129      99
                  81
 
BmtI G_CTAG'C

Cuts at:  12643   12643 
   Size:      16394
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:  14761   14761 
   Size:      16394
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    772     952    1402    1738    3271    3331    3469    3781    3796 
   Size:        180     450     336    1533      60     138     312      15

Cuts at:   3796    4756    5672    6853    7395    7624    9211    9433    9871 
   Size:        960     916    1181     542     229    1587     222     438

Cuts at:   9871    9955   10027   10669   10717   10780   10897   11092   11611 
   Size:         84      72     642      48      63     117     195     519

Cuts at:  11611   12370   12563     772 
   Size:        759     193    4603

  Fragments arranged by size: 

                4603    1587    1533    1181     960     916     759     642
                 542     519     450     438     336     312     229     222
                 195     193     180     138     117      84      72      63
                  60      48      15
 
Bpu10I CC'TnA_GC

Cuts at:    272    1193    1536    3223    6514    6851    7574    8617    8622 
   Size:        921     343    1687    3291     337     723    1043       5

Cuts at:   8622    8782    9812   10602   10691     272 
   Size:        160    1030     790      89    5975

  Fragments arranged by size: 

                5975    3291    1687    1043    1030     921     790     723
                 343     337     160      89       5
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    278    2227    2959    3151    5128    8527   10735   12592   13570 
   Size:       1949     732     192    1977    3399    2208    1857     978

Cuts at:  13570   14449   14941   15239     278 
   Size:        879     492     298    1433

  Fragments arranged by size: 

                3399    2208    1977    1949    1857    1433     978     879
                 732     492     298     192
 
BsaI GGTCTCn'nnnn_

Cuts at:    838    3883    4675    5533    6238    6425   10591   10873   10882 
   Size:       3045     792     858     705     187    4166     282       9

Cuts at:  10882   12760   16098     838 
   Size:       1878    3338    1134

  Fragments arranged by size: 

                4166    3338    3045    1878    1134     858     792     705
                 282     187       9
 
BsaAI yAC'GTr

Cuts at:   1728    2079    7518   11239   13747    1728 
   Size:        351    5439    3721    2508    4375

  Fragments arranged by size: 

                5439    4375    3721    2508     351
 
BsaBI GATnn'nnATC

Cuts at:      6   11476   11861   13908   15945       6 
   Size:      11470     385    2047    2037     455

  Fragments arranged by size: 

               11470    2047    2037     455     385
 
BsaHI Gr'CG_yC

Cuts at:   1054    1773    2068    3243    8409   10232   12273   13825   14073 
   Size:        719     295    1175    5166    1823    2041    1552     248

Cuts at:  14073   14775   16271    1054 
   Size:        702    1496    1177

  Fragments arranged by size: 

                5166    2041    1823    1552    1496    1177    1175     719
                 702     295     248
 
BsaWI w'CCGG_w

Cuts at:   1741    4589    4982   12335   13133   13583   14104   14782   15056 
   Size:       2848     393    7353     798     450     521     678     274

Cuts at:  15056   15203   15614    1741 
   Size:        147     411    2521

  Fragments arranged by size: 

                7353    2848    2521     798     678     521     450     411
                 393     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    928     958    3004    3034    6628    6658   10222   10252   11530 
   Size:         30    2046      30    3594      30    3564      30    1278

Cuts at:  11530   11560   13012   13042   13279   13309     928 
   Size:         30    1452      30     237      30    4013

  Fragments arranged by size: 

                4013    3594    3564    2046    1452    1278     237      30
                  30      30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    280     949    3133    3844    7114    9250    9910   10003   10024 
   Size:        669    2184     711    3270    2136     660      93      21

Cuts at:  10024   10714   11737   12208     280 
   Size:        690    1023     471    4466

  Fragments arranged by size: 

                4466    3270    2184    2136    1023     711     690     669
                 660     471      93      21
 
BseYI C'CCAG_C

Cuts at:    489    1307    1754    3238    4837    7014    7592    8164    8530 
   Size:        818     447    1484    1599    2177     578     572     366

Cuts at:   8530    8826    9084    9465    9638    9943   10655   10662   10940 
   Size:        296     258     381     173     305     712       7     278

Cuts at:  10940   11690   13127   13819   14577   15154   15650     489 
   Size:        750    1437     692     758     577     496    1233

  Fragments arranged by size: 

                2177    1599    1484    1437    1233     818     758     750
                 712     692     578     577     572     496     447     381
                 366     305     296     278     258     173       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    601     694    1012    1396    2236    2338    2476    3130    3202 
   Size:         93     318     384     840     102     138     654      72

Cuts at:   3202    3241    4465    4792    5476    6019    6345    6590    7489 
   Size:         39    1224     327     684     543     326     245     899

Cuts at:   7489    8749    8773    8826    9277   10456   10837     601 
   Size:       1260      24      53     451    1179     381    6158

  Fragments arranged by size: 

                6158    1260    1224    1179     899     840     684     654
                 543     451     384     381     327     326     318     245
                 138     102      93      72      53      39      24
 
BsiEI CG_ry'CG

Cuts at:    104    9669   13936   13982   15190     104 
   Size:       9565    4267      46    1208    1308

  Fragments arranged by size: 

                9565    4267    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    257    1763    1812    1853    3788    4597    7430    8315    8543 
   Size:       1506      49      41    1935     809    2833     885     228

Cuts at:   8543    8777   10970   11678   13408   14186   14376   15168     257 
   Size:        234    2193     708    1730     778     190     792    1483

  Fragments arranged by size: 

                2833    2193    1935    1730    1506    1483     885     809
                 792     778     708     234     228     190      49      41
            
 
BsiWI C'GTAC_G

Cuts at:   7648    7648 
   Size:      16394
 
BsmI GAATG_Cn'

Cuts at:    222    1937    2114    6392     222 
   Size:       1715     177    4278   10224

  Fragments arranged by size: 

               10224    4278    1715     177
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2059    9678    2059 
   Size:       7619    8775
 
Bsp1286I G_dGCh'C

Cuts at:    257     323     604     761     894    1353    1724    1763    1812 
   Size:         66     281     157     133     459     371      39      49

Cuts at:   1812    1853    2373    3788    4597    4838    5506    6045    6863 
   Size:         41     520    1415     809     241     668     539     818

Cuts at:   6863    6938    7147    7280    7430    7705    8207    8300    8315 
   Size:         75     209     133     150     275     502      93      15

Cuts at:   8315    8543    8636    8777    9411    9521    9752   10284   10970 
   Size:        228      93     141     634     110     231     532     686

Cuts at:  10970   11658   11678   12921   13166   13363   13408   14019   14112 
   Size:        688      20    1243     245     197      45     611      93

Cuts at:  14112   14186   14376   14438   15168     257 
   Size:         74     190      62     730    1483

  Fragments arranged by size: 

                1483    1415    1243     818     809     730     688     686
                 668     634     611     539     532     520     502     459
                 371     281     275     245     241     231     228     209
                 197     190     157     150     141     133     133     110
                  93      93      93      75      74      66      62      49
                  45      41      39      20      15
 
BspEI T'CCGG_A

Cuts at:   1741    4589    4982   15614    1741 
   Size:       2848     393   10632    2521

  Fragments arranged by size: 

               10632    2848    2521     393
 
BspHI T'CATG_A

Cuts at:   1160    1790    2513    5150   11357   13373   15570    1160 
   Size:        630     723    2637    6207    2016    2197    1984

  Fragments arranged by size: 

                6207    2637    2197    2016    1984     723     630
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1804    2117    2498    2767    4031    4739    5498    6699    8694 
   Size:        313     381     269    1264     708     759    1201    1995

Cuts at:   8694    8771   10370   10388   11137   11838   11916   12039   13325 
   Size:         77    1599      18     749     701      78     123    1286

Cuts at:  13325   13444   13658   13960   14341    1804 
   Size:        119     214     302     381    3857

  Fragments arranged by size: 

                3857    1995    1599    1286    1264    1201     759     749
                 708     701     381     381     313     302     269     214
                 123     119      78      77      18
 
BsrBI CCG'CTC

Cuts at:     50    2052    5124   10686   12822   14686   14740   16224   16389 
   Size:       2002    3072    5562    2136    1864      54    1484     165

Cuts at:  16389      50 
   Size:         55

  Fragments arranged by size: 

                5562    3072    2136    2002    1864    1484     165      55
                  54
 
BsrDI GCAATG_nn'

Cuts at:   1957    3077    3198    4217    5482    5912    6749   11463   12224 
   Size:       1120     121    1019    1265     430     837    4714     761

Cuts at:  12224   14306    1957 
   Size:       2082    4045

  Fragments arranged by size: 

                4714    4045    2082    1265    1120    1019     837     761
                 430     121
 
BsrFI r'CCGG_y

Cuts at:   3857    5429    5749    8069   10795   12229   12269   12335   14392 
   Size:       1572     320    2320    2726    1434      40      66    2057

Cuts at:  14392   14573   14782    3857 
   Size:        181     209    5469

  Fragments arranged by size: 

                5469    2726    2320    2057    1572    1434     320     209
                 181      66      40
 
BsrGI T'GTAC_A

Cuts at:    501    2469    2576    5975    7892     501 
   Size:       1968     107    3399    1917    9003

  Fragments arranged by size: 

                9003    3399    1968    1917     107
 
BssHII G'CGCG_C

Cuts at:   1750    1750 
   Size:      16394
 
BssSI C'ACGA_G

Cuts at:   3337    3394    3818    9952   11381   11617   14665   15023    3337 
   Size:         57     424    6134    1429     236    3048     358    4708

  Fragments arranged by size: 

                6134    4708    3048    1429     424     358     236      57
            
 
BstAPI GCAn_nnn'nTGC

Cuts at:    959    1419    1572    2571    7404    8310    8772    9105   11472 
   Size:        460     153     999    4833     906     462     333    2367

Cuts at:  11472   13771     959 
   Size:       2299    3582

  Fragments arranged by size: 

                4833    3582    2367    2299     999     906     462     460
                 333     153
 
BstBI TT'CG_AA

Cuts at:   1893    3608    6878   11144   14755    1893 
   Size:       1715    3270    4266    3611    3532

  Fragments arranged by size: 

                4266    3611    3532    3270    1715
 
BstEII G'GTnAC_C

Cuts at:   2505    4962    8469    2505 
   Size:       2457    3507   10430

  Fragments arranged by size: 

               10430    3507    2457
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3636    3687    4828    5773    6354    7347    9054   11190   11441 
   Size:         51    1141     945     581     993    1707    2136     251

Cuts at:  11441   12888    3636 
   Size:       1447    7142

  Fragments arranged by size: 

                7142    2136    1707    1447    1141     993     945     581
                 251      51
 
BstYI r'GATC_y

Cuts at:      1     653    1079    1647    2228    2915    2999    3380    4073 
   Size:        652     426     568     581     687      84     381     693

Cuts at:   4073    4166    5075    5355    5720    5807    6093    6192    6596 
   Size:         93     909     280     365      87     286      99     404

Cuts at:   6596    8039    8229    8666    8796    8813    8960    9366   10127 
   Size:       1443     190     437     130      17     147     406     761

Cuts at:  10127   10769   11856   12599   12683   13419   13886   13909   14244 
   Size:        642    1087     743      84     736     467      23     335

Cuts at:  14244   14490   15502   15588   15600   15946       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1443    1087    1012     909     761     743     736     693
                 687     652     642     581     568     467     449     437
                 426     406     404     381     365     346     335     286
                 280     246     190     147     130      99      93      87
                  86      84      84      23      17      12
 
BstZ17I GTA'TAC

Cuts at:   2302    2421   11402    2302 
   Size:        119    8981    7294

  Fragments arranged by size: 

                8981    7294     119
 
Bsu36I CC'TnA_GG

Cuts at:   5002   10298   12431    5002 
   Size:       5296    2133    8965

  Fragments arranged by size: 

                8965    5296    2133
 
BtgI C'CryG_G

Cuts at:    277    3884    3893    3900    5477    5618    5888    6614    7040 
   Size:       3607       9       7    1577     141     270     726     426

Cuts at:   7040    7358    7697    7901    8789    9713   11349     277 
   Size:        318     339     204     888     924    1636    5322

  Fragments arranged by size: 

                5322    3607    1636    1577     924     888     726     426
                 339     318     270     204     141       9       7
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3016    4955    6126    6678    6712    7365   10713   11440   11718 
   Size:       1939    1171     552      34     653    3348     727     278

Cuts at:  11718   14344   14525   14688    3016 
   Size:       2626     181     163    4722

  Fragments arranged by size: 

                4722    3348    2626    1939    1171     727     653     552
                 278     181     163      34
 
BtsI GCAGTG_nn'

Cuts at:    389    2572    2635    3186    5063    9235    9379   12974   13361 
   Size:       2183      63     551    1877    4172     144    3595     387

Cuts at:  13361   13637     389 
   Size:        276    3146

  Fragments arranged by size: 

                4172    3595    3146    2183    1877     551     387     276
                 144      63
 
ClaI AT'CG_AT

Cuts at:  10169   10169 
   Size:      16394
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   5895    5930   12225   12260    5895 
   Size:         35    6295      35   10029

  Fragments arranged by size: 

               10029    6295      35      35
 
DraI TTT'AAA

Cuts at:  13393   13393 
   Size:      16394
 
DraIII CAC_nnn'GTG

Cuts at:    304    1572    2294    5102    6626    8772   11778   12078     304 
   Size:       1268     722    2808    1524    2146    3006     300    4620

  Fragments arranged by size: 

                4620    3006    2808    2146    1524    1268     722     300
            
 
DrdI GACnn_nn'nnGTC

Cuts at:   1122    8414   10717   14100   14958    1122 
   Size:       7292    2303    3383     858    2558

  Fragments arranged by size: 

                7292    3383    2558    2303     858
 
EaeI y'GGCC_r

Cuts at:    144     197    1708    3362    3689    3855    5049    5838    5963 
   Size:         53    1511    1654     327     166    1194     789     125

Cuts at:   5963    6755    6989    7325    9666   12441   12578   12878   13979 
   Size:        792     234     336    2341    2775     137     300    1101

Cuts at:  13979   14153   14544   14571   15916     144 
   Size:        174     391      27    1345     622

  Fragments arranged by size: 

                2775    2341    1654    1511    1345    1194    1101     792
                 789     622     391     336     327     300     234     174
                 166     137     125      53      27
 
EagI C'GGCC_G

Cuts at:   9666   13979    9666 
   Size:       4313   12081
 
EarI CTCTTCn'nnn_

Cuts at:    349    1273    2435    2698    3066    3695    6101    6832    7105 
   Size:        924    1162     263     368     629    2406     731     273

Cuts at:   7105    7838    7951    8320    8657    8734    9368    9389    9911 
   Size:        733     113     369     337      77     634      21     522

Cuts at:   9911   12554   12776   13219   14417   14627     349 
   Size:       2643     222     443    1198     210    2116

  Fragments arranged by size: 

                2643    2406    2116    1198    1162     924     733     731
                 634     629     522     443     369     368     337     273
                 263     222     210     113      77      21
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    391    5365    5866    7603    8806   11863   14602   14912   15058 
   Size:       4974     501    1737    1203    3057    2739     310     146

Cuts at:  15058   16204     391 
   Size:       1146     581

  Fragments arranged by size: 

                4974    3057    2739    1737    1203    1146     581     501
                 310     146
 
EcoICRI GAG'CTC

Cuts at:    255    3786    8541   13406     255 
   Size:       3531    4755    4865    3243

  Fragments arranged by size: 

                4865    4755    3531    3243
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    499     772     952    1153    1402    1738    3271    3289    3331 
   Size:        273     180     201     249     336    1533      18      42

Cuts at:   3331    3370    3469    3541    3751    3781    3796    4160    4756 
   Size:         39      99      72     210      30      15     364     596

Cuts at:   4756    5672    6140    6853    7395    7624    7813    8218    8263 
   Size:        916     468     713     542     229     189     405      45

Cuts at:   8263    8787    9211    9433    9634    9871    9935    9949    9955 
   Size:        524     424     222     201     237      64      14       6

Cuts at:   9955   10027   10123   10369   10411   10669   10717   10780   10897 
   Size:         72      96     246      42     258      48      63     117

Cuts at:  10897   11092   11611   12370   12563   12977   14219   14651   15398 
   Size:        195     519     759     193     414    1242     432     747

Cuts at:  15398   15666     499 
   Size:        268    1227

  Fragments arranged by size: 

                1533    1242    1227     916     759     747     713     596
                 542     524     519     468     432     424     414     405
                 364     336     273     268     258     249     246     237
                 229     222     210     201     201     195     193     189
                 180     117      99      96      72      72      64      63
                  48      45      42      42      39      30      18      15
                  14       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1593    2494    3778    5557    6301    8887    9394   10009   11494 
   Size:        901    1284    1779     744    2586     507     615    1485

Cuts at:  11494   11716   11752   12676    1593 
   Size:        222      36     924    5311

  Fragments arranged by size: 

                5311    2586    1779    1485    1284     924     901     744
                 615     507     222      36
 
EcoO109I rG'GnC_Cy

Cuts at:    289     429     600     601     925    1327    1503    2106    2190 
   Size:        140     171       1     324     402     176     603      84

Cuts at:   2190    2427    3762    4834    4918    4974    5515    5550    5562 
   Size:        237    1335    1072      84      56     541      35      12

Cuts at:   5562    5601    6735    7354    7589    7702    8880    9408    9626 
   Size:         39    1134     619     235     113    1178     528     218

Cuts at:   9626   10002   10014   10072   10308   10344   10644   11727   11961 
   Size:        376      12      58     236      36     300    1083     234

Cuts at:  11961   12567   13104   13533     289 
   Size:        606     537     429    3150

  Fragments arranged by size: 

                3150    1335    1178    1134    1083    1072     619     606
                 603     541     537     528     429     402     376     324
                 300     237     236     235     234     218     176     171
                 140     113      84      84      58      56      39      36
                  35      12      12       1
 
EcoRI G'AATT_C

Cuts at:   2749    3610   13398    2749 
   Size:        861    9788    5745

  Fragments arranged by size: 

                9788    5745     861
 
EcoRV GAT'ATC

Cuts at:   8508    9660    8508 
   Size:       1152   15242
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    360    3039    3375   14413     360 
   Size:       2679     336   11038    2341

  Fragments arranged by size: 

               11038    2679    2341     336
 
FspI TGC'GCA

Cuts at:   2113   10513   14175    2113 
   Size:       8400    3662    4332

  Fragments arranged by size: 

                8400    4332    3662
 
FspAI rTGC'GCAy

Cuts at:  10513   10513 
   Size:      16394
 
HaeII r_GCGC'y

Cuts at:   1057    3045    3246    6081    7242    7914   11697   12276   13232 
   Size:       1988     201    2835    1161     672    3783     579     956

Cuts at:  13232   13828   14076   15098   16077    1057 
   Size:        596     248    1022     979    1374

  Fragments arranged by size: 

                3783    2835    1988    1374    1161    1022     979     956
                 672     596     579     248     201
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    723     755    1900    1905    1932    1937    3993    4025    4081 
   Size:         32    1145       5      27       5    2056      32      56

Cuts at:   4081    4113    4475    4507    4584    4616    5599    5631    5686 
   Size:         32     362      32      77      32     983      32      55

Cuts at:   5686    5718    6697    6729    7709    7741    8002    8034    8492 
   Size:         32     979      32     980      32     261      32     458

Cuts at:   8492    8524    8656    8688    9433    9465    9583    9615   10222 
   Size:         32     132      32     745      32     118      32     607

Cuts at:  10222   10254   10281   10313   11600   11632   11805   11837   12162 
   Size:         32      27      32    1287      32     173      32     325

Cuts at:  12162   12194   12451   12483   13012   13044   13279   13306   13311 
   Size:         32     257      32     529      32     235      27       5

Cuts at:  13311   13326   13338   13358   14236   14266   14268   14298     723 
   Size:         15      12      20     878      30       2      30    2819

  Fragments arranged by size: 

                2819    2056    1287    1145     983     980     979     878
                 745     607     529     458     362     325     261     257
                 235     173     132     118      77      56      55      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      30      30      27      27
                  27      20      15      12       5       5       5       2
            
 
HincII GTy'rAC

Cuts at:   1227    2001    4536    5589    6671    7287    7437   10107   10623 
   Size:        774    2535    1053    1082     616     150    2670     516

Cuts at:  10623   13433    1227 
   Size:       2810    4188

  Fragments arranged by size: 

                4188    2810    2670    2535    1082    1053     774     616
                 516     150
 
HindIII A'AGCT_T

Cuts at:   4104    5281    7825    4104 
   Size:       1177    2544   12673

  Fragments arranged by size: 

               12673    2544    1177
 
KasI G'GCGC_C

Cuts at:   1053    3242   12272   13824   14072    1053 
   Size:       2189    9030    1552     248    3375

  Fragments arranged by size: 

                9030    3375    2189    1552     248
 
KpnI G_GTAC'C

Cuts at:  13414   13414 
   Size:      16394
 
MfeI C'AATT_G

Cuts at:   5882   12792    5882 
   Size:       6910    9484
 
MluI A'CGCG_T

Cuts at:   8748   15827    8748 
   Size:       7079    9315
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    263    1928    3513    4809    5255    7183    8993    9164   10601 
   Size:       1665    1585    1296     446    1928    1810     171    1437

Cuts at:  10601   12167   15065   15249     263 
   Size:       1566    2898     184    1408

  Fragments arranged by size: 

                2898    1928    1810    1665    1585    1566    1437    1408
                1296     446     184     171
 
MscI TGG'CCA

Cuts at:    199    1710    3364    3691    5051    5965    6991    7327   12443 
   Size:       1511    1654     327    1360     914    1026     336    5116

Cuts at:  12443   12880   14155     199 
   Size:        437    1275    2438

  Fragments arranged by size: 

                5116    2438    1654    1511    1360    1275    1026     914
                 437     336     327
 
MslI CAynn'nnrTG

Cuts at:    793    2498    3191    3685    4960    5695    5771    5818    5893 
   Size:       1705     693     494    1275     735      76      47      75

Cuts at:   5893    6352    6697    7549    7933    8563    9032    9253    9703 
   Size:        459     345     852     384     630     469     221     450

Cuts at:   9703   10672   10861   11177   11683   11938   12452   12703   12763 
   Size:        969     189     316     506     255     514     251      60

Cuts at:  12763   12886   12979   14383   14510     793 
   Size:        123      93    1404     127    2677

  Fragments arranged by size: 

                2677    1705    1404    1275     969     852     735     693
                 630     514     506     494     469     459     450     384
                 345     316     255     251     221     189     127     123
                  93      76      75      60      47
 
MspA1I CmG'CkG

Cuts at:    990    1307    2467    2936    4080    4503    6006    6371    6770 
   Size:        317    1160     469    1144     423    1503     365     399

Cuts at:   6770    6953    7018    8405   10051   10659   10821   10875   10944 
   Size:        183      65    1387    1646     608     162      54      69

Cuts at:  10944   11667   11844   12020   12583   13505   13819   14179   15192 
   Size:        723     177     176     563     922     314     360    1013

Cuts at:  15192   15437     990 
   Size:        245    1947

  Fragments arranged by size: 

                1947    1646    1503    1387    1160    1144    1013     922
                 723     608     563     469     423     399     365     360
                 317     314     245     183     177     176     162      69
                  65      54
 
NaeI GCC'GGC

Cuts at:   5431    5751   12231   14575    5431 
   Size:        320    6480    2344    7250

  Fragments arranged by size: 

                7250    6480    2344     320
 
NarI GG'CG_CC

Cuts at:   1054    3243   12273   13825   14073    1054 
   Size:       2189    9030    1552     248    3375

  Fragments arranged by size: 

                9030    3375    2189    1552     248
 
NcoI C'CATG_G

Cuts at:   3884    5618    5888    7040    7697    8789    9713    3884 
   Size:       1734     270    1152     657    1092     924   10565

  Fragments arranged by size: 

               10565    1734    1152    1092     924     657     270
 
NdeI CA'TA_TG

Cuts at:  11174   11174 
   Size:      16394
 
NgoMIV G'CCGG_C

Cuts at:   5429    5749   12229   14573    5429 
   Size:        320    6480    2344    7250

  Fragments arranged by size: 

                7250    6480    2344     320
 
NheI G'CTAG_C

Cuts at:  12639   12639 
   Size:      16394
 
NruI TCG'CGA

Cuts at:   3214    6697    3214 
   Size:       3483   12911
 
NsiI A_TGCA'T

Cuts at:    964    7940     964 
   Size:       6976    9418
 
NspI r_CATG'y

Cuts at:    498    1659    1992    2308    2991    3648    4150    6569    6876 
   Size:       1161     333     316     683     657     502    2419     307

Cuts at:   6876    7443    7584    7893    7986    8382    9702   12253   13242 
   Size:        567     141     309      93     396    1320    2551     989

Cuts at:  13242   13447   13947   14854     498 
   Size:        205     500     907    2038

  Fragments arranged by size: 

                2551    2419    2038    1320    1161     989     907     683
                 657     567     502     500     396     333     316     309
                 307     205     141      93
 
PasI CC'CwG_GG

Cuts at:   1353    5565    7708   11712    1353 
   Size:       4212    2143    4004    6035

  Fragments arranged by size: 

                6035    4212    4004    2143
 
PciI A'CATG_T

Cuts at:   2987    3644    7439    7580    7889   12249   14850    2987 
   Size:        657    3795     141     309    4360    2601    4531

  Fragments arranged by size: 

                4531    4360    3795    2601     657     309     141
 
PflMI CCAn_nnn'nTGG

Cuts at:    557    1152    2079    2363    2502    4229    4314    6527    7003 
   Size:        595     927     284     139    1727      85    2213     476

Cuts at:   7003    7418    8418    8855   10005   10662   12876     557 
   Size:        415    1000     437    1150     657    2214    4075

  Fragments arranged by size: 

                4075    2214    2213    1727    1150    1000     927     657
                 595     476     437     415     284     139      85
 
PfoI T'CCnGG_A

Cuts at:   1082    1442    2216    3248    3446    4064    4613    4643   10199 
   Size:        360     774    1032     198     618     549      30    5556

Cuts at:  10199    1082 
   Size:       7277

  Fragments arranged by size: 

                7277    5556    1032     774     618     549     360     198
                  30
 
PmeI GTTT'AAAC

Cuts at:  13393   13393 
   Size:      16394
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    928     960    4388    4420    6628    6660   15533   15565     928 
   Size:         32    3428      32    2208      32    8873      32    1757

  Fragments arranged by size: 

                8873    3428    2208    1757      32      32      32      32
            
 
PpuMI rG'GwC_Cy

Cuts at:    289     429     925    1327    1503    2106    2427    3762    4974 
   Size:        140     496     402     176     603     321    1335    1212

Cuts at:   4974    5550    6735    7354    7589   10014   10072   12567     289 
   Size:        576    1185     619     235    2425      58    2495    4116

  Fragments arranged by size: 

                4116    2495    2425    1335    1212    1185     619     603
                 576     496     402     321     235     176     140      58
            
 
PspOMI G'GGCC_C

Cuts at:    600    4834    7701    9407   12917     600 
   Size:       4234    2867    1706    3510    4077

  Fragments arranged by size: 

                4234    4077    3510    2867    1706
 
PspXI vC'TCGA_Gb

Cuts at:   8887    8887 
   Size:      16394
 
PstI C_TGCA'G

Cuts at:    248     783    2768    2966    3875    5523    7400   10367   11666 
   Size:        535    1985     198     909    1648    1877    2967    1299

Cuts at:  11666   11849   12107   12680   12989   13212   13441   14126     248 
   Size:        183     258     573     309     223     229     685    2516

  Fragments arranged by size: 

                2967    2516    1985    1877    1648    1299     909     685
                 573     535     309     258     229     223     198     183
            
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:      16394
 
PvuII CAG'CTG

Cuts at:    990    2467    4503    6006    7018   10051   10659   10821   10875 
   Size:       1477    2036    1503    1012    3033     608     162      54

Cuts at:  10875   11667   11844   13819   14179     990 
   Size:        792     177    1975     360    3205

  Fragments arranged by size: 

                3205    3033    2036    1975    1503    1477    1012     792
                 608     360     177     162      54
 
RsrII CG'GwC_CG

Cuts at:  14589   14589 
   Size:      16394
 
SacI G_AGCT'C

Cuts at:    257    3788    8543   13408     257 
   Size:       3531    4755    4865    3243

  Fragments arranged by size: 

                4865    4755    3531    3243
 
SalI G'TCGA_C

Cuts at:   7285   13431    7285 
   Size:       6146   10248
 
SanDI GG'GwC_CC

Cuts at:   1327    7354    7589   10072    1327 
   Size:       6027     235    2483    7649

  Fragments arranged by size: 

                7649    6027    2483     235
 
SapI GCTCTTCn'nnn_

Cuts at:   1273    8320   13219   14417   14627    1273 
   Size:       7047    4899    1198     210    3040

  Fragments arranged by size: 

                7047    4899    3040    1198     210
 
SbfI CC_TGCA'GG

Cuts at:   5523   13441    5523 
   Size:       7918    8476
 
ScaI AGT'ACT

Cuts at:   5413   15911    5413 
   Size:      10498    5896
 
SexAI A'CCwGG_T

Cuts at:   4058    4325    4529    5162    7307    9242   10025    4058 
   Size:        267     204     633    2145    1935     783   10427

  Fragments arranged by size: 

               10427    2145    1935     783     633     267     204
 
SfcI C'TryA_G

Cuts at:     33     244     434     779    2764    2962    3871    4196    5519 
   Size:        211     190     345    1985     198     909     325    1323

Cuts at:   5519    6085    6397    7396   10363   10481   10587   11118   11662 
   Size:        566     312     999    2967     118     106     531     544

Cuts at:  11662   11845   12103   12676   12985   13208   13437   14122   15115 
   Size:        183     258     573     309     223     229     685     993

Cuts at:  15115   15306      33 
   Size:        191    1121

  Fragments arranged by size: 

                2967    1985    1323    1121     999     993     909     685
                 573     566     544     531     345     325     312     309
                 258     229     223     211     198     191     190     183
                 118     106
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   6754    9406   12916    6754 
   Size:       2652    3510   10232

  Fragments arranged by size: 

               10232    3510    2652
 
SfoI GGC'GCC

Cuts at:   1055    3244   12274   13826   14074    1055 
   Size:       2189    9030    1552     248    3375

  Fragments arranged by size: 

                9030    3375    2189    1552     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:      16394
 
SmaI CCC'GGG

Cuts at:   2237    5266    8695   12556   13416    2237 
   Size:       3029    3429    3861     860    5215

  Fragments arranged by size: 

                5215    3861    3429    3029     860
 
SmlI C'TyrA_G

Cuts at:    293    2242    2974    3166    5143    8542    8887   10750   12214 
   Size:       1949     732     192    1977    3399     345    1863    1464

Cuts at:  12214   12571   13549   14464   14956   15218     293 
   Size:        357     978     915     492     262    1469

  Fragments arranged by size: 

                3399    1977    1949    1863    1469    1464     978     915
                 732     492     357     345     262     192
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:      16394
 
SphI G_CATG'C

Cuts at:    498    1992    7986   13447   13947     498 
   Size:       1494    5994    5461     500    2945

  Fragments arranged by size: 

                5994    5461    2945    1494     500
 
StuI AGG'CCT

Cuts at:   3551    4369    4610    6403   12436    3551 
   Size:        818     241    1793    6033    7509

  Fragments arranged by size: 

                7509    6033    1793     818     241
 
StyI C'CwwG_G

Cuts at:    260    1169    2651    3758    3884    4112    4479    5618    5888 
   Size:        909    1482    1107     126     228     367    1139     270

Cuts at:   5888    6302    6473    6486    7040    7505    7697    7735    7967 
   Size:        414     171      13     554     465     192      38     232

Cuts at:   7967    8207    8789    8831    9713    9776   10215   11541   12202 
   Size:        240     582      42     882      63     439    1326     661

Cuts at:  12202   12368   13082   13232   13528   13882     260 
   Size:        166     714     150     296     354    2772

  Fragments arranged by size: 

                2772    1482    1326    1139    1107     909     882     714
                 661     582     554     465     439     414     367     354
                 296     270     240     232     228     192     171     166
                 150     126      63      42      38      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1746    2772    3962    4612    4619    5022    5551    5773    8587 
   Size:       1026    1190     650       7     403     529     222    2814

Cuts at:   8587    9057    9693   11766   11915   12546   13304   13977   14110 
   Size:        470     636    2073     149     631     758     673     133

Cuts at:  14110   14569   14778    1746 
   Size:        459     209    3362

  Fragments arranged by size: 

                3362    2814    2073    1190    1026     758     673     650
                 636     631     529     470     459     403     222     209
                 149     133       7
 
TatI w'GTAC_w

Cuts at:    501    2469    2576    4460    4883    5411    5630    5975    6947 
   Size:       1968     107    1884     423     528     219     345     972

Cuts at:   6947    6977    7667    7892   13241   15909     501 
   Size:         30     690     225    5349    2668     986

  Fragments arranged by size: 

                5349    2668    1968    1884     986     972     690     528
                 423     345     225     219     107      30
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    370     402    1003    1035    1064    1096    2794    2826    5835 
   Size:         32     601      32      29      32    1698      32    3009

Cuts at:   5835    5867    6552    6584    6610    6642    9319    9351   10403 
   Size:         32     685      32      26      32    2677      32    1052

Cuts at:  10403   10435   11692   11724   11762   11794   15911   15943     370 
   Size:         32    1257      32      38      32    4117      32     821

  Fragments arranged by size: 

                4117    3009    2677    1698    1257    1052     821     685
                 601      38      32      32      32      32      32      32
                  32      32      32      32      32      32      29      26
            
 
Tth111I GACn'n_nGTC

Cuts at:   1786    3442    4060    4531    8550   14191    1786 
   Size:       1656     618     471    4019    5641    3989

  Fragments arranged by size: 

                5641    4019    3989    1656     618     471
 
XbaI T'CTAG_A

Cuts at:   9369   13425    9369 
   Size:       4056   12338
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1884    6028    6351    7347    7863    9051    9207    9408   11288 
   Size:       4144     323     996     516    1188     156     201    1880

Cuts at:  11288   12459   12860   13889    1884 
   Size:       1171     401    1029    4389

  Fragments arranged by size: 

                4389    4144    1880    1188    1171    1029     996     516
                 401     323     201     156
 
XhoI C'TCGA_G

Cuts at:   8887    8887 
   Size:      16394
 
XmaI C'CCGG_G

Cuts at:   2235    5264    8693   12554   13414    2235 
   Size:       3029    3429    3861     860    5215

  Fragments arranged by size: 

                5215    3861    3429    3029     860
 
XmnI GAAnn'nnTTC

Cuts at:   2335    3208    4474    4652    5924    6110    2335 
   Size:        873    1266     178    1272     186   12619

  Fragments arranged by size: 

               12619    1272    1266     873     186     178
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NruI     NsiI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI   PspOMI    PspXI     PstI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AscI     FseI     HpaI     NotI     PacI     PmlI    PshAI
     PsiI     PsrI    SacII    SgrAI    SnaBI     SrfI     SspI     SwaI
     ZraI
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