Restriction map for pF1KB0497
 (Linear) (Six-base) MAPSORT of: KIBB0497.seq  Check: 5545  from: 1  to: 13281



KIBB0497.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2659    5390    6591    8586   11029   11730   13281 
   Size:       2659    2731    1201    1995    2443     701    1551

  Fragments arranged by size: 

                2731    2659    2443    1995    1551    1201     701
 
AccI GT'mk_AC

Cuts at:      0     416    2193    2312    4231    6674    7178    7406    8810 
   Size:        416    1777     119    1919    2443     504     228    1404

Cuts at:   8810   11009   11293   12661   13281 
   Size:       2199     284    1368     620

  Fragments arranged by size: 

                2443    2199    1919    1777    1404    1368     620     504
                 416     284     228     119
 
AclI AA'CG_TT

Cuts at:      0   12902   13281 
   Size:      12902     379
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391    1045    3181    3262    3433    3643    4052    6032 
   Size:        391     654    2136      81     171     210     409    1980

Cuts at:   6032    7705    8110    8155    8679    9526    9827    9841   10015 
   Size:       1673     405      45     524     847     301      14     174

Cuts at:  10015   10261   10303   12869   13281 
   Size:        246      42    2566     412

  Fragments arranged by size: 

                2566    2136    1980    1673     847     654     524     412
                 409     405     391     301     246     210     174     171
                  81      45      42      14
 
AfeI AGC'GCT

Cuts at:      0    7804   11587   13281 
   Size:       7804    3783    1694

  Fragments arranged by size: 

                7804    3783    1694
 
AflII C'TTAA_G

Cuts at:      0   12106   13281 
   Size:      12106    1175
 
AflIII A'CryG_T

Cuts at:      0    2879    3536    7331    7472    7781    8640   11872   12141 
   Size:       2879     657    3795     141     309     859    3232     269

Cuts at:  12141   13281 
   Size:       1140

  Fragments arranged by size: 

                3795    3232    2879    1140     859     657     309     269
                 141
 
AgeI A'CCGG_T

Cuts at:      0   12227   13281 
   Size:      12227    1054
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1811    5505    7615   13212   13281 
   Size:       1811    3694    2110    5597      69

  Fragments arranged by size: 

                5597    3694    2110    1811      69
 
AleI CACnn'nnGTG

Cuts at:      0   10753   12871   13281 
   Size:      10753    2118     410

  Fragments arranged by size: 

               10753    2118     410
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     125    4411    6607    9613   10582   11371   12362   13225 
   Size:        125    4286    2196    3006     969     789     991     863

Cuts at:  13225   13281 
   Size:         56

  Fragments arranged by size: 

                4286    3006    2196     991     969     863     789     125
                  56
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     820     852    6520    6552   13281 
   Size:        820      32    5668      32    6729

  Fragments arranged by size: 

                6729    5668     820      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1419    3201    3666    4206    4436    4660    5616    5898 
   Size:       1419    1782     465     540     230     224     956     282

Cuts at:   5898    7296    8310    8702    8997    9237    9897   10203   10239 
   Size:       1398    1014     392     295     240     660     306      36

Cuts at:  10239   10539   10554   13281 
   Size:        300      15    2727

  Fragments arranged by size: 

                2727    1782    1419    1398    1014     956     660     540
                 465     392     306     300     295     282     240     230
                 224      36      15
 
ApaI G_GGCC'C

Cuts at:      0     496    4730    7597    9303   12813   13281 
   Size:        496    4234    2867    1706    3510     468

  Fragments arranged by size: 

                4234    3510    2867    1706     496     468
 
ApaLI G'TGCA_C

Cuts at:      0    1651    1741   13281 
   Size:       1651      90   11540

  Fragments arranged by size: 

               11540    1651      90
 
ApoI r'AATT_y

Cuts at:      0     810    2326    2641    3430    3502    5147    8548   13281 
   Size:        810    1516     315     789      72    1645    3401    4733

  Fragments arranged by size: 

                4733    3401    1645    1516     810     789     315      72
            
 
AvaI C'yCGr_G

Cuts at:      0      77     214     245    1139    2127    4761    5156    5316 
   Size:         77     137      31     894     988    2634     395     160

Cuts at:   5316    6595    6835    8261    8585    8779   10157   12446   13281 
   Size:       1279     240    1426     324     194    1378    2289     835

  Fragments arranged by size: 

                2634    2289    1426    1378    1279     988     894     835
                 395     324     240     194     160     137      77      31
            
 
AvrII C'CTAG_G

Cuts at:      0    4371    6378   13281 
   Size:       4371    2007    6903

  Fragments arranged by size: 

                6903    4371    2007
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    6831    6864   12094   12127   13281 
   Size:       6831      33    5230      33    1154

  Fragments arranged by size: 

                6831    5230    1154      33      33
 
BamHI G'GATC_C

Cuts at:      0    6488    7931    8688    8705   11748   13281 
   Size:       6488    1443     757      17    3043    1533

  Fragments arranged by size: 

                6488    3043    1533    1443     757      17
 
BanI G'GyrC_C

Cuts at:      0     945    1240    3134    3318    5787    6825    7167    7699 
   Size:        945     295    1894     184    2469    1038     342     532

Cuts at:   7699    8523    8598   11282   12164   13281 
   Size:        824      75    2684     882    1117

  Fragments arranged by size: 

                2684    2469    1894    1117    1038     945     882     824
                 532     342     295     184      75
 
BanII G_rGCy'C

Cuts at:      0     149     215     496    1616    2265    3680    4730    5398 
   Size:        149      66     281    1120     649    1415    1050     668

Cuts at:   5398    6755    7039    7597    8099    8435    9303    9413    9644 
   Size:       1357     284     558     502     336     868     110     231

Cuts at:   9644   12813   13058   13281 
   Size:       3169     245     223

  Fragments arranged by size: 

                3169    1415    1357    1120    1050     868     668     649
                 558     502     336     284     281     245     231     223
                 149     110      66
 
BbeI G_GCGC'C

Cuts at:      0     949    3138   12168   13281 
   Size:        949    2189    9030    1113

  Fragments arranged by size: 

                9030    2189    1113     949
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1370    2418    2709    3369    3748    4385    7270    7944 
   Size:       1370    1048     291     660     379     637    2885     674

Cuts at:   7944    8117    8143   12875   13281 
   Size:        173      26    4732     406

  Fragments arranged by size: 

                4732    2885    1370    1048     674     660     637     406
                 379     291     173      26
 
BbvCI CC'TCA_GC

Cuts at:      0    1428    6406    6743    8509    8674    9704   10494   13281 
   Size:       1428    4978     337    1766     165    1030     790    2787

  Fragments arranged by size: 

                4978    2787    1766    1428    1030     790     337     165
            
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3277    3311    5565    5599    6136    6170   11254   11288 
   Size:       3277      34    2254      34     537      34    5084      34

Cuts at:  11288   13281 
   Size:       1993

  Fragments arranged by size: 

                5084    3277    2254    1993     537      34      34      34
                  34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1342    1836    3526    6666   10297   12977   13281 
   Size:       1342     494    1690    3140    3631    2680     304

  Fragments arranged by size: 

                3631    3140    2680    1690    1342     494     304
 
BclI T'GATC_A

Cuts at:      0    1091    1421    3981    4952    7433    7706    9467   13233 
   Size:       1091     330    2560     971    2481     273    1761    3766

Cuts at:  13233   13281 
   Size:         48

  Fragments arranged by size: 

                3766    2560    2481    1761    1091     971     330     273
                  48
 
BfrBI ATG'CAT

Cuts at:      0     854    7830   13281 
   Size:        854    6976    5451

  Fragments arranged by size: 

                6976    5451     854
 
BglI GCCn_nnn'nGGC

Cuts at:      0      79    5414    6646    8320    9298   10837   12808   13281 
   Size:         79    5335    1232    1674     978    1539    1971     473

  Fragments arranged by size: 

                5335    1971    1674    1539    1232     978     473      79
            
 
BglII A'GATC_T

Cuts at:      0     545    2891    4058    6084    8852    9258   10019   13281 
   Size:        545    2346    1167    2026    2768     406     761    3262

  Fragments arranged by size: 

                3262    2768    2346    2026    1167     761     545     406
            
 
BlpI GC'TnA_GC

Cuts at:      0     143    1985    5218    9674   11776   12061   13281 
   Size:        143    1842    3233    4456    2102     285    1220

  Fragments arranged by size: 

                4456    3233    2102    1842    1220     285     143
 
Bme1580I G_kGCm'C

Cuts at:      0     496     653     786    1245    1655    1745    4730    5937 
   Size:        496     157     133     459     410      90    2985    1207

Cuts at:   5937    6830    7172    7597    8192    8528    9303   10176   11550 
   Size:        893     342     425     595     336     775     873    1374

Cuts at:  11550   12813   13255   13281 
   Size:       1263     442      26

  Fragments arranged by size: 

                2985    1374    1263    1207     893     873     775     595
                 496     459     442     425     410     342     336     157
                 133      90      26
 
BmgBI CAC'GTC

Cuts at:      0    1452    8444   11875   13281 
   Size:       1452    6992    3431    1406

  Fragments arranged by size: 

                6992    3431    1452    1406
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1620    1947    2157    3220    3726    3855    3954    4293 
   Size:       1620     327     210    1063     506     129      99     339

Cuts at:   4293    5247    7268   11211   11676   12165   13281 
   Size:        954    2021    3943     465     489    1116

  Fragments arranged by size: 

                3943    2021    1620    1116    1063     954     506     489
                 465     339     327     210     129      99
 
BmtI G_CTAG'C

Cuts at:      0   12535   13281 
   Size:      12535     746
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     664     844    1294    1630    3163    3223    3361    3673 
   Size:        664     180     450     336    1533      60     138     312

Cuts at:   3673    3688    4648    5564    6745    7287    7516    9103    9325 
   Size:         15     960     916    1181     542     229    1587     222

Cuts at:   9325    9763    9847    9919   10561   10609   10672   10789   10984 
   Size:        438      84      72     642      48      63     117     195

Cuts at:  10984   11503   12262   12455   13281 
   Size:        519     759     193     826

  Fragments arranged by size: 

                1587    1533    1181     960     916     826     759     664
                 642     542     519     450     438     336     312     229
                 222     195     193     180     138     117      84      72
                  63      60      48      15
 
Bpu10I CC'TnA_GC

Cuts at:      0     164    1085    1428    3115    6406    6743    7466    8509 
   Size:        164     921     343    1687    3291     337     723    1043

Cuts at:   8509    8514    8674    9704   10494   10583   13281 
   Size:          5     160    1030     790      89    2698

  Fragments arranged by size: 

                3291    2698    1687    1043    1030     921     790     723
                 343     337     164     160      89       5
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170    2119    2851    3043    5020    8419   10627   12484 
   Size:        170    1949     732     192    1977    3399    2208    1857

Cuts at:  12484   13281 
   Size:        797

  Fragments arranged by size: 

                3399    2208    1977    1949    1857     797     732     192
                 170
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     730    3775    4567    5425    6130    6317   10483   10765 
   Size:        730    3045     792     858     705     187    4166     282

Cuts at:  10765   10774   12652   13281 
   Size:          9    1878     629

  Fragments arranged by size: 

                4166    3045    1878     858     792     730     705     629
                 282     187       9
 
BsaAI yAC'GTr

Cuts at:      0    1620    1971    7410   11131   13281 
   Size:       1620     351    5439    3721    2150

  Fragments arranged by size: 

                5439    3721    2150    1620     351
 
BsaBI GATnn'nnATC

Cuts at:      0   11368   11753   13281 
   Size:      11368     385    1528

  Fragments arranged by size: 

               11368    1528     385
 
BsaHI Gr'CG_yC

Cuts at:      0     946    1665    1960    3135    8301   10124   12165   13281 
   Size:        946     719     295    1175    5166    1823    2041    1116

  Fragments arranged by size: 

                5166    2041    1823    1175    1116     946     719     295
            
 
BsaWI w'CCGG_w

Cuts at:      0    1633    4481    4874   12227   13025   13281 
   Size:       1633    2848     393    7353     798     256

  Fragments arranged by size: 

                7353    2848    1633     798     393     256
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     820     850    2896    2926    6520    6550   10114   10144 
   Size:        820      30    2046      30    3594      30    3564      30

Cuts at:  10144   11422   11452   12904   12934   13171   13201   13281 
   Size:       1278      30    1452      30     237      30      80

  Fragments arranged by size: 

                3594    3564    2046    1452    1278     820     237      80
                  30      30      30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     172     841    3025    3736    7006    9142    9802    9895 
   Size:        172     669    2184     711    3270    2136     660      93

Cuts at:   9895    9916   10606   11629   12100   13281 
   Size:         21     690    1023     471    1181

  Fragments arranged by size: 

                3270    2184    2136    1181    1023     711     690     669
                 660     471     172      93      21
 
BseYI C'CCAG_C

Cuts at:      0     381    1199    1646    3130    4729    6906    7484    8056 
   Size:        381     818     447    1484    1599    2177     578     572

Cuts at:   8056    8422    8718    8976    9357    9530    9835   10547   10554 
   Size:        366     296     258     381     173     305     712       7

Cuts at:  10554   10832   11582   13019   13281 
   Size:        278     750    1437     262

  Fragments arranged by size: 

                2177    1599    1484    1437     818     750     712     578
                 572     447     381     381     366     305     296     278
                 262     258     173       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     493     586     904    1288    2128    2230    2368    3022 
   Size:        493      93     318     384     840     102     138     654

Cuts at:   3022    3094    3133    4357    4684    5368    5911    6237    6482 
   Size:         72      39    1224     327     684     543     326     245

Cuts at:   6482    7381    8641    8665    8718    9169   10348   10729   13281 
   Size:        899    1260      24      53     451    1179     381    2552

  Fragments arranged by size: 

                2552    1260    1224    1179     899     840     684     654
                 543     493     451     384     381     327     326     318
                 245     138     102      93      72      53      39      24
            
 
BsiEI CG_ry'CG

Cuts at:      0    9561   13281 
   Size:       9561    3720
 
BsiHKAI G_wGCw'C

Cuts at:      0     149    1655    1704    1745    3680    4489    7322    8207 
   Size:        149    1506      49      41    1935     809    2833     885

Cuts at:   8207    8435    8669   10862   11570   13281 
   Size:        228     234    2193     708    1711

  Fragments arranged by size: 

                2833    2193    1935    1711    1506     885     809     708
                 234     228     149      49      41
 
BsiWI C'GTAC_G

Cuts at:      0    7540   13281 
   Size:       7540    5741
 
BsmI GAATG_Cn'

Cuts at:      0     114    1829    2006    6284   13281 
   Size:        114    1715     177    4278    6997

  Fragments arranged by size: 

                6997    4278    1715     177     114
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1951    9570   13281 
   Size:       1951    7619    3711

  Fragments arranged by size: 

                7619    3711    1951
 
Bsp1286I G_dGCh'C

Cuts at:      0     149     215     496     653     786    1245    1616    1655 
   Size:        149      66     281     157     133     459     371      39

Cuts at:   1655    1704    1745    2265    3680    4489    4730    5398    5937 
   Size:         49      41     520    1415     809     241     668     539

Cuts at:   5937    6755    6830    7039    7172    7322    7597    8099    8192 
   Size:        818      75     209     133     150     275     502      93

Cuts at:   8192    8207    8435    8528    8669    9303    9413    9644   10176 
   Size:         15     228      93     141     634     110     231     532

Cuts at:  10176   10862   11550   11570   12813   13058   13255   13281 
   Size:        686     688      20    1243     245     197      26

  Fragments arranged by size: 

                1415    1243     818     809     688     686     668     634
                 539     532     520     502     459     371     281     275
                 245     241     231     228     209     197     157     150
                 149     141     133     133     110      93      93      75
                  66      49      41      39      26      20      15
 
BspEI T'CCGG_A

Cuts at:      0    1633    4481    4874   13281 
   Size:       1633    2848     393    8407

  Fragments arranged by size: 

                8407    2848    1633     393
 
BspHI T'CATG_A

Cuts at:      0    1052    1682    2405    5042   11249   13265   13281 
   Size:       1052     630     723    2637    6207    2016      16

  Fragments arranged by size: 

                6207    2637    2016    1052     723     630      16
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1696    2009    2390    2659    3923    4631    5390    6591 
   Size:       1696     313     381     269    1264     708     759    1201

Cuts at:   6591    8586    8663   10262   10280   11029   11730   11808   11931 
   Size:       1995      77    1599      18     749     701      78     123

Cuts at:  11931   13217   13281 
   Size:       1286      64

  Fragments arranged by size: 

                1995    1696    1599    1286    1264    1201     759     749
                 708     701     381     313     269     123      78      77
                  64      18
 
BsrBI CCG'CTC

Cuts at:      0    1944    5016   10578   12714   13281 
   Size:       1944    3072    5562    2136     567

  Fragments arranged by size: 

                5562    3072    2136    1944     567
 
BsrDI GCAATG_nn'

Cuts at:      0    1849    2969    3090    4109    5374    5804    6641   11355 
   Size:       1849    1120     121    1019    1265     430     837    4714

Cuts at:  11355   12116   13281 
   Size:        761    1165

  Fragments arranged by size: 

                4714    1849    1265    1165    1120    1019     837     761
                 430     121
 
BsrFI r'CCGG_y

Cuts at:      0    3749    5321    5641    7961   10687   12121   12161   12227 
   Size:       3749    1572     320    2320    2726    1434      40      66

Cuts at:  12227   13281 
   Size:       1054

  Fragments arranged by size: 

                3749    2726    2320    1572    1434    1054     320      66
                  40
 
BsrGI T'GTAC_A

Cuts at:      0     393    2361    2468    5867    7784   13281 
   Size:        393    1968     107    3399    1917    5497

  Fragments arranged by size: 

                5497    3399    1968    1917     393     107
 
BssHII G'CGCG_C

Cuts at:      0    1642   13281 
   Size:       1642   11639
 
BssSI C'ACGA_G

Cuts at:      0    3229    3286    3710    9844   11273   11509   13281 
   Size:       3229      57     424    6134    1429     236    1772

  Fragments arranged by size: 

                6134    3229    1772    1429     424     236      57
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     851    1311    1464    2463    7296    8202    8664    8997 
   Size:        851     460     153     999    4833     906     462     333

Cuts at:   8997   11364   13281 
   Size:       2367    1917

  Fragments arranged by size: 

                4833    2367    1917     999     906     851     462     460
                 333     153
 
BstBI TT'CG_AA

Cuts at:      0    1785    3500    6770   11036   13281 
   Size:       1785    1715    3270    4266    2245

  Fragments arranged by size: 

                4266    3270    2245    1785    1715
 
BstEII G'GTnAC_C

Cuts at:      0    2397    4854    8361   13281 
   Size:       2397    2457    3507    4920

  Fragments arranged by size: 

                4920    3507    2457    2397
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3528    3579    4720    5665    6246    7239    8946   11082 
   Size:       3528      51    1141     945     581     993    1707    2136

Cuts at:  11082   11333   12780   13281 
   Size:        251    1447     501

  Fragments arranged by size: 

                3528    2136    1707    1447    1141     993     945     581
                 501     251      51
 
BstYI r'GATC_y

Cuts at:      0     545     971    1539    2120    2807    2891    3272    3965 
   Size:        545     426     568     581     687      84     381     693

Cuts at:   3965    4058    4967    5247    5612    5699    5985    6084    6488 
   Size:         93     909     280     365      87     286      99     404

Cuts at:   6488    7931    8121    8558    8688    8705    8852    9258   10019 
   Size:       1443     190     437     130      17     147     406     761

Cuts at:  10019   10661   11748   12491   12575   13281 
   Size:        642    1087     743      84     706

  Fragments arranged by size: 

                1443    1087     909     761     743     706     693     687
                 642     581     568     545     437     426     406     404
                 381     365     286     280     190     147     130      99
                  93      87      84      84      17
 
BstZ17I GTA'TAC

Cuts at:      0    2194    2313   11294   13281 
   Size:       2194     119    8981    1987

  Fragments arranged by size: 

                8981    2194    1987     119
 
Bsu36I CC'TnA_GG

Cuts at:      0    4894   10190   12323   13281 
   Size:       4894    5296    2133     958

  Fragments arranged by size: 

                5296    4894    2133     958
 
BtgI C'CryG_G

Cuts at:      0     169    3776    3785    3792    5369    5510    5780    6506 
   Size:        169    3607       9       7    1577     141     270     726

Cuts at:   6506    6932    7250    7589    7793    8681    9605   11241   13281 
   Size:        426     318     339     204     888     924    1636    2040

  Fragments arranged by size: 

                3607    2040    1636    1577     924     888     726     426
                 339     318     270     204     169     141       9       7
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2908    4847    6018    6570    6604    7257   10605   11332 
   Size:       2908    1939    1171     552      34     653    3348     727

Cuts at:  11332   11610   13281 
   Size:        278    1671

  Fragments arranged by size: 

                3348    2908    1939    1671    1171     727     653     552
                 278      34
 
BtsI GCAGTG_nn'

Cuts at:      0     281    2464    2527    3078    4955    9127    9271   12866 
   Size:        281    2183      63     551    1877    4172     144    3595

Cuts at:  12866   13253   13281 
   Size:        387      28

  Fragments arranged by size: 

                4172    3595    2183    1877     551     387     281     144
                  63      28
 
ClaI AT'CG_AT

Cuts at:      0   10061   13281 
   Size:      10061    3220
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    5787    5822   12117   12152   13281 
   Size:       5787      35    6295      35    1129

  Fragments arranged by size: 

                6295    5787    1129      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     196    1464    2186    4994    6518    8664   11670   11970 
   Size:        196    1268     722    2808    1524    2146    3006     300

Cuts at:  11970   13281 
   Size:       1311

  Fragments arranged by size: 

                3006    2808    2146    1524    1311    1268     722     300
                 196
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1014    8306   10609   13281 
   Size:       1014    7292    2303    2672

  Fragments arranged by size: 

                7292    2672    2303    1014
 
EaeI y'GGCC_r

Cuts at:      0      36      89    1600    3254    3581    3747    4941    5730 
   Size:         36      53    1511    1654     327     166    1194     789

Cuts at:   5730    5855    6647    6881    7217    9558   12333   12470   12770 
   Size:        125     792     234     336    2341    2775     137     300

Cuts at:  12770   13281 
   Size:        511

  Fragments arranged by size: 

                2775    2341    1654    1511    1194     792     789     511
                 336     327     300     234     166     137     125      53
                  36
 
EagI C'GGCC_G

Cuts at:      0    9558   13281 
   Size:       9558    3723
 
EarI CTCTTCn'nnn_

Cuts at:      0     241    1165    2327    2590    2958    3587    5993    6724 
   Size:        241     924    1162     263     368     629    2406     731

Cuts at:   6724    6997    7730    7843    8212    8549    8626    9260    9281 
   Size:        273     733     113     369     337      77     634      21

Cuts at:   9281    9803   12446   12668   13111   13281 
   Size:        522    2643     222     443     170

  Fragments arranged by size: 

                2643    2406    1162     924     733     731     634     629
                 522     443     369     368     337     273     263     241
                 222     170     113      77      21
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     283    5257    5758    7495    8698   11755   13281 
   Size:        283    4974     501    1737    1203    3057    1526

  Fragments arranged by size: 

                4974    3057    1737    1526    1203     501     283
 
EcoICRI GAG'CTC

Cuts at:      0     147    3678    8433   13281 
   Size:        147    3531    4755    4848

  Fragments arranged by size: 

                4848    4755    3531     147
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391     664     844    1045    1294    1630    3163    3181 
   Size:        391     273     180     201     249     336    1533      18

Cuts at:   3181    3223    3262    3361    3433    3643    3673    3688    4052 
   Size:         42      39      99      72     210      30      15     364

Cuts at:   4052    4648    5564    6032    6745    7287    7516    7705    8110 
   Size:        596     916     468     713     542     229     189     405

Cuts at:   8110    8155    8679    9103    9325    9526    9763    9827    9841 
   Size:         45     524     424     222     201     237      64      14

Cuts at:   9841    9847    9919   10015   10261   10303   10561   10609   10672 
   Size:          6      72      96     246      42     258      48      63

Cuts at:  10672   10789   10984   11503   12262   12455   12869   13281 
   Size:        117     195     519     759     193     414     412

  Fragments arranged by size: 

                1533     916     759     713     596     542     524     519
                 468     424     414     412     405     391     364     336
                 273     258     249     246     237     229     222     210
                 201     201     195     193     189     180     117      99
                  96      72      72      64      63      48      45      42
                  42      39      30      18      15      14       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1485    2386    3670    5449    6193    8779    9286    9901 
   Size:       1485     901    1284    1779     744    2586     507     615

Cuts at:   9901   11386   11608   11644   12568   13281 
   Size:       1485     222      36     924     713

  Fragments arranged by size: 

                2586    1779    1485    1485    1284     924     901     744
                 713     615     507     222      36
 
EcoO109I rG'GnC_Cy

Cuts at:      0     181     321     492     493     817    1219    1395    1998 
   Size:        181     140     171       1     324     402     176     603

Cuts at:   1998    2082    2319    3654    4726    4810    4866    5407    5442 
   Size:         84     237    1335    1072      84      56     541      35

Cuts at:   5442    5454    5493    6627    7246    7481    7594    8772    9300 
   Size:         12      39    1134     619     235     113    1178     528

Cuts at:   9300    9518    9894    9906    9964   10200   10236   10536   11619 
   Size:        218     376      12      58     236      36     300    1083

Cuts at:  11619   11853   12459   12996   13281 
   Size:        234     606     537     285

  Fragments arranged by size: 

                1335    1178    1134    1083    1072     619     606     603
                 541     537     528     402     376     324     300     285
                 237     236     235     234     218     181     176     171
                 140     113      84      84      58      56      39      36
                  35      12      12       1
 
EcoRI G'AATT_C

Cuts at:      0    2641    3502   13281 
   Size:       2641     861    9779

  Fragments arranged by size: 

                9779    2641     861
 
EcoRV GAT'ATC

Cuts at:      0    8400    9552   13281 
   Size:       8400    1152    3729

  Fragments arranged by size: 

                8400    3729    1152
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     252    2931    3267   13281 
   Size:        252    2679     336   10014

  Fragments arranged by size: 

               10014    2679     336     252
 
FspI TGC'GCA

Cuts at:      0    2005   10405   13281 
   Size:       2005    8400    2876

  Fragments arranged by size: 

                8400    2876    2005
 
FspAI rTGC'GCAy

Cuts at:      0   10405   13281 
   Size:      10405    2876
 
HaeII r_GCGC'y

Cuts at:      0     949    2937    3138    5973    7134    7806   11589   12168 
   Size:        949    1988     201    2835    1161     672    3783     579

Cuts at:  12168   13124   13281 
   Size:        956     157

  Fragments arranged by size: 

                3783    2835    1988    1161     956     949     672     579
                 201     157
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     615     647    1792    1797    1824    1829    3885    3917 
   Size:        615      32    1145       5      27       5    2056      32

Cuts at:   3917    3973    4005    4367    4399    4476    4508    5491    5523 
   Size:         56      32     362      32      77      32     983      32

Cuts at:   5523    5578    5610    6589    6621    7601    7633    7894    7926 
   Size:         55      32     979      32     980      32     261      32

Cuts at:   7926    8384    8416    8548    8580    9325    9357    9475    9507 
   Size:        458      32     132      32     745      32     118      32

Cuts at:   9507   10114   10146   10173   10205   11492   11524   11697   11729 
   Size:        607      32      27      32    1287      32     173      32

Cuts at:  11729   12054   12086   12343   12375   12904   12936   13171   13198 
   Size:        325      32     257      32     529      32     235      27

Cuts at:  13198   13203   13218   13230   13250   13281 
   Size:          5      15      12      20      31

  Fragments arranged by size: 

                2056    1287    1145     983     980     979     745     615
                 607     529     458     362     325     261     257     235
                 173     132     118      77      56      55      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      31      27      27      27      20
                  15      12       5       5       5
 
HincII GTy'rAC

Cuts at:      0    1119    1893    4428    5481    6563    7179    7329    9999 
   Size:       1119     774    2535    1053    1082     616     150    2670

Cuts at:   9999   10515   13281 
   Size:        516    2766

  Fragments arranged by size: 

                2766    2670    2535    1119    1082    1053     774     616
                 516     150
 
HindIII A'AGCT_T

Cuts at:      0    3996    5173    7717   13281 
   Size:       3996    1177    2544    5564

  Fragments arranged by size: 

                5564    3996    2544    1177
 
KasI G'GCGC_C

Cuts at:      0     945    3134   12164   13281 
   Size:        945    2189    9030    1117

  Fragments arranged by size: 

                9030    2189    1117     945
 
MfeI C'AATT_G

Cuts at:      0    5774   12684   13281 
   Size:       5774    6910     597

  Fragments arranged by size: 

                6910    5774     597
 
MluI A'CGCG_T

Cuts at:      0    8640   13281 
   Size:       8640    4641
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     155    1820    3405    4701    5147    7075    8885    9056 
   Size:        155    1665    1585    1296     446    1928    1810     171

Cuts at:   9056   10493   12059   13281 
   Size:       1437    1566    1222

  Fragments arranged by size: 

                1928    1810    1665    1585    1566    1437    1296    1222
                 446     171     155
 
MscI TGG'CCA

Cuts at:      0      91    1602    3256    3583    4943    5857    6883    7219 
   Size:         91    1511    1654     327    1360     914    1026     336

Cuts at:   7219   12335   12772   13281 
   Size:       5116     437     509

  Fragments arranged by size: 

                5116    1654    1511    1360    1026     914     509     437
                 336     327      91
 
MslI CAynn'nnrTG

Cuts at:      0     685    2390    3083    3577    4852    5587    5663    5710 
   Size:        685    1705     693     494    1275     735      76      47

Cuts at:   5710    5785    6244    6589    7441    7825    8455    8924    9145 
   Size:         75     459     345     852     384     630     469     221

Cuts at:   9145    9595   10564   10753   11069   11575   11830   12344   12595 
   Size:        450     969     189     316     506     255     514     251

Cuts at:  12595   12655   12778   12871   13281 
   Size:         60     123      93     410

  Fragments arranged by size: 

                1705    1275     969     852     735     693     685     630
                 514     506     494     469     459     450     410     384
                 345     316     255     251     221     189     123      93
                  76      75      60      47
 
MspA1I CmG'CkG

Cuts at:      0     882    1199    2359    2828    3972    4395    5898    6263 
   Size:        882     317    1160     469    1144     423    1503     365

Cuts at:   6263    6662    6845    6910    8297    9943   10551   10713   10767 
   Size:        399     183      65    1387    1646     608     162      54

Cuts at:  10767   10836   11559   11736   11912   12475   13281 
   Size:         69     723     177     176     563     806

  Fragments arranged by size: 

                1646    1503    1387    1160    1144     882     806     723
                 608     563     469     423     399     365     317     183
                 177     176     162      69      65      54
 
NaeI GCC'GGC

Cuts at:      0    5323    5643   12123   13281 
   Size:       5323     320    6480    1158

  Fragments arranged by size: 

                6480    5323    1158     320
 
NarI GG'CG_CC

Cuts at:      0     946    3135   12165   13281 
   Size:        946    2189    9030    1116

  Fragments arranged by size: 

                9030    2189    1116     946
 
NcoI C'CATG_G

Cuts at:      0    3776    5510    5780    6932    7589    8681    9605   13281 
   Size:       3776    1734     270    1152     657    1092     924    3676

  Fragments arranged by size: 

                3776    3676    1734    1152    1092     924     657     270
            
 
NdeI CA'TA_TG

Cuts at:      0   11066   13281 
   Size:      11066    2215
 
NgoMIV G'CCGG_C

Cuts at:      0    5321    5641   12121   13281 
   Size:       5321     320    6480    1160

  Fragments arranged by size: 

                6480    5321    1160     320
 
NheI G'CTAG_C

Cuts at:      0   12531   13281 
   Size:      12531     750
 
NruI TCG'CGA

Cuts at:      0    3106    6589   13281 
   Size:       3106    3483    6692

  Fragments arranged by size: 

                6692    3483    3106
 
NsiI A_TGCA'T

Cuts at:      0     856    7832   13281 
   Size:        856    6976    5449

  Fragments arranged by size: 

                6976    5449     856
 
NspI r_CATG'y

Cuts at:      0     390    1551    1884    2200    2883    3540    4042    6461 
   Size:        390    1161     333     316     683     657     502    2419

Cuts at:   6461    6768    7335    7476    7785    7878    8274    9594   12145 
   Size:        307     567     141     309      93     396    1320    2551

Cuts at:  12145   13134   13281 
   Size:        989     147

  Fragments arranged by size: 

                2551    2419    1320    1161     989     683     657     567
                 502     396     390     333     316     309     307     147
                 141      93
 
PasI CC'CwG_GG

Cuts at:      0    1245    5457    7600   11604   13281 
   Size:       1245    4212    2143    4004    1677

  Fragments arranged by size: 

                4212    4004    2143    1677    1245
 
PciI A'CATG_T

Cuts at:      0    2879    3536    7331    7472    7781   12141   13281 
   Size:       2879     657    3795     141     309    4360    1140

  Fragments arranged by size: 

                4360    3795    2879    1140     657     309     141
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     449    1044    1971    2255    2394    4121    4206    6419 
   Size:        449     595     927     284     139    1727      85    2213

Cuts at:   6419    6895    7310    8310    8747    9897   10554   12768   13281 
   Size:        476     415    1000     437    1150     657    2214     513

  Fragments arranged by size: 

                2214    2213    1727    1150    1000     927     657     595
                 513     476     449     437     415     284     139      85
            
 
PfoI T'CCnGG_A

Cuts at:      0     974    1334    2108    3140    3338    3956    4505    4535 
   Size:        974     360     774    1032     198     618     549      30

Cuts at:   4535   10091   13281 
   Size:       5556    3190

  Fragments arranged by size: 

                5556    3190    1032     974     774     618     549     360
                 198      30
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     820     852    4280    4312    6520    6552   13281 
   Size:        820      32    3428      32    2208      32    6729

  Fragments arranged by size: 

                6729    3428    2208     820      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     181     321     817    1219    1395    1998    2319    3654 
   Size:        181     140     496     402     176     603     321    1335

Cuts at:   3654    4866    5442    6627    7246    7481    9906    9964   12459 
   Size:       1212     576    1185     619     235    2425      58    2495

Cuts at:  12459   13281 
   Size:        822

  Fragments arranged by size: 

                2495    2425    1335    1212    1185     822     619     603
                 576     496     402     321     235     181     176     140
                  58
 
PspOMI G'GGCC_C

Cuts at:      0     492    4726    7593    9299   12809   13281 
   Size:        492    4234    2867    1706    3510     472

  Fragments arranged by size: 

                4234    3510    2867    1706     492     472
 
PspXI vC'TCGA_Gb

Cuts at:      0    8779   13281 
   Size:       8779    4502
 
PstI C_TGCA'G

Cuts at:      0     140     675    2660    2858    3767    5415    7292   10259 
   Size:        140     535    1985     198     909    1648    1877    2967

Cuts at:  10259   11558   11741   11999   12572   12881   13104   13281 
   Size:       1299     183     258     573     309     223     177

  Fragments arranged by size: 

                2967    1985    1877    1648    1299     909     573     535
                 309     258     223     198     183     177     140
 
PvuII CAG'CTG

Cuts at:      0     882    2359    4395    5898    6910    9943   10551   10713 
   Size:        882    1477    2036    1503    1012    3033     608     162

Cuts at:  10713   10767   11559   11736   13281 
   Size:         54     792     177    1545

  Fragments arranged by size: 

                3033    2036    1545    1503    1477    1012     882     792
                 608     177     162      54
 
SacI G_AGCT'C

Cuts at:      0     149    3680    8435   13281 
   Size:        149    3531    4755    4846

  Fragments arranged by size: 

                4846    4755    3531     149
 
SalI G'TCGA_C

Cuts at:      0    7177   13281 
   Size:       7177    6104
 
SanDI GG'GwC_CC

Cuts at:      0    1219    7246    7481    9964   13281 
   Size:       1219    6027     235    2483    3317

  Fragments arranged by size: 

                6027    3317    2483    1219     235
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1165    8212   13111   13281 
   Size:       1165    7047    4899     170

  Fragments arranged by size: 

                7047    4899    1165     170
 
SbfI CC_TGCA'GG

Cuts at:      0    5415   13281 
   Size:       5415    7866
 
ScaI AGT'ACT

Cuts at:      0    5305   13281 
   Size:       5305    7976
 
SexAI A'CCwGG_T

Cuts at:      0    3950    4217    4421    5054    7199    9134    9917   13281 
   Size:       3950     267     204     633    2145    1935     783    3364

  Fragments arranged by size: 

                3950    3364    2145    1935     783     633     267     204
            
 
SfcI C'TryA_G

Cuts at:      0     136     326     671    2656    2854    3763    4088    5411 
   Size:        136     190     345    1985     198     909     325    1323

Cuts at:   5411    5977    6289    7288   10255   10373   10479   11010   11554 
   Size:        566     312     999    2967     118     106     531     544

Cuts at:  11554   11737   11995   12568   12877   13100   13281 
   Size:        183     258     573     309     223     181

  Fragments arranged by size: 

                2967    1985    1323     999     909     573     566     544
                 531     345     325     312     309     258     223     198
                 190     183     181     136     118     106
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    6646    9298   12808   13281 
   Size:       6646    2652    3510     473

  Fragments arranged by size: 

                6646    3510    2652     473
 
SfoI GGC'GCC

Cuts at:      0     947    3136   12166   13281 
   Size:        947    2189    9030    1115

  Fragments arranged by size: 

                9030    2189    1115     947
 
SmaI CCC'GGG

Cuts at:      0    2129    5158    8587   12448   13281 
   Size:       2129    3029    3429    3861     833

  Fragments arranged by size: 

                3861    3429    3029    2129     833
 
SmlI C'TyrA_G

Cuts at:      0     185    2134    2866    3058    5035    8434    8779   10642 
   Size:        185    1949     732     192    1977    3399     345    1863

Cuts at:  10642   12106   12463   13281 
   Size:       1464     357     818

  Fragments arranged by size: 

                3399    1977    1949    1863    1464     818     732     357
                 345     192     185
 
SphI G_CATG'C

Cuts at:      0     390    1884    7878   13281 
   Size:        390    1494    5994    5403

  Fragments arranged by size: 

                5994    5403    1494     390
 
StuI AGG'CCT

Cuts at:      0    3443    4261    4502    6295   12328   13281 
   Size:       3443     818     241    1793    6033     953

  Fragments arranged by size: 

                6033    3443    1793     953     818     241
 
StyI C'CwwG_G

Cuts at:      0     152    1061    2543    3650    3776    4004    4371    5510 
   Size:        152     909    1482    1107     126     228     367    1139

Cuts at:   5510    5780    6194    6365    6378    6932    7397    7589    7627 
   Size:        270     414     171      13     554     465     192      38

Cuts at:   7627    7859    8099    8681    8723    9605    9668   10107   11433 
   Size:        232     240     582      42     882      63     439    1326

Cuts at:  11433   12094   12260   12974   13124   13281 
   Size:        661     166     714     150     157

  Fragments arranged by size: 

                1482    1326    1139    1107     909     882     714     661
                 582     554     465     439     414     367     270     240
                 232     228     192     171     166     157     152     150
                 126      63      42      38      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1638    2664    3854    4504    4511    4914    5443    5665 
   Size:       1638    1026    1190     650       7     403     529     222

Cuts at:   5665    8479    8949    9585   11658   11807   12438   13196   13281 
   Size:       2814     470     636    2073     149     631     758      85

  Fragments arranged by size: 

                2814    2073    1638    1190    1026     758     650     636
                 631     529     470     403     222     149      85       7
            
 
TatI w'GTAC_w

Cuts at:      0     393    2361    2468    4352    4775    5303    5522    5867 
   Size:        393    1968     107    1884     423     528     219     345

Cuts at:   5867    6839    6869    7559    7784   13133   13281 
   Size:        972      30     690     225    5349     148

  Fragments arranged by size: 

                5349    1968    1884     972     690     528     423     393
                 345     225     219     148     107      30
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     262     294     895     927     956     988    2686    2718 
   Size:        262      32     601      32      29      32    1698      32

Cuts at:   2718    5727    5759    6444    6476    6502    6534    9211    9243 
   Size:       3009      32     685      32      26      32    2677      32

Cuts at:   9243   10295   10327   11584   11616   11654   11686   13281 
   Size:       1052      32    1257      32      38      32    1595

  Fragments arranged by size: 

                3009    2677    1698    1595    1257    1052     685     601
                 262      38      32      32      32      32      32      32
                  32      32      32      32      32      29      26
 
Tth111I GACn'n_nGTC

Cuts at:      0    1678    3334    3952    4423    8442   13281 
   Size:       1678    1656     618     471    4019    4839

  Fragments arranged by size: 

                4839    4019    1678    1656     618     471
 
XbaI T'CTAG_A

Cuts at:      0    9261   13281 
   Size:       9261    4020
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1776    5920    6243    7239    7755    8943    9099    9300 
   Size:       1776    4144     323     996     516    1188     156     201

Cuts at:   9300   11180   12351   12752   13281 
   Size:       1880    1171     401     529

  Fragments arranged by size: 

                4144    1880    1776    1188    1171     996     529     516
                 401     323     201     156
 
XhoI C'TCGA_G

Cuts at:      0    8779   13281 
   Size:       8779    4502
 
XmaI C'CCGG_G

Cuts at:      0    2127    5156    8585   12446   13281 
   Size:       2127    3029    3429    3861     835

  Fragments arranged by size: 

                3861    3429    3029    2127     835
 
XmnI GAAnn'nnTTC

Cuts at:      0    2227    3100    4366    4544    5816    6002   13281 
   Size:       2227     873    1266     178    1272     186    7279

  Fragments arranged by size: 

                7279    2227    1272    1266     873     186     178
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     KasI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NruI     NsiI     NspI     PasI     PciI
    PflMI     PfoI     PpiI    PpuMI   PspOMI    PspXI     PstI    PvuII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SmaI     SmlI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AscI     AseI    AsiSI     BplI     DraI     FseI
     HpaI     KpnI     NotI     PacI     PmeI     PmlI    PshAI     PsiI
     PsrI     PvuI    RsrII    SacII     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SspI     SwaI     ZraI
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