Restriction map for pF1KA1839
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# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspEI       AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BpmI        
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiWI       BsmAI       BsmBI       
BsmI        BspDI       BspEI       BsrBI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstNI       
BstPI       BstXI       BstZ17I     ClaI        CpoI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRII      
FbaI        FseI        FspI        HaeIII      HgaI        
Hin1I       HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        MvaI        NarI        NdeI        
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PstI        PvuI        RsrII       
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspI        StuI        
SwaI        Tsp45I      XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   65 enzymes) #
###################################

AccII         611  CGCG                 CG^CG
AflIII       1287  ACATGT               A^C[GA][CT]GT
AlwNI         338  CAGGCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          777  ATTAAT               AT^TAAT
AsnI          777  ATTAAT               AT^TAAT
Asp700I       141  GAAGTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          45  GAGCTC               G[AT]GC[AT]^C
AspI          367  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI           95  GGCACC               G^G[CT][GA]CC
BcnI         1243  CCGGG                CC^[GC]GG
BlpI          106  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      106  GCTGAGC              GC^T[ACGT]AGC
BsiEI          75  CGACCG               CG[GA][CT]^CG
BsiHKAI        45  GAGCTC               G[AT]GC[AT]^C
BsmFI         329  GTCCC                GGGAC(10/14)
Bsp1407I      549  TGTACA               T^GTACA
BspHI        1041  TCATGA               T^CATGA
BspLU11I     1287  ACATGT               A^CATGT
BsrDI         641  CATTGC               GCAATG(2/0)
BsrFI         102  GCCGGC               [GA]^CCGG[CT]
BsrGI         549  TGTACA               T^GTACA
BsrI          904  CCAGT                ACTGG(1/-1)
BssKI        1243  CCGGG                ^CC[ACGT]GG
BssSI          42  CACGAG               CACGAG(-5/-1)
BstUI         611  CGCG                 CG^CG
BstYI        1524  AGATCC               [GA]^GATC[CT]
CelII         106  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        102  GCCGGC               [GA]^CCGG[CT]
DraI          456  TTTAAA               TTT^AAA
EarI          653  CTCTTC               CTCTTC(1/4)
EcoNI        1528  CCTAAGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         964  GAATTC               G^AATTC
EcoRV          27  GATATC               GAT^ATC
EcoT22I      1067  ATGCAT               ATGCA^T
HaeII         827  AGCGCC               [GA]GCGC^[CT]
HincII       1330  GTTGAC               GT[CT]^[GA]AC
HindII       1330  GTTGAC               GT[CT]^[GA]AC
HindIII      1403  AAGCTT               A^AGCTT
HphI         1246  GGTGA                GGTGA(8/7)
Ksp632I       653  CTCTTC               CTCTTC(1/4)
MaeII        1371  ACGT                 A^CGT
MflI         1524  AGATCC               [GA]^GATC[CT]
MslI          872  CACCTTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          611  CGCG                 CG^CG
NaeI          102  GCCGGC               GCC^GGC
NciI         1243  CCGGG                CC^[GC]GG
NgoMIV        102  GCCGGC               G^CCGGC
NsiI         1067  ATGCAT               ATGCA^T
PflFI         367  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         433  CCAAACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI         777  ATTAAT               AT^TAAT
PvuII         989  CAGCTG               CAG^CTG
RcaI         1041  TCATGA               T^CATGA
SacI           45  GAGCTC               GAGCT^C
ScaI          143  AGTACT               AGT^ACT
ScrFI        1243  CCGGG                CC^[ACGT]GG
SmlI          469  CTCAAG               C^T[CT][GA]AG
SphI          645  GCATGC               GCATG^C
SspBI         549  TGTACA               T^GTACA
TfiI          727  GAATC                G^A[AT]TC
Tth111I       367  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        433  CCAAACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1100  CCAAACCTAATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1524  AGATCC               [GA]^GATC[CT]
XmnI          141  GAAGTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AlwI         1525  GATCC                GGATC(4/5)
             1355  GGATC               
AocI          679  CCTGAGG              CC^T[ACGT]AGG
             1528  CCTAAGG             
AvaI           10  CCCGAG               C^[CT]CG[GA]G
               68  CTCGGG              
BanII          45  GAGCTC               G[GA]GC[CT]^C
              152  GGGCTC              
BbsI          628  GAAGAC               GAAGAC(2/6)
             1135  GAAGAC              
BbvCI         670  CCTCAGC              CCTCAGC(-5/-2)
              118  GCTGAGG             
BmyI           45  GAGCTC               G[AGT]GC[ACT]^C
              152  GGGCTC              
BpuAI         628  GAAGAC               GAAGAC(2/6)
             1135  GAAGAC              
BsoBI          10  CCCGAG               C^[CT]CG[GA]G
               68  CTCGGG              
Bsp1286I       45  GAGCTC               G[AGT]GC[ACT]^C
              152  GGGCTC              
BspMI          98  ACCTGC               ACCTGC(4/8)
             1050  ACCTGC              
Bsu36I        679  CCTGAGG              CC^T[ACGT]AGG
             1528  CCTAAGG             
CfoI          610  GCGC                 GCG^C
              828  GCGC                
DsaI          438  CCATGG               C^C[GA][CT]GG
             1484  CCATGG              
Eco81I        679  CCTGAGG              CC^T[ACGT]AGG
             1528  CCTAAGG             
FauI           63  CCCGC                CCCGC(4/6)
              165  GCGGG               
HapII         103  CCGG                 C^CGG
             1243  CCGG                
HhaI          610  GCGC                 GCG^C
              828  GCGC                
HinP1I        610  GCGC                 G^CGC
              828  GCGC                
HpaII         103  CCGG                 C^CGG
             1243  CCGG                
MaeIII        912  GTTAC                ^GT[ACGT]AC
              950  GTAAC               
MspA1I        989  CAGCTG               C[AC]G^C[GT]G
             1351  CAGCGG              
MspI          103  CCGG                 C^CGG
             1243  CCGG                
NcoI          438  CCATGG               C^CATGG
             1484  CCATGG              
PleI          251  GACTC                GAGTC(4/5)
             1138  GACTC               
SfcI          944  CTGTAG               C^T[GA][CT]AG
             1257  CTGTAG              
TspRI         744  CACTG                CA[GC]TG[ACGT][ACGT]^
              905  CAGTG               
XbaI         1168  TCTAGA               T^CTAGA
             1198  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AvaII       BbvI        BfaI        BsaJI       BseRI       
BsiYI       BslI        BstF5I      Cac8I       Cfr13I      
DdeI        DpnII       DraII       EcoO109I    EcoT14I     
Fnu4HI      FokI        HinfI       ItaI        MaeI        
MboI        MboII       MnlI        MseI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PpuMI       
RsaI        Sau3AI      Sau96I      SfaNI       StyI        
TaqI        Tru9I       TseI        Tsp509I     TthHB8I     
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