Restriction map for pF1KA1816
 (Linear) (Six-base) MAPSORT of: KIAA1816.seq  Check: 6391  from: 1  to: 3399



KIAA1816.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

Acc65I G'GTAC_C

Cuts at:      0     281    3399 
   Size:        281    3118
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     623    3399 
   Size:        623    2776
 
AflIII A'CryG_T

Cuts at:      0    1101    1382    3257    3399 
   Size:       1101     281    1875     142

  Fragments arranged by size: 

                1875    1101     281     142
 
AleI CACnn'nnGTG

Cuts at:      0     217    3399 
   Size:        217    3182
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     551    3399 
   Size:        551    2848
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     690     722    3399 
   Size:        690      32    2677

  Fragments arranged by size: 

                2677     690      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     981    1036    1360    2262    3000    3399 
   Size:        981      55     324     902     738     399

  Fragments arranged by size: 

                 981     902     738     399     324      55
 
ApaI G_GGCC'C

Cuts at:      0     178    2232    3399 
   Size:        178    2054    1167

  Fragments arranged by size: 

                2054    1167     178
 
ApoI r'AATT_y

Cuts at:      0     549     572    1018    3399 
   Size:        549      23     446    2381

  Fragments arranged by size: 

                2381     549     446      23
 
AseI AT'TA_AT

Cuts at:      0     905     934    3399 
   Size:        905      29    2465

  Fragments arranged by size: 

                2465     905      29
 
AvaI C'yCGr_G

Cuts at:      0     402    3087    3399 
   Size:        402    2685     312

  Fragments arranged by size: 

                2685     402     312
 
AvrII C'CTAG_G

Cuts at:      0    2806    3399 
   Size:       2806     593
 
BanI G'GyrC_C

Cuts at:      0     257     281     376     382    2938    3100    3399 
   Size:        257      24      95       6    2556     162     299

  Fragments arranged by size: 

                2556     299     257     162      95      24       6
 
BanII G_rGCy'C

Cuts at:      0     178     504    2232    3399 
   Size:        178     326    1728    1167

  Fragments arranged by size: 

                1728    1167     326     178
 
BbeI G_GCGC'C

Cuts at:      0     380    2942    3399 
   Size:        380    2562     457

  Fragments arranged by size: 

                2562     457     380
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     998    1681    1988    3399 
   Size:        998     683     307    1411

  Fragments arranged by size: 

                1411     998     683     307
 
BclI T'GATC_A

Cuts at:      0     461     915    1718    2220    3399 
   Size:        461     454     803     502    1179

  Fragments arranged by size: 

                1179     803     502     461     454
 
BglI GCCn_nnn'nGGC

Cuts at:      0     182    1966    3399 
   Size:        182    1784    1433

  Fragments arranged by size: 

                1784    1433     182
 
BglII A'GATC_T

Cuts at:      0     723    3399 
   Size:        723    2676
 
Bme1580I G_kGCm'C

Cuts at:      0     178    1373    1971    2123    2232    3034    3399 
   Size:        178    1195     598     152     109     802     365

  Fragments arranged by size: 

                1195     802     598     365     178     152     109
 
BmgBI CAC'GTC

Cuts at:      0     264    3258    3399 
   Size:        264    2994     141

  Fragments arranged by size: 

                2994     264     141
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     614    2226    2368    3191    3399 
   Size:        614    1612     142     823     208

  Fragments arranged by size: 

                1612     823     614     208     142
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     450    3399 
   Size:        450    2949
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     334     373     805    1262    1373    1939    2335 
   Size:        328       6      39     432     457     111     566     396

Cuts at:   2335    2428    3399 
   Size:         93     971

  Fragments arranged by size: 

                 971     566     457     432     396     328     111      93
                  39       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     293    1975    2130    3136    3399 
   Size:        293    1682     155    1006     263

  Fragments arranged by size: 

                1682    1006     293     263     155
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     515    1033    1547    2148    3399 
   Size:        515     518     514     601    1251

  Fragments arranged by size: 

                1251     601     518     515     514
 
BsaHI Gr'CG_yC

Cuts at:      0     377    2939    3399 
   Size:        377    2562     460

  Fragments arranged by size: 

                2562     460     377
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     690     720    2895    2925    3399 
   Size:        690      30    2175      30     474

  Fragments arranged by size: 

                2175     690     474      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     638    2895    3399 
   Size:        638    2257     504

  Fragments arranged by size: 

                2257     638     504
 
BseYI C'CCAG_C

Cuts at:      0     413    1372    2762    2959    3051    3177    3286    3399 
   Size:        413     959    1390     197      92     126     109     113

  Fragments arranged by size: 

                1390     959     413     197     126     113     109      92
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1400    2030    2398    3338    3399 
   Size:       1400     630     368     940      61

  Fragments arranged by size: 

                1400     940     630     368      61
 
BsiEI CG_ry'CG

Cuts at:      0     197    3399 
   Size:        197    3202
 
BsiHKAI G_wGCw'C

Cuts at:      0     504    2611    2865    3399 
   Size:        504    2107     254     534

  Fragments arranged by size: 

                2107     534     504     254
 
BsmI GAATG_Cn'

Cuts at:      0    3249    3399 
   Size:       3249     150
 
Bsp1286I G_dGCh'C

Cuts at:      0     178     504    1373    1971    2123    2232    2611    2865 
   Size:        178     326     869     598     152     109     379     254

Cuts at:   2865    3034    3399 
   Size:        169     365

  Fragments arranged by size: 

                 869     598     379     365     326     254     178     169
                 152     109
 
BspHI T'CATG_A

Cuts at:      0    2003    2201    3399 
   Size:       2003     198    1198

  Fragments arranged by size: 

                2003    1198     198
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1676    2373    2700    3399 
   Size:       1676     697     327     699

  Fragments arranged by size: 

                1676     699     697     327
 
BsrBI CCG'CTC

Cuts at:      0     225    3240    3399 
   Size:        225    3015     159

  Fragments arranged by size: 

                3015     225     159
 
BsrFI r'CCGG_y

Cuts at:      0     151     379     385    1342    3399 
   Size:        151     228       6     957    2057

  Fragments arranged by size: 

                2057     957     228     151       6
 
BsrGI T'GTAC_A

Cuts at:      0    2597    3399 
   Size:       2597     802
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1237    2895    3075    3399 
   Size:       1237    1658     180     324

  Fragments arranged by size: 

                1658    1237     324     180
 
BstBI TT'CG_AA

Cuts at:      0     576    3399 
   Size:        576    2823
 
BstEII G'GTnAC_C

Cuts at:      0    3391    3399 
   Size:       3391       8
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     532    2515    3399 
   Size:        532    1983     884

  Fragments arranged by size: 

                1983     884     532
 
BstYI r'GATC_y

Cuts at:      0     723    1114    3399 
   Size:        723     391    2285

  Fragments arranged by size: 

                2285     723     391
 
Bsu36I CC'TnA_GG

Cuts at:      0     962    1548    3251    3399 
   Size:        962     586    1703     148

  Fragments arranged by size: 

                1703     962     586     148
 
BtgI C'CryG_G

Cuts at:      0     215    1109    1166    2033    2058    3059    3399 
   Size:        215     894      57     867      25    1001     340

  Fragments arranged by size: 

                1001     894     867     340     215      57      25
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3065    3399 
   Size:       3065     334
 
BtsI GCAGTG_nn'

Cuts at:      0     631     783    1337    3399 
   Size:        631     152     554    2062

  Fragments arranged by size: 

                2062     631     554     152
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3255    3290    3399 
   Size:       3255      35     109

  Fragments arranged by size: 

                3255     109      35
 
EaeI y'GGCC_r

Cuts at:      0     194     947    1169    1340    1847    2525    3399 
   Size:        194     753     222     171     507     678     874

  Fragments arranged by size: 

                 874     753     678     507     222     194     171
 
EagI C'GGCC_G

Cuts at:      0     194    3399 
   Size:        194    3205
 
EarI CTCTTCn'nnn_

Cuts at:      0     656     995    1066    2957    3399 
   Size:        656     339      71    1891     442

  Fragments arranged by size: 

                1891     656     442     339      71
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     220     454    2323    3399 
   Size:        220     234    1869    1076

  Fragments arranged by size: 

                1869    1076     234     220
 
EcoICRI GAG'CTC

Cuts at:      0     502    3399 
   Size:        502    2897
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     334     373     623     805    1262    1373    1939 
   Size:        328       6      39     250     182     457     111     566

Cuts at:   1939    2335    2428    3399 
   Size:        396      93     971

  Fragments arranged by size: 

                 971     566     457     396     328     250     182     111
                  93      39       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2805    3139    3399 
   Size:       2805     334     260

  Fragments arranged by size: 

                2805     334     260
 
EcoO109I rG'GnC_Cy

Cuts at:      0     174     175     978    1357    1552    1959    2101    2164 
   Size:        174       1     803     379     195     407     142      63

Cuts at:   2164    2500    2810    3041    3399 
   Size:        336     310     231     358

  Fragments arranged by size: 

                 803     407     379     358     336     310     231     195
                 174     142      63       1
 
EcoRI G'AATT_C

Cuts at:      0     572    1018    3399 
   Size:        572     446    2381

  Fragments arranged by size: 

                2381     572     446
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3003    3399 
   Size:       3003     396
 
FspI TGC'GCA

Cuts at:      0    2540    3399 
   Size:       2540     859
 
HaeII r_GCGC'y

Cuts at:      0     321     380    2942    3399 
   Size:        321      59    2562     457

  Fragments arranged by size: 

                2562     457     321      59
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     123     155    3399 
   Size:        123      32    3244

  Fragments arranged by size: 

                3244     123      32
 
HincII GTy'rAC

Cuts at:      0     267     768    3399 
   Size:        267     501    2631

  Fragments arranged by size: 

                2631     501     267
 
KasI G'GCGC_C

Cuts at:      0     376    2938    3399 
   Size:        376    2562     461

  Fragments arranged by size: 

                2562     461     376
 
KpnI G_GTAC'C

Cuts at:      0     285    3399 
   Size:        285    3114
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     473     583    2061    2525    2691    3399 
   Size:        473     110    1478     464     166     708

  Fragments arranged by size: 

                1478     708     473     464     166     110
 
MscI TGG'CCA

Cuts at:      0     949    1849    2527    3399 
   Size:        949     900     678     872

  Fragments arranged by size: 

                 949     900     872     678
 
MslI CAynn'nnrTG

Cuts at:      0     217    1714    1811    2614    2743    3399 
   Size:        217    1497      97     803     129     656

  Fragments arranged by size: 

                1497     803     656     217     129      97
 
MspA1I CmG'CkG

Cuts at:      0      22     124     306     345    2226    2361    2536    3055 
   Size:         22     102     182      39    1881     135     175     519

Cuts at:   3055    3399 
   Size:        344

  Fragments arranged by size: 

                1881     519     344     182     175     135     102      39
                  22
 
NaeI GCC'GGC

Cuts at:      0     381    3399 
   Size:        381    3018
 
NarI GG'CG_CC

Cuts at:      0     377    2939    3399 
   Size:        377    2562     460

  Fragments arranged by size: 

                2562     460     377
 
NcoI C'CATG_G

Cuts at:      0    1109    2033    3059    3399 
   Size:       1109     924    1026     340

  Fragments arranged by size: 

                1109    1026     924     340
 
NdeI CA'TA_TG

Cuts at:      0    1569    3399 
   Size:       1569    1830
 
NgoMIV G'CCGG_C

Cuts at:      0     379    3399 
   Size:        379    3020
 
NspI r_CATG'y

Cuts at:      0     630    1105    1386    2454    2742    2898    3159    3399 
   Size:        630     475     281    1068     288     156     261     240

  Fragments arranged by size: 

                1068     630     475     288     281     261     240     156
            
 
PasI CC'CwG_GG

Cuts at:      0    1964    2515    2676    3399 
   Size:       1964     551     161     723

  Fragments arranged by size: 

                1964     723     551     161
 
PciI A'CATG_T

Cuts at:      0    1101    1382    3399 
   Size:       1101     281    2017

  Fragments arranged by size: 

                2017    1101     281
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     517    3399 
   Size:        517    2882
 
PfoI T'CCnGG_A

Cuts at:      0    3308    3399 
   Size:       3308      91
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     690     722    3399 
   Size:        690      32    2677

  Fragments arranged by size: 

                2677     690      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     978    1552    2500    3041    3399 
   Size:        978     574     948     541     358

  Fragments arranged by size: 

                 978     948     574     541     358
 
PshAI GACnn'nnGTC

Cuts at:      0    1823    3399 
   Size:       1823    1576
 
PspOMI G'GGCC_C

Cuts at:      0     174    2228    3399 
   Size:        174    2054    1171

  Fragments arranged by size: 

                2054    1171     174
 
PstI C_TGCA'G

Cuts at:      0    1590    1673    2834    3399 
   Size:       1590      83    1161     565

  Fragments arranged by size: 

                1590    1161     565      83
 
PvuII CAG'CTG

Cuts at:      0     306    2536    3055    3399 
   Size:        306    2230     519     344

  Fragments arranged by size: 

                2230     519     344     306
 
SacI G_AGCT'C

Cuts at:      0     504    3399 
   Size:        504    2895
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1066    3399 
   Size:       1066    2333
 
ScaI AGT'ACT

Cuts at:      0     117    3399 
   Size:        117    3282
 
SfcI C'TryA_G

Cuts at:      0    1586    1643    1669    2830    3399 
   Size:       1586      57      26    1161     569

  Fragments arranged by size: 

                1586    1161     569      57      26
 
SfoI GGC'GCC

Cuts at:      0     378    2940    3399 
   Size:        378    2562     459

  Fragments arranged by size: 

                2562     459     378
 
SmaI CCC'GGG

Cuts at:      0    3089    3399 
   Size:       3089     310
 
SpeI A'CTAG_T

Cuts at:      0    1174    2908    3399 
   Size:       1174    1734     491

  Fragments arranged by size: 

                1734    1174     491
 
SphI G_CATG'C

Cuts at:      0    2454    2742    2898    3159    3399 
   Size:       2454     288     156     261     240

  Fragments arranged by size: 

                2454     288     261     240     156
 
SspI AAT'ATT

Cuts at:      0    1773    3399 
   Size:       1773    1626
 
StuI AGG'CCT

Cuts at:      0    1131    2800    3399 
   Size:       1131    1669     599

  Fragments arranged by size: 

                1669    1131     599
 
StyI C'CwwG_G

Cuts at:      0    1109    2033    2806    2824    3059    3399 
   Size:       1109     924     773      18     235     340

  Fragments arranged by size: 

                1109     924     773     340     235      18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2697    3191    3399 
   Size:       2697     494     208

  Fragments arranged by size: 

                2697     494     208
 
TatI w'GTAC_w

Cuts at:      0     115    2597    3399 
   Size:        115    2482     802

  Fragments arranged by size: 

                2482     802     115
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2895    2927    3399 
   Size:       2895      32     472

  Fragments arranged by size: 

                2895     472      32
 
XbaI T'CTAG_A

Cuts at:      0     717    3399 
   Size:        717    2682
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    3359    3399 
   Size:       3359      40
 
XmaI C'CCGG_G

Cuts at:      0    3087    3399 
   Size:       3087     312
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AleI     AlfI     AloI    AlwNI     ApaI
     ApoI     AseI     AvaI    AvrII     BanI    BanII     BbeI     BbsI
     BclI     BglI    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI    CspCI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NspI     PasI     PciI    PflMI     PfoI
     PpiI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI     SapI
     ScaI     SfcI     SfoI     SmaI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
    ApaLI     AscI    AsiSI     BaeI    BamHI    BbvCI     BcgI    BciVI
    BfrBI     BlpI     BmtI    BpuEI    BsaAI    BsaBI    BsaWI    BsiWI
    BsmBI    BspEI    BsrDI   BssHII    BssSI  BstZ17I     ClaI     DraI
   DraIII     DrdI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     MluI     NheI     NotI     NruI     NsiI     PacI     PmeI     PmlI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI    SexAI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SrfI
     SwaI  Tth111I     XhoI     XmnI     ZraI
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