Restriction map for pF1KA1408
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AgeI        Aor51HI     AscI        Asp718I     AspI        
AvaI        AviII       AvrII       BanI        BbeI        
BbrPI       BcgI        BciVI       BcnI        BglI        
BlnI        BsaAI       BsaHI       BsaI        BsgI        
BsiEI       BsiWI       BsoBI       BspDI       BspHI       
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstBI       BstUI       BstXI       BstZ17I     Cfr10I      
ClaI        CpoI        DrdI        DsaI        EagI        
EclXI       Eco47III    Eco52I      EcoNI       FauI        
FseI        FspI        HgaI        Hin1I       KasI        
KpnI        KspI        MfeI        MluI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SbfI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       Sse8387I    SwaI        Tth111I     
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI          935  GTCGAC               GT^[AC][GT]AC
AflII        3304  CTTAAG               C^TTAAG
AhdI          559  GACTCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         2163  CCTGAGG              CC^T[ACGT]AGG
ApaI         3457  GGGCCC               GGGCC^C
AspEI         559  GACTCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         2787  TGGCCA               TGG^CCA
BamHI        2525  GGATCC               G^GATCC
BclI         2924  TGATCA               T^GATCA
BfrI         3304  CTTAAG               C^TTAAG
BlpI            7  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I        7  GCTGAGC              GC^T[ACGT]AGC
BsaBI        2439  GATAAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        3810  GAGACG               CGTCTC(1/5)
Bsp1407I     2336  TGTACA               T^GTACA
BspMI        2994  GCAGGT               ACCTGC(4/8)
BsrGI        2336  TGTACA               T^GTACA
BstEII       2355  GGTTACC              G^GT[ACGT]ACC
BstPI        2355  GGTTACC              G^GT[ACGT]ACC
Bsu36I       2163  CCTGAGG              CC^T[ACGT]AGG
CelII           7  GCTGAGC              GC^T[ACGT]AGC
DraIII       2530  CACGTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         2787  TGGCCA               [CT]^GGCC[GA]
Eam1105I      559  GACTCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2163  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      2355  GGTTACC              G^GT[ACGT]ACC
EcoRI        3016  GAATTC               G^AATTC
EcoT14I       970  CCAAGG               C^C[AT][AT]GG
FbaI         2924  TGATCA               T^GATCA
HaeII        3710  AGCGCC               [GA]GCGC^[CT]
HindIII       398  AAGCTT               A^AGCTT
HpaI         3519  GTTAAC               GTT^AAC
MamI         2439  GATAAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        2787  TGGCCA               TGG^CCA
MscI         2787  TGGCCA               TGG^CCA
PflMI        2529  CCACGTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI          935  GTCGAC               G^TCGAC
SapI          316  GAAGAGC              GCTCTTC(1/4)
SexAI        1335  ACCTGGT              A^CC[AT]GGT
SpeI         2499  ACTAGT               A^CTAGT
SphI         2107  GCATGC               GCATG^C
SspBI        2336  TGTACA               T^GTACA
StuI         3033  AGGCCT               AGG^CCT
StyI          970  CCAAGG               C^C[AT][AT]GG
Tsp45I       2233  GTCAC                ^GT[GC]AC
Van91I       2529  CCACGTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1572  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccIII        453  TCCGGA               T^CCGGA
             2324  TCCGGA              
AflIII       2125  ACATGT               A^C[GA][CT]GT
             2782  ACATGT              
Alw44I        667  GTGCAC               G^TGCAC
             3403  GTGCAC              
AlwNI         608  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1219  CAGAAGCTG           
ApaLI         667  GTGCAC               G^TGCAC
             3403  GTGCAC              
AseI          883  ATTAAT               AT^TAAT
             1579  ATTAAT              
AsnI          883  ATTAAT               AT^TAAT
             1579  ATTAAT              
Asp700I       376  GAATCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2551  GAAGATATTC          
BbsI          160  GAAGAC               GAAGAC(2/6)
              202  GAAGAC              
BbvCI        1929  GCTGAGG              CCTCAGC(-5/-2)
             3447  GCTGAGG             
BpuAI         160  GAAGAC               GAAGAC(2/6)
              202  GAAGAC              
BsaWI         453  TCCGGA               [AT]^CCGG[AT]
             2324  TCCGGA              
BseAI         453  TCCGGA               T^CCGGA
             2324  TCCGGA              
BsmI         2247  GAATGC               GAATGC(1/-1)
             1148  GCATTC              
BspEI         453  TCCGGA               T^CCGGA
             2324  TCCGGA              
BspLU11I     2125  ACATGT               A^CATGT
             2782  ACATGT              
BstAPI       2205  GCAAGCAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3772  GCACCTAATGC         
EcoRV         118  GATATC               GAT^ATC
             3652  GATATC              
EcoT22I      2212  ATGCAT               ATGCA^T
             2249  ATGCAT              
HapII         454  CCGG                 C^CGG
             2325  CCGG                
HpaII         454  CCGG                 C^CGG
             2325  CCGG                
MroI          453  TCCGGA               T^CCGGA
             2324  TCCGGA              
MspI          454  CCGG                 C^CGG
             2325  CCGG                
NsiI         2212  ATGCAT               ATGCA^T
             2249  ATGCAT              
PshBI         883  ATTAAT               AT^TAAT
             1579  ATTAAT              
SacI         1117  GAGCTC               GAGCT^C
             1168  GAGCTC              
ScaI         1073  AGTACT               AGT^ACT
             2792  AGTACT              
SspI          877  AATATT               AAT^ATT
             1975  AATATT              
XmnI          376  GAATCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2551  GAAGATATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BanII       BbvI        
BfaI        BglII       BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
EarI        EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HhaI        HinP1I      HincII      HindII      
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       
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