Restriction map for pF1KA1408
 (Linear) (Six-base) MAPSORT of: KIAA1408.seq  Check: 6144  from: 1  to: 3816



KIAA1408.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:55  ..

AccI GT'mk_AC

Cuts at:      0     936    3816 
   Size:        936    2880
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     179     465     706    1640    3816 
   Size:        179     286     241     934    2176

  Fragments arranged by size: 

                2176     934     286     241     179
 
AflII C'TTAA_G

Cuts at:      0    3304    3816 
   Size:       3304     512
 
AflIII A'CryG_T

Cuts at:      0    2125    2782    3816 
   Size:       2125     657    1034

  Fragments arranged by size: 

                2125    1034     657
 
AhdI GACnn_n'nnGTC

Cuts at:      0     564    3816 
   Size:        564    3252
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2237    3719    3816 
   Size:       2237    1482      97

  Fragments arranged by size: 

                2237    1482      97
 
AlwNI CAG_nnn'CTG

Cuts at:      0     613    1224    3816 
   Size:        613     611    2592

  Fragments arranged by size: 

                2592     613     611
 
ApaI G_GGCC'C

Cuts at:      0    3461    3816 
   Size:       3461     355
 
ApaLI G'TGCA_C

Cuts at:      0     667    3403    3816 
   Size:        667    2736     413

  Fragments arranged by size: 

                2736     667     413
 
ApoI r'AATT_y

Cuts at:      0     588    1201    1233    1395    2587    3016    3816 
   Size:        588     613      32     162    1192     429     800

  Fragments arranged by size: 

                1192     800     613     588     429     162      32
 
AseI AT'TA_AT

Cuts at:      0     884    1580    3816 
   Size:        884     696    2236

  Fragments arranged by size: 

                2236     884     696
 
BamHI G'GATC_C

Cuts at:      0    2525    3816 
   Size:       2525    1291
 
BanII G_rGCy'C

Cuts at:      0    1121    1172    3461    3816 
   Size:       1121      51    2289     355

  Fragments arranged by size: 

                2289    1121     355      51
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     167     209    3816 
   Size:        167      42    3607

  Fragments arranged by size: 

                3607     167      42
 
BbvCI CC'TCA_GC

Cuts at:      0    1930    3448    3816 
   Size:       1930    1518     368

  Fragments arranged by size: 

                1930    1518     368
 
BclI T'GATC_A

Cuts at:      0    2924    3816 
   Size:       2924     892
 
BfrBI ATG'CAT

Cuts at:      0    2214    2251    3816 
   Size:       2214      37    1565

  Fragments arranged by size: 

                2214    1565      37
 
BglII A'GATC_T

Cuts at:      0    1275    1623    1743    2146    3783    3816 
   Size:       1275     348     120     403    1637      33

  Fragments arranged by size: 

                1637    1275     403     348     120      33
 
BlpI GC'TnA_GC

Cuts at:      0       8    3816 
   Size:          8    3808
 
Bme1580I G_kGCm'C

Cuts at:      0     671    1821    3407    3461    3816 
   Size:        671    1150    1586      54     355

  Fragments arranged by size: 

                1586    1150     671     355      54
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    3475    3816 
   Size:       3475     341
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1031    3764    3816 
   Size:       1031    2733      52

  Fragments arranged by size: 

                2733    1031      52
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1105    1367    2135    2140    2258    2296    3704    3816 
   Size:       1105     262     768       5     118      38    1408     112

  Fragments arranged by size: 

                1408    1105     768     262     118     112      38       5
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1245    1930    2738    3448    3816 
   Size:       1245     685     808     710     368

  Fragments arranged by size: 

                1245     808     710     685     368
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     480    1268    1579    1675    3816 
   Size:        480     788     311      96    2141

  Fragments arranged by size: 

                2141     788     480     311      96
 
BsaBI GATnn'nnATC

Cuts at:      0    2443    3816 
   Size:       2443    1373
 
BsaWI w'CCGG_w

Cuts at:      0     453    2324    3816 
   Size:        453    1871    1492

  Fragments arranged by size: 

                1871    1492     453
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3798    3816 
   Size:       3798      18
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     772    2297    3187    3226    3816 
   Size:        772    1525     890      39     590

  Fragments arranged by size: 

                1525     890     772     590      39
 
BseYI C'CCAG_C

Cuts at:      0    1434    3714    3816 
   Size:       1434    2280     102

  Fragments arranged by size: 

                2280    1434     102
 
BsiHKAI G_wGCw'C

Cuts at:      0     671    1121    1172    2507    3407    3774    3816 
   Size:        671     450      51    1335     900     367      42

  Fragments arranged by size: 

                1335     900     671     450     367      51      42
 
BsmI GAATG_Cn'

Cuts at:      0    1148    2253    3816 
   Size:       1148    1105    1563

  Fragments arranged by size: 

                1563    1148    1105
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3804    3816 
   Size:       3804      12
 
Bsp1286I G_dGCh'C

Cuts at:      0     671    1121    1172    1821    2507    3407    3461    3774 
   Size:        671     450      51     649     686     900      54     313

Cuts at:   3774    3816 
   Size:         42

  Fragments arranged by size: 

                 900     686     671     649     450     313      54      51
                  42
 
BspEI T'CCGG_A

Cuts at:      0     453    2324    3816 
   Size:        453    1871    1492

  Fragments arranged by size: 

                1871    1492     453
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2985    3816 
   Size:       2985     831
 
BsrDI GCAATG_nn'

Cuts at:      0     602    1769    1882    2216    3816 
   Size:        602    1167     113     334    1600

  Fragments arranged by size: 

                1600    1167     602     334     113
 
BsrGI T'GTAC_A

Cuts at:      0    2336    3816 
   Size:       2336    1480
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2211    3778    3816 
   Size:       2211    1567      38

  Fragments arranged by size: 

                2211    1567      38
 
BstEII G'GTnAC_C

Cuts at:      0    2355    3816 
   Size:       2355    1461
 
BstYI r'GATC_y

Cuts at:      0    1275    1623    1681    1743    1914    2146    2343    2525 
   Size:       1275     348      58      62     171     232     197     182

Cuts at:   2525    3551    3783    3816 
   Size:       1026     232      33

  Fragments arranged by size: 

                1275    1026     348     232     232     197     182     171
                  62      58      33
 
Bsu36I CC'TnA_GG

Cuts at:      0    2164    3816 
   Size:       2164    1652
 
BtsI GCAGTG_nn'

Cuts at:      0      81     128    2050    2616    3816 
   Size:         81      47    1922     566    1200

  Fragments arranged by size: 

                1922    1200     566      81      47
 
DraI TTT'AAA

Cuts at:      0    1067    1449    2063    3816 
   Size:       1067     382     614    1753

  Fragments arranged by size: 

                1753    1067     614     382
 
DraIII CAC_nnn'GTG

Cuts at:      0    2535    3816 
   Size:       2535    1281
 
EaeI y'GGCC_r

Cuts at:      0    2787    3816 
   Size:       2787    1029
 
EarI CTCTTCn'nnn_

Cuts at:      0     166     263     293     311    1828    3083    3164    3203 
   Size:        166      97      30      18    1517    1255      81      39

Cuts at:   3203    3212    3263    3614    3816 
   Size:          9      51     351     202

  Fragments arranged by size: 

                1517    1255     351     202     166      97      81      51
                  39      30      18       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      99    3816 
   Size:         99    3717
 
EcoICRI GAG'CTC

Cuts at:      0    1119    1170    3816 
   Size:       1119      51    2646

  Fragments arranged by size: 

                2646    1119      51
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     179     465     706    1105    1367    1640    2135    2140 
   Size:        179     286     241     399     262     273     495       5

Cuts at:   2140    2258    2296    3704    3816 
   Size:        118      38    1408     112

  Fragments arranged by size: 

                1408     495     399     286     273     262     241     179
                 118     112      38       5
 
EcoO109I rG'GnC_Cy

Cuts at:      0     153    3457    3458    3816 
   Size:        153    3304       1     358

  Fragments arranged by size: 

                3304     358     153       1
 
EcoRI G'AATT_C

Cuts at:      0    3016    3816 
   Size:       3016     800
 
EcoRV GAT'ATC

Cuts at:      0     120    3654    3816 
   Size:        120    3534     162

  Fragments arranged by size: 

                3534     162     120
 
HaeII r_GCGC'y

Cuts at:      0    3714    3816 
   Size:       3714     102
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     238     270     550     582     883     915    1017    1049 
   Size:        238      32     280      32     301      32     102      32

Cuts at:   1049    1673    1705    2489    2521    2942    2974    3037    3069 
   Size:        624      32     784      32     421      32      63      32

Cuts at:   3069    3475    3507    3816 
   Size:        406      32     309

  Fragments arranged by size: 

                 784     624     421     406     309     301     280     238
                 102      63      32      32      32      32      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     937    1737    3521    3816 
   Size:        937     800    1784     295

  Fragments arranged by size: 

                1784     937     800     295
 
HindIII A'AGCT_T

Cuts at:      0     398    3816 
   Size:        398    3418
 
HpaI GTT'AAC

Cuts at:      0    3521    3816 
   Size:       3521     295
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1508    1544    1826    3816 
   Size:       1508      36     282    1990

  Fragments arranged by size: 

                1990    1508     282      36
 
MscI TGG'CCA

Cuts at:      0    2789    3816 
   Size:       2789    1027
 
MslI CAynn'nnrTG

Cuts at:      0     908    1780    2219    3816 
   Size:        908     872     439    1597

  Fragments arranged by size: 

                1597     908     872     439
 
MspA1I CmG'CkG

Cuts at:      0       7     352    2278    2659    3447    3816 
   Size:          7     345    1926     381     788     369

  Fragments arranged by size: 

                1926     788     381     369     345       7
 
NsiI A_TGCA'T

Cuts at:      0    2216    2253    3816 
   Size:       2216      37    1563

  Fragments arranged by size: 

                2216    1563      37
 
NspI r_CATG'y

Cuts at:      0    1899    2111    2129    2786    3313    3816 
   Size:       1899     212      18     657     527     503

  Fragments arranged by size: 

                1899     657     527     503     212      18
 
PciI A'CATG_T

Cuts at:      0    2125    2782    3816 
   Size:       2125     657    1034

  Fragments arranged by size: 

                2125    1034     657
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2535    3816 
   Size:       2535    1281
 
PfoI T'CCnGG_A

Cuts at:      0    2312    3816 
   Size:       2312    1504
 
PspOMI G'GGCC_C

Cuts at:      0    3457    3816 
   Size:       3457     359
 
PstI C_TGCA'G

Cuts at:      0      76     993    3498    3816 
   Size:         76     917    2505     318

  Fragments arranged by size: 

                2505     917     318      76
 
PvuII CAG'CTG

Cuts at:      0       7     352    2278    2659    3447    3816 
   Size:          7     345    1926     381     788     369

  Fragments arranged by size: 

                1926     788     381     369     345       7
 
SacI G_AGCT'C

Cuts at:      0    1121    1172    3816 
   Size:       1121      51    2644

  Fragments arranged by size: 

                2644    1121      51
 
SalI G'TCGA_C

Cuts at:      0     935    3816 
   Size:        935    2881
 
SapI GCTCTTCn'nnn_

Cuts at:      0     311    3816 
   Size:        311    3505
 
ScaI AGT'ACT

Cuts at:      0    1075    2794    3816 
   Size:       1075    1719    1022

  Fragments arranged by size: 

                1719    1075    1022
 
SexAI A'CCwGG_T

Cuts at:      0    1335    3816 
   Size:       1335    2481
 
SfcI C'TryA_G

Cuts at:      0      72     614     917     989    1714    3373    3494    3816 
   Size:         72     542     303      72     725    1659     121     322

  Fragments arranged by size: 

                1659     725     542     322     303     121      72      72
            
 
SmlI C'TyrA_G

Cuts at:      0     495    1283    1558    1690    3304    3816 
   Size:        495     788     275     132    1614     512

  Fragments arranged by size: 

                1614     788     512     495     275     132
 
SpeI A'CTAG_T

Cuts at:      0    2499    3816 
   Size:       2499    1317
 
SphI G_CATG'C

Cuts at:      0    2111    3816 
   Size:       2111    1705
 
SspI AAT'ATT

Cuts at:      0     879    1977    3816 
   Size:        879    1098    1839

  Fragments arranged by size: 

                1839    1098     879
 
StuI AGG'CCT

Cuts at:      0    3035    3816 
   Size:       3035     781
 
StyI C'CwwG_G

Cuts at:      0     970    3816 
   Size:        970    2846
 
TatI w'GTAC_w

Cuts at:      0    1073    2175    2336    2792    3816 
   Size:       1073    1102     161     456    1024

  Fragments arranged by size: 

                1102    1073    1024     456     161
 
XbaI T'CTAG_A

Cuts at:      0    1572    3816 
   Size:       1572    2244
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     577    2178    2518    2536    3816 
   Size:        577    1601     340      18    1280

  Fragments arranged by size: 

                1601    1280     577     340      18
 
XmnI GAAnn'nnTTC

Cuts at:      0     380    2555    3816 
   Size:        380    2175    1261

  Fragments arranged by size: 

                2175    1261     380
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AhdI     AlfI    AlwNI     ApaI
    ApaLI     ApoI     AseI    BamHI    BanII     BbsI    BbvCI     BclI
    BfrBI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI    BsaBI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspMI    BsrDI    BsrGI   BstAPI   BstEII
    BstYI   Bsu36I     BtsI     DraI   DraIII     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV    HaeII    Hin4I   HincII
  HindIII     HpaI     MmeI     MscI     MslI   MspA1I     NsiI     NspI
     PciI    PflMI     PfoI   PspOMI     PstI    PvuII     SacI     SalI
     SapI     ScaI    SexAI     SfcI     SmlI     SpeI     SphI     SspI
     StuI     StyI     TatI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AleI     AloI
     AscI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI     BcgI
    BciVI     BglI    BmgBI     BmtI     BsaI    BsaAI    BsaHI     BsgI
    BsiEI    BsiWI    BspHI    BsrBI    BsrFI   BssHII    BssSI    BstBI
    BstXI  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DrdI     EagI
    EcoNI     FalI     FseI     FspI    FspAI     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII    SanDI     SbfI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SrfI     SwaI
    TaqII     TstI  Tth111I     XhoI     XmaI     ZraI
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