Restriction map for pF1KA0972
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

Acc65I      AccII       AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaI        BsgI        BsiEI       BsiHKAI     
BsiWI       BsoBI       Bsp1286I    BspDI       BspLU11I    
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     CelII       CfoI        
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoO65I     
EcoRV       EcoT22I     FauI        FseI        FspI        
HaeII       HgaI        HhaI        HinP1I      HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MflI        MluI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        TaqI        Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII        1161  GACGTC               GACGT^C
AccI         2001  GTCTAC               GT^[AC][GT]AC
AccIII       1829  TCCGGA               T^CCGGA
AciI          608  GCGG                 CCGC(-3/-1)
AcyI         1161  GACGTC               G[GA]^CG[CT]C
AlwI         1057  GATCC                GGATC(4/5)
AocI         1422  CCTCAGG              CC^T[ACGT]AGG
AseI          571  ATTAAT               AT^TAAT
AsnI          571  ATTAAT               AT^TAAT
BcnI         1745  CCCGG                CC^[GC]GG
BsaHI        1161  GACGTC               G[GA]^CG[CT]C
BsaWI        1829  TCCGGA               [AT]^CCGG[AT]
BseAI        1829  TCCGGA               T^CCGGA
BsiYI        1158  CCAGACGTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI         1158  CCAGACGTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         247  GTCTC                GTCTC(1/5)
BsmBI         246  CGTCTC               CGTCTC(1/5)
BsmFI        1428  GGGAC                GGGAC(10/14)
BsmI         1969  GAATGC               GAATGC(1/-1)
Bsp1407I      209  TGTACA               T^GTACA
BspEI        1829  TCCGGA               T^CCGGA
BspHI        1137  TCATGA               T^CATGA
BspMI         923  ACCTGC               ACCTGC(4/8)
BsrDI        1973  GCAATG               GCAATG(2/0)
BsrGI         209  TGTACA               T^GTACA
BstNI         168  CCAGG                CC^[AT]GG
Bsu36I       1422  CCTCAGG              CC^T[ACGT]AGG
DraI         1798  TTTAAA               TTT^AAA
Eco81I       1422  CCTCAGG              CC^T[ACGT]AGG
EcoRII        168  CCAGG                ^CC[AT]GG
EcoT14I       310  CCTTGG               C^C[AT][AT]GG
Hin1I        1161  GACGTC               G[GA]^CG[CT]C
MroI         1829  TCCGGA               T^CCGGA
MspA1I       1067  CAGCTG               C[AC]G^C[GT]G
MvaI          168  CCAGG                CC^[AT]GG
MwoI          493  GCTGCTGTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI         1745  CCCGG                CC^[GC]GG
NspI          486  GCATGT               [GA]CATG^[CT]
PshBI         571  ATTAAT               AT^TAAT
PvuII        1067  CAGCTG               CAG^CTG
RcaI         1137  TCATGA               T^CATGA
ScaI         1342  AGTACT               AGT^ACT
SspBI         209  TGTACA               T^GTACA
StyI          310  CCTTGG               C^C[AT][AT]GG
SwaI         1797  ATTTAAAT             ATTT^AAAT
XcmI          894  CCACAGGAGAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

BalI         1575  TGGCCA               TGG^CCA
             1911  TGGCCA              
BclI          374  TGATCA               T^GATCA
             1848  TGATCA              
BpmI          226  CTGGAG               CTGGAG(16/14)
              662  CTGGAG              
BsaJI         167  CCCAGG               C^C[ACGT][ACGT]GG
              310  CCTTGG              
BssKI         168  CCAGG                ^CC[ACGT]GG
             1745  CCCGG               
Cac8I         414  GCAAGC               GC[ACGT]^[ACGT]GC
              753  GCAAGC              
Cfr13I         51  GGGCC                G^G[ACGT]CC
              189  GGCCC               
EaeI         1575  TGGCCA               [CT]^GGCC[GA]
             1911  TGGCCA              
EarI          454  GAAGAG               CTCTTC(1/4)
             1078  GAAGAG              
FbaI          374  TGATCA               T^GATCA
             1848  TGATCA              
HapII        1746  CCGG                 C^CGG
             1830  CCGG                
HincII        960  GTCAAC               GT[CT]^[GA]AC
             1416  GTCAAC              
HindII        960  GTCAAC               GT[CT]^[GA]AC
             1416  GTCAAC              
HindIII       755  AAGCTT               A^AGCTT
             2010  AAGCTT              
HpaII        1746  CCGG                 C^CGG
             1830  CCGG                
Ksp632I       454  GAAGAG               CTCTTC(1/4)
             1078  GAAGAG              
MaeII         149  ACGT                 A^CGT
             1162  ACGT                
MluNI        1575  TGGCCA               TGG^CCA
             1911  TGGCCA              
MscI         1575  TGGCCA               TGG^CCA
             1911  TGGCCA              
MspI         1746  CCGG                 C^CGG
             1830  CCGG                
NlaIV         189  GGCCCC               GG[ACGT]^[ACGT]CC
              306  GGAGCC              
PleI         1944  GACTC                GAGTC(4/5)
              342  GAGTC               
Sau96I         51  GGGCC                G^G[ACGT]CC
              189  GGCCC               
ScrFI         168  CCAGG                CC^[ACGT]GG
             1745  CCCGG               
TfiI          204  GAATC                G^A[AT]TC
             2040  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        Asp700I     
BbvI        BseRI       BsrI        BstF5I      DdeI        
DpnII       EcoRI       Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        MaeIII      MboI        
MboII       MnlI        MseI        MslI        NdeII       
NlaIII      RsaI        Sau3AI      SfaNI       SfcI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com