Restriction map for pF1KA0868
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AhdI        
ApaI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        BamHI       BbrPI       
BbvCI       BclI        BfrI        BglII       BlpI        
Bpu1102I    BsaAI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BsrGI       Bst1107I    BstXI       BstZ17I     
CelII       CpoI        DraI        EagI        Eam1105I    
EclXI       Eco52I      EcoRI       EcoRV       FbaI        
FseI        HpaI        KpnI        KspI        MfeI        
MluI        MunI        NheI        NotI        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SbfI        
SexAI       SfiI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       StuI        SwaI        
Tth111I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII         651  GACGTC               GACGT^C
AccIII       2881  TCCGGA               T^CCGGA
Alw44I       3828  GTGCAC               G^TGCAC
AocI         1343  CCTCAGG              CC^T[ACGT]AGG
ApaLI        3828  GTGCAC               G^TGCAC
AviII         476  TGCGCA               TGC^GCA
AvrII        1812  CCTAGG               C^CTAGG
BbeI         3177  GGCGCC               GGCGC^C
BcgI         1374  GCACGAGGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1750  GGATAC               GTATCC(6/5)
BlnI         1812  CCTAGG               C^CTAGG
BsaBI        3385  GATCATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        2881  TCCGGA               T^CCGGA
BspEI        2881  TCCGGA               T^CCGGA
BsrBI        2811  CCGCTC               CCGCTC(-3/-3)
BssHII         15  GCGCGC               G^CGCGC
BstAPI       3067  GCAACAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1445  TTCGAA               TT^CGAA
Bsu36I       1343  CCTCAGG              CC^T[ACGT]AGG
DraIII       2112  CACTTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI         3433  CTCTTC               CTCTTC(1/4)
Eco81I       1343  CCTCAGG              CC^T[ACGT]AGG
EcoT22I      1557  ATGCAT               ATGCA^T
FspI          476  TGCGCA               TGC^GCA
HindIII      3249  AAGCTT               A^AGCTT
KasI         3177  GGCGCC               G^GCGCC
Ksp632I      3433  CTCTTC               CTCTTC(1/4)
MamI         3385  GATCATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2881  TCCGGA               T^CCGGA
NaeI           19  GCCGGC               GCC^GGC
NarI         3177  GGCGCC               GG^CGCC
NcoI         2003  CCATGG               C^CATGG
NdeI         2984  CATATG               CA^TATG
NgoMIV         19  GCCGGC               G^CCGGC
NruI         1631  TCGCGA               TCG^CGA
NsiI         1557  ATGCAT               ATGCA^T
NspV         1445  TTCGAA               TT^CGAA
PflMI        2304  CCAAGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII          57  CAGCTG               CAG^CTG
SacI         2749  GAGCTC               GAGCT^C
SfoI         3177  GGCGCC               GGC^GCC
SfuI         1445  TTCGAA               TT^CGAA
SspI         3295  AATATT               AAT^ATT
Van91I       2304  CCAAGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI         3097  GTAGAC               GT^[AC][GT]AC
             3304  GTAGAC              
AflIII       1649  ACATGT               A^C[GA][CT]GT
             3854  ACATGT              
Aor51HI        33  AGCGCT               AGC^GCT
             2458  AGCGCT              
AvaI         2889  CTCGGG               C^[CT]CG[GA]G
             3281  CCCGAG              
BanII        1069  GAGCCC               G[GA]GC[CT]^C
             2749  GAGCTC              
BsoBI        2889  CTCGGG               C^[CT]CG[GA]G
             3281  CCCGAG              
BspDI        1333  ATCGAT               AT^CGAT
             1420  ATCGAT              
BspHI        1923  TCATGA               T^CATGA
             3932  TCATGA              
BspLU11I     1649  ACATGT               A^CATGT
             3854  ACATGT              
BsrFI          19  GCCGGC               [GA]^CCGG[CT]
             2696  ACCGGC              
BssSI        1375  CACGAG               CACGAG(-5/-1)
             3358  CACGAG              
BstEII        327  GGTGACC              G^GT[ACGT]ACC
             2615  GGTCACC             
BstPI         327  GGTGACC              G^GT[ACGT]ACC
             2615  GGTCACC             
Cfr10I         19  GCCGGC               [GA]^CCGG[CT]
             2696  ACCGGC              
ClaI         1333  ATCGAT               AT^CGAT
             1420  ATCGAT              
DrdI          427  GACGGTGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1911  GACCATAGTGTC        
DsaI         2003  CCATGG               C^C[GA][CT]GG
             2952  CCACGG              
Eco47III       33  AGCGCT               AGC^GCT
             2458  AGCGCT              
EcoNI        1389  CCTAGCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3135  CCTGGCACAGG         
EcoO65I       327  GGTGACC              G^GT[ACGT]ACC
             2615  GGTCACC             
PpuMI        1175  AGGACCT              [GA]G^G[AT]CC[CT]
             2358  AGGTCCT             
PstI           66  CTGCAG               CTGCA^G
             2657  CTGCAG              
RcaI         1923  TCATGA               T^CATGA
             3932  TCATGA              
SphI          884  GCATGC               GCATG^C
             1559  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaII       BalI        BanI        BbsI        BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BpuAI       BsaHI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BspMI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
RsaI        Sau3AI      Sau96I      ScaI        ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       
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