Restriction map for pF1KA0868
 (Circular) (Six-base) MAPSORT of: pF1KA0868.seq  Check: 3827  from: 1  to: 7106



pF1KA0868  7106 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1882    2408    1882 
   Size:        526    6580
 
AatII G_ACGT'C

Cuts at:    763     763 
   Size:       7106
 
AccI GT'mk_AC

Cuts at:   3206    3413    4144    3206 
   Size:        207     731    6168

  Fragments arranged by size: 

                6168     731     207
 
Acc65I G'GTAC_C

Cuts at:   4122    4122 
   Size:       7106
 
AclI AA'CG_TT

Cuts at:   6946    6946 
   Size:       7106
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    346    1326    1630    2677    3249    3255    4931    5363    5716 
   Size:        980     304    1047     572       6    1676     432     353

Cuts at:   5716    6378     346 
   Size:        662    1074

  Fragments arranged by size: 

                1676    1074    1047     980     662     572     432     353
                 304       6
 
AfeI AGC'GCT

Cuts at:    143    2568     143 
   Size:       2425    4681
 
AflIII A'CryG_T

Cuts at:   1757    3962    4458    6258    6539    1757 
   Size:       2205     496    1800     281    2324

  Fragments arranged by size: 

                2324    2205    1800     496     281
 
AleI CACnn'nnGTG

Cuts at:    898    2671     898 
   Size:       1773    5333
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    410     410 
   Size:       7106
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1548    1580    1548 
   Size:         32    7074
 
AlwNI CAG_nnn'CTG

Cuts at:    167     182    1287    1459    4411    5849     167 
   Size:         15    1105     172    2952    1438    1424

  Fragments arranged by size: 

                2952    1438    1424    1105     172      15
 
ApaLI G'TGCA_C

Cuts at:   3936    5944    3936 
   Size:       2008    5098
 
ApoI r'AATT_y

Cuts at:   1101    1194    1509    1701    4110    6459    6715    1101 
   Size:         93     315     192    2409    2349     256    1492

  Fragments arranged by size: 

                2409    2349    1492     315     256     192      93
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       7106
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7106
 
AvaI C'yCGr_G

Cuts at:   2997    3389    4126    2997 
   Size:        392     737    5977

  Fragments arranged by size: 

                5977     737     392
 
AvrII C'CTAG_G

Cuts at:   1920    1920 
   Size:       7106
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    990    1023    3048    3081     990 
   Size:         33    2025      33    5015

  Fragments arranged by size: 

                5015    2025      33      33
 
BamHI G'GATC_C

Cuts at:   4131    4131 
   Size:       7106
 
BanI G'GyrC_C

Cuts at:   1227    1263    2753    3168    3285    3385    3800    3979    4122 
   Size:         36    1490     415     117     100     415     179     143

Cuts at:   4122    4536    4784    4819    1227 
   Size:        414     248      35    3514

  Fragments arranged by size: 

                3514    1490     415     415     414     248     179     143
                 117     100      36      35
 
BanII G_rGCy'C

Cuts at:   1181    2861    4120    5150    1181 
   Size:       1680    1259    1030    3137

  Fragments arranged by size: 

                3137    1680    1259    1030
 
BbeI G_GCGC'C

Cuts at:   3289    4540    4788    3289 
   Size:       1251     248    5607

  Fragments arranged by size: 

                5607    1251     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1332    3478    3939    1332 
   Size:       2146     461    4499

  Fragments arranged by size: 

                4499    2146     461
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124    1471    1505      90 
   Size:         34    1347      34    5691

  Fragments arranged by size: 

                5691    1347      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   1852    5004    6060    7106    1852 
   Size:       3152    1056    1046    1852

  Fragments arranged by size: 

                3152    1852    1056    1046
 
BclI T'GATC_A

Cuts at:   4626    4626 
   Size:       7106
 
BfrBI ATG'CAT

Cuts at:   1667    1667 
   Size:       7106
 
BglI GCCn_nnn'nGGC

Cuts at:    112     187    1272    2900    3977     112 
   Size:         75    1085    1628    1077    3241

  Fragments arranged by size: 

                3241    1628    1085    1077      75
 
BglII A'GATC_T

Cuts at:   4621    6658    4621 
   Size:       2037    5069
 
BlpI GC'TnA_GC

Cuts at:   4218    4218 
   Size:       7106
 
Bme1580I G_kGCm'C

Cuts at:   1268    1787    3940    3982    4731    4824    5948    1268 
   Size:        519    2153      42     749      93    1124    2426

  Fragments arranged by size: 

                2426    2153    1124     749     519      93      42
 
BmgBI CAC'GTC

Cuts at:    200    3714     200 
   Size:       3514    3592
 
BmrI ACTGGGnnnn_n'

Cuts at:    666    2268    4318    4399    4734    6878     666 
   Size:       1602    2050      81     335    2144     894

  Fragments arranged by size: 

                2144    2050    1602     894     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5473    5473 
   Size:       7106
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1071    1279    2671    2875    2974    3177    3630    1071 
   Size:        208    1392     204      99     203     453    4547

  Fragments arranged by size: 

                4547    1392     453     208     204     203      99
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1614    2946    3469    3661    3708    4282    5161    5875    6173 
   Size:       1332     523     192      47     574     879     714     298

Cuts at:   6173    1614 
   Size:       2547

  Fragments arranged by size: 

                2547    1332     879     714     574     523     298     192
                  47
 
BsaI GGTCTCn'nnnn_

Cuts at:    335    1336    4060    6810     335 
   Size:       1001    2724    2750     631

  Fragments arranged by size: 

                2750    2724    1001     631
 
BsaAI yAC'GTr

Cuts at:   4459    4459 
   Size:       7106
 
BsaBI GATnn'nnATC

Cuts at:      6    3497    4620    6657       6 
   Size:       3491    1123    2037     455

  Fragments arranged by size: 

                3491    2037    1123     455
 
BsaHI Gr'CG_yC

Cuts at:    760    1139    2050    3286    3905    4031    4537    4785    5487 
   Size:        379     911    1236     619     126     506     248     702

Cuts at:   5487    6983     760 
   Size:       1496     883

  Fragments arranged by size: 

                1496    1236     911     883     702     619     506     379
                 248     126
 
BsaWI w'CCGG_w

Cuts at:    446    2989    3956    4295    4816    5494    5905    6052    6326 
   Size:       2543     967     339     521     678     411     147     274

Cuts at:   6326     446 
   Size:       1226

  Fragments arranged by size: 

                2543    1226     967     678     521     411     339     274
                 147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   2092    2122    2512    2542    2644    2674    3052    3082    3295 
   Size:         30     390      30     102      30     378      30     213

Cuts at:   3295    3325    2092 
   Size:         30    5873

  Fragments arranged by size: 

                5873     390     378     213     102      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    249     252     326    3268    3289     249 
   Size:          3      74    2942      21    4066

  Fragments arranged by size: 

                4066    2942      74      21       3
 
BseYI C'CCAG_C

Cuts at:    319    1081    2887    4531    5289    5954    6362     319 
   Size:        762    1806    1644     758     665     408    1063

  Fragments arranged by size: 

                1806    1644    1063     762     758     665     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    769    2298    2521     769 
   Size:       1529     223    5354

  Fragments arranged by size: 

                5354    1529     223
 
BsiEI CG_ry'CG

Cuts at:    104    4648    4694    5924     104 
   Size:       4544      46    1230    1286

  Fragments arranged by size: 

                4544    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   1816    2861    3940    4120    4898    5088    5948    1816 
   Size:       1045    1079     180     778     190     860    2974

  Fragments arranged by size: 

                2974    1079    1045     860     778     190     180
 
BsmI GAATG_Cn'

Cuts at:    936    2508    3293     936 
   Size:       1572     785    4749

  Fragments arranged by size: 

                4749    1572     785
 
Bsp1286I G_dGCh'C

Cuts at:   1181    1268    1787    1816    2861    3940    3982    4120    4731 
   Size:         87     519      29    1045    1079      42     138     611

Cuts at:   4731    4824    4898    5088    5150    5948    1181 
   Size:         93      74     190      62     798    2339

  Fragments arranged by size: 

                2339    1079    1045     798     611     519     190     138
                  93      87      74      62      42      29
 
BspEI T'CCGG_A

Cuts at:   2989    2989 
   Size:       7106
 
BspHI T'CATG_A

Cuts at:   2031    4040    5538    2031 
   Size:       2009    1498    3599

  Fragments arranged by size: 

                3599    2009    1498
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1882    2408    2543    4156    4370    4672    5053    1882 
   Size:        526     135    1613     214     302     381    3935

  Fragments arranged by size: 

                3935    1613     526     381     302     214     135
 
BsrBI CCG'CTC

Cuts at:     50    2921    5398    5452    6936    7101      50 
   Size:       2871    2477      54    1484     165      55

  Fragments arranged by size: 

                2871    2477    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    398     740    1374    2365    3003    3908    5018     398 
   Size:        342     634     991     638     905    1110    2486

  Fragments arranged by size: 

                2486    1110     991     905     638     634     342
 
BsrFI r'CCGG_y

Cuts at:    127    2804    5104    5285     127 
   Size:       2677    2300     181    1948

  Fragments arranged by size: 

                2677    2300    1948     181
 
BssHII G'CGCG_C

Cuts at:    123     123 
   Size:       7106
 
BssSI C'ACGA_G

Cuts at:   1483    3466    5377    6085    1483 
   Size:       1983    1911     708    2504

  Fragments arranged by size: 

                2504    1983    1911     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3181    4483    3181 
   Size:       1302    5804
 
BstBI TT'CG_AA

Cuts at:   1554    5467    1554 
   Size:       3913    3193
 
BstEII G'GTnAC_C

Cuts at:    435    2723     435 
   Size:       2288    4818
 
BstYI r'GATC_y

Cuts at:      1    1950    2319    2819    3257    4131    4598    4621    4956 
   Size:       1949     369     500     438     874     467      23     335

Cuts at:   4956    5202    5508    5520    5606    6658       1 
   Size:        246     306      12      86    1052     449

  Fragments arranged by size: 

                1949    1052     874     500     467     449     438     369
                 335     306     246      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1452    1452 
   Size:       7106
 
BtgI C'CryG_G

Cuts at:   2111    3060    2111 
   Size:        949    6157
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   5056    5237    5400    5056 
   Size:        181     163    6762

  Fragments arranged by size: 

                6762     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2762    4349    2762 
   Size:       1587    5519
 
ClaI AT'CG_AT

Cuts at:   1442    1529    1442 
   Size:         87    7019
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   3363    3398    3363 
   Size:         35    7071
 
DraI TTT'AAA

Cuts at:   4105    4105 
   Size:       7106
 
DraIII CAC_nnn'GTG

Cuts at:   2225    2225 
   Size:       7106
 
DrdI GACnn_nn'nnGTC

Cuts at:    541    2025    4812    6156     541 
   Size:       1484    2787    1344    1491

  Fragments arranged by size: 

                2787    1491    1484    1344
 
EaeI y'GGCC_r

Cuts at:    681     888    3428    4691    4865    5256    5283    6628     681 
   Size:        207    2540    1263     174     391      27    1345    1159

  Fragments arranged by size: 

                2540    1345    1263    1159     391     207     174      27
            
 
EagI C'GGCC_G

Cuts at:   4691    4691 
   Size:       7106
 
EarI CTCTTCn'nnn_

Cuts at:   3547    5129    5339    3547 
   Size:       1582     210    5314

  Fragments arranged by size: 

                5314    1582     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    978    3774    3958    4024    5314    6056    6202    6916     978 
   Size:       2796     184      66    1290     742     146     714    1168

  Fragments arranged by size: 

                2796    1290    1168     742     714     184     146      66
            
 
EcoICRI GAG'CTC

Cuts at:   2859    4118    2859 
   Size:       1259    5847
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    346    1071    1279    1326    1630    2671    2677    2875    2974 
   Size:        725     208      47     304    1041       6     198      99

Cuts at:   2974    3177    3249    3255    3630    4931    5363    5716    6378 
   Size:        203      72       6     375    1301     432     353     662

Cuts at:   6378     346 
   Size:       1074

  Fragments arranged by size: 

                1301    1074    1041     725     662     432     375     353
                 304     208     203     198      99      72      47       6
                   6
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1501    3247    1501 
   Size:       1746    5360
 
EcoO109I rG'GnC_Cy

Cuts at:   1284    1975    2467    2829    3752    4245    1284 
   Size:        691     492     362     923     493    4145

  Fragments arranged by size: 

                4145     923     691     493     492     362
 
EcoRI G'AATT_C

Cuts at:   4110    4110 
   Size:       7106
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3495    3579    3702    5125    3495 
   Size:         84     123    1423    5476

  Fragments arranged by size: 

                5476    1423     123      84
 
FspI TGC'GCA

Cuts at:    586    4887     586 
   Size:       4301    2805
 
FspAI rTGC'GCAy

Cuts at:    586     586 
   Size:       7106
 
HaeII r_GCGC'y

Cuts at:    145     957    2108    2570    3289    4540    4788    6018    6789 
   Size:        812    1151     462     719    1251     248    1230     771

Cuts at:   6789     145 
   Size:        462

  Fragments arranged by size: 

                1251    1230    1151     812     771     719     462     462
                 248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1276    1308    1435    1467    1528    1560    2451    2483    2644 
   Size:         32     127      32      61      32     891      32     161

Cuts at:   2644    2676    2954    2986    3501    3533    3657    3689    4948 
   Size:         32     278      32     515      32     124      32    1259

Cuts at:   4948    4978    4980    5010    1276 
   Size:         30       2      30    3372

  Fragments arranged by size: 

                3372    1259     891     515     278     161     127     124
                  61      32      32      32      32      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:    369    1143    3453    3879    4145     369 
   Size:        774    2310     426     266    3330

  Fragments arranged by size: 

                3330    2310     774     426     266
 
HindIII A'AGCT_T

Cuts at:   3357    3357 
   Size:       7106
 
KasI G'GCGC_C

Cuts at:   3285    4536    4784    3285 
   Size:       1251     248    5607

  Fragments arranged by size: 

                5607    1251     248
 
KpnI G_GTAC'C

Cuts at:   4126    4126 
   Size:       7106
 
MluI A'CGCG_T

Cuts at:   6539    6539 
   Size:       7106
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2403    3557    3767    5865    6049    2403 
   Size:       1154     210    2098     184    3460

  Fragments arranged by size: 

                3460    2098    1154     210     184
 
MscI TGG'CCA

Cuts at:    683     890    3430    4867     683 
   Size:        207    2540    1437    2922

  Fragments arranged by size: 

                2922    2540    1437     207
 
MslI CAynn'nnrTG

Cuts at:    494     898    1681    1756    2671    3913    5095    5222     494 
   Size:        404     783      75     915    1242    1182     127    2378

  Fragments arranged by size: 

                2378    1242    1182     915     783     404     127      75
            
 
MspA1I CmG'CkG

Cuts at:    167    1964    3762    4217    4531    4891    5675    5920     167 
   Size:       1797    1798     455     314     360     784     245    1353

  Fragments arranged by size: 

                1798    1797    1353     784     455     360     314     245
            
 
NaeI GCC'GGC

Cuts at:    129    5287     129 
   Size:       5158    1948
 
NarI GG'CG_CC

Cuts at:   3286    4537    4785    3286 
   Size:       1251     248    5607

  Fragments arranged by size: 

                5607    1251     248
 
NcoI C'CATG_G

Cuts at:   2111    2111 
   Size:       7106
 
NdeI CA'TA_TG

Cuts at:   3093    3093 
   Size:       7106
 
NgoMIV G'CCGG_C

Cuts at:    127    5285     127 
   Size:       5158    1948
 
NruI TCG'CGA

Cuts at:   1741    1741 
   Size:       7106
 
NsiI A_TGCA'T

Cuts at:   1669    1669 
   Size:       7106
 
NspI r_CATG'y

Cuts at:    996    1671    1761    3966    4159    4659    6262     996 
   Size:        675      90    2205     193     500    1603    1840

  Fragments arranged by size: 

                2205    1840    1603     675     500     193      90
 
PasI CC'CwG_GG

Cuts at:   2603    2603 
   Size:       7106
 
PciI A'CATG_T

Cuts at:   1757    3962    6258    1757 
   Size:       2205    2296    2605

  Fragments arranged by size: 

                2605    2296    2205
 
PflMI CCAn_nnn'nTGG

Cuts at:   2418    2418 
   Size:       7106
 
PfoI T'CCnGG_A

Cuts at:    516    3846     516 
   Size:       3330    3776
 
PmeI GTTT'AAAC

Cuts at:   4105    4105 
   Size:       7106
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5552    5584    5552 
   Size:         32    7074
 
PpuMI rG'GwC_Cy

Cuts at:   1284    2467    1284 
   Size:       1183    5923
 
PstI C_TGCA'G

Cuts at:    178    2769    4153    4838     178 
   Size:       2591    1384     685    2446

  Fragments arranged by size: 

                2591    2446    1384     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       7106
 
PvuII CAG'CTG

Cuts at:    167    4531    4891     167 
   Size:       4364     360    2382

  Fragments arranged by size: 

                4364    2382     360
 
RsrII CG'GwC_CG

Cuts at:   5301    5301 
   Size:       7106
 
SacI G_AGCT'C

Cuts at:   2861    4120    2861 
   Size:       1259    5847
 
SalI G'TCGA_C

Cuts at:   4143    4143 
   Size:       7106
 
SapI GCTCTTCn'nnn_

Cuts at:   5129    5339    5129 
   Size:        210    6896
 
SbfI CC_TGCA'GG

Cuts at:   4153    4153 
   Size:       7106
 
ScaI AGT'ACT

Cuts at:   1594    2149    2329    6623    1594 
   Size:        555     180    4294    2077

  Fragments arranged by size: 

                4294    2077     555     180
 
SfcI C'TryA_G

Cuts at:     33     174     456    2765    2794    2862    4149    4834    5802 
   Size:        141     282    2309      29      68    1287     685     968

Cuts at:   5802    5993      33 
   Size:        191    1146

  Fragments arranged by size: 

                2309    1287    1146     968     685     282     191     141
                  68      29
 
SfoI GGC'GCC

Cuts at:   3287    4538    4786    3287 
   Size:       1251     248    5607

  Fragments arranged by size: 

                5607    1251     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7106
 
SmaI CCC'GGG

Cuts at:   4128    4128 
   Size:       7106
 
SmlI C'TyrA_G

Cuts at:   1629    2925    3484    3676    3687    4261    5176    5890    6152 
   Size:       1296     559     192      11     574     915     714     262

Cuts at:   6152    1629 
   Size:       2583

  Fragments arranged by size: 

                2583    1296     915     714     574     559     262     192
                  11
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       7106
 
SphI G_CATG'C

Cuts at:    996    1671    4159    4659     996 
   Size:        675    2488     500    3443

  Fragments arranged by size: 

                3443    2488     675     500
 
SspI AAT'ATT

Cuts at:   3405    3405 
   Size:       7106
 
StyI C'CwwG_G

Cuts at:    408     818    1502    1659    1920    2111    2481    2553    3281 
   Size:        410     684     157     261     191     370      72     728

Cuts at:   3281    4240    4594     408 
   Size:        959     354    2920

  Fragments arranged by size: 

                2920     959     728     684     410     370     354     261
                 191     157      72
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    423    1390    2869    3370    4689    4822    5281    5490     423 
   Size:        967    1479     501    1319     133     459     209    2039

  Fragments arranged by size: 

                2039    1479    1319     967     501     459     209     133
            
 
TatI w'GTAC_w

Cuts at:   1592    2147    2327    6621    1592 
   Size:        555     180    4294    2077

  Fragments arranged by size: 

                4294    2077     555     180
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1999    2031    3052    3084    6623    6655    1999 
   Size:         32    1021      32    3539      32    2450

  Fragments arranged by size: 

                3539    2450    1021      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   4903    4903 
   Size:       7106
 
XbaI T'CTAG_A

Cuts at:   4137    4137 
   Size:       7106
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1332    2419    2796    3327    4601    1332 
   Size:       1087     377     531    1274    3837

  Fragments arranged by size: 

                3837    1274    1087     531     377
 
XmaI C'CCGG_G

Cuts at:   4126    4126 
   Size:       7106
 
ZraI GAC'GTC

Cuts at:    761     761 
   Size:       7106
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NruI     NsiI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

    AflII     AgeI     AhdI     ApaI     AscI    BbvCI     BmtI   Bpu10I
    BsiWI    BsmBI    BsrGI    BstXI  BstZ17I    EcoRV     FseI     HpaI
     MfeI     NheI     NotI     PacI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     StuI     SwaI     XhoI     XmnI
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