Restriction map for pF1KA0868
 (Linear) (Six-base) MAPSORT of: KIAA0868.seq  Check: 5373  from: 1  to: 3993



KIAA0868.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:55  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1774    2300    3993 
   Size:       1774     526    1693

  Fragments arranged by size: 

                1774    1693     526
 
AatII G_ACGT'C

Cuts at:      0     655    3993 
   Size:        655    3338
 
AccI GT'mk_AC

Cuts at:      0    3098    3305    3993 
   Size:       3098     207     688

  Fragments arranged by size: 

                3098     688     207
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238    1218    1522    2569    3141    3147    3993 
   Size:        238     980     304    1047     572       6     846

  Fragments arranged by size: 

                1047     980     846     572     304     238       6
 
AfeI AGC'GCT

Cuts at:      0      35    2460    3993 
   Size:         35    2425    1533

  Fragments arranged by size: 

                2425    1533      35
 
AflIII A'CryG_T

Cuts at:      0    1649    3854    3993 
   Size:       1649    2205     139

  Fragments arranged by size: 

                2205    1649     139
 
AleI CACnn'nnGTG

Cuts at:      0     790    2563    3993 
   Size:        790    1773    1430

  Fragments arranged by size: 

                1773    1430     790
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     302    3993 
   Size:        302    3691
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1440    1472    3993 
   Size:       1440      32    2521

  Fragments arranged by size: 

                2521    1440      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      59      74    1179    1351    3993 
   Size:         59      15    1105     172    2642

  Fragments arranged by size: 

                2642    1105     172      59      15
 
ApaLI G'TGCA_C

Cuts at:      0    3828    3993 
   Size:       3828     165
 
ApoI r'AATT_y

Cuts at:      0     993    1086    1401    1593    3993 
   Size:        993      93     315     192    2400

  Fragments arranged by size: 

                2400     993     315     192      93
 
AvaI C'yCGr_G

Cuts at:      0    2889    3281    3993 
   Size:       2889     392     712

  Fragments arranged by size: 

                2889     712     392
 
AvrII C'CTAG_G

Cuts at:      0    1812    3993 
   Size:       1812    2181
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     882     915    2940    2973    3993 
   Size:        882      33    2025      33    1020

  Fragments arranged by size: 

                2025    1020     882      33      33
 
BanI G'GyrC_C

Cuts at:      0    1119    1155    2645    3060    3177    3277    3692    3871 
   Size:       1119      36    1490     415     117     100     415     179

Cuts at:   3871    3993 
   Size:        122

  Fragments arranged by size: 

                1490    1119     415     415     179     122     117     100
                  36
 
BanII G_rGCy'C

Cuts at:      0    1073    2753    3993 
   Size:       1073    1680    1240

  Fragments arranged by size: 

                1680    1240    1073
 
BbeI G_GCGC'C

Cuts at:      0    3181    3993 
   Size:       3181     812
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1224    3370    3831    3993 
   Size:       1224    2146     461     162

  Fragments arranged by size: 

                2146    1224     461     162
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1363    1397    3993 
   Size:       1363      34    2596

  Fragments arranged by size: 

                2596    1363      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1744    3993 
   Size:       1744    2249
 
BfrBI ATG'CAT

Cuts at:      0    1559    3993 
   Size:       1559    2434
 
BglI GCCn_nnn'nGGC

Cuts at:      0      79    1164    2792    3869    3993 
   Size:         79    1085    1628    1077     124

  Fragments arranged by size: 

                1628    1085    1077     124      79
 
Bme1580I G_kGCm'C

Cuts at:      0    1160    1679    3832    3874    3993 
   Size:       1160     519    2153      42     119

  Fragments arranged by size: 

                2153    1160     519     119      42
 
BmgBI CAC'GTC

Cuts at:      0      92    3606    3993 
   Size:         92    3514     387

  Fragments arranged by size: 

                3514     387      92
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     558    2160    3993 
   Size:        558    1602    1833

  Fragments arranged by size: 

                1833    1602     558
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     963    1171    2563    2767    2866    3069    3522    3993 
   Size:        963     208    1392     204      99     203     453     471

  Fragments arranged by size: 

                1392     963     471     453     208     204     203      99
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1506    2838    3361    3553    3600    3993 
   Size:       1506    1332     523     192      47     393

  Fragments arranged by size: 

                1506    1332     523     393     192      47
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     227    1228    3952    3993 
   Size:        227    1001    2724      41

  Fragments arranged by size: 

                2724    1001     227      41
 
BsaBI GATnn'nnATC

Cuts at:      0    3389    3993 
   Size:       3389     604
 
BsaHI Gr'CG_yC

Cuts at:      0     652    1031    1942    3178    3797    3923    3993 
   Size:        652     379     911    1236     619     126      70

  Fragments arranged by size: 

                1236     911     652     619     379     126      70
 
BsaWI w'CCGG_w

Cuts at:      0     338    2881    3848    3993 
   Size:        338    2543     967     145

  Fragments arranged by size: 

                2543     967     338     145
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1984    2014    2404    2434    2536    2566    2944    2974 
   Size:       1984      30     390      30     102      30     378      30

Cuts at:   2974    3187    3217    3993 
   Size:        213      30     776

  Fragments arranged by size: 

                1984     776     390     378     213     102      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     141     144     218    3160    3181    3993 
   Size:        141       3      74    2942      21     812

  Fragments arranged by size: 

                2942     812     141      74      21       3
 
BseYI C'CCAG_C

Cuts at:      0     211     973    2779    3993 
   Size:        211     762    1806    1214

  Fragments arranged by size: 

                1806    1214     762     211
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     661    2190    2413    3993 
   Size:        661    1529     223    1580

  Fragments arranged by size: 

                1580    1529     661     223
 
BsiHKAI G_wGCw'C

Cuts at:      0    1708    2753    3832    3993 
   Size:       1708    1045    1079     161

  Fragments arranged by size: 

                1708    1079    1045     161
 
BsmI GAATG_Cn'

Cuts at:      0     828    2400    3185    3993 
   Size:        828    1572     785     808

  Fragments arranged by size: 

                1572     828     808     785
 
Bsp1286I G_dGCh'C

Cuts at:      0    1073    1160    1679    1708    2753    3832    3874    3993 
   Size:       1073      87     519      29    1045    1079      42     119

  Fragments arranged by size: 

                1079    1073    1045     519     119      87      42      29
            
 
BspEI T'CCGG_A

Cuts at:      0    2881    3993 
   Size:       2881    1112
 
BspHI T'CATG_A

Cuts at:      0    1923    3932    3993 
   Size:       1923    2009      61

  Fragments arranged by size: 

                2009    1923      61
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1774    2300    2435    3993 
   Size:       1774     526     135    1558

  Fragments arranged by size: 

                1774    1558     526     135
 
BsrBI CCG'CTC

Cuts at:      0    2813    3993 
   Size:       2813    1180
 
BsrDI GCAATG_nn'

Cuts at:      0     290     632    1266    2257    2895    3800    3993 
   Size:        290     342     634     991     638     905     193

  Fragments arranged by size: 

                 991     905     638     634     342     290     193
 
BsrFI r'CCGG_y

Cuts at:      0      19    2696    3993 
   Size:         19    2677    1297

  Fragments arranged by size: 

                2677    1297      19
 
BssHII G'CGCG_C

Cuts at:      0      15    3993 
   Size:         15    3978
 
BssSI C'ACGA_G

Cuts at:      0    1375    3358    3993 
   Size:       1375    1983     635

  Fragments arranged by size: 

                1983    1375     635
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3073    3993 
   Size:       3073     920
 
BstBI TT'CG_AA

Cuts at:      0    1446    3993 
   Size:       1446    2547
 
BstEII G'GTnAC_C

Cuts at:      0     327    2615    3993 
   Size:        327    2288    1378

  Fragments arranged by size: 

                2288    1378     327
 
BstYI r'GATC_y

Cuts at:      0    1842    2211    2711    3149    3993 
   Size:       1842     369     500     438     844

  Fragments arranged by size: 

                1842     844     500     438     369
 
Bsu36I CC'TnA_GG

Cuts at:      0    1344    3993 
   Size:       1344    2649
 
BtgI C'CryG_G

Cuts at:      0    2003    2952    3993 
   Size:       2003     949    1041

  Fragments arranged by size: 

                2003    1041     949
 
BtsI GCAGTG_nn'

Cuts at:      0    2654    3993 
   Size:       2654    1339
 
ClaI AT'CG_AT

Cuts at:      0    1334    1421    3993 
   Size:       1334      87    2572

  Fragments arranged by size: 

                2572    1334      87
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3255    3290    3993 
   Size:       3255      35     703

  Fragments arranged by size: 

                3255     703      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2117    3993 
   Size:       2117    1876
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     433    1917    3993 
   Size:        433    1484    2076

  Fragments arranged by size: 

                2076    1484     433
 
EaeI y'GGCC_r

Cuts at:      0     573     780    3320    3993 
   Size:        573     207    2540     673

  Fragments arranged by size: 

                2540     673     573     207
 
EarI CTCTTCn'nnn_

Cuts at:      0    3439    3993 
   Size:       3439     554
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     870    3666    3850    3916    3993 
   Size:        870    2796     184      66      77

  Fragments arranged by size: 

                2796     870     184      77      66
 
EcoICRI GAG'CTC

Cuts at:      0    2751    3993 
   Size:       2751    1242
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     963    1171    1218    1522    2563    2569    2767 
   Size:        238     725     208      47     304    1041       6     198

Cuts at:   2767    2866    3069    3141    3147    3522    3993 
   Size:         99     203      72       6     375     471

  Fragments arranged by size: 

                1041     725     471     375     304     238     208     203
                 198      99      72      47       6       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1393    3139    3993 
   Size:       1393    1746     854

  Fragments arranged by size: 

                1746    1393     854
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1176    1867    2359    2721    3644    3993 
   Size:       1176     691     492     362     923     349

  Fragments arranged by size: 

                1176     923     691     492     362     349
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3387    3471    3594    3993 
   Size:       3387      84     123     399

  Fragments arranged by size: 

                3387     399     123      84
 
FspI TGC'GCA

Cuts at:      0     478    3993 
   Size:        478    3515
 
FspAI rTGC'GCAy

Cuts at:      0     478    3993 
   Size:        478    3515
 
HaeII r_GCGC'y

Cuts at:      0      37     849    2000    2462    3181    3993 
   Size:         37     812    1151     462     719     812

  Fragments arranged by size: 

                1151     812     812     719     462      37
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1168    1200    1327    1359    1420    1452    2343    2375 
   Size:       1168      32     127      32      61      32     891      32

Cuts at:   2375    2536    2568    2846    2878    3393    3425    3549    3581 
   Size:        161      32     278      32     515      32     124      32

Cuts at:   3581    3993 
   Size:        412

  Fragments arranged by size: 

                1168     891     515     412     278     161     127     124
                  61      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     261    1035    3345    3771    3993 
   Size:        261     774    2310     426     222

  Fragments arranged by size: 

                2310     774     426     261     222
 
HindIII A'AGCT_T

Cuts at:      0    3249    3993 
   Size:       3249     744
 
KasI G'GCGC_C

Cuts at:      0    3177    3993 
   Size:       3177     816
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2295    3449    3659    3993 
   Size:       2295    1154     210     334

  Fragments arranged by size: 

                2295    1154     334     210
 
MscI TGG'CCA

Cuts at:      0     575     782    3322    3993 
   Size:        575     207    2540     671

  Fragments arranged by size: 

                2540     671     575     207
 
MslI CAynn'nnrTG

Cuts at:      0     386     790    1573    1648    2563    3805    3993 
   Size:        386     404     783      75     915    1242     188

  Fragments arranged by size: 

                1242     915     783     404     386     188      75
 
MspA1I CmG'CkG

Cuts at:      0      59    1856    3654    3993 
   Size:         59    1797    1798     339

  Fragments arranged by size: 

                1798    1797     339      59
 
NaeI GCC'GGC

Cuts at:      0      21    3993 
   Size:         21    3972
 
NarI GG'CG_CC

Cuts at:      0    3178    3993 
   Size:       3178     815
 
NcoI C'CATG_G

Cuts at:      0    2003    3993 
   Size:       2003    1990
 
NdeI CA'TA_TG

Cuts at:      0    2985    3993 
   Size:       2985    1008
 
NgoMIV G'CCGG_C

Cuts at:      0      19    3993 
   Size:         19    3974
 
NruI TCG'CGA

Cuts at:      0    1633    3993 
   Size:       1633    2360
 
NsiI A_TGCA'T

Cuts at:      0    1561    3993 
   Size:       1561    2432
 
NspI r_CATG'y

Cuts at:      0     888    1563    1653    3858    3993 
   Size:        888     675      90    2205     135

  Fragments arranged by size: 

                2205     888     675     135      90
 
PasI CC'CwG_GG

Cuts at:      0    2495    3993 
   Size:       2495    1498
 
PciI A'CATG_T

Cuts at:      0    1649    3854    3993 
   Size:       1649    2205     139

  Fragments arranged by size: 

                2205    1649     139
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2310    3993 
   Size:       2310    1683
 
PfoI T'CCnGG_A

Cuts at:      0     408    3738    3993 
   Size:        408    3330     255

  Fragments arranged by size: 

                3330     408     255
 
PpuMI rG'GwC_Cy

Cuts at:      0    1176    2359    3993 
   Size:       1176    1183    1634

  Fragments arranged by size: 

                1634    1183    1176
 
PstI C_TGCA'G

Cuts at:      0      70    2661    3993 
   Size:         70    2591    1332

  Fragments arranged by size: 

                2591    1332      70
 
PvuII CAG'CTG

Cuts at:      0      59    3993 
   Size:         59    3934
 
SacI G_AGCT'C

Cuts at:      0    2753    3993 
   Size:       2753    1240
 
ScaI AGT'ACT

Cuts at:      0    1486    2041    2221    3993 
   Size:       1486     555     180    1772

  Fragments arranged by size: 

                1772    1486     555     180
 
SfcI C'TryA_G

Cuts at:      0      66     348    2657    2686    2754    3993 
   Size:         66     282    2309      29      68    1239

  Fragments arranged by size: 

                2309    1239     282      68      66      29
 
SfoI GGC'GCC

Cuts at:      0    3179    3993 
   Size:       3179     814
 
SmlI C'TyrA_G

Cuts at:      0    1521    2817    3376    3568    3579    3993 
   Size:       1521    1296     559     192      11     414

  Fragments arranged by size: 

                1521    1296     559     414     192      11
 
SphI G_CATG'C

Cuts at:      0     888    1563    3993 
   Size:        888     675    2430

  Fragments arranged by size: 

                2430     888     675
 
SspI AAT'ATT

Cuts at:      0    3297    3993 
   Size:       3297     696
 
StyI C'CwwG_G

Cuts at:      0     300     710    1394    1551    1812    2003    2373    2445 
   Size:        300     410     684     157     261     191     370      72

Cuts at:   2445    3173    3993 
   Size:        728     820

  Fragments arranged by size: 

                 820     728     684     410     370     300     261     191
                 157      72
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     315    1282    2761    3262    3993 
   Size:        315     967    1479     501     731

  Fragments arranged by size: 

                1479     967     731     501     315
 
TatI w'GTAC_w

Cuts at:      0    1484    2039    2219    3993 
   Size:       1484     555     180    1774

  Fragments arranged by size: 

                1774    1484     555     180
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1891    1923    2944    2976    3993 
   Size:       1891      32    1021      32    1017

  Fragments arranged by size: 

                1891    1021    1017      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1224    2311    2688    3219    3993 
   Size:       1224    1087     377     531     774

  Fragments arranged by size: 

                1224    1087     774     531     377
 
ZraI GAC'GTC

Cuts at:      0     653    3993 
   Size:        653    3340
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AleI     AlfI
     AloI    AlwNI    ApaLI     ApoI     AvaI    AvrII     BaeI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI    BfrBI     BglI Bme1580I
    BmgBI     BmrI     BpmI    BpuEI     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI   Bsu36I     BtgI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NruI     NsiI     NspI     PasI     PciI    PflMI
     PfoI    PpuMI     PstI    PvuII     SacI     ScaI     SfcI     SfoI
     SmlI     SphI     SspI     StyI    TaqII     TatI     TstI     XcmI
     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI     AhdI     ApaI     AscI     AseI
    AsiSI    BamHI    BbvCI     BclI    BglII     BlpI     BmtI     BplI
   Bpu10I    BsaAI    BsiEI    BsiWI    BsmBI    BsrGI    BstXI  BstZ17I
    BtgZI     DraI     EagI    EcoRI    EcoRV     FseI     HpaI     KpnI
     MfeI     MluI     NheI     NotI     PacI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI  Tth111I     XbaI
     XhoI     XmaI     XmnI
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