Restriction map for pF1KA0783
 (Linear) (Six-base) MAPSORT of: KIAA0783.seq  Check: 7875  from: 1  to: 2664



KIAA0783.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:55  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      15    2664 
   Size:         15    2649
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     203    1606    1703    2612    2664 
   Size:        137      66    1403      97     909      52

  Fragments arranged by size: 

                1403     909     137      97      66      52
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1401    1649    2664 
   Size:       1401     248    1015

  Fragments arranged by size: 

                1401    1015     248
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1193    1225    2664 
   Size:       1193      32    1439

  Fragments arranged by size: 

                1439    1193      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     115     421    1093    1198    2664 
   Size:        115     306     672     105    1466

  Fragments arranged by size: 

                1466     672     306     115     105
 
ApoI r'AATT_y

Cuts at:      0     246    1178    1895    2425    2664 
   Size:        246     932     717     530     239

  Fragments arranged by size: 

                 932     717     530     246     239
 
AseI AT'TA_AT

Cuts at:      0     286    2664 
   Size:        286    2378
 
BamHI G'GATC_C

Cuts at:      0    2265    2664 
   Size:       2265     399
 
BanI G'GyrC_C

Cuts at:      0    1309    2664 
   Size:       1309    1355
 
BanII G_rGCy'C

Cuts at:      0     937    2664 
   Size:        937    1727
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     749    1340    2559    2664 
   Size:        749     591    1219     105

  Fragments arranged by size: 

                1219     749     591     105
 
BclI T'GATC_A

Cuts at:      0    2205    2664 
   Size:       2205     459
 
BglII A'GATC_T

Cuts at:      0     670    2664 
   Size:        670    1994
 
Bme1580I G_kGCm'C

Cuts at:      0    1227    2664 
   Size:       1227    1437
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1364    2664 
   Size:       1364    1300
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1089    2664 
   Size:       1089    1575
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     951    1259    2651    2664 
   Size:        951     308    1392      13

  Fragments arranged by size: 

                1392     951     308      13
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     934    2664 
   Size:        934    1730
 
BsaBI GATnn'nnATC

Cuts at:      0     675     770    2210    2664 
   Size:        675      95    1440     454

  Fragments arranged by size: 

                1440     675     454      95
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     453     483    2664 
   Size:        453      30    2181

  Fragments arranged by size: 

                2181     453      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      33    2261    2664 
   Size:         33    2228     403

  Fragments arranged by size: 

                2228     403      33
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1407    2664 
   Size:       1407    1257
 
BsiHKAI G_wGCw'C

Cuts at:      0     937    1416    2106    2320    2664 
   Size:        937     479     690     214     344

  Fragments arranged by size: 

                 937     690     479     344     214
 
Bsp1286I G_dGCh'C

Cuts at:      0     937    1227    1416    2106    2320    2664 
   Size:        937     290     189     690     214     344

  Fragments arranged by size: 

                 937     690     344     290     214     189
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      15    2664 
   Size:         15    2649
 
BsrDI GCAATG_nn'

Cuts at:      0     768    2664 
   Size:        768    1896
 
BsrFI r'CCGG_y

Cuts at:      0    2088    2664 
   Size:       2088     576
 
BssSI C'ACGA_G

Cuts at:      0    2478    2664 
   Size:       2478     186
 
BstBI TT'CG_AA

Cuts at:      0    2025    2664 
   Size:       2025     639
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     545    2664 
   Size:        545    2119
 
BstYI r'GATC_y

Cuts at:      0     670    1464    2265    2664 
   Size:        670     794     801     399

  Fragments arranged by size: 

                 801     794     670     399
 
Bsu36I CC'TnA_GG

Cuts at:      0    2513    2664 
   Size:       2513     151
 
BtgI C'CryG_G

Cuts at:      0     587    2366    2664 
   Size:        587    1779     298

  Fragments arranged by size: 

                1779     587     298
 
BtsI GCAGTG_nn'

Cuts at:      0    1719    1844    2320    2349    2664 
   Size:       1719     125     476      29     315

  Fragments arranged by size: 

                1719     476     315     125      29
 
DraI TTT'AAA

Cuts at:      0      93    2664 
   Size:         93    2571
 
EaeI y'GGCC_r

Cuts at:      0    2363    2664 
   Size:       2363     301
 
EarI CTCTTCn'nnn_

Cuts at:      0     587     808     913    1445    1513    2165    2664 
   Size:        587     221     105     532      68     652     499

  Fragments arranged by size: 

                 652     587     532     499     221     105      68
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1462    2664 
   Size:       1462    1202
 
EcoICRI GAG'CTC

Cuts at:      0     935    2664 
   Size:        935    1729
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     203     951    1259    1606    1703    2612    2651 
   Size:        137      66     748     308     347      97     909      39

Cuts at:   2651    2664 
   Size:         13

  Fragments arranged by size: 

                 909     748     347     308     137      97      66      39
                  13
 
EcoRV GAT'ATC

Cuts at:      0    1773    2664 
   Size:       1773     891
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     256    1447    1975    2664 
   Size:        256    1191     528     689

  Fragments arranged by size: 

                1191     689     528     256
 
FspI TGC'GCA

Cuts at:      0    1885    2664 
   Size:       1885     779
 
FspAI rTGC'GCAy

Cuts at:      0    1885    2664 
   Size:       1885     779
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27     568     600     864     896    1398    1430    2142 
   Size:         27     541      32     264      32     502      32     712

Cuts at:   2142    2174    2664 
   Size:         32     490

  Fragments arranged by size: 

                 712     541     502     490     264      32      32      32
                  32      27
 
HincII GTy'rAC

Cuts at:      0     480    2664 
   Size:        480    2184
 
HindIII A'AGCT_T

Cuts at:      0    1957    2502    2664 
   Size:       1957     545     162

  Fragments arranged by size: 

                1957     545     162
 
HpaI GTT'AAC

Cuts at:      0     480    2664 
   Size:        480    2184
 
MfeI C'AATT_G

Cuts at:      0    1020    2664 
   Size:       1020    1644
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      78    2664 
   Size:         78    2586
 
MscI TGG'CCA

Cuts at:      0    2365    2664 
   Size:       2365     299
 
MspA1I CmG'CkG

Cuts at:      0    1444    2664 
   Size:       1444    1220
 
NcoI C'CATG_G

Cuts at:      0     587    2366    2664 
   Size:        587    1779     298

  Fragments arranged by size: 

                1779     587     298
 
NspI r_CATG'y

Cuts at:      0    1495    2664 
   Size:       1495    1169
 
PacI TTA_AT'TAA

Cuts at:      0     290    2664 
   Size:        290    2374
 
PfoI T'CCnGG_A

Cuts at:      0    1236    2664 
   Size:       1236    1428
 
PstI C_TGCA'G

Cuts at:      0     255    1851    2169    2664 
   Size:        255    1596     318     495

  Fragments arranged by size: 

                1596     495     318     255
 
SacI G_AGCT'C

Cuts at:      0     937    2664 
   Size:        937    1727
 
SapI GCTCTTCn'nnn_

Cuts at:      0     587    2664 
   Size:        587    2077
 
ScaI AGT'ACT

Cuts at:      0     266    2171    2664 
   Size:        266    1905     493

  Fragments arranged by size: 

                1905     493     266
 
SfcI C'TryA_G

Cuts at:      0     171     251     515     650    1540    1847    2165    2664 
   Size:        171      80     264     135     890     307     318     499

  Fragments arranged by size: 

                 890     499     318     307     264     171     135      80
            
 
SmlI C'TyrA_G

Cuts at:      0     913    2664 
   Size:        913    1751
 
SpeI A'CTAG_T

Cuts at:      0    1798    2664 
   Size:       1798     866
 
SspI AAT'ATT

Cuts at:      0     192    2664 
   Size:        192    2472
 
StuI AGG'CCT

Cuts at:      0    1672    2664 
   Size:       1672     992
 
StyI C'CwwG_G

Cuts at:      0     587    1111    1313    1681    2108    2366    2585    2664 
   Size:        587     524     202     368     427     258     219      79

  Fragments arranged by size: 

                 587     524     427     368     258     219     202      79
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2121    2664 
   Size:       2121     543
 
TatI w'GTAC_w

Cuts at:      0     264    1765    2169    2664 
   Size:        264    1501     404     495

  Fragments arranged by size: 

                1501     495     404     264
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     453     485    2664 
   Size:        453      32    2179

  Fragments arranged by size: 

                2179     453      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1007    1958    2537    2664 
   Size:       1007     951     579     127

  Fragments arranged by size: 

                1007     951     579     127
 

 Enzymes that do cut:

     AarI     AcuI     AlfI     AloI    AlwNI     ApoI     AseI    BamHI
     BanI    BanII     BbsI     BclI    BglII Bme1580I     BmrI     BplI
     BpmI    BpuEI    BsaBI    BsaXI    BseRI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrDI    BsrFI    BssSI    BstBI    BstXI    BstYI   Bsu36I
     BtgI     BtsI     DraI     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoRV     FalI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     MfeI     MmeI     MscI   MspA1I     NcoI     NspI     PacI     PfoI
     PstI     SacI     SapI     ScaI     SfcI     SmlI     SpeI     SspI
     StuI     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI
    BmgBI     BmtI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BseYI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrGI
   BssHII   BstAPI   BstEII  BstZ17I    BtgZI     ClaI    CspCI   DraIII
     DrdI     EagI    EcoNI EcoO109I    EcoRI     FseI    HaeII     KasI
     KpnI     MluI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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