Restriction map for pF1KA0767
##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AclI        AcsI        AflII       AgeI        AhdI        
AlwI        AocI        ApaI        ApoI        AscI        
AseI        AsnI        AspEI       AviII       AvrII       
BamHI       BbeI        BbrPI       BcgI        BclI        
BfrI        BlnI        BsaBI       BsiWI       BspDI       
BspHI       BspLU11I    Bst1107I    BstXI       BstZ17I     
Bsu36I      ClaI        CpoI        DraIII      Eam1105I    
EarI        Eco81I      EcoRI       EcoRV       EcoT22I     
FbaI        FspI        HindIII     KasI        Ksp632I     
MamI        MfeI        MluI        MunI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SpeI        
SphI        Sse8387I    SspI        SwaI        TfiI        
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   69 enzymes) #
###################################

AatII         910  GACGTC               GACGT^C
Acc65I        117  GGTACC               G^GTACC
AccIII        293  TCCGGA               T^CCGGA
AflIII        695  ACACGT               A^C[GA][CT]GT
Alw44I       1654  GTGCAC               G^TGCAC
Aor51HI      1211  AGCGCT               AGC^GCT
ApaLI        1654  GTGCAC               G^TGCAC
Asp718I       117  GGTACC               G^GTACC
BalI          185  TGGCCA               TGG^CCA
BbsI          871  GAAGAC               GAAGAC(2/6)
BciVI         818  GGATAC               GTATCC(6/5)
BglI         1033  GCCTTCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1346  AGATCT               A^GATCT
BlpI          421  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      421  GCTCAGC              GC^T[ACGT]AGC
BpuAI         871  GAAGAC               GAAGAC(2/6)
BsaI         1675  GAGACC               GGTCTC(1/5)
BseAI         293  TCCGGA               T^CCGGA
BsgI          382  GTGCAG               GTGCAG(16/14)
BsmBI         615  GAGACG               CGTCTC(1/5)
Bsp1407I      719  TGTACA               T^GTACA
BspEI         293  TCCGGA               T^CCGGA
BspMI         596  GCAGGT               ACCTGC(4/8)
BsrGI         719  TGTACA               T^GTACA
BssHII       1146  GCGCGC               G^CGCGC
BstBI        1489  TTCGAA               TT^CGAA
BstEII        244  GGTCACC              G^GT[ACGT]ACC
BstPI         244  GGTCACC              G^GT[ACGT]ACC
BstYI        1346  AGATCT               [GA]^GATC[CT]
CelII         421  GCTCAGC              GC^T[ACGT]AGC
DraI         1126  TTTAAA               TTT^AAA
DrdI         1245  GACGACCTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1280  CGGCCG               C^GGCCG
EclXI        1280  CGGCCG               C^GGCCG
Eco47III     1211  AGCGCT               AGC^GCT
Eco52I       1280  CGGCCG               C^GGCCG
EcoNI        1511  CCTCAAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       244  GGTCACC              G^GT[ACGT]ACC
EcoT14I      1649  CCATGG               C^C[AT][AT]GG
FseI         1281  GGCCGGCC             GGCCGG^CC
HpaI          600  GTTAAC               GTT^AAC
KpnI          117  GGTACC               GGTAC^C
KspI          121  CCGCGG               CCGC^GG
MflI         1346  AGATCT               [GA]^GATC[CT]
MluNI         185  TGGCCA               TGG^CCA
MroI          293  TCCGGA               T^CCGGA
MscI          185  TGGCCA               TGG^CCA
MslI         1657  CACAGGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI         1282  GCCGGC               GCC^GGC
NcoI         1649  CCATGG               C^CATGG
NgoMIV       1282  GCCGGC               G^CCGGC
NspV         1489  TTCGAA               TT^CGAA
PflMI         806  CCAGGGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1303  GACGCCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         898  CAGCTG               CAG^CTG
SacI          289  GAGCTC               GAGCT^C
SacII         121  CCGCGG               CCGC^GG
SalI         1611  GTCGAC               G^TCGAC
SfuI         1489  TTCGAA               TT^CGAA
SmaI          134  CCCGGG               CCC^GGG
SnaBI        1264  TACGTA               TAC^GTA
SspBI         719  TGTACA               T^GTACA
StuI         1667  AGGCCT               AGG^CCT
StyI         1649  CCATGG               C^C[AT][AT]GG
Van91I        806  CCAGGGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          447  CCAGGCCAGCAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1346  AGATCT               [GA]^GATC[CT]
XmaCI         134  CCCGGG               C^CCGGG
XmaI          134  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI          691  GTCTAC               GT^[AC][GT]AC
             1611  GTCGAC              
AspI          879  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1248  GACCTCGTC           
BanII         289  GAGCTC               G[GA]GC[CT]^C
              850  GAGCCC              
BbvCI        1706  CCTCAGC              CCTCAGC(-5/-2)
              150  GCTGAGG             
BcnI          134  CCCGG                CC^[GC]GG
              135  CCGGG               
BsaAI         529  TACGTG               [CT]AC^GT[GA]
             1264  TACGTA              
BsiEI        1280  CGGCCG               CG[GA][CT]^CG
             1308  CGGTCG              
BsmI           91  GAATGC               GAATGC(1/-1)
              726  GAATGC              
BsrDI         705  CATTGC               GCAATG(2/0)
              455  GCAATG              
BsrI          680  ACTGG                ACTGG(1/-1)
             1689  CCAGT               
BssSI        1342  CACGAG               CACGAG(-5/-1)
             1636  CTCGTG              
BstAPI        375  GCAGAAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              384  GCAGGAGGTGC         
EaeI          185  TGGCCA               [CT]^GGCC[GA]
             1280  CGGCCG              
HincII        600  GTTAAC               GT[CT]^[GA]AC
             1611  GTCGAC              
HindII        600  GTTAAC               GT[CT]^[GA]AC
             1611  GTCGAC              
NciI          134  CCCGG                CC^[GC]GG
              135  CCGGG               
PflFI         879  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1248  GACCTCGTC           
PstI          496  CTGCAG               CTGCA^G
             1240  CTGCAG              
SfcI          496  CTGCAG               C^T[GA][CT]AG
             1240  CTGCAG              
SmlI          622  CTCAAG               C^T[CT][GA]AG
             1204  CTCAAG              
TspRI         564  CACTG                CA[GC]TG[ACGT][ACGT]^
              823  CAGTG               
Tth111I       879  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1248  GACCTCGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       Asp700I     AspHI       AvaI        AvaII       
BanI        BbvI        BfaI        BmyI        BpmI        
BsaHI       BsaJI       BsaWI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrBI       BsrFI       BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MspA1I      MspI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TaqI        Tru9I       
TseI        Tsp45I      Tsp509I     TthHB8I     XmnI        
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