Restriction map for pF1KA0621
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       AclI        AcyI        AflII       AflIII      
AgeI        Alw44I      ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     AvrII       BamHI       
BbeI        BbrPI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BseRI       BsiEI       BsiWI       BspDI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BssSI       Bst1107I    BstEII      BstPI       BstXI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraIII      EagI        EclXI       Eco52I      EcoNI       
EcoO65I     EcoRI       FauI        FbaI        FseI        
Hin1I       KasI        KspI        MamI        MfeI        
MluI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NspI        
PacI        PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SphI        Sse8387I    SspI        XbaI        XcmI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

Acc65I        900  GGTACC               G^GTACC
AccII         144  CGCG                 CG^CG
AccIII       1997  TCCGGA               T^CCGGA
AlwNI          24  CAGCGACTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI        12  AGCGCT               AGC^GCT
Asp718I       900  GGTACC               G^GTACC
AspI          502  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1146  TGCGCA               TGC^GCA
BalI          650  TGGCCA               TGG^CCA
BanI          900  GGTACC               G^G[CT][GA]CC
BbvCI        1608  GCTGAGG              CCTCAGC(-5/-2)
BsaI         1455  GGTCTC               GGTCTC(1/5)
BseAI        1997  TCCGGA               T^CCGGA
BsmBI          58  GAGACG               CGTCTC(1/5)
Bsp1407I      865  TGTACA               T^GTACA
BspEI        1997  TCCGGA               T^CCGGA
BsrGI         865  TGTACA               T^GTACA
BssHII        143  GCGCGC               G^CGCGC
BstBI         356  TTCGAA               TT^CGAA
BstUI         144  CGCG                 CG^CG
BstYI        1673  AGATCC               [GA]^GATC[CT]
DraI          199  TTTAAA               TTT^AAA
DraII        1795  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DrdI           60  GACGCTCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III       12  AGCGCT               AGC^GCT
EcoO109I     1795  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRV        1315  GATATC               GAT^ATC
EcoT22I      1185  ATGCAT               ATGCA^T
FspI         1146  TGCGCA               TGC^GCA
HaeII          12  AGCGCT               [GA]GCGC^[CT]
HindIII      1049  AAGCTT               A^AGCTT
HpaI          681  GTTAAC               GTT^AAC
KpnI          900  GGTACC               GGTAC^C
MflI         1673  AGATCC               [GA]^GATC[CT]
MluNI         650  TGGCCA               TGG^CCA
MroI         1997  TCCGGA               T^CCGGA
MscI          650  TGGCCA               TGG^CCA
MslI          517  CATTTCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          144  CGCG                 CG^CG
NsiI         1185  ATGCAT               ATGCA^T
NspV          356  TTCGAA               TT^CGAA
PaeR7I         16  CTCGAG               C^TCGAG
PflFI         502  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1191  CCATGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI         1785  CTGCAG               CTGCA^G
SacI          112  GAGCTC               GAGCT^C
SexAI         503  ACCTGGT              A^CC[AT]GGT
SfcI         1785  CTGCAG               C^T[GA][CT]AG
SfuI          356  TTCGAA               TT^CGAA
SpeI         1348  ACTAGT               A^CTAGT
SspBI         865  TGTACA               T^GTACA
StuI         1612  AGGCCT               AGG^CCT
SwaI          198  ATTTAAAT             ATTT^AAAT
Tth111I       502  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1191  CCATGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI           16  CTCGAG               C^TCGAG
XhoII        1673  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI         1021  GTAGAC               GT^[AC][GT]AC
             1108  GTCTAC              
AlwI         1674  GATCC                GGATC(4/5)
             1211  GGATC               
AocI          279  CCTCAGG              CC^T[ACGT]AGG
             1615  CCTCAGG             
AvaI           16  CTCGAG               C^[CT]CG[GA]G
             1451  CTCGGG              
BanII           4  GGGCTC               G[GA]GC[CT]^C
              112  GAGCTC              
BcgI         1743  GCACCTGTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2063  GCACGGTCTTCG        
BcnI         1098  CCGGG                CC^[GC]GG
             1973  CCCGG               
BfaI          725  CTAG                 C^TAG
             1349  CTAG                
BsaAI         817  TACGTG               [CT]AC^GT[GA]
             2152  TACGTG              
BsaWI        1067  ACCGGA               [AT]^CCGG[AT]
             1997  TCCGGA              
BsmI          318  GAATGC               GAATGC(1/-1)
             1373  GAATGC              
BsoBI          16  CTCGAG               C^[CT]CG[GA]G
             1451  CTCGGG              
BspHI        1391  TCATGA               T^CATGA
             1520  TCATGA              
BstAPI       1260  GCAGAAGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1534  GCAAATGTTGC         
Bsu36I        279  CCTCAGG              CC^T[ACGT]AGG
             1615  CCTCAGG             
EaeI          650  TGGCCA               [CT]^GGCC[GA]
             1095  TGGCCG              
Eco81I        279  CCTCAGG              CC^T[ACGT]AGG
             1615  CCTCAGG             
HgaI           60  GACGC                GACGC(5/10)
             1803  GACGC               
MaeI          725  CTAG                 C^TAG
             1349  CTAG                
MspA1I       1513  CAGCTG               C[AC]G^C[GT]G
             1738  CAGCTG              
NciI         1098  CCGGG                CC^[GC]GG
             1973  CCCGG               
PvuII        1513  CAGCTG               CAG^CTG
             1738  CAGCTG              
RcaI         1391  TCATGA               T^CATGA
             1520  TCATGA              
SapI         1758  GAAGAGC              GCTCTTC(1/4)
             1806  GCTCTTC             


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AcsI        AfaI        AhdI        AluI        
ApoI        AspEI       AspHI       AvaII       BbsI        
BbvI        BciVI       BmyI        BpmI        BpuAI       
BsaJI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DsaI        Eam1105I    EarI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MspI        MvaI        MwoI        NcoI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     
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