Restriction map for pF1KA0600
 (Circular) (Six-base) MAPSORT of: pF1KA0600.seq  Check: 7094  from: 1  to: 6224



pF1KA0600  6224 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2016    3073    2016 
   Size:       1057    5167
 
AccI GT'mk_AC

Cuts at:   1288    3262    1288 
   Size:       1974    4250
 
Acc65I G'GTAC_C

Cuts at:   3240    3240 
   Size:       6224
 
AclI AA'CG_TT

Cuts at:   6064    6064 
   Size:       6224
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2594    2658    4049    4481    4834    5496    2594 
   Size:         64    1391     432     353     662    3322

  Fragments arranged by size: 

                3322    1391     662     432     353      64
 
AflIII A'CryG_T

Cuts at:   1199    1533    3576    5376    5657    1199 
   Size:        334    2043    1800     281    1766

  Fragments arranged by size: 

                2043    1800    1766     334     281
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2286    3227    2286 
   Size:        941    5283
 
AlwNI CAG_nnn'CTG

Cuts at:    333     372     387     528     585     597     883    1326    1629 
   Size:         39      15     141      57      12     286     443     303

Cuts at:   1629    1638    1668    2013    2073    2563    3529    4967     333 
   Size:          9      30     345      60     490     966    1438    1590

  Fragments arranged by size: 

                1590    1438     966     490     443     345     303     286
                 141      60      57      39      30      15      12       9
            
 
ApaI G_GGCC'C

Cuts at:    842    1958     842 
   Size:       1116    5108
 
ApaLI G'TGCA_C

Cuts at:   5062    5062 
   Size:       6224
 
ApoI r'AATT_y

Cuts at:    673    3228    5577    5833     673 
   Size:       2555    2349     256    1064

  Fragments arranged by size: 

                2555    2349    1064     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6224
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6224
 
AvaI C'yCGr_G

Cuts at:   1823    3164    3244    1823 
   Size:       1341      80    4803

  Fragments arranged by size: 

                4803    1341      80
 
AvrII C'CTAG_G

Cuts at:   1227    1227 
   Size:       6224
 
BamHI G'GATC_C

Cuts at:   3249    3249 
   Size:       6224
 
BanI G'GyrC_C

Cuts at:   1012    2037    2479    3240    3654    3902    3937    1012 
   Size:       1025     442     761     414     248      35    3299

  Fragments arranged by size: 

                3299    1025     761     442     414     248      35
 
BanII G_rGCy'C

Cuts at:    294     304     347     701     756     842    1161    1420    1812 
   Size:         10      43     354      55      86     319     259     392

Cuts at:   1812    1834    1958    2122    2312    2571    3197    3238    4268 
   Size:         22     124     164     190     259     626      41    1030

Cuts at:   4268     294 
   Size:       2250

  Fragments arranged by size: 

                2250    1030     626     392     354     319     259     259
                 190     164     124      86      55      43      41      22
                  10
 
BbeI G_GCGC'C

Cuts at:   2041    3658    3906    2041 
   Size:       1617     248    4359

  Fragments arranged by size: 

                4359    1617     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1865    1889    1865 
   Size:         24    6200
 
BbvCI CC'TCA_GC

Cuts at:    661    1148    1946    2044     661 
   Size:        487     798      98    4841

  Fragments arranged by size: 

                4841     798     487      98
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1924    1958    1924 
   Size:         34    6190
 
BciVI GTATCCnnnnn_n'

Cuts at:   1975    4122    5178    6224    1975 
   Size:       2147    1056    1046    1975

  Fragments arranged by size: 

                2147    1975    1056    1046
 
BclI T'GATC_A

Cuts at:   3744    3744 
   Size:       6224
 
BglI GCCn_nnn'nGGC

Cuts at:   1391    2899    3145    1391 
   Size:       1508     246    4470

  Fragments arranged by size: 

                4470    1508     246
 
BglII A'GATC_T

Cuts at:   3739    5776    3739 
   Size:       2037    4187
 
BlpI GC'TnA_GC

Cuts at:    929    3336     929 
   Size:       2407    3817
 
Bme1580I G_kGCm'C

Cuts at:    842    1958    2063    3849    3942    5066     842 
   Size:       1116     105    1786      93    1124    2000

  Fragments arranged by size: 

                2000    1786    1124    1116     105      93
 
BmgBI CAC'GTC

Cuts at:   1202    2655    1202 
   Size:       1453    4771
 
BmrI ACTGGGnnnn_n'

Cuts at:    230    2079    2466    3436    3517    3852    5996     230 
   Size:       1849     387     970      81     335    2144     458

  Fragments arranged by size: 

                2144    1849     970     458     387     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    800    1610    2256    4591     800 
   Size:        810     646    2335    2433

  Fragments arranged by size: 

                2433    2335     810     646
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    550     592     607     652    1303    1423    1480    1690    1885 
   Size:         42      15      45     651     120      57     210     195

Cuts at:   1885    2229    2299    2878    3045     550 
   Size:        344      70     579     167    3729

  Fragments arranged by size: 

                3729     651     579     344     210     195     167     120
                  70      57      45      42      15
 
Bpu10I CC'TnA_GC

Cuts at:    661    1148    1585    1946    2044     661 
   Size:        487     437     361      98    4841

  Fragments arranged by size: 

                4841     487     437     361      98
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    340     442    2296    3400    4279    4993    5291     340 
   Size:        102    1854    1104     879     714     298    1273

  Fragments arranged by size: 

                1854    1273    1104     879     714     298     102
 
BsaI GGTCTCn'nnnn_

Cuts at:   3082    5928    3082 
   Size:       2846    3378
 
BsaAI yAC'GTr

Cuts at:   3577    3577 
   Size:       6224
 
BsaBI GATnn'nnATC

Cuts at:      6    3738    5775       6 
   Size:       3732    2037     455

  Fragments arranged by size: 

                3732    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    808    1027    2038    3655    3903    4605    6101     808 
   Size:        219    1011    1617     248     702    1496     931

  Fragments arranged by size: 

                1617    1496    1011     931     702     248     219
 
BsaWI w'CCGG_w

Cuts at:   3413    3934    4612    5023    5170    5444    3413 
   Size:        521     678     411     147     274    4193

  Fragments arranged by size: 

                4193     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    927     957     927 
   Size:         30    6194
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1771    1780    1783    1882    1885    1909    1912    1915    1942 
   Size:          9       3      99       3      24       3       3      27

Cuts at:   1942    1963    1981    2232    2756    3109    3205    1771 
   Size:         21      18     251     524     353      96    4790

  Fragments arranged by size: 

                4790     524     353     251      99      96      27      24
                  21      18       9       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:    268     746    1063    1389    1698    1797    2006    2483    3649 
   Size:        478     317     326     309      99     209     477    1166

Cuts at:   3649    4407    5072    5480     268 
   Size:        758     665     408    1012

  Fragments arranged by size: 

                1166    1012     758     665     478     477     408     326
                 317     309     209      99
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    166    3055     166 
   Size:       2889    3335
 
BsiEI CG_ry'CG

Cuts at:    104    3766    3812    5042     104 
   Size:       3662      46    1230    1286

  Fragments arranged by size: 

                3662    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    347    1366    1483    1852    2312    2516    3238    4016    4206 
   Size:       1019     117     369     460     204     722     778     190

Cuts at:   4206    5066     347 
   Size:        860    1505

  Fragments arranged by size: 

                1505    1019     860     778     722     460     369     204
                 190     117
 
BsmBI CGTCTCn'nnnn_

Cuts at:    848    1208    2645     848 
   Size:        360    1437    4427

  Fragments arranged by size: 

                4427    1437     360
 
Bsp1286I G_dGCh'C

Cuts at:    294     304     347     701     756     842    1161    1366    1420 
   Size:         10      43     354      55      86     319     205      54

Cuts at:   1420    1483    1812    1834    1852    1958    2063    2122    2312 
   Size:         63     329      22      18     106     105      59     190

Cuts at:   2312    2516    2571    3197    3238    3849    3942    4016    4206 
   Size:        204      55     626      41     611      93      74     190

Cuts at:   4206    4268    5066     294 
   Size:         62     798    1452

  Fragments arranged by size: 

                1452     798     626     611     354     329     319     205
                 204     190     190     106     105      93      86      74
                  63      62      59      55      55      54      43      41
                  22      18      10
 
BspHI T'CATG_A

Cuts at:   1358    4656    1358 
   Size:       3298    2926
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2016    3073    3274    3488    3790    4171    2016 
   Size:       1057     201     214     302     381    4069

  Fragments arranged by size: 

                4069    1057     381     302     214     201
 
BsrBI CCG'CTC

Cuts at:     50     794     933    4516    4570    6054    6219      50 
   Size:        744     139    3583      54    1484     165      55

  Fragments arranged by size: 

                3583    1484     744     165     139      55      54
 
BsrDI GCAATG_nn'

Cuts at:    638    1487    2481    2693    4136     638 
   Size:        849     994     212    1443    2726

  Fragments arranged by size: 

                2726    1443     994     849     212
 
BsrFI r'CCGG_y

Cuts at:    213    1348    3059    3183    4222    4403     213 
   Size:       1135    1711     124    1039     181    2034

  Fragments arranged by size: 

                2034    1711    1135    1039     181     124
 
BssSI C'ACGA_G

Cuts at:   2701    4495    5203    2701 
   Size:       1794     708    3722

  Fragments arranged by size: 

                3722    1794     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:    333     372     474     501     597    1449    2022    3601     333 
   Size:         39     102      27      96     852     573    1579    2956

  Fragments arranged by size: 

                2956    1579     852     573     102      96      39      27
            
 
BstBI TT'CG_AA

Cuts at:   4585    4585 
   Size:       6224
 
BstXI CCAn_nnnn'nTGG

Cuts at:    243     276    2370    2476     243 
   Size:         33    2094     106    3991

  Fragments arranged by size: 

                3991    2094     106      33
 
BstYI r'GATC_y

Cuts at:      1    1706    3008    3249    3716    3739    4074    4320    4626 
   Size:       1705    1302     241     467      23     335     246     306

Cuts at:   4626    4638    4724    5776       1 
   Size:         12      86    1052     449

  Fragments arranged by size: 

                1705    1302    1052     467     449     335     306     246
                 241      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1754    1754 
   Size:       6224
 
BtgI C'CryG_G

Cuts at:    169     242    1241    1422    1503    1570    2369    2469    3116 
   Size:         73     999     181      81      67     799     100     647

Cuts at:   3116    3197     169 
   Size:         81    3196

  Fragments arranged by size: 

                3196     999     799     647     181     100      81      81
                  73      67
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1077    2515    4174    4355    4518    1077 
   Size:       1438    1659     181     163    2783

  Fragments arranged by size: 

                2783    1659    1438     181     163
 
BtsI GCAGTG_nn'

Cuts at:    316    1268    2270    2987    3467     316 
   Size:        952    1002     717     480    3073

  Fragments arranged by size: 

                3073    1002     952     717     480
 
DraI TTT'AAA

Cuts at:   3223    3223 
   Size:       6224
 
DraIII CAC_nnn'GTG

Cuts at:   2225    2225 
   Size:       6224
 
DrdI GACnn_nn'nnGTC

Cuts at:   2134    3930    5274    2134 
   Size:       1796    1344    3084

  Fragments arranged by size: 

                3084    1796    1344
 
EaeI y'GGCC_r

Cuts at:   1538    2051    2802    2984    3809    3983    4374    4401    5746 
   Size:        513     751     182     825     174     391      27    1345

Cuts at:   5746    1538 
   Size:       2016

  Fragments arranged by size: 

                2016    1345     825     751     513     391     182     174
                  27
 
EagI C'GGCC_G

Cuts at:   3809    3809 
   Size:       6224
 
EarI CTCTTCn'nnn_

Cuts at:    560     616     985    2110    2152    2570    4247    4457     560 
   Size:         56     369    1125      42     418    1677     210    2327

  Fragments arranged by size: 

                2327    1677    1125     418     369     210      56      42
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2721    4432    5174    5320    6034    2721 
   Size:       1711     742     146     714    2911

  Fragments arranged by size: 

                2911    1711     742     714     146
 
EcoICRI GAG'CTC

Cuts at:    345    2310    3236     345 
   Size:       1965     926    3333

  Fragments arranged by size: 

                3333    1965     926
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    550     592     607     652    1303    1423    1480    1690    1885 
   Size:         42      15      45     651     120      57     210     195

Cuts at:   1885    2229    2299    2594    2658    2878    3045    4049    4481 
   Size:        344      70     295      64     220     167    1004     432

Cuts at:   4481    4834    5496     550 
   Size:        353     662    1278

  Fragments arranged by size: 

                1278    1004     662     651     432     353     344     295
                 220     210     195     167     120      70      64      57
                  45      42      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1330    1867    3070    1330 
   Size:        537    1203    4484

  Fragments arranged by size: 

                4484    1203     537
 
EcoO109I rG'GnC_Cy

Cuts at:    839    1314    1626    1954    2070    2158    2442    3363     839 
   Size:        475     312     328     116      88     284     921    3700

  Fragments arranged by size: 

                3700     921     475     328     312     284     116      88
            
 
EcoRI G'AATT_C

Cuts at:    673    3228     673 
   Size:       2555    3669
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2163    4243    2163 
   Size:       2080    4144
 
FspI TGC'GCA

Cuts at:   2257    4005    2257 
   Size:       1748    4476
 
FspAI rTGC'GCAy

Cuts at:   2257    2257 
   Size:       6224
 
HaeII r_GCGC'y

Cuts at:    355    2041    3116    3658    3906    5136    5907     355 
   Size:       1686    1075     542     248    1230     771     672

  Fragments arranged by size: 

                1686    1230    1075     771     672     542     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    121     126     153     158     927     959    1131    1163    2312 
   Size:          5      27       5     769      32     172      32    1149

Cuts at:   2312    2344    4066    4096    4098    4128     121 
   Size:         32    1722      30       2      30    2217

  Fragments arranged by size: 

                2217    1722    1149     769     172      32      32      32
                  30      30      27       5       5       2
 
HincII GTy'rAC

Cuts at:   1289    3263    1289 
   Size:       1974    4250
 
KasI G'GCGC_C

Cuts at:   2037    3654    3902    2037 
   Size:       1617     248    4359

  Fragments arranged by size: 

                4359    1617     248
 
KpnI G_GTAC'C

Cuts at:   3244    3244 
   Size:       6224
 
MluI A'CGCG_T

Cuts at:   5657    5657 
   Size:       6224
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    107    4983    5167     107 
   Size:       4876     184    1164

  Fragments arranged by size: 

                4876    1164     184
 
MscI TGG'CCA

Cuts at:   1540    2053    2804    2986    3985    1540 
   Size:        513     751     182     999    3779

  Fragments arranged by size: 

                3779     999     751     513     182
 
MslI CAynn'nnrTG

Cuts at:    193    1138    1427    1492    1546    2206    2263    2368    2474 
   Size:        945     289      65      54     660      57     105     106

Cuts at:   2474    2534    4213    4340     193 
   Size:         60    1679     127    2077

  Fragments arranged by size: 

                2077    1679     945     660     289     127     106     105
                  65      60      57      54
 
MspA1I CmG'CkG

Cuts at:    171     372     444     540     546     558     582     597    1572 
   Size:        201      72      96       6      12      24      15     975

Cuts at:   1572    1608    1638    1698    2013    2820    3049    3335    3649 
   Size:         36      30      60     315     807     229     286     314

Cuts at:   3649    4009    4793    5038     171 
   Size:        360     784     245    1357

  Fragments arranged by size: 

                1357     975     807     784     360     315     314     286
                 245     229     201      96      72      60      36      30
                  24      15      12       6
 
NaeI GCC'GGC

Cuts at:   3185    4405    3185 
   Size:       1220    5004
 
NarI GG'CG_CC

Cuts at:   2038    3655    3903    2038 
   Size:       1617     248    4359

  Fragments arranged by size: 

                4359    1617     248
 
NcoI C'CATG_G

Cuts at:    242    2369    2469    3116    3197     242 
   Size:       2127     100     647      81    3269

  Fragments arranged by size: 

                3269    2127     647     100      81
 
NgoMIV G'CCGG_C

Cuts at:   3183    4403    3183 
   Size:       1220    5004
 
NspI r_CATG'y

Cuts at:   1432    1450    1551    3277    3777    5380    1432 
   Size:         18     101    1726     500    1603    2276

  Fragments arranged by size: 

                2276    1726    1603     500     101      18
 
PasI CC'CwG_GG

Cuts at:    802    2073    2104     802 
   Size:       1271      31    4922

  Fragments arranged by size: 

                4922    1271      31
 
PciI A'CATG_T

Cuts at:   5376    5376 
   Size:       6224
 
PflMI CCAn_nnn'nTGG

Cuts at:    746    2073    2563     746 
   Size:       1327     490    4407

  Fragments arranged by size: 

                4407    1327     490
 
PfoI T'CCnGG_A

Cuts at:    668    1667     668 
   Size:        999    5225
 
PmeI GTTT'AAAC

Cuts at:   3223    3223 
   Size:       6224
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4670    4702    4670 
   Size:         32    6192
 
PpuMI rG'GwC_Cy

Cuts at:   2442    2442 
   Size:       6224
 
PshAI GACnn'nnGTC

Cuts at:   2760    2760 
   Size:       6224
 
PspOMI G'GGCC_C

Cuts at:    838    1954     838 
   Size:       1116    5108
 
PstI C_TGCA'G

Cuts at:    338     377     449    1238    1445    1634    2018    2304    2936 
   Size:         39      72     789     207     189     384     286     632

Cuts at:   2936    3271    3956     338 
   Size:        335     685    2606

  Fragments arranged by size: 

                2606     789     685     632     384     335     286     207
                 189      72      39
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6224
 
PvuII CAG'CTG

Cuts at:    372     444     597    1638    1698    2820    3649    4009     372 
   Size:         72     153    1041      60    1122     829     360    2587

  Fragments arranged by size: 

                2587    1122    1041     829     360     153      72      60
            
 
RsrII CG'GwC_CG

Cuts at:   4419    4419 
   Size:       6224
 
SacI G_AGCT'C

Cuts at:    347    2312    3238     347 
   Size:       1965     926    3333

  Fragments arranged by size: 

                3333    1965     926
 
SacII CC_GC'GG

Cuts at:    172    1573     172 
   Size:       1401    4823
 
SalI G'TCGA_C

Cuts at:   1287    3261    1287 
   Size:       1974    4250
 
SapI GCTCTTCn'nnn_

Cuts at:    560     616    2110    4247    4457     560 
   Size:         56    1494    2137     210    2327

  Fragments arranged by size: 

                2327    2137    1494     210      56
 
SbfI CC_TGCA'GG

Cuts at:   3271    3271 
   Size:       6224
 
ScaI AGT'ACT

Cuts at:   5741    5741 
   Size:       6224
 
SfcI C'TryA_G

Cuts at:     33     334     373     445    1234    1441    1630    1693    2014 
   Size:        301      39      72     789     207     189      63     321

Cuts at:   2014    2300    2393    2416    2932    3267    3952    4920    5111 
   Size:        286      93      23     516     335     685     968     191

Cuts at:   5111      33 
   Size:       1146

  Fragments arranged by size: 

                1146     968     789     685     516     335     321     301
                 286     207     191     189      93      72      63      39
                  23
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   3145    3145 
   Size:       6224
 
SfoI GGC'GCC

Cuts at:   2039    3656    3904    2039 
   Size:       1617     248    4359

  Fragments arranged by size: 

                4359    1617     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6224
 
SmaI CCC'GGG

Cuts at:   1825    3166    3246    1825 
   Size:       1341      80    4803

  Fragments arranged by size: 

                4803    1341      80
 
SmlI C'TyrA_G

Cuts at:    355     457    2311    3379    4294    5008    5270     355 
   Size:        102    1854    1068     915     714     262    1309

  Fragments arranged by size: 

                1854    1309    1068     915     714     262     102
 
SpeI A'CTAG_T

Cuts at:     67    1518      67 
   Size:       1451    4773
 
SphI G_CATG'C

Cuts at:   1432    1551    3277    3777    1432 
   Size:        119    1726     500    3879

  Fragments arranged by size: 

                3879    1726     500     119
 
StyI C'CwwG_G

Cuts at:    155     242    1227    2369    2469    2939    3116    3197    3358 
   Size:         87     985    1142     100     470     177      81     161

Cuts at:   3358    3712     155 
   Size:        354    2667

  Fragments arranged by size: 

                2667    1142     985     470     354     177     161     100
                  87      81
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1762    2497    2761    3106    3807    3940    4399    4608    1762 
   Size:        735     264     345     701     133     459     209    3378

  Fragments arranged by size: 

                3378     735     701     459     345     264     209     133
            
 
TatI w'GTAC_w

Cuts at:   5739    5739 
   Size:       6224
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1495    1527    2582    2614    2616    2648    5741    5773    1495 
   Size:         32    1055      32       2      32    3093      32    1946

  Fragments arranged by size: 

                3093    1946    1055      32      32      32      32       2
            
 
Tth111I GACn'n_nGTC

Cuts at:   4021    4021 
   Size:       6224
 
XbaI T'CTAG_A

Cuts at:   3255    3255 
   Size:       6224
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2652    3719    2652 
   Size:       1067    5157
 
XmaI C'CCGG_G

Cuts at:   1823    3164    3244    1823 
   Size:       1341      80    4803

  Fragments arranged by size: 

                4803    1341      80
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    FspAI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI    PshAI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AgeI     AhdI     AleI     AloI     AscI
     BaeI    BfrBI     BmtI    BsiWI     BsmI    BspEI    BsrGI   BssHII
   BstEII  BstZ17I     ClaI    CspCI    EcoRV     FseI  HindIII     HpaI
     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI     PmlI
     PsiI    PspXI     PsrI    SanDI    SexAI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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