Restriction map for pF1KA0600
 (Linear) (Six-base) MAPSORT of: KIAA0600.seq  Check: 4668  from: 1  to: 3111



KIAA0600.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:54  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1908    2965    3111 
   Size:       1908    1057     146

  Fragments arranged by size: 

                1908    1057     146
 
AccI GT'mk_AC

Cuts at:      0    1180    3111 
   Size:       1180    1931
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2486    2550    3111 
   Size:       2486      64     561

  Fragments arranged by size: 

                2486     561      64
 
AflIII A'CryG_T

Cuts at:      0    1091    1425    3111 
   Size:       1091     334    1686

  Fragments arranged by size: 

                1686    1091     334
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2178    3111 
   Size:       2178     933
 
AlwNI CAG_nnn'CTG

Cuts at:      0     225     264     279     420     477     489     775    1218 
   Size:        225      39      15     141      57      12     286     443

Cuts at:   1218    1521    1530    1560    1905    1965    2455    3111 
   Size:        303       9      30     345      60     490     656

  Fragments arranged by size: 

                 656     490     443     345     303     286     225     141
                  60      57      39      30      15      12       9
 
ApaI G_GGCC'C

Cuts at:      0     734    1850    3111 
   Size:        734    1116    1261

  Fragments arranged by size: 

                1261    1116     734
 
ApoI r'AATT_y

Cuts at:      0     565    3111 
   Size:        565    2546
 
AvaI C'yCGr_G

Cuts at:      0    1715    3056    3111 
   Size:       1715    1341      55

  Fragments arranged by size: 

                1715    1341      55
 
AvrII C'CTAG_G

Cuts at:      0    1119    3111 
   Size:       1119    1992
 
BanI G'GyrC_C

Cuts at:      0     904    1929    2371    3111 
   Size:        904    1025     442     740

  Fragments arranged by size: 

                1025     904     740     442
 
BanII G_rGCy'C

Cuts at:      0     186     196     239     593     648     734    1053    1312 
   Size:        186      10      43     354      55      86     319     259

Cuts at:   1312    1704    1726    1850    2014    2204    2463    3089    3111 
   Size:        392      22     124     164     190     259     626      22

  Fragments arranged by size: 

                 626     392     354     319     259     259     190     186
                 164     124      86      55      43      22      22      10
            
 
BbeI G_GCGC'C

Cuts at:      0    1933    3111 
   Size:       1933    1178
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1757    1781    3111 
   Size:       1757      24    1330

  Fragments arranged by size: 

                1757    1330      24
 
BbvCI CC'TCA_GC

Cuts at:      0     553    1040    1838    1936    3111 
   Size:        553     487     798      98    1175

  Fragments arranged by size: 

                1175     798     553     487      98
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1816    1850    3111 
   Size:       1816      34    1261

  Fragments arranged by size: 

                1816    1261      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1867    3111 
   Size:       1867    1244
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1283    2791    3037    3111 
   Size:       1283    1508     246      74

  Fragments arranged by size: 

                1508    1283     246      74
 
BlpI GC'TnA_GC

Cuts at:      0     821    3111 
   Size:        821    2290
 
Bme1580I G_kGCm'C

Cuts at:      0     734    1850    1955    3111 
   Size:        734    1116     105    1156

  Fragments arranged by size: 

                1156    1116     734     105
 
BmgBI CAC'GTC

Cuts at:      0    1094    2547    3111 
   Size:       1094    1453     564

  Fragments arranged by size: 

                1453    1094     564
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     122    1971    2358    3111 
   Size:        122    1849     387     753

  Fragments arranged by size: 

                1849     753     387     122
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     692    1502    2148    3111 
   Size:        692     810     646     963

  Fragments arranged by size: 

                 963     810     692     646
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     442     484     499     544    1195    1315    1372    1582 
   Size:        442      42      15      45     651     120      57     210

Cuts at:   1582    1777    2121    2191    2770    2937    3111 
   Size:        195     344      70     579     167     174

  Fragments arranged by size: 

                 651     579     442     344     210     195     174     167
                 120      70      57      45      42      15
 
Bpu10I CC'TnA_GC

Cuts at:      0     553    1040    1477    1838    1936    3111 
   Size:        553     487     437     361      98    1175

  Fragments arranged by size: 

                1175     553     487     437     361      98
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     334    2188    3111 
   Size:        232     102    1854     923

  Fragments arranged by size: 

                1854     923     232     102
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2974    3111 
   Size:       2974     137
 
BsaHI Gr'CG_yC

Cuts at:      0     700     919    1930    3111 
   Size:        700     219    1011    1181

  Fragments arranged by size: 

                1181    1011     700     219
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     819     849    3111 
   Size:        819      30    2262

  Fragments arranged by size: 

                2262     819      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1663    1672    1675    1774    1777    1801    1804    1807 
   Size:       1663       9       3      99       3      24       3       3

Cuts at:   1807    1834    1855    1873    2124    2648    3001    3097    3111 
   Size:         27      21      18     251     524     353      96      14

  Fragments arranged by size: 

                1663     524     353     251      99      96      27      24
                  21      18      14       9       3       3       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0     160     638     955    1281    1590    1689    1898    2375 
   Size:        160     478     317     326     309      99     209     477

Cuts at:   2375    3111 
   Size:        736

  Fragments arranged by size: 

                 736     478     477     326     317     309     209     160
                  99
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58    2947    3111 
   Size:         58    2889     164

  Fragments arranged by size: 

                2889     164      58
 
BsiHKAI G_wGCw'C

Cuts at:      0     239    1258    1375    1744    2204    2408    3111 
   Size:        239    1019     117     369     460     204     703

  Fragments arranged by size: 

                1019     703     460     369     239     204     117
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     740    1100    2537    3111 
   Size:        740     360    1437     574

  Fragments arranged by size: 

                1437     740     574     360
 
Bsp1286I G_dGCh'C

Cuts at:      0     186     196     239     593     648     734    1053    1258 
   Size:        186      10      43     354      55      86     319     205

Cuts at:   1258    1312    1375    1704    1726    1744    1850    1955    2014 
   Size:         54      63     329      22      18     106     105      59

Cuts at:   2014    2204    2408    2463    3089    3111 
   Size:        190     204      55     626      22

  Fragments arranged by size: 

                 626     354     329     319     205     204     190     186
                 106     105      86      63      59      55      55      54
                  43      22      22      18      10
 
BspHI T'CATG_A

Cuts at:      0    1250    3111 
   Size:       1250    1861
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1908    2965    3111 
   Size:       1908    1057     146

  Fragments arranged by size: 

                1908    1057     146
 
BsrBI CCG'CTC

Cuts at:      0     686     825    3111 
   Size:        686     139    2286

  Fragments arranged by size: 

                2286     686     139
 
BsrDI GCAATG_nn'

Cuts at:      0     530    1379    2373    2585    3111 
   Size:        530     849     994     212     526

  Fragments arranged by size: 

                 994     849     530     526     212
 
BsrFI r'CCGG_y

Cuts at:      0     105    1240    2951    3075    3111 
   Size:        105    1135    1711     124      36

  Fragments arranged by size: 

                1711    1135     124     105      36
 
BssSI C'ACGA_G

Cuts at:      0    2593    3111 
   Size:       2593     518
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     225     264     366     393     489    1341    1914    3111 
   Size:        225      39     102      27      96     852     573    1197

  Fragments arranged by size: 

                1197     852     573     225     102      96      39      27
            
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     135     168    2262    2368    3111 
   Size:        135      33    2094     106     743

  Fragments arranged by size: 

                2094     743     135     106      33
 
BstYI r'GATC_y

Cuts at:      0    1598    2900    3111 
   Size:       1598    1302     211

  Fragments arranged by size: 

                1598    1302     211
 
Bsu36I CC'TnA_GG

Cuts at:      0    1646    3111 
   Size:       1646    1465
 
BtgI C'CryG_G

Cuts at:      0      61     134    1133    1314    1395    1462    2261    2361 
   Size:         61      73     999     181      81      67     799     100

Cuts at:   2361    3008    3089    3111 
   Size:        647      81      22

  Fragments arranged by size: 

                 999     799     647     181     100      81      81      73
                  67      61      22
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     969    2407    3111 
   Size:        969    1438     704

  Fragments arranged by size: 

                1438     969     704
 
BtsI GCAGTG_nn'

Cuts at:      0     208    1160    2162    2879    3111 
   Size:        208     952    1002     717     232

  Fragments arranged by size: 

                1002     952     717     232     208
 
DraIII CAC_nnn'GTG

Cuts at:      0    2117    3111 
   Size:       2117     994
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2026    3111 
   Size:       2026    1085
 
EaeI y'GGCC_r

Cuts at:      0    1430    1943    2694    2876    3111 
   Size:       1430     513     751     182     235

  Fragments arranged by size: 

                1430     751     513     235     182
 
EarI CTCTTCn'nnn_

Cuts at:      0     452     508     877    2002    2044    2462    3111 
   Size:        452      56     369    1125      42     418     649

  Fragments arranged by size: 

                1125     649     452     418     369      56      42
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2613    3111 
   Size:       2613     498
 
EcoICRI GAG'CTC

Cuts at:      0     237    2202    3111 
   Size:        237    1965     909

  Fragments arranged by size: 

                1965     909     237
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     442     484     499     544    1195    1315    1372    1582 
   Size:        442      42      15      45     651     120      57     210

Cuts at:   1582    1777    2121    2191    2486    2550    2770    2937    3111 
   Size:        195     344      70     295      64     220     167     174

  Fragments arranged by size: 

                 651     442     344     295     220     210     195     174
                 167     120      70      64      57      45      42      15
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1222    1759    2962    3111 
   Size:       1222     537    1203     149

  Fragments arranged by size: 

                1222    1203     537     149
 
EcoO109I rG'GnC_Cy

Cuts at:      0     731    1206    1518    1846    1962    2050    2334    3111 
   Size:        731     475     312     328     116      88     284     777

  Fragments arranged by size: 

                 777     731     475     328     312     284     116      88
            
 
EcoRI G'AATT_C

Cuts at:      0     565    3111 
   Size:        565    2546
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2055    3111 
   Size:       2055    1056
 
FspI TGC'GCA

Cuts at:      0    2149    3111 
   Size:       2149     962
 
FspAI rTGC'GCAy

Cuts at:      0    2149    3111 
   Size:       2149     962
 
HaeII r_GCGC'y

Cuts at:      0     247    1933    3008    3111 
   Size:        247    1686    1075     103

  Fragments arranged by size: 

                1686    1075     247     103
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      13      18      45      50     819     851    1023    1055 
   Size:         13       5      27       5     769      32     172      32

Cuts at:   1055    2204    2236    3111 
   Size:       1149      32     875

  Fragments arranged by size: 

                1149     875     769     172      32      32      32      27
                  13       5       5
 
HincII GTy'rAC

Cuts at:      0    1181    3111 
   Size:       1181    1930
 
KasI G'GCGC_C

Cuts at:      0    1929    3111 
   Size:       1929    1182
 
MscI TGG'CCA

Cuts at:      0    1432    1945    2696    2878    3111 
   Size:       1432     513     751     182     233

  Fragments arranged by size: 

                1432     751     513     233     182
 
MslI CAynn'nnrTG

Cuts at:      0      85    1030    1319    1384    1438    2098    2155    2260 
   Size:         85     945     289      65      54     660      57     105

Cuts at:   2260    2366    2426    3111 
   Size:        106      60     685

  Fragments arranged by size: 

                 945     685     660     289     106     105      85      65
                  60      57      54
 
MspA1I CmG'CkG

Cuts at:      0      63     264     336     432     438     450     474     489 
   Size:         63     201      72      96       6      12      24      15

Cuts at:    489    1464    1500    1530    1590    1905    2712    2941    3111 
   Size:        975      36      30      60     315     807     229     170

  Fragments arranged by size: 

                 975     807     315     229     201     170      96      72
                  63      60      36      30      24      15      12       6
            
 
NaeI GCC'GGC

Cuts at:      0    3077    3111 
   Size:       3077      34
 
NarI GG'CG_CC

Cuts at:      0    1930    3111 
   Size:       1930    1181
 
NcoI C'CATG_G

Cuts at:      0     134    2261    2361    3008    3089    3111 
   Size:        134    2127     100     647      81      22

  Fragments arranged by size: 

                2127     647     134     100      81      22
 
NgoMIV G'CCGG_C

Cuts at:      0    3075    3111 
   Size:       3075      36
 
NspI r_CATG'y

Cuts at:      0    1324    1342    1443    3111 
   Size:       1324      18     101    1668

  Fragments arranged by size: 

                1668    1324     101      18
 
PasI CC'CwG_GG

Cuts at:      0     694    1965    1996    3111 
   Size:        694    1271      31    1115

  Fragments arranged by size: 

                1271    1115     694      31
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     638    1965    2455    3111 
   Size:        638    1327     490     656

  Fragments arranged by size: 

                1327     656     638     490
 
PfoI T'CCnGG_A

Cuts at:      0     560    1559    3111 
   Size:        560     999    1552

  Fragments arranged by size: 

                1552     999     560
 
PpuMI rG'GwC_Cy

Cuts at:      0    2334    3111 
   Size:       2334     777
 
PshAI GACnn'nnGTC

Cuts at:      0    2652    3111 
   Size:       2652     459
 
PspOMI G'GGCC_C

Cuts at:      0     730    1846    3111 
   Size:        730    1116    1265

  Fragments arranged by size: 

                1265    1116     730
 
PstI C_TGCA'G

Cuts at:      0     230     269     341    1130    1337    1526    1910    2196 
   Size:        230      39      72     789     207     189     384     286

Cuts at:   2196    2828    3111 
   Size:        632     283

  Fragments arranged by size: 

                 789     632     384     286     283     230     207     189
                  72      39
 
PvuII CAG'CTG

Cuts at:      0     264     336     489    1530    1590    2712    3111 
   Size:        264      72     153    1041      60    1122     399

  Fragments arranged by size: 

                1122    1041     399     264     153      72      60
 
SacI G_AGCT'C

Cuts at:      0     239    2204    3111 
   Size:        239    1965     907

  Fragments arranged by size: 

                1965     907     239
 
SacII CC_GC'GG

Cuts at:      0      64    1465    3111 
   Size:         64    1401    1646

  Fragments arranged by size: 

                1646    1401      64
 
SalI G'TCGA_C

Cuts at:      0    1179    3111 
   Size:       1179    1932
 
SapI GCTCTTCn'nnn_

Cuts at:      0     452     508    2002    3111 
   Size:        452      56    1494    1109

  Fragments arranged by size: 

                1494    1109     452      56
 
SfcI C'TryA_G

Cuts at:      0     226     265     337    1126    1333    1522    1585    1906 
   Size:        226      39      72     789     207     189      63     321

Cuts at:   1906    2192    2285    2308    2824    3111 
   Size:        286      93      23     516     287

  Fragments arranged by size: 

                 789     516     321     287     286     226     207     189
                  93      72      63      39      23
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    3037    3111 
   Size:       3037      74
 
SfoI GGC'GCC

Cuts at:      0    1931    3111 
   Size:       1931    1180
 
SmaI CCC'GGG

Cuts at:      0    1717    3058    3111 
   Size:       1717    1341      53

  Fragments arranged by size: 

                1717    1341      53
 
SmlI C'TyrA_G

Cuts at:      0     247     349    2203    3111 
   Size:        247     102    1854     908

  Fragments arranged by size: 

                1854     908     247     102
 
SpeI A'CTAG_T

Cuts at:      0    1410    3111 
   Size:       1410    1701
 
SphI G_CATG'C

Cuts at:      0    1324    1443    3111 
   Size:       1324     119    1668

  Fragments arranged by size: 

                1668    1324     119
 
StyI C'CwwG_G

Cuts at:      0      47     134    1119    2261    2361    2831    3008    3089 
   Size:         47      87     985    1142     100     470     177      81

Cuts at:   3089    3111 
   Size:         22

  Fragments arranged by size: 

                1142     985     470     177     100      87      81      47
                  22
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1654    2389    2653    2998    3111 
   Size:       1654     735     264     345     113

  Fragments arranged by size: 

                1654     735     345     264     113
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1387    1419    2474    2506    2508    2540    3111 
   Size:       1387      32    1055      32       2      32     571

  Fragments arranged by size: 

                1387    1055     571      32      32      32       2
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2544    3111 
   Size:       2544     567
 
XmaI C'CCGG_G

Cuts at:      0    1715    3056    3111 
   Size:       1715    1341      55

  Fragments arranged by size: 

                1715    1341      55
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AlfI    AlwNI     ApaI     ApoI
     AvaI    AvrII     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    FspAI    HaeII    Hin4I   HincII
     KasI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PasI    PflMI     PfoI    PpuMI    PshAI   PspOMI     PstI
    PvuII     SacI    SacII     SalI     SapI     SfcI     SfiI     SfoI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TstI     XcmI
     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AloI    ApaLI     AscI     AseI    AsiSI     BaeI    BamHI     BclI
    BfrBI    BglII     BmtI    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI
     BsmI    BspEI    BsrGI   BssHII    BstBI   BstEII  BstZ17I     ClaI
    CspCI     DraI     EagI    EcoRV     FseI  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI
     PvuI    RsrII    SanDI     SbfI     ScaI    SexAI     SgfI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI
     XhoI     XmnI     ZraI
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