Restriction map for pF1KA0521
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
AscI        AseI        AsnI        Asp718I     AviII       
AvrII       BcgI        BciVI       BfrI        BlnI        
BsaBI       BseAI       BsiWI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
ClaI        CpoI        EcoO65I     EcoRI       EcoT22I     
FseI        FspI        HindIII     HpaI        KpnI        
KspI        MamI        MfeI        MroI        MslI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SapI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SspBI       SspI        
SwaI        XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII        3031  GACGTC               GACGT^C
AccI         2052  GTCGAC               GT^[AC][GT]AC
AflIII       2056  ACGCGT               A^C[GA][CT]GT
Alw44I        340  GTGCAC               G^TGCAC
AocI          921  CCTCAGG              CC^T[ACGT]AGG
ApaLI         340  GTGCAC               G^TGCAC
Asp700I      2034  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI        1934  GGATCC               G^GATCC
BanI         1872  GGCGCC               G^G[CT][GA]CC
BbeI         1872  GGCGCC               GGCGC^C
BbrPI         346  CACGTG               CAC^GTG
BbvCI         198  GCTGAGG              CCTCAGC(-5/-2)
BfaI         1456  CTAG                 C^TAG
BglII        1469  AGATCT               A^GATCT
BsaAI         346  CACGTG               [CT]AC^GT[GA]
BsaWI         527  ACCGGA               [AT]^CCGG[AT]
BsiEI        2648  CGGCCG               CG[GA][CT]^CG
BsmI          746  GCATTC               GAATGC(1/-1)
Bsu36I        921  CCTCAGG              CC^T[ACGT]AGG
DraI          108  TTTAAA               TTT^AAA
DraIII        343  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          886  GACGCCAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2648  CGGCCG               C^GGCCG
EclXI        2648  CGGCCG               C^GGCCG
Eco52I       2648  CGGCCG               C^GGCCG
Eco81I        921  CCTCAGG              CC^T[ACGT]AGG
HincII       2052  GTCGAC               GT[CT]^[GA]AC
HindII       2052  GTCGAC               GT[CT]^[GA]AC
KasI         1872  GGCGCC               G^GCGCC
MaeI         1456  CTAG                 C^TAG
MluI         2056  ACGCGT               A^CGCGT
NarI         1872  GGCGCC               GG^CGCC
PmaCI         346  CACGTG               CAC^GTG
PmlI          346  CACGTG               CAC^GTG
SalI         2052  GTCGAC               G^TCGAC
SfoI         1872  GGCGCC               GGC^GCC
XmnI         2034  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcsI          112  AAATTT               [GA]^AATT[CT]
              679  AAATTT              
Aor51HI      2165  AGCGCT               AGC^GCT
             2732  AGCGCT              
ApoI          112  AAATTT               [GA]^AATT[CT]
              679  AAATTT              
AspI         1941  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2852  GACACAGTC           
BclI          692  TGATCA               T^GATCA
             1220  TGATCA              
BglI           41  GCCCGGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1288  GCCTGGATGGC         
BsmBI         460  GAGACG               CGTCTC(1/5)
             2002  GAGACG              
BsrFI        2582  GCCGGC               [GA]^CCGG[CT]
             2988  GCCGGC              
BstXI        1904  CCACCGTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2669  CCACCAAGCTGG        
Cfr10I       2582  GCCGGC               [GA]^CCGG[CT]
             2988  GCCGGC              
EarI          433  CTCTTC               CTCTTC(1/4)
             1360  GAAGAG              
Eco47III     2165  AGCGCT               AGC^GCT
             2732  AGCGCT              
EcoRV         868  GATATC               GAT^ATC
             1066  GATATC              
FbaI          692  TGATCA               T^GATCA
             1220  TGATCA              
Ksp632I       433  CTCTTC               CTCTTC(1/4)
             1360  GAAGAG              
MseI          109  TTAA                 T^TAA
              116  TTAA                
NaeI         2582  GCCGGC               GCC^GGC
             2988  GCCGGC              
NgoMIV       2582  GCCGGC               G^CCGGC
             2988  GCCGGC              
PflFI        1941  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2852  GACACAGTC           
PflMI        2196  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2872  CCAGACCCTGG         
PpuMI          21  GGGTCCC              [GA]G^G[AT]CC[CT]
             1541  GGGACCC             
SbfI          390  CCTGCAGG             CCTGCA^GG
             1557  CCTGCAGG            
SphI         1028  GCATGC               GCATG^C
             2504  GCATGC              
Sse8387I      390  CCTGCAGG             CCTGCA^GG
             1557  CCTGCAGG            
Tru9I         109  TTAA                 T^TAA
              116  TTAA                
Tth111I      1941  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2852  GACACAGTC           
Van91I       2196  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2872  CCAGACCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AhdI        
AluI        AlwI        AlwNI       ApaI        AspEI       
AspHI       AvaI        AvaII       BalI        BanII       
BbsI        BbvI        BcnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrI        BssHII      BssKI       
BssSI       BstAPI      BstF5I      BstNI       BstUI       
BstYI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
Eam1105I    EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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