Restriction map for pF1KA0521
 (Circular) (Six-base) MAPSORT of: pF1KA0521.seq  Check: 3502  from: 1  to: 6158



pF1KA0521  6158 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1959    1959 
   Size:       6158
 
AatII G_ACGT'C

Cuts at:   3143    3143 
   Size:       6158
 
AccI GT'mk_AC

Cuts at:   2161    3196    2161 
   Size:       1035    5123
 
Acc65I G'GTAC_C

Cuts at:   3174    3174 
   Size:       6158
 
AclI AA'CG_TT

Cuts at:   5998    5998 
   Size:       6158
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    499     757    1078    1096    1440    2059    2890    3983    4415 
   Size:        258     321      18     344     619     831    1093     432

Cuts at:   4415    4768    5430     499 
   Size:        353     662    1227

  Fragments arranged by size: 

                1227    1093     831     662     619     432     353     344
                 321     258      18
 
AfeI AGC'GCT

Cuts at:   2275    2842    2275 
   Size:        567    5591
 
AflIII A'CryG_T

Cuts at:   2164    3510    5310    5591    2164 
   Size:       1346    1800     281    2731

  Fragments arranged by size: 

                2731    1800    1346     281
 
AhdI GACnn_n'nnGTC

Cuts at:    246     418    1059     246 
   Size:        172     641    5345

  Fragments arranged by size: 

                5345     641     172
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2138    2138 
   Size:       6158
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    499     531    2131    2163     499 
   Size:         32    1600      32    4494

  Fragments arranged by size: 

                4494    1600      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:   1722    2052    2310    2880    2883    2986    3463    4901    1722 
   Size:        330     258     570       3     103     477    1438    2979

  Fragments arranged by size: 

                2979    1438     570     477     330     258     103       3
            
 
ApaI G_GGCC'C

Cuts at:    498    1454    2602     498 
   Size:        956    1148    4054

  Fragments arranged by size: 

                4054    1148     956
 
ApaLI G'TGCA_C

Cuts at:    448    4996     448 
   Size:       4548    1610
 
ApoI r'AATT_y

Cuts at:    220     787    3162    5511    5767     220 
   Size:        567    2375    2349     256     611

  Fragments arranged by size: 

                2375    2349     611     567     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6158
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6158
 
AvaI C'yCGr_G

Cuts at:   1628    1886    2603    2653    3000    3178    1628 
   Size:        258     717      50     347     178    4608

  Fragments arranged by size: 

                4608     717     347     258     178      50
 
BamHI G'GATC_C

Cuts at:   2042    3183    2042 
   Size:       1141    5017
 
BanI G'GyrC_C

Cuts at:   1980    3174    3588    3836    3871    1980 
   Size:       1194     414     248      35    4267

  Fragments arranged by size: 

                4267    1194     414     248      35
 
BanII G_rGCy'C

Cuts at:    151     498    1088    1378    1454    1954    2602    2836    2854 
   Size:        347     590     290      76     500     648     234      18

Cuts at:   2854    2944    3007    3172    4202     151 
   Size:         90      63     165    1030    2107

  Fragments arranged by size: 

                2107    1030     648     590     500     347     290     234
                 165      90      76      63      18
 
BbeI G_GCGC'C

Cuts at:   1984    3592    3840    1984 
   Size:       1608     248    4302

  Fragments arranged by size: 

                4302    1608     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    950    1055    1109    1156    1619    1769    3143     950 
   Size:        105      54      47     463     150    1374    3965

  Fragments arranged by size: 

                3965    1374     463     150     105      54      47
 
BbvCI CC'TCA_GC

Cuts at:    307     307 
   Size:       6158
 
BciVI GTATCCnnnnn_n'

Cuts at:   4056    5112    6158    4056 
   Size:       1056    1046    4056

  Fragments arranged by size: 

                4056    1056    1046
 
BclI T'GATC_A

Cuts at:    800    1328    3678     800 
   Size:        528    2350    3280

  Fragments arranged by size: 

                3280    2350     528
 
BglI GCCn_nnn'nGGC

Cuts at:    155    1402     155 
   Size:       1247    4911
 
BglII A'GATC_T

Cuts at:   1577    3673    5710    1577 
   Size:       2096    2037    2025

  Fragments arranged by size: 

                2096    2037    2025
 
BlpI GC'TnA_GC

Cuts at:   2725    2853    2938    3270    2725 
   Size:        128      85     332    5613

  Fragments arranged by size: 

                5613     332     128      85
 
Bme1580I G_kGCm'C

Cuts at:    452     498    1454    2602    2714    3058    3783    3876    5000 
   Size:         46     956    1148     112     344     725      93    1124

Cuts at:   5000     452 
   Size:       1610

  Fragments arranged by size: 

                1610    1148    1124     956     725     344     112      93
                  46
 
BmrI ACTGGGnnnn_n'

Cuts at:    883    3370    3451    3786    5930     883 
   Size:       2487      81     335    2144    1111

  Fragments arranged by size: 

                2487    2144    1111     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2934    3054    4525    2934 
   Size:        120    1471    4567

  Fragments arranged by size: 

                4567    1471     120
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    375     474     586     640    1150    1606    2041    2203    2257 
   Size:         99     112      54     510     456     435     162      54

Cuts at:   2257    2332    2395    2416    2449    2500    2614     375 
   Size:         75      63      21      33      51     114    3919

  Fragments arranged by size: 

                3919     510     456     435     162     114     112      99
                  75      63      54      54      51      33      21
 
Bpu10I CC'TnA_GC

Cuts at:    307     307 
   Size:       6158
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    451     580    1060    1669    2482    3028    3334    4213    4927 
   Size:        129     480     609     813     546     306     879     714

Cuts at:   4927    5225     451 
   Size:        298    1384

  Fragments arranged by size: 

                1384     879     813     714     609     546     480     306
                 298     129
 
BsaI GGTCTCn'nnnn_

Cuts at:    120    1654    1795    5862     120 
   Size:       1534     141    4067     416

  Fragments arranged by size: 

                4067    1534     416     141
 
BsaAI yAC'GTr

Cuts at:    456    3511     456 
   Size:       3055    3103
 
BsaBI GATnn'nnATC

Cuts at:      6    3672    5709       6 
   Size:       3666    2037     455

  Fragments arranged by size: 

                3666    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    995    1981    3125    3140    3589    3837    4539    6035     995 
   Size:        986    1144      15     449     248     702    1496    1118

  Fragments arranged by size: 

                1496    1144    1118     986     702     449     248      15
            
 
BsaWI w'CCGG_w

Cuts at:    635    3347    3868    4546    4957    5104    5378     635 
   Size:       2712     521     678     411     147     274    1415

  Fragments arranged by size: 

                2712    1415     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    499     529    1360    1390    1544    1574    2131    2161    3010 
   Size:         30     831      30     154      30     557      30     849

Cuts at:   3010    3040     499 
   Size:         30    3617

  Fragments arranged by size: 

                3617     849     831     557     154      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    324     520     637    1324    1456    1459    2236     324 
   Size:        196     117     687     132       3     777    4246

  Fragments arranged by size: 

                4246     777     687     196     132     117       3
 
BseYI C'CCAG_C

Cuts at:    134    1622    1737    2057    2276    2447    2552    2572    2843 
   Size:       1488     115     320     219     171     105      20     271

Cuts at:   2843    3078    3583    4341    5006    5414     134 
   Size:        235     505     758     665     408     878

  Fragments arranged by size: 

                1488     878     758     665     505     408     320     271
                 235     219     171     115     105      20
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    865    1806    2782     865 
   Size:        941     976    4241

  Fragments arranged by size: 

                4241     976     941
 
BsiEI CG_ry'CG

Cuts at:    104    2759    3700    3746    4976     104 
   Size:       2655     941      46    1230    1286

  Fragments arranged by size: 

                2655    1286    1230     941      46
 
BsiHKAI G_wGCw'C

Cuts at:    452    1378    1954    2854    2917    3172    3950    4140    5000 
   Size:        926     576     900      63     255     778     190     860

Cuts at:   5000     452 
   Size:       1610

  Fragments arranged by size: 

                1610     926     900     860     778     576     255     190
                  63
 
BsmI GAATG_Cn'

Cuts at:    854     854 
   Size:       6158
 
BsmBI CGTCTCn'nnnn_

Cuts at:    562    2104     562 
   Size:       1542    4616
 
Bsp1286I G_dGCh'C

Cuts at:    151     452     498    1088    1378    1454    1954    2602    2714 
   Size:        301      46     590     290      76     500     648     112

Cuts at:   2714    2836    2854    2917    2944    3007    3058    3172    3783 
   Size:        122      18      63      27      63      51     114     611

Cuts at:   3783    3876    3950    4140    4202    5000     151 
   Size:         93      74     190      62     798    1309

  Fragments arranged by size: 

                1309     798     648     611     590     500     301     290
                 190     122     114     112      93      76      74      63
                  63      62      51      46      27      18
 
BspHI T'CATG_A

Cuts at:   4590    4590 
   Size:       6158
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    569    1625    1959    2183    3208    3422    3724    4105     569 
   Size:       1056     334     224    1025     214     302     381    2622

  Fragments arranged by size: 

                2622    1056    1025     381     334     302     224     214
            
 
BsrBI CCG'CTC

Cuts at:     50    1524    2136    2193    2370    2385    2433    2469    2933 
   Size:       1474     612      57     177      15      48      36     464

Cuts at:   2933    4450    4504    5988    6153      50 
   Size:       1517      54    1484     165      55

  Fragments arranged by size: 

                1517    1484    1474     612     464     177     165      57
                  55      54      48      36      15
 
BsrDI GCAATG_nn'

Cuts at:   4070    4070 
   Size:       6158
 
BsrFI r'CCGG_y

Cuts at:   2690    3096    4156    4337    2690 
   Size:        406    1060     181    4511

  Fragments arranged by size: 

                4511    1060     406     181
 
BssHII G'CGCG_C

Cuts at:   2226    2324    2522    2226 
   Size:         98     198    5862

  Fragments arranged by size: 

                5862     198      98
 
BssSI C'ACGA_G

Cuts at:    334    1371    1500    2263    4429    5137     334 
   Size:       1037     129     763    2166     708    1355

  Fragments arranged by size: 

                2166    1355    1037     763     708     129
 
BstAPI GCAn_nnn'nTGC

Cuts at:    456    1142    2880    2883    3535     456 
   Size:        686    1738       3     652    3079

  Fragments arranged by size: 

                3079    1738     686     652       3
 
BstBI TT'CG_AA

Cuts at:   4519    4519 
   Size:       6158
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2019    2784    2019 
   Size:        765    5393
 
BstYI r'GATC_y

Cuts at:      1     288    1577    2042    2771    3183    3650    3673    4008 
   Size:        287    1289     465     729     412     467      23     335

Cuts at:   4008    4254    4560    4572    4658    5710       1 
   Size:        246     306      12      86    1052     449

  Fragments arranged by size: 

                1289    1052     729     467     465     449     412     335
                 306     287     246      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1030    1030 
   Size:       6158
 
BtgI C'CryG_G

Cuts at:    365    1641    2456     365 
   Size:       1276     815    4067

  Fragments arranged by size: 

                4067    1276     815
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1333    2720    4108    4289    4452    1333 
   Size:       1387    1388     181     163    3039

  Fragments arranged by size: 

                3039    1388    1387     181     163
 
BtsI GCAGTG_nn'

Cuts at:    636    3091    3401     636 
   Size:       2455     310    3393

  Fragments arranged by size: 

                3393    2455     310
 
DraI TTT'AAA

Cuts at:    218    3157     218 
   Size:       2939    3219
 
DraIII CAC_nnn'GTG

Cuts at:    456     456 
   Size:       6158
 
DrdI GACnn_nn'nnGTC

Cuts at:   1000    3864    5208    1000 
   Size:       2864    1344    1950

  Fragments arranged by size: 

                2864    1950    1344
 
EaeI y'GGCC_r

Cuts at:    330     534     735    1301    1592    1691    1752    2543    2696 
   Size:        204     201     566     291      99      61     791     153

Cuts at:   2696    2756    3098    3743    3917    4308    4335    5680     330 
   Size:         60     342     645     174     391      27    1345     808

  Fragments arranged by size: 

                1345     808     791     645     566     391     342     291
                 204     201     174     153      99      61      60      27
            
 
EagI C'GGCC_G

Cuts at:   2756    3743    2756 
   Size:        987    5171
 
EarI CTCTTCn'nnn_

Cuts at:    547    1463    4181    4391     547 
   Size:        916    2718     210    2314

  Fragments arranged by size: 

                2718    2314     916     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1774    4366    5108    5254    5968    1774 
   Size:       2592     742     146     714    1964

  Fragments arranged by size: 

                2592    1964     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1376    1952    2852    3170    1376 
   Size:        576     900     318    4364

  Fragments arranged by size: 

                4364     900     576     318
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    375     474     499     586     640     757    1078    1096    1150 
   Size:         99      25      87      54     117     321      18      54

Cuts at:   1150    1440    1606    2041    2059    2203    2257    2332    2395 
   Size:        290     166     435      18     144      54      75      63

Cuts at:   2395    2416    2449    2500    2614    2890    3983    4415    4768 
   Size:         21      33      51     114     276    1093     432     353

Cuts at:   4768    5430     375 
   Size:        662    1103

  Fragments arranged by size: 

                1103    1093     662     435     432     353     321     290
                 276     166     144     117     114      99      87      75
                  63      54      54      54      51      33      25      21
                  18      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:    306     502     622     952     306 
   Size:        196     120     330    5512

  Fragments arranged by size: 

                5512     330     196     120
 
EcoO109I rG'GnC_Cy

Cuts at:    130     494     495    1450    1451    1650    1883    1900    2598 
   Size:        364       1     955       1     199     233      17     698

Cuts at:   2598    2599    3297     130 
   Size:          1     698    2991

  Fragments arranged by size: 

                2991     955     698     698     364     233     199      17
                   1       1       1
 
EcoRI G'AATT_C

Cuts at:   3162    3162 
   Size:       6158
 
EcoRV GAT'ATC

Cuts at:    978    1176     978 
   Size:        198    5960
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    439    1126    4177     439 
   Size:        687    3051    2420

  Fragments arranged by size: 

                3051    2420     687
 
FspI TGC'GCA

Cuts at:   3939    3939 
   Size:       6158
 
HaeII r_GCGC'y

Cuts at:    606    1029    1167    1337    1748    1984    2277    2292    2403 
   Size:        423     138     170     411     236     293      15     111

Cuts at:   2403    2409    2478    2652    2678    2732    2844    3116    3592 
   Size:          6      69     174      26      54     112     272     476

Cuts at:   3592    3840    5070    5841     606 
   Size:        248    1230     771     923

  Fragments arranged by size: 

                1230     923     771     476     423     411     293     272
                 248     236     174     170     138     112     111      69
                  54      26      15       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    102     134     232     264     307     339     404     436     967 
   Size:         32      98      32      43      32      65      32     531

Cuts at:    967     999    1045    1077    3010    3042    4000    4030    4032 
   Size:         32      46      32    1933      32     958      30       2

Cuts at:   4032    4062     102 
   Size:         30    2198

  Fragments arranged by size: 

                2198    1933     958     531      98      65      46      43
                  32      32      32      32      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   2162    3197    2162 
   Size:       1035    5123
 
KasI G'GCGC_C

Cuts at:   1980    3588    3836    1980 
   Size:       1608     248    4302

  Fragments arranged by size: 

                4302    1608     248
 
KpnI G_GTAC'C

Cuts at:   3178    3178 
   Size:       6158
 
MluI A'CGCG_T

Cuts at:   2164    5591    2164 
   Size:       3427    2731
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2006    4917    5101    2006 
   Size:       2911     184    3063

  Fragments arranged by size: 

                3063    2911     184
 
MscI TGG'CCA

Cuts at:    737    1303    2698    3919     737 
   Size:        566    1395    1221    2976

  Fragments arranged by size: 

                2976    1395    1221     566
 
MslI CAynn'nnrTG

Cuts at:   4147    4274    4147 
   Size:        127    6031
 
MspA1I CmG'CkG

Cuts at:    235    1116    1545    2040    2061    2118    2217    2256    2721 
   Size:        881     429     495      21      57      99      39     465

Cuts at:   2721    2880    3016    3269    3583    3943    4727    4972     235 
   Size:        159     136     253     314     360     784     245    1421

  Fragments arranged by size: 

                1421     881     784     495     465     429     360     314
                 253     245     159     136      99      57      39      21
            
 
NaeI GCC'GGC

Cuts at:   2692    3098    4339    2692 
   Size:        406    1241    4511

  Fragments arranged by size: 

                4511    1241     406
 
NarI GG'CG_CC

Cuts at:   1981    3589    3837    1981 
   Size:       1608     248    4302

  Fragments arranged by size: 

                4302    1608     248
 
NgoMIV G'CCGG_C

Cuts at:   2690    3096    4337    2690 
   Size:        406    1241    4511

  Fragments arranged by size: 

                4511    1241     406
 
NspI r_CATG'y

Cuts at:   1110    1140    2616    3211    3711    5314    1110 
   Size:         30    1476     595     500    1603    1954

  Fragments arranged by size: 

                1954    1603    1476     595     500      30
 
PasI CC'CwG_GG

Cuts at:    160     160 
   Size:       6158
 
PciI A'CATG_T

Cuts at:   5310    5310 
   Size:       6158
 
PflMI CCAn_nnn'nTGG

Cuts at:   2310    2986    2310 
   Size:        676    5482
 
PfoI T'CCnGG_A

Cuts at:    352    1508    2018    2129     352 
   Size:       1156     510     111    4381

  Fragments arranged by size: 

                4381    1156     510     111
 
PmeI GTTT'AAAC

Cuts at:   3157    3157 
   Size:       6158
 
PmlI CAC'GTG

Cuts at:    456     456 
   Size:       6158
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    499     531    2131    2163    2917    2949    4604    4636     499 
   Size:         32    1600      32     754      32    1655      32    2021

  Fragments arranged by size: 

                2021    1655    1600     754      32      32      32      32
            
 
PpuMI rG'GwC_Cy

Cuts at:    130    1650     130 
   Size:       1520    4638
 
PspOMI G'GGCC_C

Cuts at:    494    1450    2598     494 
   Size:        956    1148    4054

  Fragments arranged by size: 

                4054    1148     956
 
PstI C_TGCA'G

Cuts at:    503     515    1670    1732    2159    2249    2336    3205    3890 
   Size:         12    1155      62     427      90      87     869     685

Cuts at:   3890     503 
   Size:       2771

  Fragments arranged by size: 

                2771    1155     869     685     427      90      87      62
                  12
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6158
 
PvuII CAG'CTG

Cuts at:    235    1545    2061    2256    2721    2880    3016    3583    3943 
   Size:       1310     516     195     465     159     136     567     360

Cuts at:   3943     235 
   Size:       2450

  Fragments arranged by size: 

                2450    1310     567     516     465     360     195     159
                 136
 
RsrII CG'GwC_CG

Cuts at:   4353    4353 
   Size:       6158
 
SacI G_AGCT'C

Cuts at:   1378    1954    2854    3172    1378 
   Size:        576     900     318    4364

  Fragments arranged by size: 

                4364     900     576     318
 
SalI G'TCGA_C

Cuts at:   2160    3195    2160 
   Size:       1035    5123
 
SanDI GG'GwC_CC

Cuts at:    130    1650     130 
   Size:       1520    4638
 
SapI GCTCTTCn'nnn_

Cuts at:   4181    4391    4181 
   Size:        210    5948
 
SbfI CC_TGCA'GG

Cuts at:    503    1670    3205     503 
   Size:       1167    1535    3456

  Fragments arranged by size: 

                3456    1535    1167
 
ScaI AGT'ACT

Cuts at:   5675    5675 
   Size:       6158
 
SfcI C'TryA_G

Cuts at:     33     499     511    1666    1728    2155    2245    2332    3201 
   Size:        466      12    1155      62     427      90      87     869

Cuts at:   3201    3886    4854    5045      33 
   Size:        685     968     191    1146

  Fragments arranged by size: 

                1155    1146     968     869     685     466     427     191
                  90      87      62      12
 
SfoI GGC'GCC

Cuts at:   1982    3590    3838    1982 
   Size:       1608     248    4302

  Fragments arranged by size: 

                4302    1608     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6158
 
SmaI CCC'GGG

Cuts at:   3180    3180 
   Size:       6158
 
SmlI C'TyrA_G

Cuts at:    466     595    1075    1684    2497    3043    3313    4228    4942 
   Size:        129     480     609     813     546     270     915     714

Cuts at:   4942    5204     466 
   Size:        262    1420

  Fragments arranged by size: 

                1420     915     813     714     609     546     480     270
                 262     129
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6158
 
SphI G_CATG'C

Cuts at:   1140    2616    3211    3711    1140 
   Size:       1476     595     500    3587

  Fragments arranged by size: 

                3587    1476     595     500
 
StuI AGG'CCT

Cuts at:    958    1634    2413     958 
   Size:        676     779    4703

  Fragments arranged by size: 

                4703     779     676
 
StyI C'CwwG_G

Cuts at:    998    2798    3116    3292    3646     998 
   Size:       1800     318     176     354    3510

  Fragments arranged by size: 

                3510    1800     354     318     176
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1210    3741    3874    4333    4542    1210 
   Size:       2531     133     459     209    2826

  Fragments arranged by size: 

                2826    2531     459     209     133
 
TatI w'GTAC_w

Cuts at:    485    5673     485 
   Size:       5188     970
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1544    1576    5675    5707    1544 
   Size:         32    4099      32    1995

  Fragments arranged by size: 

                4099    1995      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2052    2963    3955    2052 
   Size:        911     992    4255

  Fragments arranged by size: 

                4255     992     911
 
XbaI T'CTAG_A

Cuts at:   3189    3189 
   Size:       6158
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3653    3653 
   Size:       6158
 
XmaI C'CCGG_G

Cuts at:   3178    3178 
   Size:       6158
 
XmnI GAAnn'nnTTC

Cuts at:   2146    2146 
   Size:       6158
 
ZraI GAC'GTC

Cuts at:   3141    3141 
   Size:       6158
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

    AflII     AgeI     AleI     AscI    AvrII     BaeI     BcgI    BfrBI
    BmgBI     BmtI    BsiWI    BspEI    BsrGI   BstEII  BstZ17I     ClaI
    CspCI     FseI    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI    PshAI     PsiI    PspXI
     PsrI    SacII    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     SwaI     XhoI
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