Restriction map for pF1KA0400
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AclI        AflII       AflIII      AgeI        AhdI        
ApaI        AscI        AseI        AsnI        Asp700I     
AspEI       AvaI        AviII       BciVI       BfrI        
BglI        BlpI        Bpu1102I    BsiEI       BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     CelII       ClaI        
CpoI        DrdI        EagI        Eam1105I    EclXI       
Eco52I      EcoO65I     EcoRV       FseI        FspI        
HpaI        KspI        MfeI        MluI        MunI        
NdeI        NheI        NotI        NruI        NspI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SwaI        XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII        1300  GACGTC               GACGT^C
Acc65I       1018  GGTACC               G^GTACC
AccIII       1378  TCCGGA               T^CCGGA
Alw44I       3001  GTGCAC               G^TGCAC
Aor51HI      2850  AGCGCT               AGC^GCT
ApaLI        3001  GTGCAC               G^TGCAC
Asp718I      1018  GGTACC               G^GTACC
AspI         1525  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        2806  CCTAGG               C^CTAGG
BamHI         953  GGATCC               G^GATCC
BbrPI         202  CACGTG               CAC^GTG
BbvCI        1519  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1551  CGACATGAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         2807  CTAG                 C^TAG
BglII          11  AGATCT               A^GATCT
BlnI         2806  CCTAGG               C^CTAGG
BsaAI         202  CACGTG               [CT]AC^GT[GA]
BsaBI        2959  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI           37  GAGACC               GGTCTC(1/5)
BseAI        1378  TCCGGA               T^CCGGA
BspEI        1378  TCCGGA               T^CCGGA
BssSI        1996  CACGAG               CACGAG(-5/-1)
BstAPI       2227  GCAAACCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI         1197  TTTAAA               TTT^AAA
DraIII       2406  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     2850  AGCGCT               AGC^GCT
EcoNI        2232  CCTTGCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI          25  GAATTC               G^AATTC
EcoT22I      1205  ATGCAT               ATGCA^T
HgaI         2556  GACGC                GACGC(5/10)
KpnI         1018  GGTACC               GGTAC^C
MaeI         2807  CTAG                 C^TAG
MamI         2959  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1378  TCCGGA               T^CCGGA
MslI         2953  CACATTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI         2447  GCCGGC               GCC^GGC
NgoMIV       2447  GCCGGC               G^CCGGC
NsiI         1205  ATGCAT               ATGCA^T
PflFI        1525  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         228  CCAGGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         202  CACGTG               CAC^GTG
PmlI          202  CACGTG               CAC^GTG
SacI         2884  GAGCTC               GAGCT^C
SapI          214  GAAGAGC              GCTCTTC(1/4)
SspI         1471  AATATT               AAT^ATT
StuI         1931  AGGCCT               AGG^CCT
Tth111I      1525  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        228  CCAGGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccI          744  GTCTAC               GT^[AC][GT]AC
             1810  GTAGAC              
AcsI           25  GAATTC               [GA]^AATT[CT]
              857  AAATTC              
AocI          892  CCTCAGG              CC^T[ACGT]AGG
             1078  CCTGAGG             
ApoI           25  GAATTC               [GA]^AATT[CT]
              857  AAATTC              
BbeI           57  GGCGCC               GGCGC^C
             1320  GGCGCC              
BclI          659  TGATCA               T^GATCA
             2909  TGATCA              
BcnI         2680  CCGGG                CC^[GC]GG
             2988  CCCGG               
BsaWI         140  ACCGGA               [AT]^CCGG[AT]
             1378  TCCGGA              
BsrBI         539  GAGCGG               CCGCTC(-3/-3)
              919  GAGCGG              
Bsu36I        892  CCTCAGG              CC^T[ACGT]AGG
             1078  CCTGAGG             
Eco81I        892  CCTCAGG              CC^T[ACGT]AGG
             1078  CCTGAGG             
FbaI          659  TGATCA               T^GATCA
             2909  TGATCA              
HincII        321  GTTGAC               GT[CT]^[GA]AC
              613  GTCAAC              
HindII        321  GTTGAC               GT[CT]^[GA]AC
              613  GTCAAC              
KasI           57  GGCGCC               G^GCGCC
             1320  GGCGCC              
NarI           57  GGCGCC               GG^CGCC
             1320  GGCGCC              
NciI         2680  CCGGG                CC^[GC]GG
             2988  CCCGG               
NcoI          510  CCATGG               C^CATGG
             1014  CCATGG              
PstI         2327  CTGCAG               CTGCA^G
             2785  CTGCAG              
SfoI           57  GGCGCC               GGC^GCC
             1320  GGCGCC              
XcmI         1324  CCAGATCCTACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2022  CCAAGCCTTATCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaII       BalI        
BanI        BanII       BbsI        BbvI        BmyI        
BpmI        BpuAI       BsaHI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BspMI       BsrDI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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