Restriction map for pF1KA0059
 (Linear) (Six-base) MAPSORT of: KIAA0059.seq  Check: 4081  from: 1  to: 2154



KIAA0059.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:54  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     199     706    2154 
   Size:        199     507    1448

  Fragments arranged by size: 

                1448     507     199
 
AatII G_ACGT'C

Cuts at:      0    1617    2154 
   Size:       1617     537
 
AccI GT'mk_AC

Cuts at:      0     947    2154 
   Size:        947    1207
 
Acc65I G'GTAC_C

Cuts at:      0    1133    2154 
   Size:       1133    1021
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2078    2154 
   Size:       2078      76
 
AflIII A'CryG_T

Cuts at:      0    1946    2154 
   Size:       1946     208
 
AlwNI CAG_nnn'CTG

Cuts at:      0     534    2154 
   Size:        534    1620
 
ApaLI G'TGCA_C

Cuts at:      0     446     743    2154 
   Size:        446     297    1411

  Fragments arranged by size: 

                1411     446     297
 
AseI AT'TA_AT

Cuts at:      0    1639    2154 
   Size:       1639     515
 
BanI G'GyrC_C

Cuts at:      0    1133    1482    2154 
   Size:       1133     349     672

  Fragments arranged by size: 

                1133     672     349
 
BanII G_rGCy'C

Cuts at:      0      29    1183    2154 
   Size:         29    1154     971

  Fragments arranged by size: 

                1154     971      29
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     847    2154 
   Size:        847    1307
 
BglII A'GATC_T

Cuts at:      0    1023    1573    2154 
   Size:       1023     550     581

  Fragments arranged by size: 

                1023     581     550
 
Bme1580I G_kGCm'C

Cuts at:      0     450     747    2154 
   Size:        450     297    1407

  Fragments arranged by size: 

                1407     450     297
 
BmgBI CAC'GTC

Cuts at:      0    1229    2154 
   Size:       1229     925
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     268    2154 
   Size:        268    1886
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     471     730     888    2059    2154 
   Size:        471     259     158    1171      95

  Fragments arranged by size: 

                1171     471     259     158      95
 
Bpu10I CC'TnA_GC

Cuts at:      0    1337    2154 
   Size:       1337     817
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     395    2154 
   Size:        395    1759
 
BsaHI Gr'CG_yC

Cuts at:      0    1614    2154 
   Size:       1614     540
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     392     422    1095    1125    2154 
   Size:        392      30     673      30    1029

  Fragments arranged by size: 

                1029     673     392      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1943    2154 
   Size:       1943     211
 
BseYI C'CCAG_C

Cuts at:      0     736    1241    1470    1492    2154 
   Size:        736     505     229      22     662

  Fragments arranged by size: 

                 736     662     505     229      22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     240    2154 
   Size:        240    1914
 
BsiEI CG_ry'CG

Cuts at:      0    1281    2154 
   Size:       1281     873
 
BsiHKAI G_wGCw'C

Cuts at:      0      29     450     630     747    2154 
   Size:         29     421     180     117    1407

  Fragments arranged by size: 

                1407     421     180     117      29
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1507    1605    2112    2154 
   Size:       1507      98     507      42

  Fragments arranged by size: 

                1507     507      98      42
 
Bsp1286I G_dGCh'C

Cuts at:      0      29     450     630     747    1183    2154 
   Size:         29     421     180     117     436     971

  Fragments arranged by size: 

                 971     436     421     180     117      29
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     199     706    2154 
   Size:        199     507    1448

  Fragments arranged by size: 

                1448     507     199
 
BsrBI CCG'CTC

Cuts at:      0    1235    2154 
   Size:       1235     919
 
BsrDI GCAATG_nn'

Cuts at:      0    1269    1919    2154 
   Size:       1269     650     235

  Fragments arranged by size: 

                1269     650     235
 
BsrFI r'CCGG_y

Cuts at:      0     499    1301    1814    2154 
   Size:        499     802     513     340

  Fragments arranged by size: 

                 802     513     499     340
 
BsrGI T'GTAC_A

Cuts at:      0    1199    2154 
   Size:       1199     955
 
BssSI C'ACGA_G

Cuts at:      0    1170    2154 
   Size:       1170     984
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     534     584     758    2154 
   Size:        534      50     174    1396

  Fragments arranged by size: 

                1396     534     174      50
 
BstEII G'GTnAC_C

Cuts at:      0    1986    2105    2154 
   Size:       1986     119      49

  Fragments arranged by size: 

                1986     119      49
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     124     301    1258    1528    1950    2154 
   Size:        124     177     957     270     422     204

  Fragments arranged by size: 

                 957     422     270     204     177     124
 
BstYI r'GATC_y

Cuts at:      0     956    1023    1573    2154 
   Size:        956      67     550     581

  Fragments arranged by size: 

                 956     581     550      67
 
Bsu36I CC'TnA_GG

Cuts at:      0      74    2154 
   Size:         74    2080
 
BtgI C'CryG_G

Cuts at:      0      47     294    2154 
   Size:         47     247    1860

  Fragments arranged by size: 

                1860     247      47
 
BtsI GCAGTG_nn'

Cuts at:      0     111     559    2154 
   Size:        111     448    1595

  Fragments arranged by size: 

                1595     448     111
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     183     218    2154 
   Size:        183      35    1936

  Fragments arranged by size: 

                1936     183      35
 
DraI TTT'AAA

Cuts at:      0     573    2154 
   Size:        573    1581
 
EaeI y'GGCC_r

Cuts at:      0    1278    1303    1899    2154 
   Size:       1278      25     596     255

  Fragments arranged by size: 

                1278     596     255      25
 
EagI C'GGCC_G

Cuts at:      0    1278    2154 
   Size:       1278     876
 
EarI CTCTTCn'nnn_

Cuts at:      0     504    1625    1676    2154 
   Size:        504    1121      51     478

  Fragments arranged by size: 

                1121     504     478      51
 
EcoICRI GAG'CTC

Cuts at:      0      27    2154 
   Size:         27    2127
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     471     730     888    2059    2078    2154 
   Size:        471     259     158    1171      19      76

  Fragments arranged by size: 

                1171     471     259     158      76      19
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2017    2154 
   Size:       2017     137
 
EcoO109I rG'GnC_Cy

Cuts at:      0      78     594    1341    1555    2154 
   Size:         78     516     747     214     599

  Fragments arranged by size: 

                 747     599     516     214      78
 
EcoRV GAT'ATC

Cuts at:      0     516    1449    2154 
   Size:        516     933     705

  Fragments arranged by size: 

                 933     705     516
 
HaeII r_GCGC'y

Cuts at:      0     387     535     544    2046    2154 
   Size:        387     148       9    1502     108

  Fragments arranged by size: 

                1502     387     148     108       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     336     368     392     424    1095    1127    1146    1178 
   Size:        336      32      24      32     671      32      19      32

Cuts at:   1178    1655    1687    2154 
   Size:        477      32     467

  Fragments arranged by size: 

                 671     477     467     336      32      32      32      32
                  32      24      19
 
HincII GTy'rAC

Cuts at:      0     439    2154 
   Size:        439    1715
 
HindIII A'AGCT_T

Cuts at:      0    1645    2154 
   Size:       1645     509
 
KpnI G_GTAC'C

Cuts at:      0    1137    2154 
   Size:       1137    1017
 
MfeI C'AATT_G

Cuts at:      0     357     492    2154 
   Size:        357     135    1662

  Fragments arranged by size: 

                1662     357     135
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1246    1351    1931    2154 
   Size:       1246     105     580     223

  Fragments arranged by size: 

                1246     580     223     105
 
MscI TGG'CCA

Cuts at:      0    1901    2154 
   Size:       1901     253
 
MslI CAynn'nnrTG

Cuts at:      0     122     299    1355    2154 
   Size:        122     177    1056     799

  Fragments arranged by size: 

                1056     799     177     122
 
MspA1I CmG'CkG

Cuts at:      0     273     740    1277    1865    2154 
   Size:        273     467     537     588     289

  Fragments arranged by size: 

                 588     537     467     289     273
 
NaeI GCC'GGC

Cuts at:      0     501    2154 
   Size:        501    1653
 
NcoI C'CATG_G

Cuts at:      0      47     294    2154 
   Size:         47     247    1860

  Fragments arranged by size: 

                1860     247      47
 
NdeI CA'TA_TG

Cuts at:      0    1971    2154 
   Size:       1971     183
 
NgoMIV G'CCGG_C

Cuts at:      0     499    2154 
   Size:        499    1655
 
NspI r_CATG'y

Cuts at:      0    1414    1908    1950    2154 
   Size:       1414     494      42     204

  Fragments arranged by size: 

                1414     494     204      42
 
PciI A'CATG_T

Cuts at:      0    1946    2154 
   Size:       1946     208
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     812    2154 
   Size:        812    1342
 
PfoI T'CCnGG_A

Cuts at:      0     707     959    2154 
   Size:        707     252    1195

  Fragments arranged by size: 

                1195     707     252
 
PpuMI rG'GwC_Cy

Cuts at:      0      78     594    1555    2154 
   Size:         78     516     961     599

  Fragments arranged by size: 

                 961     599     516      78
 
PvuII CAG'CTG

Cuts at:      0     740    2154 
   Size:        740    1414
 
SacI G_AGCT'C

Cuts at:      0      29    2154 
   Size:         29    2125
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1625    2154 
   Size:       1625     529
 
SexAI A'CCwGG_T

Cuts at:      0     921    2154 
   Size:        921    1233
 
SphI G_CATG'C

Cuts at:      0    1414    2154 
   Size:       1414     740
 
StuI AGG'CCT

Cuts at:      0    1658    2154 
   Size:       1658     496
 
StyI C'CwwG_G

Cuts at:      0      11      47     294    1086    1418    2154 
   Size:         11      36     247     792     332     736

  Fragments arranged by size: 

                 792     736     332     247      36      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     104     419     859    1154    1410    1940    2154 
   Size:        104     315     440     295     256     530     214

  Fragments arranged by size: 

                 530     440     315     295     256     214     104
 
TatI w'GTAC_w

Cuts at:      0     370     608    1199    2154 
   Size:        370     238     591     955

  Fragments arranged by size: 

                 955     591     370     238
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     538     570    2154 
   Size:        538      32    1584

  Fragments arranged by size: 

                1584     538      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     406    1107    2154 
   Size:        406     701    1047

  Fragments arranged by size: 

                1047     701     406
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     658    2154 
   Size:        658    1496
 
XmnI GAAnn'nnTTC

Cuts at:      0     149    2063    2154 
   Size:        149    1914      91

  Fragments arranged by size: 

                1914     149      91
 
ZraI GAC'GTC

Cuts at:      0    1615    2154 
   Size:       1615     539
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI   AflIII    AlwNI    ApaLI
     AseI     BanI    BanII     BbsI    BglII Bme1580I    BmgBI     BplI
     BpmI   Bpu10I     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI
     BtsI    CspCI     DraI     EaeI     EagI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRV    HaeII    Hin4I   HincII  HindIII     KpnI
     MfeI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI     NdeI
   NgoMIV     NspI     PciI    PflMI     PfoI    PpuMI    PvuII     SacI
     SapI    SexAI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AclI     AfeI    AflII     AgeI     AhdI     AleI     AlfI     AloI
     ApaI     ApoI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
     BmrI     BmtI    BpuEI    BsaAI    BsaBI    BsaWI    BsiWI     BsmI
    BspEI    BspHI   BssHII    BstBI  BstZ17I    BtgZI     ClaI   DraIII
     DrdI     EciI    EcoRI     FalI     FseI     FspI    FspAI     HpaI
     KasI     MluI     NarI     NheI     NotI     NruI     NsiI     PacI
     PasI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SrfI     SspI     SwaI     XbaI     XhoI     XmaI
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