Restriction map for pF1KA0044
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# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AgeI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspI        AviII       
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BciVI       BglI        BglII       BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiWI       
BsmBI       BsmFI       BsmI        BspDI       BspEI       
BspMI       BsrDI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstUI       
BstXI       BstZ17I     CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DrdI        Eco47III    
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FseI        FspI        HaeII       HhaI        
Hin1I       HinP1I      KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    StuI        SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccI          754  GTCTAC               GT^[AC][GT]AC
AclI          345  AACGTT               AA^CGTT
AflII         393  CTTAAG               C^TTAAG
AflIII        275  ACATGT               A^C[GA][CT]GT
AhdI          855  GACTCACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1351  CCTCAGG              CC^T[ACGT]AGG
AseI          664  ATTAAT               AT^TAAT
AsnI          664  ATTAAT               AT^TAAT
Asp700I      1152  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         855  GACTCACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          497  CTCGGG               C^[CT]CG[GA]G
AvrII         549  CCTAGG               C^CTAGG
BcgI         1061  CGAAGATTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          327  CCCGG                CC^[GC]GG
BfrI          393  CTTAAG               C^TTAAG
BlnI          549  CCTAGG               C^CTAGG
BsgI          784  GTGCAG               GTGCAG(16/14)
BsiEI        1419  CGGCCG               CG[GA][CT]^CG
BsoBI         497  CTCGGG               C^[CT]CG[GA]G
Bsp1407I     1192  TGTACA               T^GTACA
BspHI        1034  TCATGA               T^CATGA
BspLU11I      275  ACATGT               A^CATGT
BsrBI         314  GAGCGG               CCGCTC(-3/-3)
BsrGI        1192  TGTACA               T^GTACA
BstBI         281  TTCGAA               TT^CGAA
Bsu36I       1351  CCTCAGG              CC^T[ACGT]AGG
DraII        1355  AGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI           41  CCGTGG               C^C[GA][CT]GG
EagI         1419  CGGCCG               C^GGCCG
Eam1105I      855  GACTCACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1419  CGGCCG               C^GGCCG
Eco52I       1419  CGGCCG               C^GGCCG
Eco81I       1351  CCTCAGG              CC^T[ACGT]AGG
EcoO109I     1355  AGGACCC              [GA]G^G[ACGT]CC[CT]
HgaI          119  GCGTC                GACGC(5/10)
HincII        271  GTTAAC               GT[CT]^[GA]AC
HindII        271  GTTAAC               GT[CT]^[GA]AC
HindIII        88  AAGCTT               A^AGCTT
HpaI          271  GTTAAC               GTT^AAC
MspA1I        766  CAGCTG               C[AC]G^C[GT]G
NciI          327  CCCGG                CC^[GC]GG
NdeI          259  CATATG               CA^TATG
NspI          275  ACATGT               [GA]CATG^[CT]
NspV          281  TTCGAA               TT^CGAA
PflMI          36  CCAGCCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1355  AGGACCC              [GA]G^G[AT]CC[CT]
PshBI         664  ATTAAT               AT^TAAT
Psp1406I      345  AACGTT               AA^CGTT
PstI         1389  CTGCAG               CTGCA^G
PvuII         766  CAGCTG               CAG^CTG
RcaI         1034  TCATGA               T^CATGA
SfuI          281  TTCGAA               TT^CGAA
SspBI        1192  TGTACA               T^GTACA
SspI          648  AATATT               AAT^ATT
Van91I         36  CCAGCCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1367  CCAAGAAAGCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1152  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AfaI         1092  GTAC                 GT^AC
             1193  GTAC                
BalI          847  TGGCCA               TGG^CCA
              962  TGGCCA              
BanII         475  GAGCTC               G[GA]GC[CT]^C
             1004  GAGCTC              
BfaI          550  CTAG                 C^TAG
             1276  CTAG                
BpmI          410  CTCCAG               CTGGAG(16/14)
              975  CTCCAG              
BsmAI         408  GTCTC                GTCTC(1/5)
              973  GTCTC               
BstF5I        760  CATCC                GGATG(2/0)
                9  GGATG               
BstYI         492  AGATCC               [GA]^GATC[CT]
             1305  AGATCC              
DraIII        146  CACTAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
              733  CACAAAGTG           
EcoT14I       549  CCTAGG               C^C[AT][AT]GG
             1376  CCTTGG              
FokI          760  CATCC                GGATG(9/13)
                9  GGATG               
HapII         328  CCGG                 C^CGG
              954  CCGG                
HpaII         328  CCGG                 C^CGG
              954  CCGG                
HphI          825  GGTGA                GGTGA(8/7)
              209  TCACC               
MaeI          550  CTAG                 C^TAG
             1276  CTAG                
MaeII         108  ACGT                 A^CGT
              346  ACGT                
MflI          492  AGATCC               [GA]^GATC[CT]
             1305  AGATCC              
MluNI         847  TGGCCA               TGG^CCA
              962  TGGCCA              
MscI          847  TGGCCA               TGG^CCA
              962  TGGCCA              
MslI          619  CATCATAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1161  CATGGAGATG          
MspI          328  CCGG                 C^CGG
              954  CCGG                
PleI          855  GACTC                GAGTC(4/5)
              406  GAGTC               
RsaI         1092  GTAC                 GT^AC
             1193  GTAC                
SacI          475  GAGCTC               GAGCT^C
             1004  GAGCTC              
SapI          691  GAAGAGC              GCTCTTC(1/4)
             1155  GCTCTTC             
SfaNI          10  GATGC                GCATC(5/9)
              618  GCATC               
SfcI          370  CTACAG               C^T[GA][CT]AG
             1389  CTGCAG              
SmlI          393  CTTAAG               C^T[CT][GA]AG
              555  CTTGAG              
StyI          549  CCTAGG               C^C[AT][AT]GG
             1376  CCTTGG              
TaqI           60  TCGA                 T^CGA
              282  TCGA                
TfiI          533  GAATC                G^A[AT]TC
             1065  GATTC               
Tsp45I        152  GTGAC                ^GT[GC]AC
             1049  GTGAC               
TthHB8I        60  TCGA                 T^CGA
              282  TCGA                
XhoII         492  AGATCC               [GA]^GATC[CT]
             1305  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
AspHI       AvaII       BbvI        BclI        BmyI        
BsaJI       BsiHKAI     BsiYI       BslI        Bsp1286I    
BsrI        BssKI       BstNI       Cac8I       Cfr13I      
DdeI        DpnII       EaeI        EarI        EcoRII      
FbaI        Fnu4HI      HaeIII      HinfI       ItaI        
Ksp632I     MaeIII      MboI        MboII       MnlI        
MseI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       Tru9I       
TseI        Tsp509I     TspRI       
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