Restriction map for pF1KA0044
 (Linear) (Six-base) MAPSORT of: KIAA0044.seq  Check: 594  from: 1  to: 1425



KIAA0044.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:54  ..

AccI GT'mk_AC

Cuts at:      0     755    1425 
   Size:        755     670
 
AclI AA'CG_TT

Cuts at:      0     346    1425 
   Size:        346    1079
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1171    1425 
   Size:       1171     254
 
AflII C'TTAA_G

Cuts at:      0     393    1425 
   Size:        393    1032
 
AflIII A'CryG_T

Cuts at:      0     275    1425 
   Size:        275    1150
 
AhdI GACnn_n'nnGTC

Cuts at:      0     860    1425 
   Size:        860     565
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     935     967    1425 
   Size:        935      32     458

  Fragments arranged by size: 

                 935     458      32
 
ApoI r'AATT_y

Cuts at:      0     319     385     544     925    1425 
   Size:        319      66     159     381     500

  Fragments arranged by size: 

                 500     381     319     159      66
 
AseI AT'TA_AT

Cuts at:      0     665    1425 
   Size:        665     760
 
AvaI C'yCGr_G

Cuts at:      0     497    1425 
   Size:        497     928
 
AvrII C'CTAG_G

Cuts at:      0     549    1425 
   Size:        549     876
 
BanII G_rGCy'C

Cuts at:      0     479    1008    1425 
   Size:        479     529     417

  Fragments arranged by size: 

                 529     479     417
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1050    1084    1425 
   Size:       1050      34     341

  Fragments arranged by size: 

                1050     341      34
 
BclI T'GATC_A

Cuts at:      0      78     227     447    1425 
   Size:         78     149     220     978

  Fragments arranged by size: 

                 978     220     149      78
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1425 
   Size:       1425
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     960    1425 
   Size:        395     565     465

  Fragments arranged by size: 

                 565     465     395
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     576    1425 
   Size:        576     849
 
BseYI C'CCAG_C

Cuts at:      0     764    1425 
   Size:        764     661
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     805    1425 
   Size:        805     620
 
BsiEI CG_ry'CG

Cuts at:      0    1422    1425 
   Size:       1422       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     479     698    1008    1425 
   Size:        479     219     310     417

  Fragments arranged by size: 

                 479     417     310     219
 
Bsp1286I G_dGCh'C

Cuts at:      0     479     698    1008    1425 
   Size:        479     219     310     417

  Fragments arranged by size: 

                 479     417     310     219
 
BspHI T'CATG_A

Cuts at:      0    1034    1425 
   Size:       1034     391
 
BsrBI CCG'CTC

Cuts at:      0     316    1425 
   Size:        316    1109
 
BsrGI T'GTAC_A

Cuts at:      0    1192    1425 
   Size:       1192     233
 
BstBI TT'CG_AA

Cuts at:      0     282    1425 
   Size:        282    1143
 
BstYI r'GATC_y

Cuts at:      0     492    1305    1425 
   Size:        492     813     120

  Fragments arranged by size: 

                 813     492     120
 
Bsu36I CC'TnA_GG

Cuts at:      0    1352    1425 
   Size:       1352      73
 
BtgI C'CryG_G

Cuts at:      0      41    1425 
   Size:         41    1384
 
BtsI GCAGTG_nn'

Cuts at:      0    1386    1425 
   Size:       1386      39
 
DraIII CAC_nnn'GTG

Cuts at:      0     151     738    1425 
   Size:        151     587     687

  Fragments arranged by size: 

                 687     587     151
 
EaeI y'GGCC_r

Cuts at:      0      14     847     962    1419    1425 
   Size:         14     833     115     457       6

  Fragments arranged by size: 

                 833     457     115      14       6
 
EagI C'GGCC_G

Cuts at:      0    1419    1425 
   Size:       1419       6
 
EarI CTCTTCn'nnn_

Cuts at:      0     686     890     955    1162    1425 
   Size:        686     204      65     207     263

  Fragments arranged by size: 

                 686     263     207     204      65
 
EcoICRI GAG'CTC

Cuts at:      0     477    1006    1425 
   Size:        477     529     419

  Fragments arranged by size: 

                 529     477     419
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     960    1171    1425 
   Size:        395     565     211     254

  Fragments arranged by size: 

                 565     395     254     211
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1356    1425 
   Size:       1356      69
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     106     723    1392    1425 
   Size:        106     617     669      33

  Fragments arranged by size: 

                 669     617     106      33
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     551     583     846     878    1425 
   Size:        551      32     263      32     547

  Fragments arranged by size: 

                 551     547     263      32      32
 
HincII GTy'rAC

Cuts at:      0     273    1425 
   Size:        273    1152
 
HindIII A'AGCT_T

Cuts at:      0      88    1425 
   Size:         88    1337
 
HpaI GTT'AAC

Cuts at:      0     273    1425 
   Size:        273    1152
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     444    1425 
   Size:        444     981
 
MscI TGG'CCA

Cuts at:      0     849     964    1425 
   Size:        849     115     461

  Fragments arranged by size: 

                 849     461     115
 
MslI CAynn'nnrTG

Cuts at:      0     623    1165    1425 
   Size:        623     542     260

  Fragments arranged by size: 

                 623     542     260
 
MspA1I CmG'CkG

Cuts at:      0     768    1425 
   Size:        768     657
 
NdeI CA'TA_TG

Cuts at:      0     260    1425 
   Size:        260    1165
 
NspI r_CATG'y

Cuts at:      0     279    1425 
   Size:        279    1146
 
PciI A'CATG_T

Cuts at:      0     275    1425 
   Size:        275    1150
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      42    1425 
   Size:         42    1383
 
PpuMI rG'GwC_Cy

Cuts at:      0    1356    1425 
   Size:       1356      69
 
PstI C_TGCA'G

Cuts at:      0    1393    1425 
   Size:       1393      32
 
PvuII CAG'CTG

Cuts at:      0     768    1425 
   Size:        768     657
 
SacI G_AGCT'C

Cuts at:      0     479    1008    1425 
   Size:        479     529     417

  Fragments arranged by size: 

                 529     479     417
 
SapI GCTCTTCn'nnn_

Cuts at:      0     686    1162    1425 
   Size:        686     476     263

  Fragments arranged by size: 

                 686     476     263
 
SfcI C'TryA_G

Cuts at:      0     370    1389    1425 
   Size:        370    1019      36

  Fragments arranged by size: 

                1019     370      36
 
SmlI C'TyrA_G

Cuts at:      0     393     555    1425 
   Size:        393     162     870

  Fragments arranged by size: 

                 870     393     162
 
SspI AAT'ATT

Cuts at:      0     650    1425 
   Size:        650     775
 
StyI C'CwwG_G

Cuts at:      0     549    1376    1425 
   Size:        549     827      49

  Fragments arranged by size: 

                 827     549      49
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     226    1425 
   Size:        226    1199
 
TatI w'GTAC_w

Cuts at:      0    1192    1425 
   Size:       1192     233
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1374    1425 
   Size:       1374      51
 
XmnI GAAnn'nnTTC

Cuts at:      0    1156    1425 
   Size:       1156     269
 

 Enzymes that do cut:

     AccI     AclI     AcuI    AflII   AflIII     AhdI     AloI     ApoI
     AseI     AvaI    AvrII    BanII     BcgI     BclI     BplI     BpmI
    BpuEI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BsrBI
    BsrGI    BstBI    BstYI   Bsu36I     BtgI     BtsI   DraIII     EaeI
     EagI     EarI  EcoICRI  Eco57MI EcoO109I     FalI    Hin4I   HincII
  HindIII     HpaI     MmeI     MscI     MslI   MspA1I     NdeI     NspI
     PciI    PflMI    PpuMI     PstI    PvuII     SacI     SapI     SfcI
     SmlI     SspI     StyI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI     AgeI     AleI     AlfI    AlwNI
     ApaI    ApaLI     AscI    AsiSI     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI   BstEII    BstXI  BstZ17I
    BtgZI     ClaI    CspCI     DraI     DrdI     EciI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TstI
  Tth111I     XbaI     XhoI     XmaI     ZraI
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