Restriction map for pF1KA0011
 (Linear) (Six-base) MAPSORT of: KIAA0011.seq  Check: 2181  from: 1  to: 2733



KIAA0011.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:54  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1614    2733 
   Size:       1614    1119
 
AatII G_ACGT'C

Cuts at:      0    1935    2733 
   Size:       1935     798
 
AccI GT'mk_AC

Cuts at:      0      68    1536    2733 
   Size:         68    1468    1197

  Fragments arranged by size: 

                1468    1197      68
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     239     481     568     617     782     833    1118 
   Size:        101     138     242      87      49     165      51     285

Cuts at:   1118    2318    2733 
   Size:       1200     415

  Fragments arranged by size: 

                1200     415     285     242     165     138     101      87
                  51      49
 
AfeI AGC'GCT

Cuts at:      0    1156    1963    2733 
   Size:       1156     807     770

  Fragments arranged by size: 

                1156     807     770
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1405    1437    2733 
   Size:       1405      32    1296

  Fragments arranged by size: 

                1405    1296      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     591    2733 
   Size:        591    2142
 
ApaI G_GGCC'C

Cuts at:      0     646     787    1371    2733 
   Size:        646     141     584    1362

  Fragments arranged by size: 

                1362     646     584     141
 
ApoI r'AATT_y

Cuts at:      0    1915    2733 
   Size:       1915     818
 
AvrII C'CTAG_G

Cuts at:      0     280    2733 
   Size:        280    2453
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     562     595    2733 
   Size:        562      33    2138

  Fragments arranged by size: 

                2138     562      33
 
BamHI G'GATC_C

Cuts at:      0    2137    2733 
   Size:       2137     596
 
BanI G'GyrC_C

Cuts at:      0    1105    1205    1453    2116    2733 
   Size:       1105     100     248     663     617

  Fragments arranged by size: 

                1105     663     617     248     100
 
BanII G_rGCy'C

Cuts at:      0     605     646     787     799    1197    1371    2186    2649 
   Size:        605      41     141      12     398     174     815     463

Cuts at:   2649    2733 
   Size:         84

  Fragments arranged by size: 

                 815     605     463     398     174     141      84      41
                  12
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     743    2733 
   Size:        743    1990
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1214    1248    2733 
   Size:       1214      34    1485

  Fragments arranged by size: 

                1485    1214      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1772    2733 
   Size:       1772     961
 
BclI T'GATC_A

Cuts at:      0     192    2733 
   Size:        192    2541
 
BglI GCCn_nnn'nGGC

Cuts at:      0     568    1558    1695    2733 
   Size:        568     990     137    1038

  Fragments arranged by size: 

                1038     990     568     137
 
BglII A'GATC_T

Cuts at:      0     246    2271    2733 
   Size:        246    2025     462

  Fragments arranged by size: 

                2025     462     246
 
BlpI GC'TnA_GC

Cuts at:      0    1562    2733 
   Size:       1562    1171
 
Bme1580I G_kGCm'C

Cuts at:      0     646     787     824    1210    1371    2733 
   Size:        646     141      37     386     161    1362

  Fragments arranged by size: 

                1362     646     386     161     141      37
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     981    1204    1424    2626    2733 
   Size:        981     223     220    1202     107

  Fragments arranged by size: 

                1202     981     223     220     107
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     224    2733 
   Size:        224    2509
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     194     213     733    1239    1300    2521    2733 
   Size:        194      19     520     506      61    1221     212

  Fragments arranged by size: 

                1221     520     506     212     194      61      19
 
Bpu10I CC'TnA_GC

Cuts at:      0     809    1672    2018    2733 
   Size:        809     863     346     715

  Fragments arranged by size: 

                 863     809     715     346
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1048    1402    1989    2733 
   Size:       1048     354     587     744

  Fragments arranged by size: 

                1048     744     587     354
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     276     385     520    1495    2733 
   Size:        276     109     135     975    1238

  Fragments arranged by size: 

                1238     975     276     135     109
 
BsaHI Gr'CG_yC

Cuts at:      0    1932    2733 
   Size:       1932     801
 
BsaWI w'CCGG_w

Cuts at:      0    1545    1904    2733 
   Size:       1545     359     829

  Fragments arranged by size: 

                1545     829     359
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     698     728    1045    1075    2524    2554    2733 
   Size:        698      30     317      30    1449      30     179

  Fragments arranged by size: 

                1449     698     317     179      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     213    1069    1460    2096    2733 
   Size:        213     856     391     636     637

  Fragments arranged by size: 

                 856     637     636     391     213
 
BseYI C'CCAG_C

Cuts at:      0    1157    2733 
   Size:       1157    1576
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152    2733 
   Size:        152    2581
 
BsiHKAI G_wGCw'C

Cuts at:      0     605     726     799    2186    2733 
   Size:        605     121      73    1387     547

  Fragments arranged by size: 

                1387     605     547     121      73
 
BsmI GAATG_Cn'

Cuts at:      0    2469    2733 
   Size:       2469     264
 
Bsp1286I G_dGCh'C

Cuts at:      0     605     646     726     787     799     824    1197    1210 
   Size:        605      41      80      61      12      25     373      13

Cuts at:   1210    1371    2186    2649    2733 
   Size:        161     815     463      84

  Fragments arranged by size: 

                 815     605     463     373     161      84      80      61
                  41      25      13      12
 
BspEI T'CCGG_A

Cuts at:      0    1545    2733 
   Size:       1545    1188
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      16    1052    1614    2733 
   Size:         16    1036     562    1119

  Fragments arranged by size: 

                1119    1036     562      16
 
BsrBI CCG'CTC

Cuts at:      0    1036    1188    2733 
   Size:       1036     152    1545

  Fragments arranged by size: 

                1545    1036     152
 
BsrDI GCAATG_nn'

Cuts at:      0    2186    2733 
   Size:       2186     547
 
BsrFI r'CCGG_y

Cuts at:      0      40    2733 
   Size:         40    2693
 
BsrGI T'GTAC_A

Cuts at:      0    1077    2733 
   Size:       1077    1656
 
BssSI C'ACGA_G

Cuts at:      0     836    2733 
   Size:        836    1897
 
BstBI TT'CG_AA

Cuts at:      0    2583    2733 
   Size:       2583     150
 
BstEII G'GTnAC_C

Cuts at:      0    2074    2733 
   Size:       2074     659
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1157    2733 
   Size:       1157    1576
 
BstYI r'GATC_y

Cuts at:      0     246     677     841    2137    2271    2733 
   Size:        246     431     164    1296     134     462

  Fragments arranged by size: 

                1296     462     431     246     164     134
 
Bsu36I CC'TnA_GG

Cuts at:      0     515     638    2733 
   Size:        515     123    2095

  Fragments arranged by size: 

                2095     515     123
 
BtgI C'CryG_G

Cuts at:      0      47     727    2598    2733 
   Size:         47     680    1871     135

  Fragments arranged by size: 

                1871     680     135      47
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2698    2733 
   Size:       2698      35
 
BtsI GCAGTG_nn'

Cuts at:      0     867    1586    2099    2733 
   Size:        867     719     513     634

  Fragments arranged by size: 

                 867     719     634     513
 
EaeI y'GGCC_r

Cuts at:      0     297     484    2733 
   Size:        297     187    2249

  Fragments arranged by size: 

                2249     297     187
 
EarI CTCTTCn'nnn_

Cuts at:      0     120     298     593     704    2733 
   Size:        120     178     295     111    2029

  Fragments arranged by size: 

                2029     295     178     120     111
 
EcoICRI GAG'CTC

Cuts at:      0     603     797    2184    2733 
   Size:        603     194    1387     549

  Fragments arranged by size: 

                1387     603     549     194
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     194     213     239     481     568     617     733 
   Size:        101      93      19      26     242      87      49     116

Cuts at:    733     782     833    1118    1239    1300    2318    2521    2733 
   Size:         49      51     285     121      61    1018     203     212

  Fragments arranged by size: 

                1018     285     242     212     203     121     116     101
                  93      87      61      51      49      49      26      19
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      34    1048    2111    2733 
   Size:         34    1014    1063     622

  Fragments arranged by size: 

                1063    1014     622      34
 
EcoO109I rG'GnC_Cy

Cuts at:      0     284     328     338     509     642     643     783    1303 
   Size:        284      44      10     171     133       1     140     520

Cuts at:   1303    1326    1334    1367    1413    1551    1697    1923    2331 
   Size:         23       8      33      46     138     146     226     408

Cuts at:   2331    2733 
   Size:        402

  Fragments arranged by size: 

                 520     408     402     284     226     171     146     140
                 138     133      46      44      33      23      10       8
                   1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     589    1029    1983    2112    2733 
   Size:        589     440     954     129     621

  Fragments arranged by size: 

                 954     621     589     440     129
 
FseI GG_CCGG'CC

Cuts at:      0      44    2733 
   Size:         44    2689
 
FspI TGC'GCA

Cuts at:      0    2449    2733 
   Size:       2449     284
 
HaeII r_GCGC'y

Cuts at:      0    1158    1965    2733 
   Size:       1158     807     768

  Fragments arranged by size: 

                1158     807     768
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     784     816    1045    1077    2268    2300    2733 
   Size:        784      32     229      32    1191      32     433

  Fragments arranged by size: 

                1191     784     433     229      32      32      32
 
HincII GTy'rAC

Cuts at:      0    2373    2733 
   Size:       2373     360
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     419    2165    2691    2728    2733 
   Size:        419    1746     526      37       5

  Fragments arranged by size: 

                1746     526     419      37       5
 
MslI CAynn'nnrTG

Cuts at:      0      61     412    2654    2733 
   Size:         61     351    2242      79

  Fragments arranged by size: 

                2242     351      79      61
 
MspA1I CmG'CkG

Cuts at:      0    1776    2499    2733 
   Size:       1776     723     234

  Fragments arranged by size: 

                1776     723     234
 
NaeI GCC'GGC

Cuts at:      0      42    2733 
   Size:         42    2691
 
NgoMIV G'CCGG_C

Cuts at:      0      40    2733 
   Size:         40    2693
 
NspI r_CATG'y

Cuts at:      0    2454    2733 
   Size:       2454     279
 
PasI CC'CwG_GG

Cuts at:      0      30    2733 
   Size:         30    2703
 
PfoI T'CCnGG_A

Cuts at:      0      75    2733 
   Size:         75    2658
 
PpuMI rG'GwC_Cy

Cuts at:      0    1303    1334    1413    1923    2331    2733 
   Size:       1303      31      79     510     408     402

  Fragments arranged by size: 

                1303     510     408     402      79      31
 
PsiI TTA'TAA

Cuts at:      0    1727    2733 
   Size:       1727    1006
 
PspOMI G'GGCC_C

Cuts at:      0     642     783    1367    2733 
   Size:        642     141     584    1366

  Fragments arranged by size: 

                1366     642     584     141
 
PstI C_TGCA'G

Cuts at:      0    1625    1775    2166    2498    2733 
   Size:       1625     150     391     332     235

  Fragments arranged by size: 

                1625     391     332     235     150
 
PvuII CAG'CTG

Cuts at:      0    2499    2733 
   Size:       2499     234
 
SacI G_AGCT'C

Cuts at:      0     605     799    2186    2733 
   Size:        605     194    1387     547

  Fragments arranged by size: 

                1387     605     547     194
 
SanDI GG'GwC_CC

Cuts at:      0    2331    2733 
   Size:       2331     402
 
SapI GCTCTTCn'nnn_

Cuts at:      0     593    2733 
   Size:        593    2140
 
ScaI AGT'ACT

Cuts at:      0    1523    2733 
   Size:       1523    1210
 
SfcI C'TryA_G

Cuts at:      0     815    1621    1643    1771    2162    2240    2494    2733 
   Size:        815     806      22     128     391      78     254     239

  Fragments arranged by size: 

                 815     806     391     254     239     128      78      22
            
 
SmlI C'TyrA_G

Cuts at:      0    1027    1417    1968    2733 
   Size:       1027     390     551     765

  Fragments arranged by size: 

                1027     765     551     390
 
SphI G_CATG'C

Cuts at:      0    2454    2733 
   Size:       2454     279
 
StuI AGG'CCT

Cuts at:      0    2092    2733 
   Size:       2092     641
 
StyI C'CwwG_G

Cuts at:      0     280     440     647     941    1329    2079    2345    2733 
   Size:        280     160     207     294     388     750     266     388

  Fragments arranged by size: 

                 750     388     388     294     280     266     207     160
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1295    2733 
   Size:       1295    1438
 
TatI w'GTAC_w

Cuts at:      0    1077    1521    1972    2733 
   Size:       1077     444     451     761

  Fragments arranged by size: 

                1077     761     451     444
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      71     103     294     326     698     730    1131    1163 
   Size:         71      32     191      32     372      32     401      32

Cuts at:   1163    1377    1409    2733 
   Size:        214      32    1324

  Fragments arranged by size: 

                1324     401     372     214     191      71      32      32
                  32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1378    2596    2733 
   Size:       1378    1218     137

  Fragments arranged by size: 

                1378    1218     137
 
ZraI GAC'GTC

Cuts at:      0    1933    2733 
   Size:       1933     800
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI     AloI    AlwNI     ApaI
     ApoI    AvrII     BaeI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     EaeI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FalI     FseI     FspI    HaeII    Hin4I   HincII
     MmeI     MslI   MspA1I     NaeI   NgoMIV     NspI     PasI     PfoI
    PpuMI     PsiI   PspOMI     PstI    PvuII     SacI    SanDI     SapI
     ScaI     SfcI     SmlI     SphI     StuI     StyI    TaqII     TatI
     TstI     XcmI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AleI     AlfI
    ApaLI     AscI     AseI    AsiSI     AvaI     BbeI    BbvCI    BfrBI
    BmgBI     BmtI    BsaAI    BsaBI    BsiEI    BsiWI    BsmBI    BspHI
   BssHII   BstAPI  BstZ17I     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI    EcoRI    EcoRV    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI     XmaI
     XmnI
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