Restriction map for pF1KA0003
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
Asp700I     Asp718I     AspEI       AspHI       AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaHI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspMI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cac8I       CelII       Cfr10I      ClaI        CpoI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HgaI        Hin1I       HindIII     KasI        
KpnI        KspI        MfeI        MluI        MluNI       
MroI        MscI        MunI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmeI        PpuMI       PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        SwaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AciI         1390  GCGG                 CCGC(-3/-1)
AcsI          495  GAATTC               [GA]^AATT[CT]
AlwI          558  GATCC                GGATC(4/5)
ApaI         1441  GGGCCC               GGGCC^C
ApoI          495  GAATTC               [GA]^AATT[CT]
AseI          910  ATTAAT               AT^TAAT
AsnI          910  ATTAAT               AT^TAAT
AspI          766  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanII        1441  GGGCCC               G[GA]GC[CT]^C
BbrPI         217  CACGTG               CAC^GTG
BsaAI         217  CACGTG               [CT]AC^GT[GA]
BsaBI         862  GATGTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          445  GAGACC               GGTCTC(1/5)
BseRI         400  CTCCTC               GAGGAG(10/8)
BsmI          351  GAATGC               GAATGC(1/-1)
BspLU11I      261  ACATGT               A^CATGT
BsrDI         117  GCAATG               GCAATG(2/0)
BstAPI       1107  GCAGTACATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         557  AGATCC               [GA]^GATC[CT]
CfoI           66  GCGC                 GCG^C
DraI         1212  TTTAAA               TTT^AAA
DraII        1440  AGGGCCC              [GA]G^G[ACGT]CC[CT]
DsaI         1401  CCATGG               C^C[GA][CT]GG
EcoO109I     1440  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         495  GAATTC               G^AATTC
EcoT14I      1401  CCATGG               C^C[AT][AT]GG
FauI         1390  GCGGG                CCCGC(4/6)
HaeII          65  AGCGCC               [GA]GCGC^[CT]
HhaI           66  GCGC                 GCG^C
HinP1I         66  GCGC                 G^CGC
HincII       1326  GTTAAC               GT[CT]^[GA]AC
HindII       1326  GTTAAC               GT[CT]^[GA]AC
HpaI         1326  GTTAAC               GTT^AAC
HphI         1057  GGTGA                GGTGA(8/7)
MamI          862  GATGTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          557  AGATCC               [GA]^GATC[CT]
MslI         1464  CACAACTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        484  CAGCTG               C[AC]G^C[GT]G
NcoI         1401  CCATGG               C^CATGG
PflFI         766  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         690  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI           42  GACTC                GAGTC(4/5)
PmaCI         217  CACGTG               CAC^GTG
PmlI          217  CACGTG               CAC^GTG
PshBI         910  ATTAAT               AT^TAAT
PvuII         484  CAGCTG               CAG^CTG
SspI         1061  AATATT               AAT^ATT
StyI         1401  CCATGG               C^C[AT][AT]GG
Tsp45I       1220  GTCAC                ^GT[GC]AC
Tth111I       766  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        690  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1002  TCTAGA               T^CTAGA
XhoII         557  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AflIII        216  ACACGT               A^C[GA][CT]GT
              261  ACATGT              
BfaI          517  CTAG                 C^TAG
             1003  CTAG                
BmyI         1316  GTGCCC               G[AGT]GC[ACT]^C
             1441  GGGCCC              
BsaJI         449  CCCAGG               C^C[ACGT][ACGT]GG
             1401  CCATGG              
BsgI           54  GTGCAG               GTGCAG(16/14)
              857  GTGCAG              
BsiYI         690  CCAGGAGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1097  CCAGTCAGAGG         
BslI          690  CCAGGAGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1097  CCAGTCAGAGG         
BsmAI         445  GAGAC                GTCTC(1/5)
              851  GAGAC               
BsmFI        1228  GGGAC                GGGAC(10/14)
             1392  GGGAC               
Bsp1286I     1316  GTGCCC               G[AGT]GC[ACT]^C
             1441  GGGCCC              
BssKI         450  CCAGG                ^CC[ACGT]GG
              690  CCAGG               
BstF5I        951  GGATG                GGATG(2/0)
             1336  GGATG               
BstNI         450  CCAGG                CC^[AT]GG
              690  CCAGG               
DpnII         558  GATC                 ^GATC
             1254  GATC                
EcoRII        450  CCAGG                ^CC[AT]GG
              690  CCAGG               
FokI          951  GGATG                GGATG(9/13)
             1336  GGATG               
MaeI          517  CTAG                 C^TAG
             1003  CTAG                
MaeII         218  ACGT                 A^CGT
             1458  ACGT                
MboI          558  GATC                 ^GATC
             1254  GATC                
MvaI          450  CCAGG                CC^[AT]GG
              690  CCAGG               
NdeII         558  GATC                 ^GATC
             1254  GATC                
NspI          261  ACATGT               [GA]CATG^[CT]
             1112  ACATGC              
PstI          199  CTGCAG               CTGCA^G
              413  CTGCAG              
Sau3AI        558  GATC                 ^GATC
             1254  GATC                
ScrFI         450  CCAGG                CC^[ACGT]GG
              690  CCAGG               
SmlI          298  CTTGAG               C^T[CT][GA]AG
              475  CTTGAG              
TaqI          471  TCGA                 T^CGA
             1479  TCGA                
TthHB8I       471  TCGA                 T^CGA
             1479  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AfaI        AluI        AlwNI       BbvI        BpmI        
BsaWI       BsrI        Cfr13I      DdeI        EarI        
Fnu4HI      HaeIII      HapII       HinfI       HpaII       
ItaI        Ksp632I     MaeIII      MboII       MnlI        
MseI        MspI        MwoI        NlaIII      NlaIV       
RsaI        Sau96I      SfaNI       SfcI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       
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