Restriction map for
pF1KB7869
(Circular) (Six-base) MAPSORT of: pF1KB7869.seq Check: 3634 from: 1 to: 4796
pF1KB7869 4796 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 1834 1834
Size: 4796
Acc65I G'GTAC_C
Cuts at: 1812 1812
Size: 4796
AclI AA'CG_TT
Cuts at: 4636 4636
Size: 4796
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 905 1275 1359 2621 3053 3800 4068 905
Size: 370 84 1262 432 747 268 1633
Fragments arranged by size:
1633 1262 747 432 370 268 84
AfeI AGC'GCT
Cuts at: 542 1372 1549 542
Size: 830 177 3789
Fragments arranged by size:
3789 830 177
AflIII A'CryG_T
Cuts at: 2148 3252 4229 2148
Size: 1104 977 2715
Fragments arranged by size:
2715 1104 977
AgeI A'CCGG_T
Cuts at: 3184 3184
Size: 4796
AleI CACnn'nnGTG
Cuts at: 589 1250 589
Size: 661 4135
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 989 989
Size: 4796
AlwNI CAG_nnn'CTG
Cuts at: 417 2101 3668 417
Size: 1684 1567 1545
Fragments arranged by size:
1684 1567 1545
ApaLI G'TGCA_C
Cuts at: 3566 3566
Size: 4796
ApoI r'AATT_y
Cuts at: 111 737 876 1383 1658 1742 1800 4149 4405
Size: 626 139 507 275 84 58 2349 256
Cuts at: 4405 111
Size: 502
Fragments arranged by size:
2349 626 507 502 275 256 139 84
58
AseI AT'TA_AT
Cuts at: 20 20
Size: 4796
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
AvaI C'yCGr_G
Cuts at: 391 457 1816 391
Size: 66 1359 3371
Fragments arranged by size:
3371 1359 66
BamHI G'GATC_C
Cuts at: 1821 1821
Size: 4796
BanI G'GyrC_C
Cuts at: 192 1812 2226 2474 2509 192
Size: 1620 414 248 35 2479
Fragments arranged by size:
2479 1620 414 248 35
BanII G_rGCy'C
Cuts at: 260 531 1810 2840 260
Size: 271 1279 1030 2216
Fragments arranged by size:
2216 1279 1030 271
BbeI G_GCGC'C
Cuts at: 196 2230 2478 196
Size: 2034 248 2514
Fragments arranged by size:
2514 2034 248
BbsI GAAGACnn'nnnn_
Cuts at: 242 950 242
Size: 708 4088
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 470 504 470
Size: 34 4762
BciVI GTATCCnnnnn_n'
Cuts at: 2694 3455 4796 2694
Size: 761 1341 2694
Fragments arranged by size:
2694 1341 761
BclI T'GATC_A
Cuts at: 2316 2316
Size: 4796
BglI GCCn_nnn'nGGC
Cuts at: 762 762
Size: 4796
BglII A'GATC_T
Cuts at: 2311 4348 2311
Size: 2037 2759
BlpI GC'TnA_GC
Cuts at: 352 1908 352
Size: 1556 3240
Bme1580I G_kGCm'C
Cuts at: 2421 2514 3570 2421
Size: 93 1056 3647
Fragments arranged by size:
3647 1056 93
BmgBI CAC'GTC
Cuts at: 1082 1082
Size: 4796
BmrI ACTGGGnnnn_n'
Cuts at: 1592 1676 2008 2089 2424 4568 1592
Size: 84 332 81 335 2144 1820
Fragments arranged by size:
2144 1820 335 332 84 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 255 417 3163 255
Size: 162 2746 1888
Fragments arranged by size:
2746 1888 162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 454 439
Size: 15 4781
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1972 2851 3343 3641 1972
Size: 879 492 298 3127
Fragments arranged by size:
3127 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 381 845 1141 4500 381
Size: 464 296 3359 677
Fragments arranged by size:
3359 677 464 296
BsaAI yAC'GTr
Cuts at: 1089 2149 1089
Size: 1060 3736
BsaBI GATnn'nnATC
Cuts at: 6 2310 4347 6
Size: 2304 2037 455
Fragments arranged by size:
2304 2037 455
BsaHI Gr'CG_yC
Cuts at: 146 193 2227 2475 3177 4673 146
Size: 47 2034 248 702 1496 269
Fragments arranged by size:
2034 1496 702 269 248 47
BsaWI w'CCGG_w
Cuts at: 339 847 1985 2506 3184 3458 3605 4016 339
Size: 508 1138 521 678 274 147 411 1119
Fragments arranged by size:
1138 1119 678 521 508 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 475 520 622 475
Size: 45 102 4649
Fragments arranged by size:
4649 102 45
BseYI C'CCAG_C
Cuts at: 627 1768 2221 2979 3556 4052 627
Size: 1141 453 758 577 496 1371
Fragments arranged by size:
1371 1141 758 577 496 453
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 286
Size: 4796
BsiEI CG_ry'CG
Cuts at: 104 2338 2384 3592 104
Size: 2234 46 1208 1308
Fragments arranged by size:
2234 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 488 679 1810 2588 2778 3570 488
Size: 191 1131 778 190 792 1714
Fragments arranged by size:
1714 1131 792 778 191 190
BsmI GAATG_Cn'
Cuts at: 1450 1531 1450
Size: 81 4715
Bsp1286I G_dGCh'C
Cuts at: 260 488 531 679 1810 2421 2514 2588 2778
Size: 228 43 148 1131 611 93 74 190
Cuts at: 2778 2840 3570 260
Size: 62 730 1486
Fragments arranged by size:
1486 1131 730 611 228 190 148 93
74 62 43
BspEI T'CCGG_A
Cuts at: 847 4016 847
Size: 3169 1627
BspHI T'CATG_A
Cuts at: 3972 3972
Size: 4796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1846 2060 2362 2743 1846
Size: 214 302 381 3899
Fragments arranged by size:
3899 381 302 214
BsrBI CCG'CTC
Cuts at: 50 500 3088 3142 4626 4791 50
Size: 450 2588 54 1484 165 55
Fragments arranged by size:
2588 1484 450 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2708 2708
Size: 4796
BsrFI r'CCGG_y
Cuts at: 356 2794 2975 3184 356
Size: 2438 181 209 1968
Fragments arranged by size:
2438 1968 209 181
BssSI C'ACGA_G
Cuts at: 3067 3425 3067
Size: 358 4438
BstAPI GCAn_nnn'nTGC
Cuts at: 2173 2173
Size: 4796
BstBI TT'CG_AA
Cuts at: 3157 3157
Size: 4796
BstXI CCAn_nnnn'nTGG
Cuts at: 1279 1279
Size: 4796
BstYI r'GATC_y
Cuts at: 1 413 1821 2288 2311 2646 2892 3904 3990
Size: 412 1408 467 23 335 246 1012 86
Cuts at: 3990 4002 4348 1
Size: 12 346 449
Fragments arranged by size:
1408 1012 467 449 412 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 269 518 269
Size: 249 4547
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2746 2927 3090 2746
Size: 181 163 4452
Fragments arranged by size:
4452 181 163
BtsI GCAGTG_nn'
Cuts at: 147 486 2039 147
Size: 339 1553 2904
Fragments arranged by size:
2904 1553 339
DraI TTT'AAA
Cuts at: 1434 1563 1795 1434
Size: 129 232 4435
Fragments arranged by size:
4435 232 129
DraIII CAC_nnn'GTG
Cuts at: 1077 1077
Size: 4796
DrdI GACnn_nn'nnGTC
Cuts at: 2502 3360 2502
Size: 858 3938
EaeI y'GGCC_r
Cuts at: 515 2381 2555 2946 2973 4318 515
Size: 1866 174 391 27 1345 993
Fragments arranged by size:
1866 1345 993 391 174 27
EagI C'GGCC_G
Cuts at: 2381 2381
Size: 4796
EarI CTCTTCn'nnn_
Cuts at: 338 1195 1528 2819 3029 338
Size: 857 333 1291 210 2105
Fragments arranged by size:
2105 1291 857 333 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 674 3004 3314 3460 4606 674
Size: 2330 310 146 1146 864
Fragments arranged by size:
2330 1146 864 310 146
EcoICRI GAG'CTC
Cuts at: 1808 1808
Size: 4796
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 454 905 1275 1359 2621 3053 3800 4068
Size: 15 451 370 84 1262 432 747 268
Cuts at: 4068 439
Size: 1167
Fragments arranged by size:
1262 1167 747 451 432 370 268 84
15
EcoNI CCTnn'n_nnAGG
Cuts at: 457 457
Size: 4796
EcoO109I rG'GnC_Cy
Cuts at: 157 1935 157
Size: 1778 3018
EcoRI G'AATT_C
Cuts at: 111 1383 1658 1742 1800 111
Size: 1272 275 84 58 3107
Fragments arranged by size:
3107 1272 275 84 58
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 4796
FspI TGC'GCA
Cuts at: 659 2577 659
Size: 1918 2878
HaeII r_GCGC'y
Cuts at: 196 352 544 621 795 1374 1551 2230 2478
Size: 156 192 77 174 579 177 679 248
Cuts at: 2478 3500 4479 196
Size: 1022 979 513
Fragments arranged by size:
1022 979 679 579 513 248 192 177
174 156 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 538 570 1592 1624 2638 2668 2670 2700 538
Size: 32 1022 32 1014 30 2 30 2634
Fragments arranged by size:
2634 1022 1014 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1835 1835
Size: 4796
HindIII A'AGCT_T
Cuts at: 1287 1287
Size: 4796
KasI G'GCGC_C
Cuts at: 192 2226 2474 192
Size: 2034 248 2514
Fragments arranged by size:
2514 2034 248
KpnI G_GTAC'C
Cuts at: 1816 1816
Size: 4796
MluI A'CGCG_T
Cuts at: 4229 4229
Size: 4796
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1010 1493 1755 3467 3651 1010
Size: 483 262 1712 184 2155
Fragments arranged by size:
2155 1712 483 262 184
MscI TGG'CCA
Cuts at: 2557 2557
Size: 4796
MslI CAynn'nnrTG
Cuts at: 589 1250 2785 2912 589
Size: 661 1535 127 2473
Fragments arranged by size:
2473 1535 661 127
MspA1I CmG'CkG
Cuts at: 271 315 1907 2221 2581 3594 3839 271
Size: 44 1592 314 360 1013 245 1228
Fragments arranged by size:
1592 1228 1013 360 314 245 44
NaeI GCC'GGC
Cuts at: 358 2977 358
Size: 2619 2177
NarI GG'CG_CC
Cuts at: 193 2227 2475 193
Size: 2034 248 2514
Fragments arranged by size:
2514 2034 248
NgoMIV G'CCGG_C
Cuts at: 356 2975 356
Size: 2619 2177
NspI r_CATG'y
Cuts at: 1849 2349 3256 1849
Size: 500 907 3389
Fragments arranged by size:
3389 907 500
PasI CC'CwG_GG
Cuts at: 161 561 161
Size: 400 4396
PciI A'CATG_T
Cuts at: 3252 3252
Size: 4796
PflMI CCAn_nnn'nTGG
Cuts at: 330 567 330
Size: 237 4559
PfoI T'CCnGG_A
Cuts at: 416 670 416
Size: 254 4542
PmeI GTTT'AAAC
Cuts at: 1795 1795
Size: 4796
PmlI CAC'GTG
Cuts at: 1089 1089
Size: 4796
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 3935
Size: 32 4764
PpuMI rG'GwC_Cy
Cuts at: 157 157
Size: 4796
PsiI TTA'TAA
Cuts at: 1181 1181
Size: 4796
PstI C_TGCA'G
Cuts at: 536 649 1843 2528 536
Size: 113 1194 685 2804
Fragments arranged by size:
2804 1194 685 113
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4796
PvuII CAG'CTG
Cuts at: 315 2221 2581 315
Size: 1906 360 2530
Fragments arranged by size:
2530 1906 360
RsrII CG'GwC_CG
Cuts at: 2991 2991
Size: 4796
SacI G_AGCT'C
Cuts at: 1810 1810
Size: 4796
SacII CC_GC'GG
Cuts at: 272 272
Size: 4796
SalI G'TCGA_C
Cuts at: 1833 1833
Size: 4796
SanDI GG'GwC_CC
Cuts at: 157 157
Size: 4796
SapI GCTCTTCn'nnn_
Cuts at: 1528 2819 3029 1528
Size: 1291 210 3295
Fragments arranged by size:
3295 1291 210
SbfI CC_TGCA'GG
Cuts at: 1843 1843
Size: 4796
ScaI AGT'ACT
Cuts at: 4313 4313
Size: 4796
SfcI C'TryA_G
Cuts at: 33 532 645 1839 2524 3517 3708 33
Size: 499 113 1194 685 993 191 1121
Fragments arranged by size:
1194 1121 993 685 499 191 113
SfoI GGC'GCC
Cuts at: 194 2228 2476 194
Size: 2034 248 2514
Fragments arranged by size:
2514 2034 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
SmaI CCC'GGG
Cuts at: 1818 1818
Size: 4796
SmlI C'TyrA_G
Cuts at: 1951 2866 3358 3620 1951
Size: 915 492 262 3127
Fragments arranged by size:
3127 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4796
SphI G_CATG'C
Cuts at: 1849 2349 1849
Size: 500 4296
StyI C'CwwG_G
Cuts at: 182 404 1930 2284 182
Size: 222 1526 354 2694
Fragments arranged by size:
2694 1526 354 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 153 465 2379 2512 2971 3180 153
Size: 312 1914 133 459 209 1769
Fragments arranged by size:
1914 1769 459 312 209 133
TatI w'GTAC_w
Cuts at: 1717 4311 1717
Size: 2594 2202
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 149 181 1712 1744 4313 4345 149
Size: 32 1531 32 2569 32 600
Fragments arranged by size:
2569 1531 600 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2593 2593
Size: 4796
XbaI T'CTAG_A
Cuts at: 1827 1827
Size: 4796
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 331 2291 331
Size: 1960 2836
XmaI C'CCGG_G
Cuts at: 1816 1816
Size: 4796
XmnI GAAnn'nnTTC
Cuts at: 1204 1288 1537 1621 1204
Size: 84 249 84 4379
Fragments arranged by size:
4379 249 84 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BmtI Bpu10I BsaXI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PshAI PspOMI PspXI PsrI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI