Restriction map for pF1KB9574
 (Circular) (Six-base) MAPSORT of: pF1KB9574.seq  Check: 774  from: 1  to: 4253



pF1KB9574  4253 bp

 With 182 enzymes: SgfI * 

                        February 20, 2009 16:43  ..

AccI GT'mk_AC

Cuts at:   1291    1291 
   Size:       4253
 
Acc65I G'GTAC_C

Cuts at:   1269    1269 
   Size:       4253
 
AclI AA'CG_TT

Cuts at:   4093    4093 
   Size:       4253
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    688     919    2078    2510    3257    3525     688 
   Size:        231    1159     432     747     268    1416

  Fragments arranged by size: 

                1416    1159     747     432     268     231
 
AflIII A'CryG_T

Cuts at:   1118    1605    2709    3686    1118 
   Size:        487    1104     977    1685

  Fragments arranged by size: 

                1685    1104     977     487
 
AgeI A'CCGG_T

Cuts at:   2641    2641 
   Size:       4253
 
AleI CACnn'nnGTG

Cuts at:   1084    1084 
   Size:       4253
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    582     582 
   Size:       4253
 
AlwNI CAG_nnn'CTG

Cuts at:    333    1558    3125     333 
   Size:       1225    1567    1461

  Fragments arranged by size: 

                1567    1461    1225
 
ApaI G_GGCC'C

Cuts at:   1052    1052 
   Size:       4253
 
ApaLI G'TGCA_C

Cuts at:   1077    3023    1077 
   Size:       1946    2307
 
ApoI r'AATT_y

Cuts at:   1257    3606    3862    1257 
   Size:       2349     256    1648

  Fragments arranged by size: 

                2349    1648     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4253
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4253
 
AvaI C'yCGr_G

Cuts at:    117    1144    1273     117 
   Size:       1027     129    3097

  Fragments arranged by size: 

                3097    1027     129
 
BamHI G'GATC_C

Cuts at:   1278    1278 
   Size:       4253
 
BanI G'GyrC_C

Cuts at:   1269    1683    1931    1966    1269 
   Size:        414     248      35    3556

  Fragments arranged by size: 

                3556     414     248      35
 
BanII G_rGCy'C

Cuts at:    232     384     854     911    1052    1073    1267    2297     232 
   Size:        152     470      57     141      21     194    1030    2188

  Fragments arranged by size: 

                2188    1030     470     194     152     141      57      21
            
 
BbeI G_GCGC'C

Cuts at:   1687    1935    1687 
   Size:        248    4005
 
BbsI GAAGACnn'nnnn_

Cuts at:    451     907    1115    1125     451 
   Size:        456     208      10    3579

  Fragments arranged by size: 

                3579     456     208      10
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    698     732     698 
   Size:         34    4219
 
BciVI GTATCCnnnnn_n'

Cuts at:   2151    2912    4253    2151 
   Size:        761    1341    2151

  Fragments arranged by size: 

                2151    1341     761
 
BclI T'GATC_A

Cuts at:   1773    1773 
   Size:       4253
 
BglII A'GATC_T

Cuts at:   1768    3805    1768 
   Size:       2037    2216
 
BlpI GC'TnA_GC

Cuts at:   1222    1365    1222 
   Size:        143    4110
 
Bme1580I G_kGCm'C

Cuts at:   1052    1081    1878    1971    3027    1052 
   Size:         29     797      93    1056    2278

  Fragments arranged by size: 

                2278    1056     797      93      29
 
BmgBI CAC'GTC

Cuts at:   1121    1121 
   Size:       4253
 
BmrI ACTGGGnnnn_n'

Cuts at:    788     941    1023    1465    1546    1881    4025     788 
   Size:        153      82     442      81     335    2144    1016

  Fragments arranged by size: 

                2144    1016     442     335     153      82      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1170    1227    2620    1170 
   Size:         57    1393    2803

  Fragments arranged by size: 

                2803    1393      57
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    204     258     411     491    1087     204 
   Size:         54     153      80     596    3370

  Fragments arranged by size: 

                3370     596     153      80      54
 
Bpu10I CC'TnA_GC

Cuts at:    848     848 
   Size:       4253
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1429    2308    2800    3098    1429 
   Size:        879     492     298    2584

  Fragments arranged by size: 

                2584     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3957    3957 
   Size:       4253
 
BsaAI yAC'GTr

Cuts at:   1606    1606 
   Size:       4253
 
BsaBI GATnn'nnATC

Cuts at:      6    1767    3804       6 
   Size:       1761    2037     455

  Fragments arranged by size: 

                2037    1761     455
 
BsaHI Gr'CG_yC

Cuts at:    142    1684    1932    2634    4130     142 
   Size:       1542     248     702    1496     265

  Fragments arranged by size: 

                1542    1496     702     265     248
 
BsaWI w'CCGG_w

Cuts at:    318     575    1083    1442    1963    2641    2915    3062    3473 
   Size:        257     508     359     521     678     274     147     411

Cuts at:   3473     318 
   Size:       1098

  Fragments arranged by size: 

                1098     678     521     508     411     359     274     257
                 147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    202     232    1018    1048    1060    1083    1090    1113     202 
   Size:         30     786      30      12      23       7      23    3342

  Fragments arranged by size: 

                3342     786      30      30      23      23      12       7
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    345     594     826     829     345 
   Size:        249     232       3    3769

  Fragments arranged by size: 

                3769     249     232       3
 
BseYI C'CCAG_C

Cuts at:    415     930    1102    1162    1678    2436    3013    3509     415 
   Size:        515     172      60     516     758     577     496    1159

  Fragments arranged by size: 

                1159     758     577     516     515     496     172      60
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    240     358    1009    1092    1190     240 
   Size:        118     651      83      98    3303

  Fragments arranged by size: 

                3303     651     118      98      83
 
BsiEI CG_ry'CG

Cuts at:    104     176    1795    1841    3049     104 
   Size:         72    1619      46    1208    1308

  Fragments arranged by size: 

                1619    1308    1208      72      46
 
BsiHKAI G_wGCw'C

Cuts at:   1081    1267    2045    2235    3027    1081 
   Size:        186     778     190     792    2307

  Fragments arranged by size: 

                2307     792     778     190     186
 
BsmBI CGTCTCn'nnnn_

Cuts at:    276     357     276 
   Size:         81    4172
 
Bsp1286I G_dGCh'C

Cuts at:    232     384     854     911    1052    1073    1081    1267    1878 
   Size:        152     470      57     141      21       8     186     611

Cuts at:   1878    1971    2045    2235    2297    3027     232 
   Size:         93      74     190      62     730    1458

  Fragments arranged by size: 

                1458     730     611     470     190     186     152     141
                  93      74      62      57      21       8
 
BspEI T'CCGG_A

Cuts at:    318    3473     318 
   Size:       3155    1098
 
BspHI T'CATG_A

Cuts at:   3429    3429 
   Size:       4253
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    332     671     720    1303    1517    1819    2200     332 
   Size:        339      49     583     214     302     381    2385

  Fragments arranged by size: 

                2385     583     381     339     302     214      49
 
BsrBI CCG'CTC

Cuts at:     50     968    2545    2599    4083    4248      50 
   Size:        918    1577      54    1484     165      55

  Fragments arranged by size: 

                1577    1484     918     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1206    2165    1206 
   Size:        959    3294
 
BsrFI r'CCGG_y

Cuts at:   2251    2432    2641    2251 
   Size:        181     209    3863

  Fragments arranged by size: 

                3863     209     181
 
BssSI C'ACGA_G

Cuts at:   2524    2882    2524 
   Size:        358    3895
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1630    1630 
   Size:       4253
 
BstBI TT'CG_AA

Cuts at:   2614    2614 
   Size:       4253
 
BstEII G'GTnAC_C

Cuts at:   1089    1089 
   Size:       4253
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1065    1239    1065 
   Size:        174    4079
 
BstYI r'GATC_y

Cuts at:      1     722    1278    1745    1768    2103    2349    3361    3447 
   Size:        721     556     467      23     335     246    1012      86

Cuts at:   3447    3459    3805       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     721     556     467     449     346     335     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    293    1052     293 
   Size:        759    3494
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    353    2203    2384    2547     353 
   Size:       1850     181     163    2059

  Fragments arranged by size: 

                2059    1850     181     163
 
BtsI GCAGTG_nn'

Cuts at:    299     334    1109    1496     299 
   Size:         35     775     387    3056

  Fragments arranged by size: 

                3056     775     387      35
 
DraI TTT'AAA

Cuts at:   1252    1252 
   Size:       4253
 
DrdI GACnn_nn'nnGTC

Cuts at:    268    1959    2817     268 
   Size:       1691     858    1704

  Fragments arranged by size: 

                1704    1691     858
 
EaeI y'GGCC_r

Cuts at:    173     266    1055    1838    2012    2403    2430    3775     173 
   Size:         93     789     783     174     391      27    1345     651

  Fragments arranged by size: 

                1345     789     783     651     391     174      93      27
            
 
EagI C'GGCC_G

Cuts at:    173    1838     173 
   Size:       1665    2588
 
EarI CTCTTCn'nnn_

Cuts at:    512     743    2276    2486     512 
   Size:        231    1533     210    2279

  Fragments arranged by size: 

                2279    1533     231     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2461    2771    2917    4063    2461 
   Size:        310     146    1146    2651

  Fragments arranged by size: 

                2651    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1265    1265 
   Size:       4253
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    204     258     411     491     688     919    1087    2078    2510 
   Size:         54     153      80     197     231     168     991     432

Cuts at:   2510    3257    3525     204 
   Size:        747     268     932

  Fragments arranged by size: 

                 991     932     747     432     268     231     197     168
                 153      80      54
 
EcoNI CCTnn'n_nnAGG

Cuts at:    667     790     667 
   Size:        123    4130
 
EcoO109I rG'GnC_Cy

Cuts at:    783     995    1048    1392     783 
   Size:        212      53     344    3644

  Fragments arranged by size: 

                3644     344     212      53
 
EcoRI G'AATT_C

Cuts at:   1257    1257 
   Size:       4253
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2272    2272 
   Size:       4253
 
FspI TGC'GCA

Cuts at:   2034    2034 
   Size:       4253
 
HaeII r_GCGC'y

Cuts at:   1687    1935    2957    3936    1687 
   Size:        248    1022     979    2004

  Fragments arranged by size: 

                2004    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    130     162     172     202     204     234     619     651    2095 
   Size:         32      10      30       2      30     385      32    1444

Cuts at:   2095    2125    2127    2157     130 
   Size:         30       2      30    2226

  Fragments arranged by size: 

                2226    1444     385      32      32      30      30      30
                  30      10       2       2
 
HincII GTy'rAC

Cuts at:    240    1292     240 
   Size:       1052    3201
 
KasI G'GCGC_C

Cuts at:   1683    1931    1683 
   Size:        248    4005
 
KpnI G_GTAC'C

Cuts at:   1273    1273 
   Size:       4253
 
MluI A'CGCG_T

Cuts at:   3686    3686 
   Size:       4253
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2924    3108    2924 
   Size:        184    4069
 
MscI TGG'CCA

Cuts at:   1057    2014    1057 
   Size:        957    3296
 
MslI CAynn'nnrTG

Cuts at:   1084    2242    2369    1084 
   Size:       1158     127    2968

  Fragments arranged by size: 

                2968    1158     127
 
MspA1I CmG'CkG

Cuts at:    155     415    1106    1166    1174    1364    1678    2038    3051 
   Size:        260     691      60       8     190     314     360    1013

Cuts at:   3051    3296     155 
   Size:        245    1112

  Fragments arranged by size: 

                1112    1013     691     360     314     260     245     190
                  60       8
 
NaeI GCC'GGC

Cuts at:   2434    2434 
   Size:       4253
 
NarI GG'CG_CC

Cuts at:   1684    1932    1684 
   Size:        248    4005
 
NcoI C'CATG_G

Cuts at:   1052    1052 
   Size:       4253
 
NgoMIV G'CCGG_C

Cuts at:   2432    2432 
   Size:       4253
 
NspI r_CATG'y

Cuts at:   1306    1806    2713    1306 
   Size:        500     907    2846

  Fragments arranged by size: 

                2846     907     500
 
PasI CC'CwG_GG

Cuts at:   1038    1038 
   Size:       4253
 
PciI A'CATG_T

Cuts at:   2709    2709 
   Size:       4253
 
PmeI GTTT'AAAC

Cuts at:   1252    1252 
   Size:       4253
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3392    3424    3392 
   Size:         32    4221
 
PpuMI rG'GwC_Cy

Cuts at:    995     995 
   Size:       4253
 
PspOMI G'GGCC_C

Cuts at:   1048    1048 
   Size:       4253
 
PstI C_TGCA'G

Cuts at:    160     329     561    1300    1985     160 
   Size:        169     232     739     685    2428

  Fragments arranged by size: 

                2428     739     685     232     169
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4253
 
PvuII CAG'CTG

Cuts at:   1106    1166    1174    1678    2038    1106 
   Size:         60       8     504     360    3321

  Fragments arranged by size: 

                3321     504     360      60       8
 
RsrII CG'GwC_CG

Cuts at:   2448    2448 
   Size:       4253
 
SacI G_AGCT'C

Cuts at:   1267    1267 
   Size:       4253
 
SalI G'TCGA_C

Cuts at:   1290    1290 
   Size:       4253
 
SapI GCTCTTCn'nnn_

Cuts at:   2276    2486    2276 
   Size:        210    4043
 
SbfI CC_TGCA'GG

Cuts at:   1300    1300 
   Size:       4253
 
ScaI AGT'ACT

Cuts at:   3770    3770 
   Size:       4253
 
SfcI C'TryA_G

Cuts at:     33     156     325     557    1296    1981    2974    3165      33 
   Size:        123     169     232     739     685     993     191    1121

  Fragments arranged by size: 

                1121     993     739     685     232     191     169     123
            
 
SfoI GGC'GCC

Cuts at:   1685    1933    1685 
   Size:        248    4005
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4253
 
SmaI CCC'GGG

Cuts at:   1275    1275 
   Size:       4253
 
SmlI C'TyrA_G

Cuts at:   1408    2323    2815    3077    1408 
   Size:        915     492     262    2584

  Fragments arranged by size: 

                2584     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4253
 
SphI G_CATG'C

Cuts at:   1306    1806    1306 
   Size:        500    3753
 
StuI AGG'CCT

Cuts at:    820     820 
   Size:       4253
 
StyI C'CwwG_G

Cuts at:   1052    1387    1741    1052 
   Size:        335     354    3564

  Fragments arranged by size: 

                3564     354     335
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    664     919    1245    1836    1969    2428    2637     664 
   Size:        255     326     591     133     459     209    2280

  Fragments arranged by size: 

                2280     591     459     326     255     209     133
 
TatI w'GTAC_w

Cuts at:   1096    3768    1096 
   Size:       2672    1581
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    313     345    1018    1050    1060    1083    1092    1115    3770 
   Size:         32     673      32      10      23       9      23    2655

Cuts at:   3770    3802     313 
   Size:         32     764

  Fragments arranged by size: 

                2655     764     673      32      32      32      23      23
                  10       9
 
Tth111I GACn'n_nGTC

Cuts at:   2050    2050 
   Size:       4253
 
XbaI T'CTAG_A

Cuts at:   1284    1284 
   Size:       4253
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    861    1748     861 
   Size:        887    3366
 
XmaI C'CCGG_G

Cuts at:   1273    1273 
   Size:       4253
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PciI     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AloI     AscI    AvrII
     BaeI    BbvCI    BfrBI     BglI     BmtI    BsiWI     BsmI    BsrGI
   BssHII  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI    PflMI     PfoI     PmlI    PshAI     PsiI    PspXI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     SwaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com