Restriction map for
pF1KB9518
(Circular) (Six-base) MAPSORT of: pF1KB9518.seq Check: 8129 from: 1 to: 4904
pF1KB9518 4904 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1942 1942
Size: 4904
Acc65I G'GTAC_C
Cuts at: 1920 1920
Size: 4904
AclI AA'CG_TT
Cuts at: 4744 4744
Size: 4904
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 248 358 985 2729 3161 3908 4176 208
Size: 40 110 627 1744 432 747 268 936
Fragments arranged by size:
1744 936 747 627 432 268 110 40
AflIII A'CryG_T
Cuts at: 2256 3360 4337 2256
Size: 1104 977 2823
Fragments arranged by size:
2823 1104 977
AgeI A'CCGG_T
Cuts at: 3292 3292
Size: 4904
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1157 1189 1157
Size: 32 4872
AlwNI CAG_nnn'CTG
Cuts at: 2209 3776 2209
Size: 1567 3337
ApaLI G'TGCA_C
Cuts at: 3674 3674
Size: 4904
ApoI r'AATT_y
Cuts at: 1084 1204 1389 1500 1660 1908 4257 4513 1084
Size: 120 185 111 160 248 2349 256 1475
Fragments arranged by size:
2349 1475 256 248 185 160 120 111
AseI AT'TA_AT
Cuts at: 20 20
Size: 4904
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4904
AvaI C'yCGr_G
Cuts at: 742 1924 742
Size: 1182 3722
BamHI G'GATC_C
Cuts at: 1929 1929
Size: 4904
BanI G'GyrC_C
Cuts at: 1920 2334 2582 2617 1920
Size: 414 248 35 4207
Fragments arranged by size:
4207 414 248 35
BanII G_rGCy'C
Cuts at: 1307 1859 1918 2948 1307
Size: 552 59 1030 3263
Fragments arranged by size:
3263 1030 552 59
BbeI G_GCGC'C
Cuts at: 2338 2586 2338
Size: 248 4656
BbsI GAAGACnn'nnnn_
Cuts at: 196 707 1303 1674 196
Size: 511 596 371 3426
Fragments arranged by size:
3426 596 511 371
BciVI GTATCCnnnnn_n'
Cuts at: 2802 3563 4904 2802
Size: 761 1341 2802
Fragments arranged by size:
2802 1341 761
BclI T'GATC_A
Cuts at: 2424 2424
Size: 4904
BfrBI ATG'CAT
Cuts at: 1113 1113
Size: 4904
BglI GCCn_nnn'nGGC
Cuts at: 135 135
Size: 4904
BglII A'GATC_T
Cuts at: 1101 2419 4456 1101
Size: 1318 2037 1549
Fragments arranged by size:
2037 1549 1318
BlpI GC'TnA_GC
Cuts at: 2016 2016
Size: 4904
Bme1580I G_kGCm'C
Cuts at: 400 2529 2622 3678 400
Size: 2129 93 1056 1626
Fragments arranged by size:
2129 1626 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 799 1310 1487 2116 2197 2532 4676 799
Size: 511 177 629 81 335 2144 1027
Fragments arranged by size:
2144 1027 629 511 335 177 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3271 3271
Size: 4904
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 935 935
Size: 4904
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 818 1799 2080 2959 3451 3749 818
Size: 981 281 879 492 298 1973
Fragments arranged by size:
1973 981 879 492 298 281
BsaI GGTCTCn'nnnn_
Cuts at: 412 4608 412
Size: 4196 708
BsaAI yAC'GTr
Cuts at: 2257 2257
Size: 4904
BsaBI GATnn'nnATC
Cuts at: 6 2418 4455 6
Size: 2412 2037 455
Fragments arranged by size:
2412 2037 455
BsaHI Gr'CG_yC
Cuts at: 2335 2583 3285 4781 2335
Size: 248 702 1496 2458
Fragments arranged by size:
2458 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2093 2614 3292 3566 3713 4124 2093
Size: 521 678 274 147 411 2873
Fragments arranged by size:
2873 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1765 1795 1765
Size: 30 4874
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1553 1553
Size: 4904
BseYI C'CCAG_C
Cuts at: 247 893 1676 2329 3087 3664 4160 247
Size: 646 783 653 758 577 496 991
Fragments arranged by size:
991 783 758 653 646 577 496
BsiEI CG_ry'CG
Cuts at: 104 2446 2492 3700 104
Size: 2342 46 1208 1308
Fragments arranged by size:
2342 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 921 1859 1918 2696 2886 3678 921
Size: 938 59 778 190 792 2147
Fragments arranged by size:
2147 938 792 778 190 59
BsmI GAATG_Cn'
Cuts at: 1660 1660
Size: 4904
Bsp1286I G_dGCh'C
Cuts at: 400 921 1307 1859 1918 2529 2622 2696 2886
Size: 521 386 552 59 611 93 74 190
Cuts at: 2886 2948 3678 400
Size: 62 730 1626
Fragments arranged by size:
1626 730 611 552 521 386 190 93
74 62 59
BspEI T'CCGG_A
Cuts at: 4124 4124
Size: 4904
BspHI T'CATG_A
Cuts at: 1685 4080 1685
Size: 2395 2509
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1954 2168 2470 2851 1954
Size: 214 302 381 4007
Fragments arranged by size:
4007 381 302 214
BsrBI CCG'CTC
Cuts at: 50 441 3196 3250 4734 4899 50
Size: 391 2755 54 1484 165 55
Fragments arranged by size:
2755 1484 391 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2816 2816
Size: 4904
BsrFI r'CCGG_y
Cuts at: 162 2902 3083 3292 162
Size: 2740 181 209 1774
Fragments arranged by size:
2740 1774 209 181
BssSI C'ACGA_G
Cuts at: 3175 3533 3175
Size: 358 4546
BstAPI GCAn_nnn'nTGC
Cuts at: 565 2281 565
Size: 1716 3188
BstBI TT'CG_AA
Cuts at: 3265 3265
Size: 4904
BstYI r'GATC_y
Cuts at: 1 122 1101 1929 2396 2419 2754 3000 4012
Size: 121 979 828 467 23 335 246 1012
Cuts at: 4012 4098 4110 4456 1
Size: 86 12 346 449
Fragments arranged by size:
1012 979 828 467 449 346 335 246
121 86 23 12
BtgI C'CryG_G
Cuts at: 107 130 932 1733 107
Size: 23 802 801 3278
Fragments arranged by size:
3278 802 801 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2854 3035 3198 2854
Size: 181 163 4560
Fragments arranged by size:
4560 181 163
BtsI GCAGTG_nn'
Cuts at: 1258 1727 2147 1258
Size: 469 420 4015
Fragments arranged by size:
4015 469 420
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 653 688 653
Size: 35 4869
DraI TTT'AAA
Cuts at: 1903 1903
Size: 4904
DrdI GACnn_nn'nnGTC
Cuts at: 2610 3468 2610
Size: 858 4046
EaeI y'GGCC_r
Cuts at: 156 2489 2663 3054 3081 4426 156
Size: 2333 174 391 27 1345 634
Fragments arranged by size:
2333 1345 634 391 174 27
EagI C'GGCC_G
Cuts at: 2489 2489
Size: 4904
EarI CTCTTCn'nnn_
Cuts at: 2927 3137 2927
Size: 210 4694
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3112 3422 3568 4714 3112
Size: 310 146 1146 3302
Fragments arranged by size:
3302 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1857 1916 1857
Size: 59 4845
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 248 358 935 985 2729 3161 3908 4176
Size: 40 110 577 50 1744 432 747 268
Cuts at: 4176 208
Size: 936
Fragments arranged by size:
1744 936 747 577 432 268 110 50
40
EcoO109I rG'GnC_Cy
Cuts at: 2043 2043
Size: 4904
EcoRI G'AATT_C
Cuts at: 1500 1908 1500
Size: 408 4496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 773 1567 2923 773
Size: 794 1356 2754
Fragments arranged by size:
2754 1356 794
FspI TGC'GCA
Cuts at: 2685 2685
Size: 4904
HaeII r_GCGC'y
Cuts at: 797 2338 2586 3608 4587 797
Size: 1541 248 1022 979 1114
Fragments arranged by size:
1541 1114 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2746 2776 2778 2808 2746
Size: 30 2 30 4842
Fragments arranged by size:
4842 30 30 2
HincII GTy'rAC
Cuts at: 1943 1943
Size: 4904
KasI G'GCGC_C
Cuts at: 2334 2582 2334
Size: 248 4656
KpnI G_GTAC'C
Cuts at: 1924 1924
Size: 4904
MluI A'CGCG_T
Cuts at: 4337 4337
Size: 4904
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1203 1560 3575 3759 1203
Size: 357 2015 184 2348
Fragments arranged by size:
2348 2015 357 184
MscI TGG'CCA
Cuts at: 2665 2665
Size: 4904
MslI CAynn'nnrTG
Cuts at: 1732 1738 2893 3020 1732
Size: 6 1155 127 3616
Fragments arranged by size:
3616 1155 127 6
MspA1I CmG'CkG
Cuts at: 132 1676 2015 2329 2689 3702 3947 132
Size: 1544 339 314 360 1013 245 1089
Fragments arranged by size:
1544 1089 1013 360 339 314 245
NaeI GCC'GGC
Cuts at: 3085 3085
Size: 4904
NarI GG'CG_CC
Cuts at: 2335 2583 2335
Size: 248 4656
NcoI C'CATG_G
Cuts at: 107 932 1733 107
Size: 825 801 3278
Fragments arranged by size:
3278 825 801
NdeI CA'TA_TG
Cuts at: 517 517
Size: 4904
NgoMIV G'CCGG_C
Cuts at: 3083 3083
Size: 4904
NsiI A_TGCA'T
Cuts at: 1115 1115
Size: 4904
NspI r_CATG'y
Cuts at: 255 1957 2457 3364 255
Size: 1702 500 907 1795
Fragments arranged by size:
1795 1702 907 500
PciI A'CATG_T
Cuts at: 3360 3360
Size: 4904
PflMI CCAn_nnn'nTGG
Cuts at: 165 1416 165
Size: 1251 3653
PmeI GTTT'AAAC
Cuts at: 1903 1903
Size: 4904
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 4043
Size: 32 4872
PsiI TTA'TAA
Cuts at: 1273 1273
Size: 4904
PstI C_TGCA'G
Cuts at: 1253 1809 1951 2636 1253
Size: 556 142 685 3521
Fragments arranged by size:
3521 685 556 142
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4904
PvuII CAG'CTG
Cuts at: 1676 2329 2689 1676
Size: 653 360 3891
Fragments arranged by size:
3891 653 360
RsrII CG'GwC_CG
Cuts at: 3099 3099
Size: 4904
SacI G_AGCT'C
Cuts at: 1859 1918 1859
Size: 59 4845
SacII CC_GC'GG
Cuts at: 133 133
Size: 4904
SalI G'TCGA_C
Cuts at: 1941 1941
Size: 4904
SapI GCTCTTCn'nnn_
Cuts at: 2927 3137 2927
Size: 210 4694
SbfI CC_TGCA'GG
Cuts at: 1951 1951
Size: 4904
ScaI AGT'ACT
Cuts at: 4421 4421
Size: 4904
SexAI A'CCwGG_T
Cuts at: 1455 1455
Size: 4904
SfcI C'TryA_G
Cuts at: 33 1249 1805 1947 2632 3625 3816 33
Size: 1216 556 142 685 993 191 1121
Fragments arranged by size:
1216 1121 993 685 556 191 142
SfoI GGC'GCC
Cuts at: 2336 2584 2336
Size: 248 4656
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4904
SmaI CCC'GGG
Cuts at: 744 1926 744
Size: 1182 3722
SmlI C'TyrA_G
Cuts at: 833 1814 2059 2974 3466 3728 833
Size: 981 245 915 492 262 2009
Fragments arranged by size:
2009 981 915 492 262 245
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4904
SphI G_CATG'C
Cuts at: 255 1957 2457 255
Size: 1702 500 2702
Fragments arranged by size:
2702 1702 500
SspI AAT'ATT
Cuts at: 948 948
Size: 4904
StyI C'CwwG_G
Cuts at: 107 932 1733 1769 2038 2392 107
Size: 825 801 36 269 354 2619
Fragments arranged by size:
2619 825 801 354 269 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 347 2487 2620 3079 3288 347
Size: 2140 133 459 209 1963
Fragments arranged by size:
2140 1963 459 209 133
TatI w'GTAC_w
Cuts at: 484 1363 1801 4419 484
Size: 879 438 2618 969
Fragments arranged by size:
2618 969 879 438
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4421 4453 4421
Size: 32 4872
Tth111I GACn'n_nGTC
Cuts at: 2701 2701
Size: 4904
XbaI T'CTAG_A
Cuts at: 1935 1935
Size: 4904
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1417 2399 1417
Size: 982 3922
XmaI C'CCGG_G
Cuts at: 742 1924 742
Size: 1182 3722
XmnI GAAnn'nnTTC
Cuts at: 626 626
Size: 4904
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PflMI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BmgBI BmtI Bpu10I
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI PacI PasI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI SanDI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI