Restriction map for pF1KB9517
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       Aor51HI     AscI        AseI        AsnI        
Asp718I     AspI        AviII       AvrII       BbvCI       
BcgI        BciVI       BfaI        BfrI        BglI        
BglII       BlnI        BseAI       BsmI        BspDI       
BspEI       BsrBI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
ClaI        CpoI        DraI        DrdI        EagI        
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
EcoT22I     FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MaeI        
MfeI        MluI        MroI        MseI        MunI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
SwaI        TfiI        Tru9I       Tth111I     XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccII         823  CGCG                 CG^CG
AcsI          907  GAATTC               [GA]^AATT[CT]
AcyI          542  GGCGCC               G[GA]^CG[CT]C
AgeI          977  ACCGGT               A^CCGGT
AhdI         1121  GACCCATCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI          907  GAATTC               [GA]^AATT[CT]
Asp700I       862  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1121  GACCCATCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          324  TGGCCA               TGG^CCA
BamHI         936  GGATCC               G^GATCC
BbeI          542  GGCGCC               GGCGC^C
BclI         1190  TGATCA               T^GATCA
BlpI          834  GCTCAGC              GC^T[ACGT]AGC
BpmI          145  CTCCAG               CTGGAG(16/14)
Bpu1102I      834  GCTCAGC              GC^T[ACGT]AGC
BsaBI         660  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         542  GGCGCC               G[GA]^CG[CT]C
BsaI           97  GGTCTC               GGTCTC(1/5)
BsaWI         977  ACCGGT               [AT]^CCGG[AT]
BsiEI        1136  CGGTCG               CG[GA][CT]^CG
BsiWI        1253  CGTACG               C^GTACG
BsmBI         357  CGTCTC               CGTCTC(1/5)
Bsp1407I     1103  TGTACA               T^GTACA
BsrDI        1518  CATTGC               GCAATG(2/0)
BsrGI        1103  TGTACA               T^GTACA
BstUI         823  CGCG                 CG^CG
CelII         834  GCTCAGC              GC^T[ACGT]AGC
DraIII       1417  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     1121  GACCCATCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          862  GAAGAG               CTCTTC(1/4)
EcoNI         894  CCTGCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         907  GAATTC               G^AATTC
EcoT14I       707  CCATGG               C^C[AT][AT]GG
FbaI         1190  TGATCA               T^GATCA
HgaI          372  GACGC                GACGC(5/10)
Hin1I         542  GGCGCC               G[GA]^CG[CT]C
KasI          542  GGCGCC               G^GCGCC
Ksp632I       862  GAAGAG               CTCTTC(1/4)
MamI          660  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         324  TGGCCA               TGG^CCA
MscI          324  TGGCCA               TGG^CCA
MvnI          823  CGCG                 CG^CG
NarI          542  GGCGCC               GG^CGCC
NcoI          707  CCATGG               C^CATGG
PaeR7I        221  CTCGAG               C^TCGAG
PflMI         395  CCACGGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         977  ACCGGT               A^CCGGT
PpuMI        1119  GGGACCC              [GA]G^G[AT]CC[CT]
SapI          862  GAAGAGC              GCTCTTC(1/4)
SexAI        1418  ACCTGGT              A^CC[AT]GGT
SfoI          542  GGCGCC               GGC^GCC
SspBI        1103  TGTACA               T^GTACA
StuI         1347  AGGCCT               AGG^CCT
StyI          707  CCATGG               C^C[AT][AT]GG
Tsp45I        336  GTCAC                ^GT[GC]AC
Van91I        395  CCACGGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          221  CTCGAG               C^TCGAG
XmnI          862  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AflIII         71  ACATGT               A^C[GA][CT]GT
              755  ACATGT              
Alw44I        759  GTGCAC               G^TGCAC
             1642  GTGCAC              
ApaLI         759  GTGCAC               G^TGCAC
             1642  GTGCAC              
AvaI          221  CTCGAG               C^[CT]CG[GA]G
              634  CCCGAG              
BbrPI         806  CACGTG               CAC^GTG
             1581  CACGTG              
BbsI         1386  GAAGAC               GAAGAC(2/6)
             1575  GAAGAC              
BpuAI        1386  GAAGAC               GAAGAC(2/6)
             1575  GAAGAC              
BsaAI         806  CACGTG               [CT]AC^GT[GA]
             1581  CACGTG              
BsoBI         221  CTCGAG               C^[CT]CG[GA]G
              634  CCCGAG              
BspLU11I       71  ACATGT               A^CATGT
              755  ACATGT              
BspMI         830  ACCTGC               ACCTGC(4/8)
             1245  GCAGGT              
BstYI         198  AGATCC               [GA]^GATC[CT]
              936  GGATCC              
EaeI          324  TGGCCA               [CT]^GGCC[GA]
             1234  TGGCCG              
FauI          821  CCCGC                CCCGC(4/6)
             1425  GCGGG               
MaeIII        336  GTCAC                ^GT[ACGT]AC
              472  GTTAC               
MflI          198  AGATCC               [GA]^GATC[CT]
              936  GGATCC              
PmaCI         806  CACGTG               CAC^GTG
             1581  CACGTG              
PmlI          806  CACGTG               CAC^GTG
             1581  CACGTG              
SfaNI         165  GATGC                GCATC(5/9)
             1529  GCATC               
SmlI          221  CTCGAG               C^T[CT][GA]AG
             1324  CTCAAG              
Tsp509I         4  AATT                 ^AATT
              908  AATT                
XcmI          431  CCAGTCTGCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              923  CCATGAACTTTGTGG     
XhoII         198  AGATCC               [GA]^GATC[CT]
              936  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AocI        ApaI        AspHI       AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspHI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        Eco81I      
EcoO109I    EcoRII      Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        PvuII       
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SfcI        TaqI        TseI        TspRI       TthHB8I     
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