Restriction map for pF1KB9517
 (Linear) (Six-base) MAPSORT of: KIBB9517.seq  Check: 6708  from: 1  to: 1695



KIBB9517.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1236    1695 
   Size:       1236     459
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158    1372    1594    1695 
   Size:        158    1214     222     101

  Fragments arranged by size: 

                1214     222     158     101
 
AflIII A'CryG_T

Cuts at:      0      71     755    1695 
   Size:         71     684     940

  Fragments arranged by size: 

                 940     684      71
 
AgeI A'CCGG_T

Cuts at:      0     977    1695 
   Size:        977     718
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1126    1695 
   Size:       1126     569
 
AleI CACnn'nnGTG

Cuts at:      0     481    1487    1695 
   Size:        481    1006     208

  Fragments arranged by size: 

                1006     481     208
 
AlwNI CAG_nnn'CTG

Cuts at:      0     288    1036    1532    1545    1695 
   Size:        288     748     496      13     150

  Fragments arranged by size: 

                 748     496     288     150      13
 
ApaI G_GGCC'C

Cuts at:      0      66    1404    1460    1695 
   Size:         66    1338      56     235

  Fragments arranged by size: 

                1338     235      66      56
 
ApaLI G'TGCA_C

Cuts at:      0     759    1642    1695 
   Size:        759     883      53

  Fragments arranged by size: 

                 883     759      53
 
ApoI r'AATT_y

Cuts at:      0     907    1695 
   Size:        907     788
 
AvaI C'yCGr_G

Cuts at:      0     221     634    1695 
   Size:        221     413    1061

  Fragments arranged by size: 

                1061     413     221
 
BamHI G'GATC_C

Cuts at:      0     936    1695 
   Size:        936     759
 
BanI G'GyrC_C

Cuts at:      0     488     542     630    1695 
   Size:        488      54      88    1065

  Fragments arranged by size: 

                1065     488      88      54
 
BanII G_rGCy'C

Cuts at:      0      66     146    1404    1460    1695 
   Size:         66      80    1258      56     235

  Fragments arranged by size: 

                1258     235      80      66      56
 
BbeI G_GCGC'C

Cuts at:      0     546    1695 
   Size:        546    1149
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1393    1582    1695 
   Size:       1393     189     113

  Fragments arranged by size: 

                1393     189     113
 
BclI T'GATC_A

Cuts at:      0    1190    1695 
   Size:       1190     505
 
BlpI GC'TnA_GC

Cuts at:      0     835    1695 
   Size:        835     860
 
Bme1580I G_kGCm'C

Cuts at:      0      66     635     763    1404    1460    1470    1646    1695 
   Size:         66     569     128     641      56      10     176      49

  Fragments arranged by size: 

                 641     569     176     128      66      56      49      10
            
 
BmgBI CAC'GTC

Cuts at:      0     419    1650    1695 
   Size:        419    1231      45

  Fragments arranged by size: 

                1231     419      45
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     425    1695 
   Size:        425    1270
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130    1695 
   Size:        130    1565
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1309    1695 
   Size:       1309     386
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     103    1695 
   Size:        103    1592
 
BsaAI yAC'GTr

Cuts at:      0     808    1583    1695 
   Size:        808     775     112

  Fragments arranged by size: 

                 808     775     112
 
BsaBI GATnn'nnATC

Cuts at:      0     664    1695 
   Size:        664    1031
 
BsaHI Gr'CG_yC

Cuts at:      0     543    1695 
   Size:        543    1152
 
BsaWI w'CCGG_w

Cuts at:      0     977    1695 
   Size:        977     718
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      83     113     343     373    1087    1117    1695 
   Size:         83      30     230      30     714      30     578

  Fragments arranged by size: 

                 714     578     230      83      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     170     351     607     738     793     826    1108    1219 
   Size:        170     181     256     131      55      33     282     111

Cuts at:   1219    1255    1354    1450    1695 
   Size:         36      99      96     245

  Fragments arranged by size: 

                 282     256     245     181     170     131     111      99
                  96      55      36      33
 
BseYI C'CCAG_C

Cuts at:      0     104     229    1445    1695 
   Size:        104     125    1216     250

  Fragments arranged by size: 

                1216     250     125     104
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1297    1399    1511    1695 
   Size:       1297     102     112     184

  Fragments arranged by size: 

                1297     184     112     102
 
BsiEI CG_ry'CG

Cuts at:      0    1139    1695 
   Size:       1139     556
 
BsiHKAI G_wGCw'C

Cuts at:      0     308     763    1172    1646    1695 
   Size:        308     455     409     474      49

  Fragments arranged by size: 

                 474     455     409     308      49
 
BsiWI C'GTAC_G

Cuts at:      0    1253    1695 
   Size:       1253     442
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     363    1695 
   Size:        363    1332
 
Bsp1286I G_dGCh'C

Cuts at:      0      66     146     308     635     763    1172    1404    1460 
   Size:         66      80     162     327     128     409     232      56

Cuts at:   1460    1470    1646    1695 
   Size:         10     176      49

  Fragments arranged by size: 

                 409     327     232     176     162     128      80      66
                  56      49      10
 
BspHI T'CATG_A

Cuts at:      0     870    1187    1652    1695 
   Size:        870     317     465      43

  Fragments arranged by size: 

                 870     465     317      43
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     839    1236    1695 
   Size:        839     397     459

  Fragments arranged by size: 

                 839     459     397
 
BsrDI GCAATG_nn'

Cuts at:      0    1517    1695 
   Size:       1517     178
 
BsrFI r'CCGG_y

Cuts at:      0     977    1304    1507    1695 
   Size:        977     327     203     188

  Fragments arranged by size: 

                 977     327     203     188
 
BsrGI T'GTAC_A

Cuts at:      0    1103    1695 
   Size:       1103     592
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     263    1422    1535    1695 
   Size:        263    1159     113     160

  Fragments arranged by size: 

                1159     263     160     113
 
BstYI r'GATC_y

Cuts at:      0     198     936    1695 
   Size:        198     738     759

  Fragments arranged by size: 

                 759     738     198
 
Bsu36I CC'TnA_GG

Cuts at:      0     496     533     674    1695 
   Size:        496      37     141    1021

  Fragments arranged by size: 

                1021     496     141      37
 
BtgI C'CryG_G

Cuts at:      0     395     455     707    1695 
   Size:        395      60     252     988

  Fragments arranged by size: 

                 988     395     252      60
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1092    1695 
   Size:       1092     603
 
BtsI GCAGTG_nn'

Cuts at:      0      87     523    1482    1494    1695 
   Size:         87     436     959      12     201

  Fragments arranged by size: 

                 959     436     201      87      12
 
DraIII CAC_nnn'GTG

Cuts at:      0    1422    1695 
   Size:       1422     273
 
EaeI y'GGCC_r

Cuts at:      0     324    1234    1695 
   Size:        324     910     461

  Fragments arranged by size: 

                 910     461     324
 
EarI CTCTTCn'nnn_

Cuts at:      0     857    1695 
   Size:        857     838
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     807     919    1695 
   Size:        807     112     776

  Fragments arranged by size: 

                 807     776     112
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     158    1372    1594    1695 
   Size:        130      28    1214     222     101

  Fragments arranged by size: 

                1214     222     130     101      28
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     898    1695 
   Size:        898     797
 
EcoO109I rG'GnC_Cy

Cuts at:      0      62      63     879    1120    1401    1456    1457    1695 
   Size:         62       1     816     241     281      55       1     238

  Fragments arranged by size: 

                 816     281     241     238      62      55       1       1
            
 
EcoRI G'AATT_C

Cuts at:      0     907    1695 
   Size:        907     788
 
HaeII r_GCGC'y

Cuts at:      0     230     546     842    1695 
   Size:        230     316     296     853

  Fragments arranged by size: 

                 853     316     296     230
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     673     705    1112    1144    1695 
   Size:        673      32     407      32     551

  Fragments arranged by size: 

                 673     551     407      32      32
 
KasI G'GCGC_C

Cuts at:      0     542    1695 
   Size:        542    1153
 
MscI TGG'CCA

Cuts at:      0     326    1695 
   Size:        326    1369
 
MslI CAynn'nnrTG

Cuts at:      0     481    1186    1487    1695 
   Size:        481     705     301     208

  Fragments arranged by size: 

                 705     481     301     208
 
MspA1I CmG'CkG

Cuts at:      0     317     387    1545    1674    1695 
   Size:        317      70    1158     129      21

  Fragments arranged by size: 

                1158     317     129      70      21
 
NarI GG'CG_CC

Cuts at:      0     543    1695 
   Size:        543    1152
 
NcoI C'CATG_G

Cuts at:      0     707    1695 
   Size:        707     988
 
NspI r_CATG'y

Cuts at:      0      75     759    1625    1695 
   Size:         75     684     866      70

  Fragments arranged by size: 

                 866     684      75      70
 
PasI CC'CwG_GG

Cuts at:      0     958    1695 
   Size:        958     737
 
PciI A'CATG_T

Cuts at:      0      71     755    1695 
   Size:         71     684     940

  Fragments arranged by size: 

                 940     684      71
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     401    1695 
   Size:        401    1294
 
PfoI T'CCnGG_A

Cuts at:      0     146     614    1695 
   Size:        146     468    1081

  Fragments arranged by size: 

                1081     468     146
 
PmlI CAC'GTG

Cuts at:      0     808    1583    1695 
   Size:        808     775     112

  Fragments arranged by size: 

                 808     775     112
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     714     746    1695 
   Size:        714      32     949

  Fragments arranged by size: 

                 949     714      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1120    1695 
   Size:       1120     575
 
PspOMI G'GGCC_C

Cuts at:      0      62    1400    1456    1695 
   Size:         62    1338      56     239

  Fragments arranged by size: 

                1338     239      62      56
 
PspXI vC'TCGA_Gb

Cuts at:      0     221    1695 
   Size:        221    1474
 
PstI C_TGCA'G

Cuts at:      0      82     719     902    1489    1695 
   Size:         82     637     183     587     206

  Fragments arranged by size: 

                 637     587     206     183      82
 
PvuII CAG'CTG

Cuts at:      0     387    1545    1674    1695 
   Size:        387    1158     129      21

  Fragments arranged by size: 

                1158     387     129      21
 
SanDI GG'GwC_CC

Cuts at:      0    1120    1695 
   Size:       1120     575
 
SapI GCTCTTCn'nnn_

Cuts at:      0     857    1695 
   Size:        857     838
 
SexAI A'CCwGG_T

Cuts at:      0    1418    1695 
   Size:       1418     277
 
SfcI C'TryA_G

Cuts at:      0      78     715     898    1485    1695 
   Size:         78     637     183     587     210

  Fragments arranged by size: 

                 637     587     210     183      78
 
SfoI GGC'GCC

Cuts at:      0     544    1695 
   Size:        544    1151
 
SmlI C'TyrA_G

Cuts at:      0     221    1324    1695 
   Size:        221    1103     371

  Fragments arranged by size: 

                1103     371     221
 
StuI AGG'CCT

Cuts at:      0    1349    1695 
   Size:       1349     346
 
StyI C'CwwG_G

Cuts at:      0     707    1695 
   Size:        707     988
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     531    1695 
   Size:        531    1164
 
TatI w'GTAC_w

Cuts at:      0    1103    1695 
   Size:       1103     592
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     438     930    1695 
   Size:        438     492     765

  Fragments arranged by size: 

                 765     492     438
 
XhoI C'TCGA_G

Cuts at:      0     221    1695 
   Size:        221    1474
 
XmnI GAAnn'nnTTC

Cuts at:      0     866    1695 
   Size:        866     829
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AgeI     AhdI     AleI    AlwNI     ApaI
    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
     BclI     BlpI Bme1580I    BmgBI     BmrI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI
    BsrFI    BsrGI   BstAPI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI
    HaeII    Hin4I     KasI     MscI     MslI   MspA1I     NarI     NcoI
     NspI     PasI     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI
   PspOMI    PspXI     PstI    PvuII    SanDI     SapI    SexAI     SfcI
     SfoI     SmlI     StuI     StyI    TaqII     TatI     XcmI     XhoI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AlfI     AloI
     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII     BmtI     BplI   Bpu10I     BsmI    BspEI
    BsrBI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I     ClaI
    CspCI     DraI     DrdI     EagI  EcoICRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PmeI    PshAI     PsiI     PsrI     PvuI    RsrII     SacI
    SacII     SalI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI  Tth111I
     XbaI     XmaI     ZraI
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