Restriction map for
pF1KB9505
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# NO CUT ENZYMES ( 174 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AflII AgeI AhdI AluI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspDI
BspEI BspHI BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DpnII
DraI DraII DraIII DrdI DsaI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT14I FbaI FseI FspI HaeII
HgaI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeIII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MunI MvaI MvnI NarI NcoI
NdeII NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI StyI SwaI
TaqI Tsp45I Tth111I TthHB8I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AatII 96 GACGTC GACGT^C
AcyI 96 GACGTC G[GA]^CG[CT]C
AfaI 613 GTAC GT^AC
AflIII 420 ACATGT A^C[GA][CT]GT
AlwNI 452 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 511 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 253 GGTCC G^G[AT]CC
BbsI 93 GAAGAC GAAGAC(2/6)
BciVI 219 GGATAC GTATCC(6/5)
BcnI 592 CCGGG CC^[GC]GG
BfaI 584 CTAG C^TAG
BpuAI 93 GAAGAC GAAGAC(2/6)
BsaHI 96 GACGTC G[GA]^CG[CT]C
BsiEI 8 CGGCCG CG[GA][CT]^CG
BsiYI 243 CCATGCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 243 CCATGCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 417 GAGAC GTCTC(1/5)
Bsp1407I 612 TGTACA T^GTACA
BspLU11I 420 ACATGT A^CATGT
BspMI 226 GCAGGT ACCTGC(4/8)
BsrDI 575 GCAATG GCAATG(2/0)
BsrFI 10 GCCGGC [GA]^CCGG[CT]
BsrGI 612 TGTACA T^GTACA
BssKI 592 CCGGG ^CC[ACGT]GG
BstXI 642 CCAGACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 111 GCGC GCG^C
Cfr10I 10 GCCGGC [GA]^CCGG[CT]
EaeI 8 CGGCCG [CT]^GGCC[GA]
EagI 8 CGGCCG C^GGCCG
EclXI 8 CGGCCG C^GGCCG
Eco52I 8 CGGCCG C^GGCCG
EcoT22I 245 ATGCAT ATGCA^T
HhaI 111 GCGC GCG^C
Hin1I 96 GACGTC G[GA]^CG[CT]C
HinP1I 111 GCGC G^CGC
HphI 146 TCACC GGTGA(8/7)
MaeI 584 CTAG C^TAG
MaeII 97 ACGT A^CGT
NaeI 10 GCCGGC GCC^GGC
NciI 592 CCGGG CC^[GC]GG
NdeI 213 CATATG CA^TATG
NgoMIV 10 GCCGGC G^CCGGC
NlaIV 252 GGGTCC GG[ACGT]^[ACGT]CC
NsiI 245 ATGCAT ATGCA^T
NspI 420 ACATGT [GA]CATG^[CT]
PflMI 243 CCATGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 442 GAGTC GAGTC(4/5)
PstI 154 CTGCAG CTGCA^G
RsaI 613 GTAC GT^AC
ScrFI 592 CCGGG CC^[ACGT]GG
SfaNI 160 GCATC GCATC(5/9)
SmlI 635 CTCAAG C^T[CT][GA]AG
SspBI 612 TGTACA T^GTACA
SspI 184 AATATT AAT^ATT
TspRI 648 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 243 CCATGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 642 CCAGACCAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 511 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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BsrI 335 ACTGG ACTGG(1/-1)
647 CCAGT
BstF5I 536 GGATG GGATG(2/0)
558 GGATG
Cfr13I 42 GGGCC G^G[ACGT]CC
253 GGTCC
FauI 26 GCGGG CCCGC(4/6)
40 GCGGG
FokI 536 GGATG GGATG(9/13)
558 GGATG
HaeIII 9 GGCC GG^CC
43 GGCC
HapII 11 CCGG C^CGG
592 CCGG
HpaII 11 CCGG C^CGG
592 CCGG
MspI 11 CCGG C^CGG
592 CCGG
NlaIII 244 CATG CATG^
421 CATG
Sau96I 42 GGGCC G^G[ACGT]CC
253 GGTCC
SfcI 154 CTGCAG C^T[GA][CT]AG
264 CTACAG
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
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AciI BbvI Cac8I Fnu4HI HinfI
ItaI MboII MnlI MseI MwoI
TfiI Tru9I TseI Tsp509I