Restriction map for
pF1KB9505
(Circular) (Six-base) MAPSORT of: pF1KB9505.seq Check: 6941 from: 1 to: 3788
pF1KB9505 3788 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AatII G_ACGT'C
Cuts at: 208 208
Size: 3788
AccI GT'mk_AC
Cuts at: 826 826
Size: 3788
Acc65I G'GTAC_C
Cuts at: 804 804
Size: 3788
AclI AA'CG_TT
Cuts at: 3628 3628
Size: 3788
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 575 1613 2045 2792 3060 575
Size: 1038 432 747 268 1303
Fragments arranged by size:
1303 1038 747 432 268
AflIII A'CryG_T
Cuts at: 528 1140 2244 3221 528
Size: 612 1104 977 1095
Fragments arranged by size:
1104 1095 977 612
AgeI A'CCGG_T
Cuts at: 2176 2176
Size: 3788
AlwNI CAG_nnn'CTG
Cuts at: 565 1093 2660 565
Size: 528 1567 1693
Fragments arranged by size:
1693 1567 528
ApaLI G'TGCA_C
Cuts at: 2558 2558
Size: 3788
ApoI r'AATT_y
Cuts at: 792 3141 3397 792
Size: 2349 256 1183
Fragments arranged by size:
2349 1183 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3788
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
AvaI C'yCGr_G
Cuts at: 808 808
Size: 3788
BamHI G'GATC_C
Cuts at: 813 813
Size: 3788
BanI G'GyrC_C
Cuts at: 804 1218 1466 1501 804
Size: 414 248 35 3091
Fragments arranged by size:
3091 414 248 35
BanII G_rGCy'C
Cuts at: 802 1832 802
Size: 1030 2758
BbeI G_GCGC'C
Cuts at: 1222 1470 1222
Size: 248 3540
BbsI GAAGACnn'nnnn_
Cuts at: 208 208
Size: 3788
BciVI GTATCCnnnnn_n'
Cuts at: 321 1686 2447 3788 321
Size: 1365 761 1341 321
Fragments arranged by size:
1365 1341 761 321
BclI T'GATC_A
Cuts at: 1308 1308
Size: 3788
BfrBI ATG'CAT
Cuts at: 355 355
Size: 3788
BglII A'GATC_T
Cuts at: 1303 3340 1303
Size: 2037 1751
BlpI GC'TnA_GC
Cuts at: 900 900
Size: 3788
Bme1580I G_kGCm'C
Cuts at: 1413 1506 2562 1413
Size: 93 1056 2639
Fragments arranged by size:
2639 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 453 1000 1081 1416 3560 453
Size: 547 81 335 2144 681
Fragments arranged by size:
2144 681 547 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 3788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 800
Size: 3788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 728 964 1843 2335 2633 728
Size: 236 879 492 298 1883
Fragments arranged by size:
1883 879 492 298 236
BsaI GGTCTCn'nnnn_
Cuts at: 3492 3492
Size: 3788
BsaAI yAC'GTr
Cuts at: 1141 1141
Size: 3788
BsaBI GATnn'nnATC
Cuts at: 6 1302 3339 6
Size: 1296 2037 455
Fragments arranged by size:
2037 1296 455
BsaHI Gr'CG_yC
Cuts at: 205 1219 1467 2169 3665 205
Size: 1014 248 702 1496 328
Fragments arranged by size:
1496 1014 702 328 248
BsaWI w'CCGG_w
Cuts at: 977 1498 2176 2450 2597 3008 977
Size: 521 678 274 147 411 1757
Fragments arranged by size:
1757 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 180 1213 1971 2548 3044 180
Size: 1033 758 577 496 924
Fragments arranged by size:
1033 924 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 119 1330 1376 2584 104
Size: 15 1211 46 1208 1308
Fragments arranged by size:
1308 1211 1208 46 15
BsiHKAI G_wGCw'C
Cuts at: 802 1580 1770 2562 802
Size: 778 190 792 2028
Fragments arranged by size:
2028 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 802 1413 1506 1580 1770 1832 2562 802
Size: 611 93 74 190 62 730 2028
Fragments arranged by size:
2028 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3008 3008
Size: 3788
BspHI T'CATG_A
Cuts at: 2964 2964
Size: 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 325 838 1052 1354 1735 325
Size: 513 214 302 381 2378
Fragments arranged by size:
2378 513 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2080 2134 3618 3783 50
Size: 2030 54 1484 165 55
Fragments arranged by size:
2030 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 690 1700 690
Size: 1010 2778
BsrFI r'CCGG_y
Cuts at: 118 1786 1967 2176 118
Size: 1668 181 209 1730
Fragments arranged by size:
1730 1668 209 181
BsrGI T'GTAC_A
Cuts at: 720 720
Size: 3788
BssSI C'ACGA_G
Cuts at: 2059 2417 2059
Size: 358 3430
BstAPI GCAn_nnn'nTGC
Cuts at: 1165 1165
Size: 3788
BstBI TT'CG_AA
Cuts at: 2149 2149
Size: 3788
BstXI CCAn_nnnn'nTGG
Cuts at: 757 757
Size: 3788
BstYI r'GATC_y
Cuts at: 1 813 1280 1303 1638 1884 2896 2982 2994
Size: 812 467 23 335 246 1012 86 12
Cuts at: 2994 3340 1
Size: 346 449
Fragments arranged by size:
1012 812 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3788
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1738 1919 2082 1738
Size: 181 163 3444
Fragments arranged by size:
3444 181 163
BtsI GCAGTG_nn'
Cuts at: 1031 1031
Size: 3788
DraI TTT'AAA
Cuts at: 787 787
Size: 3788
DrdI GACnn_nn'nnGTC
Cuts at: 1494 2352 1494
Size: 858 2930
EaeI y'GGCC_r
Cuts at: 116 1373 1547 1938 1965 3310 116
Size: 1257 174 391 27 1345 594
Fragments arranged by size:
1345 1257 594 391 174 27
EagI C'GGCC_G
Cuts at: 116 1373 116
Size: 1257 2531
EarI CTCTTCn'nnn_
Cuts at: 1811 2021 1811
Size: 210 3578
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1996 2306 2452 3598 1996
Size: 310 146 1146 2186
Fragments arranged by size:
2186 1146 310 146
EcoICRI GAG'CTC
Cuts at: 800 800
Size: 3788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 575 800 1613 2045 2792 3060 575
Size: 225 813 432 747 268 1303
Fragments arranged by size:
1303 813 747 432 268 225
EcoO109I rG'GnC_Cy
Cuts at: 927 927
Size: 3788
EcoRI G'AATT_C
Cuts at: 792 792
Size: 3788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1807 1807
Size: 3788
FspI TGC'GCA
Cuts at: 1569 1569
Size: 3788
HaeII r_GCGC'y
Cuts at: 1222 1470 2492 3471 1222
Size: 248 1022 979 1539
Fragments arranged by size:
1539 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1630 1660 1662 1692 1630
Size: 30 2 30 3726
Fragments arranged by size:
3726 30 30 2
HincII GTy'rAC
Cuts at: 827 827
Size: 3788
KasI G'GCGC_C
Cuts at: 1218 1466 1218
Size: 248 3540
KpnI G_GTAC'C
Cuts at: 808 808
Size: 3788
MluI A'CGCG_T
Cuts at: 3221 3221
Size: 3788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2459 2643 2459
Size: 184 3604
MscI TGG'CCA
Cuts at: 1549 1549
Size: 3788
MslI CAynn'nnrTG
Cuts at: 1777 1904 1777
Size: 127 3661
MspA1I CmG'CkG
Cuts at: 899 1213 1573 2586 2831 899
Size: 314 360 1013 245 1856
Fragments arranged by size:
1856 1013 360 314 245
NaeI GCC'GGC
Cuts at: 120 1969 120
Size: 1849 1939
NarI GG'CG_CC
Cuts at: 1219 1467 1219
Size: 248 3540
NcoI C'CATG_G
Cuts at: 107 107
Size: 3788
NdeI CA'TA_TG
Cuts at: 322 322
Size: 3788
NgoMIV G'CCGG_C
Cuts at: 118 1967 118
Size: 1849 1939
NsiI A_TGCA'T
Cuts at: 357 357
Size: 3788
NspI r_CATG'y
Cuts at: 532 841 1341 2248 532
Size: 309 500 907 2072
Fragments arranged by size:
2072 907 500 309
PciI A'CATG_T
Cuts at: 528 2244 528
Size: 1716 2072
PflMI CCAn_nnn'nTGG
Cuts at: 357 357
Size: 3788
PmeI GTTT'AAAC
Cuts at: 787 787
Size: 3788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2927 2959 2927
Size: 32 3756
PstI C_TGCA'G
Cuts at: 266 835 1520 266
Size: 569 685 2534
Fragments arranged by size:
2534 685 569
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3788
PvuII CAG'CTG
Cuts at: 1213 1573 1213
Size: 360 3428
RsrII CG'GwC_CG
Cuts at: 1983 1983
Size: 3788
SacI G_AGCT'C
Cuts at: 802 802
Size: 3788
SalI G'TCGA_C
Cuts at: 825 825
Size: 3788
SapI GCTCTTCn'nnn_
Cuts at: 1811 2021 1811
Size: 210 3578
SbfI CC_TGCA'GG
Cuts at: 835 835
Size: 3788
ScaI AGT'ACT
Cuts at: 3305 3305
Size: 3788
SfcI C'TryA_G
Cuts at: 33 262 372 831 1516 2509 2700 33
Size: 229 110 459 685 993 191 1121
Fragments arranged by size:
1121 993 685 459 229 191 110
SfoI GGC'GCC
Cuts at: 1220 1468 1220
Size: 248 3540
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
SmaI CCC'GGG
Cuts at: 810 810
Size: 3788
SmlI C'TyrA_G
Cuts at: 743 943 1858 2350 2612 743
Size: 200 915 492 262 1919
Fragments arranged by size:
1919 915 492 262 200
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3788
SphI G_CATG'C
Cuts at: 841 1341 841
Size: 500 3288
SspI AAT'ATT
Cuts at: 294 294
Size: 3788
StyI C'CwwG_G
Cuts at: 107 922 1276 107
Size: 815 354 2619
Fragments arranged by size:
2619 815 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1371 1504 1963 2172 1371
Size: 133 459 209 2987
Fragments arranged by size:
2987 459 209 133
TatI w'GTAC_w
Cuts at: 720 3303 720
Size: 2583 1205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 3756
Tth111I GACn'n_nGTC
Cuts at: 1585 1585
Size: 3788
XbaI T'CTAG_A
Cuts at: 819 819
Size: 3788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 757 1283 757
Size: 526 3262
XmaI C'CCGG_G
Cuts at: 808 808
Size: 3788
XmnI GAAnn'nnTTC
Cuts at: 623 623
Size: 3788
ZraI GAC'GTC
Cuts at: 206 206
Size: 3788
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI