Restriction map for pF1KB9500
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       AccI        AclI        AcyI        AflIII      
AgeI        AlwNI       AocI        Aor51HI     ApaI        
AscI        AvaI        AviII       AvrII       BamHI       
BbeI        BbrPI       BciVI       BglI        BlnI        
BsaAI       BsaBI       BsaHI       BsgI        BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspLU11I    BspMI       BsrBI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CfoI        ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRV       FauI        FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HpaI        KasI        KspI        MamI        MluI        
NaeI        NarI        NcoI        NgoMIV      NheI        
NotI        NruI        NspV        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SwaI        Van91I      XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

Acc65I       2004  GGTACC               G^GTACC
AccII        1595  CGCG                 CG^CG
AccIII       1754  TCCGGA               T^CCGGA
AflII         259  CTTAAG               C^TTAAG
AhdI          955  GACTTGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        158  GTGCAC               G^TGCAC
AlwI         1156  GATCC                GGATC(4/5)
ApaLI         158  GTGCAC               G^TGCAC
AseI          910  ATTAAT               AT^TAAT
AsnI          910  ATTAAT               AT^TAAT
Asp700I      1807  GAAAGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2004  GGTACC               G^GTACC
AspEI         955  GACTTGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         158  GTGCAC               G[AT]GC[AT]^C
AspI          826  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanII         703  GAGCCC               G[GA]GC[CT]^C
BbvCI        2019  GCTGAGG              CCTCAGC(-5/-2)
BcgI          672  CGATAAGGTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          259  CTTAAG               C^TTAAG
BlpI          682  GCTGAGC              GC^T[ACGT]AGC
BpmI          119  CTGGAG               CTGGAG(16/14)
Bpu1102I      682  GCTGAGC              GC^T[ACGT]AGC
BseAI        1754  TCCGGA               T^CCGGA
BsiHKAI       158  GTGCAC               G[AT]GC[AT]^C
BsmFI        1187  GGGAC                GGGAC(10/14)
BspEI        1754  TCCGGA               T^CCGGA
BspHI        1496  TCATGA               T^CATGA
BsrDI        1788  CATTGC               GCAATG(2/0)
BsrFI        1570  GCCGGT               [GA]^CCGG[CT]
BssSI         785  CTCGTG               CACGAG(-5/-1)
BstAPI        687  GCAGGAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1595  CGCG                 CG^CG
CelII         682  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1570  GCCGGT               [GA]^CCGG[CT]
Eam1105I      955  GACTTGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I      1028  CCAAGG               C^C[AT][AT]GG
HgaI         1485  GCGTC                GACGC(5/10)
HindIII      1230  AAGCTT               A^AGCTT
KpnI         2004  GGTACC               GGTAC^C
MaeII         150  ACGT                 A^CGT
MfeI         1925  CAATTG               C^AATTG
MroI         1754  TCCGGA               T^CCGGA
MspA1I       1622  CAGCTG               C[AC]G^C[GT]G
MunI         1925  CAATTG               C^AATTG
MvnI         1595  CGCG                 CG^CG
NdeI           13  CATATG               CA^TATG
NspI           24  GCATGT               [GA]CATG^[CT]
PacI         1972  TTAATTAA             TTAAT^TAA
PflFI         826  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI         984  GACTTTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         910  ATTAAT               AT^TAAT
PvuII        1622  CAGCTG               CAG^CTG
RcaI         1496  TCATGA               T^CATGA
SspI          360  AATATT               AAT^ATT
StuI         1172  AGGCCT               AGG^CCT
StyI         1028  CCAAGG               C^C[AT][AT]GG
Tsp45I       1407  GTCAC                ^GT[GC]AC
Tth111I       826  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          903  TCTAGA               T^CTAGA
XmnI         1807  GAAAGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AvaII        1456  GGTCC                G^G[AT]CC
             1512  GGACC               
BanI          130  GGCACC               G^G[CT][GA]CC
             2004  GGTACC              
BclI         1142  TGATCA               T^GATCA
             1697  TGATCA              
BcnI         1211  CCGGG                CC^[GC]GG
             1544  CCCGG               
BglII         561  AGATCT               A^GATCT
             1385  AGATCT              
BsaI         1437  GAGACC               GGTCTC(1/5)
             1556  GAGACC              
BsaWI         289  ACCGGA               [AT]^CCGG[AT]
             1754  TCCGGA              
BseRI         749  GAGGAG               GAGGAG(10/8)
             1381  GAGGAG              
BsmI          480  GAATGC               GAATGC(1/-1)
             1603  GCATTC              
EcoRI        1758  GAATTC               G^AATTC
             1932  GAATTC              
EcoT22I       482  ATGCAT               ATGCA^T
              970  ATGCAT              
FbaI         1142  TGATCA               T^GATCA
             1697  TGATCA              
HincII        109  GTTGAC               GT[CT]^[GA]AC
             1004  GTCAAC              
HindII        109  GTTGAC               GT[CT]^[GA]AC
             1004  GTCAAC              
MaeIII         28  GTTAC                ^GT[ACGT]AC
             1407  GTCAC               
MslI           13  CATATGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              132  CACCAGGATG          
NciI         1211  CCGGG                CC^[GC]GG
             1544  CCCGG               
NsiI          482  ATGCAT               ATGCA^T
              970  ATGCAT              
PleI          826  GACTC                GAGTC(4/5)
             1749  GACTC               
PstI          863  CTGCAG               CTGCA^G
             1526  CTGCAG              
SmlI          259  CTTAAG               C^T[CT][GA]AG
              569  CTCAAG              
TspRI         443  CACTG                CA[GC]TG[ACGT][ACGT]^
             1999  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BalI        BbsI        BbvI        BfaI        BmyI        
BpuAI       BsaJI       BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       EaeI        EarI        EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TaqI        TfiI        
Tru9I       TseI        Tsp509I     TthHB8I     XhoII       
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