Restriction map for pF1KB9484
 (Linear) (Six-base) MAPSORT of: KIBB9484.seq  Check: 5144  from: 1  to: 6135



KIBB9484.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    4069    5409    5789    5889    6092    6135 
   Size:       4069    1340     380     100     203      43

  Fragments arranged by size: 

                4069    1340     380     203     100      43
 
AatII G_ACGT'C

Cuts at:      0    3545    3963    4574    5429    6135 
   Size:       3545     418     611     855     706

  Fragments arranged by size: 

                3545     855     706     611     418
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2103    2212    2869    3624    5295    5296    6135 
   Size:       2103     109     657     755    1671       1     839

  Fragments arranged by size: 

                2103    1671     839     755     657     109       1
 
AflIII A'CryG_T

Cuts at:      0     185    1432    1715    3919    6135 
   Size:        185    1247     283    2204    2216

  Fragments arranged by size: 

                2216    2204    1247     283     185
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3471    5070    5430    6135 
   Size:       3471    1599     360     705

  Fragments arranged by size: 

                3471    1599     705     360
 
AleI CACnn'nnGTG

Cuts at:      0     487    3394    6135 
   Size:        487    2907    2741

  Fragments arranged by size: 

                2907    2741     487
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     721    1367    2004    4579    4976    6135 
   Size:        721     646     637    2575     397    1159

  Fragments arranged by size: 

                2575    1159     721     646     637     397
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     433     465    4984    5016    6135 
   Size:        433      32    4519      32    1119

  Fragments arranged by size: 

                4519    1119     433      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     773     894    1925    2055    2375    2874    2895    2988 
   Size:        773     121    1031     130     320     499      21      93

Cuts at:   2988    3441    4221    4587    4698    5394    5712    6135 
   Size:        453     780     366     111     696     318     423

  Fragments arranged by size: 

                1031     780     773     696     499     453     423     366
                 320     318     130     121     111      93      21
 
ApaI G_GGCC'C

Cuts at:      0      25    1378    2662    2902    3587    3666    3864    4686 
   Size:         25    1353    1284     240     685      79     198     822

Cuts at:   4686    4904    6007    6135 
   Size:        218    1103     128

  Fragments arranged by size: 

                1353    1284    1103     822     685     240     218     198
                 128      79      25
 
ApaLI G'TGCA_C

Cuts at:      0    1744    3616    6135 
   Size:       1744    1872    2519

  Fragments arranged by size: 

                2519    1872    1744
 
ApoI r'AATT_y

Cuts at:      0    3534    4195    6135 
   Size:       3534     661    1940

  Fragments arranged by size: 

                3534    1940     661
 
AvaI C'yCGr_G

Cuts at:      0      18     242     469     838     978    1036    1127    1405 
   Size:         18     224     227     369     140      58      91     278

Cuts at:   1405    1482    1564    1976    2342    2608    3253    3454    3659 
   Size:         77      82     412     366     266     645     201     205

Cuts at:   3659    3857    4244    4495    4619    4819    4881    5456    5499 
   Size:        198     387     251     124     200      62     575      43

Cuts at:   5499    5530    6135 
   Size:         31     605

  Fragments arranged by size: 

                 645     605     575     412     387     369     366     278
                 266     251     227     224     205     201     200     198
                 140     124      91      82      77      62      58      43
                  31      18
 
BanI G'GyrC_C

Cuts at:      0     538     618     832     865     964    1013    1059    2407 
   Size:        538      80     214      33      99      49      46    1348

Cuts at:   2407    2557    3004    3120    3132    3645    3692    4087    4111 
   Size:        150     447     116      12     513      47     395      24

Cuts at:   4111    4361    4388    4396    4447    4537    4800    4861    5206 
   Size:        250      27       8      51      90     263      61     345

Cuts at:   5206    5356    5777    5941    6071    6135 
   Size:        150     421     164     130      64

  Fragments arranged by size: 

                1348     538     513     447     421     395     345     263
                 250     214     164     150     150     130     116      99
                  90      80      64      61      51      49      47      46
                  33      27      24      12       8
 
BanII G_rGCy'C

Cuts at:      0      25     554     601    1193    1378    1632    2225    2662 
   Size:         25     529      47     592     185     254     593     437

Cuts at:   2662    2902    2972    3254    3260    3376    3587    3666    3864 
   Size:        240      70     282       6     116     211      79     198

Cuts at:   3864    4686    4771    4904    5806    5970    6007    6135 
   Size:        822      85     133     902     164      37     128

  Fragments arranged by size: 

                 902     822     593     592     529     437     282     254
                 240     211     198     185     164     133     128     116
                  85      79      70      47      37      25       6
 
BbeI G_GCGC'C

Cuts at:      0     542     622     836     869     968    1017    1063    2561 
   Size:        542      80     214      33      99      49      46    1498

Cuts at:   2561    3008    3696    4091    4115    4392    4541    4804    5945 
   Size:        447     688     395      24     277     149     263    1141

Cuts at:   5945    6135 
   Size:        190

  Fragments arranged by size: 

                1498    1141     688     542     447     395     277     263
                 214     190     149      99      80      49      46      33
                  24
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     918    2857    3355    5053    6135 
   Size:        918    1939     498    1698    1082

  Fragments arranged by size: 

                1939    1698    1082     918     498
 
BbvCI CC'TCA_GC

Cuts at:      0    1782    6135 
   Size:       1782    4353
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     117     151    1163    1197    4810    4844    6135 
   Size:        117      34    1012      34    3613      34    1291

  Fragments arranged by size: 

                3613    1291    1012     117      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    5586    6135 
   Size:       5586     549
 
BclI T'GATC_A

Cuts at:      0     278    6135 
   Size:        278    5857
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1057    2360    2525    2666    2870    3643    4076    4218 
   Size:       1057    1303     165     141     204     773     433     142

Cuts at:   4218    4733    5021    6135 
   Size:        515     288    1114

  Fragments arranged by size: 

                1303    1114    1057     773     515     433     288     204
                 165     142     141
 
BlpI GC'TnA_GC

Cuts at:      0     709    2751    6135 
   Size:        709    2042    3384

  Fragments arranged by size: 

                3384    2042     709
 
Bme1580I G_kGCm'C

Cuts at:      0      25     607     817    1186    1290    1378    1666    1715 
   Size:         25     582     210     369     104      88     288      49

Cuts at:   1715    1748    2301    2655    2662    2902    3125    3587    3620 
   Size:         33     553     354       7     240     223     462      33

Cuts at:   3620    3648    3666    3864    3956    4399    4686    4729    4846 
   Size:         28      18     198      92     443     287      43     117

Cuts at:   4846    4864    4904    5211    5780    5837    6007    6135 
   Size:         18      40     307     569      57     170     128

  Fragments arranged by size: 

                 582     569     553     462     443     369     354     307
                 288     287     240     223     210     198     170     128
                 117     104      92      88      57      49      43      40
                  33      33      28      25      18      18       7
 
BmgBI CAC'GTC

Cuts at:      0    1433    3654    3782    3922    5432    5589    6135 
   Size:       1433    2221     128     140    1510     157     546

  Fragments arranged by size: 

                2221    1510    1433     546     157     140     128
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     335    2363    3866    4047    4436    6135 
   Size:        335    2028    1503     181     389    1699

  Fragments arranged by size: 

                2028    1699    1503     389     335     181
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     316    2766    4155    6135 
   Size:        316    2450    1389    1980

  Fragments arranged by size: 

                2450    1980    1389     316
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     390     457     470    1564    1936    3433    3439 
   Size:        127     263      67      13    1094     372    1497       6

Cuts at:   3439    4228    4390    4540    4609    4720    4957    5011    5119 
   Size:        789     162     150      69     111     237      54     108

Cuts at:   5119    5206    5742    6109    6135 
   Size:         87     536     367      26

  Fragments arranged by size: 

                1497    1094     789     536     372     367     263     237
                 162     150     127     111     108      87      69      67
                  54      26      13       6
 
Bpu10I CC'TnA_GC

Cuts at:      0    1782    2977    4294    6135 
   Size:       1782    1195    1317    1841

  Fragments arranged by size: 

                1841    1782    1317    1195
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     287    1138    1169    1639    1792    4411    5184    5376 
   Size:        287     851      31     470     153    2619     773     192

Cuts at:   5376    6135 
   Size:        759

  Fragments arranged by size: 

                2619     851     773     759     470     287     192     153
                  31
 
BsaAI yAC'GTr

Cuts at:      0     188    1304    1718    1743    1776    2229    3732    5292 
   Size:        188    1116     414      25      33     453    1503    1560

Cuts at:   5292    5409    6135 
   Size:        117     726

  Fragments arranged by size: 

                1560    1503    1116     726     453     414     188     117
                  33      25
 
BsaHI Gr'CG_yC

Cuts at:      0     499     539     577     619     638     833     866     965 
   Size:        499      40      38      42      19     195      33      99

Cuts at:    965    1014    1060    1517    2305    2558    3005    3160    3352 
   Size:         49      46     457     788     253     447     155     192

Cuts at:   3352    3407    3425    3542    3693    3960    4088    4112    4389 
   Size:         55      18     117     151     267     128      24     277

Cuts at:   4389    4538    4571    4801    5426    5942    6095    6135 
   Size:        149      33     230     625     516     153      40

  Fragments arranged by size: 

                 788     625     516     499     457     447     277     267
                 253     230     195     192     155     153     151     149
                 128     117      99      55      49      46      42      40
                  40      38      33      33      24      19      18
 
BsaWI w'CCGG_w

Cuts at:      0    2011    3530    3557    6135 
   Size:       2011    1519      27    2578

  Fragments arranged by size: 

                2578    2011    1519      27
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     175     205     433     463     727     757    1799    1829 
   Size:        175      30     228      30     264      30    1042      30

Cuts at:   1829    2514    2544    3036    3066    4076    4106    4912    4942 
   Size:        685      30     492      30    1010      30     806      30

Cuts at:   4942    4984    5014    5788    5818    6135 
   Size:         42      30     774      30     317

  Fragments arranged by size: 

                1042    1010     806     774     685     492     317     264
                 228     175      42      30      30      30      30      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     133     136     169     454    1126    1984    2755    2962 
   Size:        133       3      33     285     672     858     771     207

Cuts at:   2962    5919    6135 
   Size:       2957     216

  Fragments arranged by size: 

                2957     858     771     672     285     216     207     133
                  33       3
 
BseYI C'CCAG_C

Cuts at:      0     607    1360    1688    1723    2418    2923    3022    3148 
   Size:        607     753     328      35     695     505      99     126

Cuts at:   3148    3943    3977    5017    5807    5937    6006    6135 
   Size:        795      34    1040     790     130      69     129

  Fragments arranged by size: 

                1040     795     790     753     695     607     505     328
                 130     129     126      99      69      35      34
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     341     702    1432    1723    1737    1756    3128 
   Size:        199     142     361     730     291      14      19    1372

Cuts at:   3128    4309    4936    5436    6064    6135 
   Size:       1181     627     500     628      71

  Fragments arranged by size: 

                1372    1181     730     628     627     500     361     291
                 199     142      71      19      14
 
BsiEI CG_ry'CG

Cuts at:      0       9      34     788    1117    1251    2649    3718    4737 
   Size:          9      25     754     329     134    1398    1069    1019

Cuts at:   4737    5199    6135 
   Size:        462     936

  Fragments arranged by size: 

                1398    1069    1019     936     754     462     329     134
                  25       9
 
BsiHKAI G_wGCw'C

Cuts at:      0     140    1483    1748    1840    1874    2437    2599    3514 
   Size:        140    1343     265      92      34     563     162     915

Cuts at:   3514    3620    3731    4833    5408    5720    6135 
   Size:        106     111    1102     575     312     415

  Fragments arranged by size: 

                1343    1102     915     575     563     415     312     265
                 162     140     111     106      92      34
 
BsmI GAATG_Cn'

Cuts at:      0     527     664    1471    1678    6135 
   Size:        527     137     807     207    4457

  Fragments arranged by size: 

                4457     807     527     207     137
 
Bsp1286I G_dGCh'C

Cuts at:      0      25     140     554     601     607     817    1186    1193 
   Size:         25     115     414      47       6     210     369       7

Cuts at:   1193    1290    1378    1483    1632    1666    1715    1748    1840 
   Size:         97      88     105     149      34      49      33      92

Cuts at:   1840    1874    2225    2301    2437    2599    2655    2662    2902 
   Size:         34     351      76     136     162      56       7     240

Cuts at:   2902    2972    3125    3254    3260    3376    3514    3587    3620 
   Size:         70     153     129       6     116     138      73      33

Cuts at:   3620    3648    3666    3731    3864    3956    4399    4686    4729 
   Size:         28      18      65     133      92     443     287      43

Cuts at:   4729    4771    4833    4846    4864    4904    5211    5408    5720 
   Size:         42      62      13      18      40     307     197     312

Cuts at:   5720    5780    5806    5837    5970    6007    6135 
   Size:         60      26      31     133      37     128

  Fragments arranged by size: 

                 443     414     369     351     312     307     287     240
                 210     197     162     153     149     138     136     133
                 133     129     128     116     115     105      97      92
                  92      88      76      73      70      65      62      60
                  56      49      47      43      42      40      37      34
                  34      33      33      31      28      26      25      18
                  18      13       7       7       6       6
 
BspEI T'CCGG_A

Cuts at:      0    2011    3530    3557    6135 
   Size:       2011    1519      27    2578

  Fragments arranged by size: 

                2578    2011    1519      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     763     832    4036    4069    5409    5431    5789    5889 
   Size:        763      69    3204      33    1340      22     358     100

Cuts at:   5889    6092    6135 
   Size:        203      43

  Fragments arranged by size: 

                3204    1340     763     358     203     100      69      43
                  33      22
 
BsrBI CCG'CTC

Cuts at:      0      45     111    3215    4139    4524    5286    5742    6135 
   Size:         45      66    3104     924     385     762     456     393

  Fragments arranged by size: 

                3104     924     762     456     393     385      66      45
            
 
BsrDI GCAATG_nn'

Cuts at:      0    3791    4716    5913    6135 
   Size:       3791     925    1197     222

  Fragments arranged by size: 

                3791    1197     925     222
 
BsrFI r'CCGG_y

Cuts at:      0       4       8      79    1196    1340    2060    2991    3695 
   Size:          4       4      71    1117     144     720     931     704

Cuts at:   3695    4122    4317    4341    4540    5923    6068    6135 
   Size:        427     195      24     199    1383     145      67

  Fragments arranged by size: 

                1383    1117     931     720     704     427     199     195
                 145     144      71      67      24       4       4
 
BsrGI T'GTAC_A

Cuts at:      0    2530    4229    6135 
   Size:       2530    1699    1906

  Fragments arranged by size: 

                2530    1906    1699
 
BssHII G'CGCG_C

Cuts at:      0      99     101     778    1585    6135 
   Size:         99       2     677     807    4550

  Fragments arranged by size: 

                4550     807     677      99       2
 
BssSI C'ACGA_G

Cuts at:      0     804    2598    5009    6135 
   Size:        804    1794    2411    1126

  Fragments arranged by size: 

                2411    1794    1126     804
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     659    1149    1535    1925    4868    5412    6078    6135 
   Size:        659     490     386     390    2943     544     666      57

  Fragments arranged by size: 

                2943     666     659     544     490     390     386      57
            
 
BstEII G'GTnAC_C

Cuts at:      0    2166    5443    6135 
   Size:       2166    3277     692

  Fragments arranged by size: 

                3277    2166     692
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     371     615    3156    5367    5952    6135 
   Size:        371     244    2541    2211     585     183

  Fragments arranged by size: 

                2541    2211     585     371     244     183
 
BstYI r'GATC_y

Cuts at:      0     158     338    1574    3677    6135 
   Size:        158     180    1236    2103    2458

  Fragments arranged by size: 

                2458    2103    1236     180     158
 
Bsu36I CC'TnA_GG

Cuts at:      0    2461    4214    4546    5463    6135 
   Size:       2461    1753     332     917     672

  Fragments arranged by size: 

                2461    1753     917     672     332
 
BtgI C'CryG_G

Cuts at:      0     714     828    1224    1550    2391    2663    3128    3806 
   Size:        714     114     396     326     841     272     465     678

Cuts at:   3806    3902    4017    4511    4674    4791    5735    5813    5999 
   Size:         96     115     494     163     117     944      78     186

Cuts at:   5999    6135 
   Size:        136

  Fragments arranged by size: 

                 944     841     714     678     494     465     396     326
                 272     186     163     136     117     115     114      96
                  78
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2841    3047    3215    3812    6135 
   Size:       2841     206     168     597    2323

  Fragments arranged by size: 

                2841    2323     597     206     168
 
BtsI GCAGTG_nn'

Cuts at:      0     685    1420    1621    1855    1926    2641    2986    3077 
   Size:        685     735     201     234      71     715     345      91

Cuts at:   3077    3821    3935    5080    5550    5659    6010    6135 
   Size:        744     114    1145     470     109     351     125

  Fragments arranged by size: 

                1145     744     735     715     685     470     351     345
                 234     201     125     114     109      91      71
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2790    2825    6135 
   Size:       2790      35    3310

  Fragments arranged by size: 

                3310    2790      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2183    3104    6135 
   Size:       2183     921    3031

  Fragments arranged by size: 

                3031    2183     921
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1081    6135 
   Size:       1081    5054
 
EaeI y'GGCC_r

Cuts at:      0       2       6      31      56     373     785    1158    1227 
   Size:          2       4      25      25     317     412     373      69

Cuts at:   1227    2517    2646    3278    3715    3809    4281    4734    5196 
   Size:       1290     129     632     437      94     472     453     462

Cuts at:   5196    5762    6135 
   Size:        566     373

  Fragments arranged by size: 

                1290     632     566     472     462     453     437     412
                 373     373     317     129      94      69      25      25
                   4       2
 
EagI C'GGCC_G

Cuts at:      0       6      31     785    2646    3715    4734    5196    6135 
   Size:          6      25     754    1861    1069    1019     462     939

  Fragments arranged by size: 

                1861    1069    1019     939     754     462      25       6
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     589    3532    4759    6135 
   Size:        589    2943    1227    1376

  Fragments arranged by size: 

                2943    1376    1227     589
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     691    3044    3153    5628    6135 
   Size:        691    2353     109    2475     507

  Fragments arranged by size: 

                2475    2353     691     507     109
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     390     457     470    1564    1936    2103    2212 
   Size:        127     263      67      13    1094     372     167     109

Cuts at:   2212    2869    3433    3439    3624    4228    4390    4540    4609 
   Size:        657     564       6     185     604     162     150      69

Cuts at:   4609    4720    4957    5011    5119    5206    5295    5296    5742 
   Size:        111     237      54     108      87      89       1     446

Cuts at:   5742    6109    6135 
   Size:        367      26

  Fragments arranged by size: 

                1094     657     604     564     446     372     367     263
                 237     185     167     162     150     127     111     109
                 108      89      87      69      67      54      26      13
                   6       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     439    1636    2980    5119    5172    6088    6135 
   Size:        439    1197    1344    2139      53     916      47

  Fragments arranged by size: 

                2139    1344    1197     916     439      53      47
 
EcoO109I rG'GnC_Cy

Cuts at:      0     473     735    1268    1374    1389    1594    2254    2457 
   Size:        473     262     533     106      15     205     660     203

Cuts at:   2457    2659    2871    3576    3583    3663    3861    4025    4682 
   Size:        202     212     705       7      80     198     164     657

Cuts at:   4682    4683    4901    4968    5014    5486    5913    5982    6135 
   Size:          1     218      67      46     472     427      69     153

  Fragments arranged by size: 

                 705     660     657     533     473     472     427     262
                 218     212     205     203     202     198     164     153
                 106      80      69      67      46      15       7       1
            
 
EcoRI G'AATT_C

Cuts at:      0    3534    4195    6135 
   Size:       3534     661    1940

  Fragments arranged by size: 

                3534    1940     661
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    5082    6135 
   Size:       5082    1053
 
FseI GG_CCGG'CC

Cuts at:      0       8    6135 
   Size:          8    6127
 
FspI TGC'GCA

Cuts at:      0    5692    6135 
   Size:       5692     443
 
HaeII r_GCGC'y

Cuts at:      0     542     622     696     836     869     968    1017    1063 
   Size:        542      80      74     140      33      99      49      46

Cuts at:   1063    1656    2561    3008    3696    4091    4115    4207    4273 
   Size:        593     905     447     688     395      24      92      66

Cuts at:   4273    4392    4519    4541    4587    4804    4990    5303    5945 
   Size:        119     127      22      46     217     186     313     642

Cuts at:   5945    6135 
   Size:        190

  Fragments arranged by size: 

                 905     688     642     593     542     447     395     313
                 217     190     186     140     127     119      99      92
                  80      74      66      49      46      46      33      24
                  22
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     727     759    2074    2106    2492    2524    3036    3068 
   Size:        727      32    1315      32     386      32     512      32

Cuts at:   3068    3457    3489    4076    4108    5056    5088    5416    5448 
   Size:        389      32     587      32     948      32     328      32

Cuts at:   5448    6135 
   Size:        687

  Fragments arranged by size: 

                1315     948     727     687     587     512     389     386
                 328      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    4575    5841    6135 
   Size:       4575    1266     294

  Fragments arranged by size: 

                4575    1266     294
 
KasI G'GCGC_C

Cuts at:      0     538     618     832     865     964    1013    1059    2557 
   Size:        538      80     214      33      99      49      46    1498

Cuts at:   2557    3004    3692    4087    4111    4388    4537    4800    5941 
   Size:        447     688     395      24     277     149     263    1141

Cuts at:   5941    6135 
   Size:        194

  Fragments arranged by size: 

                1498    1141     688     538     447     395     277     263
                 214     194     149      99      80      49      46      33
                  24
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     614     770    1730    3401    4722    6135 
   Size:        520      94     156     960    1671    1321    1413

  Fragments arranged by size: 

                1671    1413    1321     960     520     156      94
 
MscI TGG'CCA

Cuts at:      0     375    3280    5764    6135 
   Size:        375    2905    2484     371

  Fragments arranged by size: 

                2905    2484     375     371
 
MslI CAynn'nnrTG

Cuts at:      0     369     487    3394    6135 
   Size:        369     118    2907    2741

  Fragments arranged by size: 

                2907    2741     369     118
 
MspA1I CmG'CkG

Cuts at:      0      27      36     284     672     716     989    1238    1607 
   Size:         27       9     248     388      44     273     249     369

Cuts at:   1607    1786    2123    2271    2474    2543    2665    2847    3720 
   Size:        179     337     148     203      69     122     182     873

Cuts at:   3720    4067    5021    5131    5367    5391    5581    5852    5937 
   Size:        347     954     110     236      24     190     271      85

Cuts at:   5937    5952    6078    6135 
   Size:         15     126      57

  Fragments arranged by size: 

                 954     873     388     369     347     337     273     271
                 249     248     236     203     190     182     179     148
                 126     122     110      85      69      57      44      27
                  24      15       9
 
NaeI GCC'GGC

Cuts at:      0       6      81    1342    2062    2993    4124    4319    4542 
   Size:          6      75    1261     720     931    1131     195     223

Cuts at:   4542    6070    6135 
   Size:       1528      65

  Fragments arranged by size: 

                1528    1261    1131     931     720     223     195      75
                  65       6
 
NarI GG'CG_CC

Cuts at:      0     539     619     833     866     965    1014    1060    2558 
   Size:        539      80     214      33      99      49      46    1498

Cuts at:   2558    3005    3693    4088    4112    4389    4538    4801    5942 
   Size:        447     688     395      24     277     149     263    1141

Cuts at:   5942    6135 
   Size:        193

  Fragments arranged by size: 

                1498    1141     688     539     447     395     277     263
                 214     193     149      99      80      49      46      33
                  24
 
NcoI C'CATG_G

Cuts at:      0    2391    4674    4791    6135 
   Size:       2391    2283     117    1344

  Fragments arranged by size: 

                2391    2283    1344     117
 
NgoMIV G'CCGG_C

Cuts at:      0       4      79    1340    2060    2991    4122    4317    4540 
   Size:          4      75    1261     720     931    1131     195     223

Cuts at:   4540    6068    6135 
   Size:       1528      67

  Fragments arranged by size: 

                1528    1261    1131     931     720     223     195      75
                  67       4
 
NotI GC'GGCC_GC

Cuts at:      0      31    3715    6135 
   Size:         31    3684    2420

  Fragments arranged by size: 

                3684    2420      31
 
NruI TCG'CGA

Cuts at:      0    3066    6135 
   Size:       3066    3069
 
NspI r_CATG'y

Cuts at:      0      92    1737    6135 
   Size:         92    1645    4398

  Fragments arranged by size: 

                4398    1645      92
 
PasI CC'CwG_GG

Cuts at:      0    2238    2655    2965    3286    4848    5160    6135 
   Size:       2238     417     310     321    1562     312     975

  Fragments arranged by size: 

                2238    1562     975     417     321     312     310
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1069    2895    3104    4587    4698    5814    5852    6135 
   Size:       1069    1826     209    1483     111    1116      38     283

  Fragments arranged by size: 

                1826    1483    1116    1069     283     209     111      38
            
 
PfoI T'CCnGG_A

Cuts at:      0    3410    6135 
   Size:       3410    2725
 
PmlI CAC'GTG

Cuts at:      0    1743    1776    3732    5409    6135 
   Size:       1743      33    1956    1677     726

  Fragments arranged by size: 

                1956    1743    1677     726      33
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     433     465    4984    5016    5874    5906    6135 
   Size:        433      32    4519      32     858      32     229

  Fragments arranged by size: 

                4519     858     433     229      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     473     735    1594    3576    4025    4968    6135 
   Size:        473     262     859    1982     449     943    1167

  Fragments arranged by size: 

                1982    1167     943     859     473     449     262
 
PshAI GACnn'nnGTC

Cuts at:      0     956    5176    5620    6135 
   Size:        956    4220     444     515

  Fragments arranged by size: 

                4220     956     515     444
 
PspOMI G'GGCC_C

Cuts at:      0      21    1374    2658    2898    3583    3662    3860    4682 
   Size:         21    1353    1284     240     685      79     198     822

Cuts at:   4682    4900    6003    6135 
   Size:        218    1103     132

  Fragments arranged by size: 

                1353    1284    1103     822     685     240     218     198
                 132      79      21
 
PspXI vC'TCGA_Gb

Cuts at:      0    1482    3253    6135 
   Size:       1482    1771    2882

  Fragments arranged by size: 

                2882    1771    1482
 
PstI C_TGCA'G

Cuts at:      0     671     760    1145    1475    2542    3725    4066    5666 
   Size:        671      89     385     330    1067    1183     341    1600

Cuts at:   5666    5900    6135 
   Size:        234     235

  Fragments arranged by size: 

                1600    1183    1067     671     385     341     330     235
                 234      89
 
PvuI CG_AT'CG

Cuts at:      0    1117    6135 
   Size:       1117    5018
 
PvuII CAG'CTG

Cuts at:      0     989    1786    2123    2543    2847    4067    5367    5391 
   Size:        989     797     337     420     304    1220    1300      24

Cuts at:   5391    5952    6135 
   Size:        561     183

  Fragments arranged by size: 

                1300    1220     989     797     561     420     337     304
                 183      24
 
SacII CC_GC'GG

Cuts at:      0     717    2666    6135 
   Size:        717    1949    3469

  Fragments arranged by size: 

                3469    1949     717
 
SanDI GG'GwC_CC

Cuts at:      0     473     735    6135 
   Size:        473     262    5400

  Fragments arranged by size: 

                5400     473     262
 
SapI GCTCTTCn'nnn_

Cuts at:      0     589    4759    6135 
   Size:        589    4170    1376

  Fragments arranged by size: 

                4170    1376     589
 
SbfI CC_TGCA'GG

Cuts at:      0    1475    5900    6135 
   Size:       1475    4425     235

  Fragments arranged by size: 

                4425    1475     235
 
SexAI A'CCwGG_T

Cuts at:      0     233     314    6135 
   Size:        233      81    5821

  Fragments arranged by size: 

                5821     233      81
 
SfcI C'TryA_G

Cuts at:      0     648     667     756    1141    1272    1471    2172    2538 
   Size:        648      19      89     385     131     199     701     366

Cuts at:   2538    3721    4062    5662    5896    6135 
   Size:       1183     341    1600     234     239

  Fragments arranged by size: 

                1600    1183     701     648     385     366     341     239
                 234     199     131      89      19
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    5021    6135 
   Size:       5021    1114
 
SfoI GGC'GCC

Cuts at:      0     540     620     834     867     966    1015    1061    2559 
   Size:        540      80     214      33      99      49      46    1498

Cuts at:   2559    3006    3694    4089    4113    4390    4539    4802    5943 
   Size:        447     688     395      24     277     149     263    1141

Cuts at:   5943    6135 
   Size:        192

  Fragments arranged by size: 

                1498    1141     688     540     447     395     277     263
                 214     192     149      99      80      49      46      33
                  24
 
SmaI CCC'GGG

Cuts at:      0      20     244     471    1129    1978    2344    2610    3661 
   Size:         20     224     227     658     849     366     266    1051

Cuts at:   3661    4246    5458    5532    6135 
   Size:        585    1212      74     603

  Fragments arranged by size: 

                1212    1051     849     658     603     585     366     266
                 227     224      74      20
 
SmlI C'TyrA_G

Cuts at:      0    1482    3253    6135 
   Size:       1482    1771    2882

  Fragments arranged by size: 

                2882    1771    1482
 
SnaBI TAC'GTA

Cuts at:      0    2229    5292    6135 
   Size:       2229    3063     843

  Fragments arranged by size: 

                3063    2229     843
 
SphI G_CATG'C

Cuts at:      0    1737    6135 
   Size:       1737    4398
 
SrfI GCCC'GGGC

Cuts at:      0    1978    3661    6135 
   Size:       1978    1683    2474

  Fragments arranged by size: 

                2474    1978    1683
 
StuI AGG'CCT

Cuts at:      0    4949    6022    6135 
   Size:       4949    1073     113

  Fragments arranged by size: 

                4949    1073     113
 
StyI C'CwwG_G

Cuts at:      0    2391    2553    2801    3260    3392    3701    4674    4791 
   Size:       2391     162     248     459     132     309     973     117

Cuts at:   4791    5597    6017    6135 
   Size:        806     420     118

  Fragments arranged by size: 

                2391     973     806     459     420     309     248     162
                 132     118     117
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3162    5659    6135 
   Size:       3162    2497     476

  Fragments arranged by size: 

                3162    2497     476
 
TatI w'GTAC_w

Cuts at:      0    2530    4229    6135 
   Size:       2530    1699    1906

  Fragments arranged by size: 

                2530    1906    1699
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     175     207    1799    1831    2089    2121    4181    4213 
   Size:        175      32    1592      32     258      32    2060      32

Cuts at:   4213    6135 
   Size:       1922

  Fragments arranged by size: 

                2060    1922    1592     258     175      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1081    1102    1252    1516    1906    6097    6135 
   Size:       1081      21     150     264     390    4191      38

  Fragments arranged by size: 

                4191    1081     390     264     150      38      21
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     322    1696    3229    5181    5709    6135 
   Size:        322    1374    1533    1952     528     426

  Fragments arranged by size: 

                1952    1533    1374     528     426     322
 
XhoI C'TCGA_G

Cuts at:      0    1482    3253    6135 
   Size:       1482    1771    2882

  Fragments arranged by size: 

                2882    1771    1482
 
XmaI C'CCGG_G

Cuts at:      0      18     242     469    1127    1976    2342    2608    3659 
   Size:         18     224     227     658     849     366     266    1051

Cuts at:   3659    4244    5456    5530    6135 
   Size:        585    1212      74     605

  Fragments arranged by size: 

                1212    1051     849     658     605     585     366     266
                 227     224      74      18
 
XmnI GAAnn'nnTTC

Cuts at:      0     925    3553    6135 
   Size:        925    2628    2582

  Fragments arranged by size: 

                2628    2582     925
 
ZraI GAC'GTC

Cuts at:      0    3543    3961    4572    5427    6135 
   Size:       3543     418     611     855     708

  Fragments arranged by size: 

                3543     855     708     611     418
 

 Enzymes that do cut:

     AarI    AatII     AcuI   AflIII     AhdI     AleI     AlfI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    HaeII
    Hin4I   HincII     KasI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NotI     NruI     NspI     PasI    PflMI
     PfoI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI
     PvuI    PvuII    SacII    SanDI     SapI     SbfI    SexAI     SfcI
     SfiI     SfoI     SmaI     SmlI    SnaBI     SphI     SrfI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BfrBI    BglII     BmtI    BpuEI
    BsaBI    BsiWI    BsmBI    BspHI    BstBI  BstZ17I     ClaI     DraI
  EcoICRI    EcoRV    FspAI  HindIII     HpaI     KpnI     MfeI     MluI
     NdeI     NheI     NsiI     PacI     PciI     PmeI     PsiI     PsrI
    RsrII     SacI     SalI     ScaI     SgfI    SgrAI     SpeI     SspI
     SwaI     XbaI
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