Restriction map for pF1KB9482
 (Linear) (Six-base) MAPSORT of: KIBB9482.seq  Check: 7234  from: 1  to: 1551



KIBB9482.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1347    1551 
   Size:       1347     204
 
AccI GT'mk_AC

Cuts at:      0    1306    1551 
   Size:       1306     245
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     504    1551 
   Size:        504    1047
 
AflIII A'CryG_T

Cuts at:      0     362     818    1472    1551 
   Size:        362     456     654      79

  Fragments arranged by size: 

                 654     456     362      79
 
AhdI GACnn_n'nnGTC

Cuts at:      0     819    1551 
   Size:        819     732
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1293    1551 
   Size:       1293     258
 
ApaI G_GGCC'C

Cuts at:      0     893    1551 
   Size:        893     658
 
ApaLI G'TGCA_C

Cuts at:      0     418    1551 
   Size:        418    1133
 
AvaI C'yCGr_G

Cuts at:      0     277    1222    1504    1551 
   Size:        277     945     282      47

  Fragments arranged by size: 

                 945     282     277      47
 
AvrII C'CTAG_G

Cuts at:      0       9    1551 
   Size:          9    1542
 
BanI G'GyrC_C

Cuts at:      0     711     870     984    1551 
   Size:        711     159     114     567

  Fragments arranged by size: 

                 711     567     159     114
 
BanII G_rGCy'C

Cuts at:      0     893    1141    1551 
   Size:        893     248     410

  Fragments arranged by size: 

                 893     410     248
 
BbeI G_GCGC'C

Cuts at:      0     715     874    1551 
   Size:        715     159     677

  Fragments arranged by size: 

                 715     677     159
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1105    1447    1551 
   Size:       1105     342     104

  Fragments arranged by size: 

                1105     342     104
 
BbvCI CC'TCA_GC

Cuts at:      0    1074    1551 
   Size:       1074     477
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     594     628    1234    1268    1551 
   Size:        594      34     606      34     283

  Fragments arranged by size: 

                 606     594     283      34      34
 
BclI T'GATC_A

Cuts at:      0    1517    1551 
   Size:       1517      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     406     475    1160    1551 
   Size:        406      69     685     391

  Fragments arranged by size: 

                 685     406     391      69
 
BlpI GC'TnA_GC

Cuts at:      0    1450    1551 
   Size:       1450     101
 
Bme1580I G_kGCm'C

Cuts at:      0     391     422     893    1551 
   Size:        391      31     471     658

  Fragments arranged by size: 

                 658     471     391      31
 
BmgBI CAC'GTC

Cuts at:      0     365    1353    1551 
   Size:        365     988     198

  Fragments arranged by size: 

                 988     365     198
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     378    1551 
   Size:        378    1173
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1089    1551 
   Size:       1089     462
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     649     661     816    1551 
   Size:        160     489      12     155     735

  Fragments arranged by size: 

                 735     489     160     155      12
 
Bpu10I CC'TnA_GC

Cuts at:      0    1074    1551 
   Size:       1074     477
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     171     247     529    1125    1474    1528    1551 
   Size:        171      76     282     596     349      54      23

  Fragments arranged by size: 

                 596     349     282     171      76      54      23
 
BsaAI yAC'GTr

Cuts at:      0     122    1551 
   Size:        122    1429
 
BsaHI Gr'CG_yC

Cuts at:      0     712     871    1102    1551 
   Size:        712     159     231     449

  Fragments arranged by size: 

                 712     449     231     159
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     634     664    1551 
   Size:        634      30     887

  Fragments arranged by size: 

                 887     634      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     190     193     244     487     496     499     510     586 
   Size:        190       3      51     243       9       3      11      76

Cuts at:    586     589     691     898     940     949     952     967     979 
   Size:          3     102     207      42       9       3      15      12

Cuts at:    979     982     985     988    1042    1060    1062    1182    1551 
   Size:          3       3       3      54      18       2     120     369

  Fragments arranged by size: 

                 369     243     207     190     120     102      76      54
                  51      42      18      15      12      11       9       9
                   3       3       3       3       3       3       3       2
            
 
BseYI C'CCAG_C

Cuts at:      0    1168    1313    1551 
   Size:       1168     145     238

  Fragments arranged by size: 

                1168     238     145
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1375    1551 
   Size:       1375     176
 
BsiHKAI G_wGCw'C

Cuts at:      0     422    1141    1551 
   Size:        422     719     410

  Fragments arranged by size: 

                 719     422     410
 
Bsp1286I G_dGCh'C

Cuts at:      0     391     422     893    1141    1551 
   Size:        391      31     471     248     410

  Fragments arranged by size: 

                 471     410     391     248      31
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1347    1424    1551 
   Size:       1347      77     127

  Fragments arranged by size: 

                1347     127      77
 
BsrBI CCG'CTC

Cuts at:      0     144    1119    1551 
   Size:        144     975     432

  Fragments arranged by size: 

                 975     432     144
 
BstYI r'GATC_y

Cuts at:      0    1484    1551 
   Size:       1484      67
 
Bsu36I CC'TnA_GG

Cuts at:      0     950    1551 
   Size:        950     601
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     439     797    1551 
   Size:        439     358     754

  Fragments arranged by size: 

                 754     439     358
 
BtsI GCAGTG_nn'

Cuts at:      0     210     464    1551 
   Size:        210     254    1087

  Fragments arranged by size: 

                1087     254     210
 
DraIII CAC_nnn'GTG

Cuts at:      0     386    1551 
   Size:        386    1165
 
EaeI y'GGCC_r

Cuts at:      0     591     719    1551 
   Size:        591     128     832

  Fragments arranged by size: 

                 832     591     128
 
EarI CTCTTCn'nnn_

Cuts at:      0     842    1087    1551 
   Size:        842     245     464

  Fragments arranged by size: 

                 842     464     245
 
EcoICRI GAG'CTC

Cuts at:      0    1139    1551 
   Size:       1139     412
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     504     649     661     816    1551 
   Size:        160     344     145      12     155     735

  Fragments arranged by size: 

                 735     344     160     155     145      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0      13     398     889    1282    1413    1551 
   Size:         13     385     491     393     131     138

  Fragments arranged by size: 

                 491     393     385     138     131      13
 
FspI TGC'GCA

Cuts at:      0     616    1551 
   Size:        616     935
 
HaeII r_GCGC'y

Cuts at:      0     715     874    1551 
   Size:        715     159     677

  Fragments arranged by size: 

                 715     677     159
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     349     381     634     666     805     837    1551 
   Size:        349      32     253      32     139      32     714

  Fragments arranged by size: 

                 714     349     253     139      32      32      32
 
HincII GTy'rAC

Cuts at:      0     111     306    1307    1551 
   Size:        111     195    1001     244

  Fragments arranged by size: 

                1001     244     195     111
 
HindIII A'AGCT_T

Cuts at:      0     220    1551 
   Size:        220    1331
 
KasI G'GCGC_C

Cuts at:      0     711     870    1551 
   Size:        711     159     681

  Fragments arranged by size: 

                 711     681     159
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1013    1551 
   Size:       1013     538
 
MscI TGG'CCA

Cuts at:      0     721    1551 
   Size:        721     830
 
MspA1I CmG'CkG

Cuts at:      0     444     537     717    1346    1551 
   Size:        444      93     180     629     205

  Fragments arranged by size: 

                 629     444     205     180      93
 
NarI GG'CG_CC

Cuts at:      0     712     871    1551 
   Size:        712     159     680

  Fragments arranged by size: 

                 712     680     159
 
NspI r_CATG'y

Cuts at:      0     822    1551 
   Size:        822     729
 
PciI A'CATG_T

Cuts at:      0     818    1551 
   Size:        818     733
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     627     810    1551 
   Size:        627     183     741

  Fragments arranged by size: 

                 741     627     183
 
PpuMI rG'GwC_Cy

Cuts at:      0    1282    1413    1551 
   Size:       1282     131     138

  Fragments arranged by size: 

                1282     138     131
 
PspOMI G'GGCC_C

Cuts at:      0     889    1551 
   Size:        889     662
 
PspXI vC'TCGA_Gb

Cuts at:      0    1222    1551 
   Size:       1222     329
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     352     384    1551 
   Size:        352      32    1167

  Fragments arranged by size: 

                1167     352      32
 
PstI C_TGCA'G

Cuts at:      0    1257    1551 
   Size:       1257     294
 
SacI G_AGCT'C

Cuts at:      0    1141    1551 
   Size:       1141     410
 
SalI G'TCGA_C

Cuts at:      0    1305    1551 
   Size:       1305     246
 
SanDI GG'GwC_CC

Cuts at:      0    1282    1551 
   Size:       1282     269
 
SapI GCTCTTCn'nnn_

Cuts at:      0     842    1551 
   Size:        842     709
 
SfcI C'TryA_G

Cuts at:      0    1253    1551 
   Size:       1253     298
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     406    1551 
   Size:        406    1145
 
SfoI GGC'GCC

Cuts at:      0     713     872    1551 
   Size:        713     159     679

  Fragments arranged by size: 

                 713     679     159
 
SmlI C'TyrA_G

Cuts at:      0     150     262     544    1140    1222    1489    1543    1551 
   Size:        150     112     282     596      82     267      54       8

  Fragments arranged by size: 

                 596     282     267     150     112      82      54       8
            
 
StuI AGG'CCT

Cuts at:      0     409     769    1069    1551 
   Size:        409     360     300     482

  Fragments arranged by size: 

                 482     409     360     300
 
StyI C'CwwG_G

Cuts at:      0       9     695     722     866     893    1551 
   Size:          9     686      27     144      27     658

  Fragments arranged by size: 

                 686     658     144      27      27       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     397    1002    1122    1353    1551 
   Size:        397     605     120     231     198

  Fragments arranged by size: 

                 605     397     231     198     120
 
Tth111I GACn'n_nGTC

Cuts at:      0     106    1551 
   Size:        106    1445
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1294    1551 
   Size:       1294     257
 
XhoI C'TCGA_G

Cuts at:      0    1222    1551 
   Size:       1222     329
 
XmnI GAAnn'nnTTC

Cuts at:      0     517    1426    1551 
   Size:        517     909     125

  Fragments arranged by size: 

                 909     517     125
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AhdI    AlwNI     ApaI    ApaLI
     AvaI    AvrII     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
     BclI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrBI    BstYI   Bsu36I    BtgZI     BtsI
   DraIII     EaeI     EarI  EcoICRI  Eco57MI EcoO109I     FspI    HaeII
    Hin4I   HincII  HindIII     KasI     MmeI     MscI   MspA1I     NarI
     NspI     PciI    PflMI    PpuMI   PspOMI    PspXI     PsrI     PstI
     SacI     SalI    SanDI     SapI     SfcI     SfiI     SfoI     SmlI
     StuI     StyI    TaqII  Tth111I     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI     AlfI
     AloI     ApoI     AscI     AseI    AsiSI     BaeI    BamHI    BciVI
    BfrBI    BglII     BmtI     BsaI    BsaBI    BsaWI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI     ClaI
    CspCI     DraI     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV
     FalI     FseI    FspAI     HpaI     KpnI     MfeI     MluI     MslI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
     PvuI    PvuII    RsrII    SacII     SbfI     ScaI    SexAI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI     TstI     XbaI     XmaI     ZraI
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