Restriction map for pF1KB9479
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AviII       AvrII       
BamHI       BbeI        BbrPI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BsaAI       
BsaBI       BsaHI       BseAI       BsiEI       BsiWI       
BspDI       BspEI       BsrDI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CfoI        ClaI        CpoI        DraI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRV       EcoT22I     FbaI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        MamI        MfeI        MluI        MroI        
MunI        NarI        NdeI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RsrII       SacI        SalI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
SspI        SwaI        TaqI        TthHB8I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccII        1261  CGCG                 CG^CG
AcsI         1401  GAATTC               [GA]^AATT[CT]
AflIII        587  ACATGT               A^C[GA][CT]GT
ApoI         1401  GAATTC               [GA]^AATT[CT]
Asp700I       880  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1462  GAGCAC               G[AT]GC[AT]^C
AvaI          706  CCCGAG               C^[CT]CG[GA]G
BalI          824  TGGCCA               TGG^CCA
BbsI          806  GTCTTC               GAAGAC(2/6)
BpuAI         806  GTCTTC               GAAGAC(2/6)
BsaWI        1451  ACCGGA               [AT]^CCGG[AT]
BsgI          557  GTGCAG               GTGCAG(16/14)
BsiHKAI      1462  GAGCAC               G[AT]GC[AT]^C
BsmBI         480  GAGACG               CGTCTC(1/5)
BsoBI         706  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     1221  TGTACA               T^GTACA
BspHI        1950  TCATGA               T^CATGA
BspLU11I      587  ACATGT               A^CATGT
BsrBI         685  GAGCGG               CCGCTC(-3/-3)
BsrFI         499  GCCGGC               [GA]^CCGG[CT]
BsrGI        1221  TGTACA               T^GTACA
BstAPI        993  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1261  CGCG                 CG^CG
BstYI        1230  GGATCT               [GA]^GATC[CT]
Cfr10I        499  GCCGGC               [GA]^CCGG[CT]
DraIII       1079  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         280  CCTGGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1401  GAATTC               G^AATTC
FseI          498  GGCCGGCC             GGCCGG^CC
HgaI          180  GACGC                GACGC(5/10)
KspI         1260  CCGCGG               CCGC^GG
MaeII         458  ACGT                 A^CGT
MflI         1230  GGATCT               [GA]^GATC[CT]
MluNI         824  TGGCCA               TGG^CCA
MscI          824  TGGCCA               TGG^CCA
MseI         1180  TTAA                 T^TAA
MvnI         1261  CGCG                 CG^CG
NaeI          499  GCCGGC               GCC^GGC
NgoMIV        499  GCCGGC               G^CCGGC
NheI         1818  GCTAGC               G^CTAGC
NspI          587  ACATGT               [GA]CATG^[CT]
PshAI         231  GACTGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1950  TCATGA               T^CATGA
SacII        1260  CCGCGG               CCGC^GG
SspBI        1221  TGTACA               T^GTACA
Tru9I        1180  TTAA                 T^TAA
XhoII        1230  GGATCT               [GA]^GATC[CT]
XmnI          880  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AfaI         1222  GTAC                 GT^AC
             1743  GTAC                
AlwI          413  GGATC                GGATC(4/5)
             1230  GGATC               
AspI          778  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1030  GACTGTGTC           
BbvCI         508  CCTCAGC              CCTCAGC(-5/-2)
             1165  GCTGAGG             
BlpI         1288  GCTGAGC              GC^T[ACGT]AGC
             1459  GCTGAGC             
Bpu1102I     1288  GCTGAGC              GC^T[ACGT]AGC
             1459  GCTGAGC             
BsmI          927  GAATGC               GAATGC(1/-1)
              215  GCATTC              
CelII        1288  GCTGAGC              GC^T[ACGT]AGC
             1459  GCTGAGC             
DpnII         414  GATC                 ^GATC
             1231  GATC                
DrdI          292  GACACAGTAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1345  GACCCTGGGGTC        
EaeI          824  TGGCCA               [CT]^GGCC[GA]
             1491  TGGCCG              
MboI          414  GATC                 ^GATC
             1231  GATC                
MslI          324  CACCGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              453  CACCGACGTG          
NdeII         414  GATC                 ^GATC
             1231  GATC                
PflFI         778  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1030  GACTGTGTC           
PflMI        1014  CCAGGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1299  CCACAGATTGG         
RsaI         1222  GTAC                 GT^AC
             1743  GTAC                
SapI          880  GAAGAGC              GCTCTTC(1/4)
              183  GCTCTTC             
Sau3AI        414  GATC                 ^GATC
             1231  GATC                
SbfI          111  CCTGCAGG             CCTGCA^GG
              312  CCTGCAGG            
Sse8387I      111  CCTGCAGG             CCTGCA^GG
              312  CCTGCAGG            
StuI          336  AGGCCT               AGG^CCT
             1373  AGGCCT              
TfiI           97  GAATC                G^A[AT]TC
             1486  GAATC               
Tsp509I       152  AATT                 ^AATT
             1402  AATT                
Tth111I       778  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1030  GACTGTGTC           
Van91I       1014  CCAGGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1299  CCACAGATTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AluI        AlwNI       ApaI        AvaII       
BanI        BanII       BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BsaI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstXI       Cac8I       Cfr13I      DdeI        
DraII       DsaI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboII       MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NcoI        NlaIII      NlaIV       PleI        PpuMI       
PstI        PvuII       Sau96I      ScrFI       SfaNI       
SfcI        StyI        TseI        Tsp45I      TspRI       
XcmI        
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