Restriction map for pF1KB9464
 (Circular) (Six-base) MAPSORT of: pF1KB9464.seq  Check: 8559  from: 1  to: 6683



pF1KB9464  6683 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:30  ..

AatII G_ACGT'C

Cuts at:   1368    1368 
   Size:       6683
 
AccI GT'mk_AC

Cuts at:   2009    3721    2009 
   Size:       1712    4971
 
Acc65I G'GTAC_C

Cuts at:   3699    3699 
   Size:       6683
 
AclI AA'CG_TT

Cuts at:   6523    6523 
   Size:       6683
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    472    1432    1628    1795    2786    3277    4508    4940    5293 
   Size:        960     196     167     991     491    1231     432     353

Cuts at:   5293    5955     472 
   Size:        662    1200

  Fragments arranged by size: 

                1231    1200     991     960     662     491     432     353
                 196     167
 
AflIII A'CryG_T

Cuts at:   1187    2653    4035    5835    6116    1187 
   Size:       1466    1382    1800     281    1754

  Fragments arranged by size: 

                1800    1754    1466    1382     281
 
AhdI GACnn_n'nnGTC

Cuts at:   2305    2566    2808    2305 
   Size:        261     242    6180

  Fragments arranged by size: 

                6180     261     242
 
AleI CACnn'nnGTG

Cuts at:   2422    2422 
   Size:       6683
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   3341    3341 
   Size:       6683
 
AlwNI CAG_nnn'CTG

Cuts at:   1773    1974    2407    3493    3615    3988    5426    1773 
   Size:        201     433    1086     122     373    1438    3030

  Fragments arranged by size: 

                3030    1438    1086     433     373     201     122
 
ApaI G_GGCC'C

Cuts at:    660     927    1022     660 
   Size:        267      95    6321

  Fragments arranged by size: 

                6321     267      95
 
ApaLI G'TGCA_C

Cuts at:   2657    5521    2657 
   Size:       2864    3819
 
ApoI r'AATT_y

Cuts at:   2688    3687    6036    6292    2688 
   Size:        999    2349     256    3079

  Fragments arranged by size: 

                3079    2349     999     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6683
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6683
 
AvaI C'yCGr_G

Cuts at:    318     743     803     944     997    1581    3703     318 
   Size:        425      60     141      53     584    2122    3298

  Fragments arranged by size: 

                3298    2122     584     425     141      60      53
 
AvrII C'CTAG_G

Cuts at:   1003    1003 
   Size:       6683
 
BamHI G'GATC_C

Cuts at:   3708    3708 
   Size:       6683
 
BanI G'GyrC_C

Cuts at:    376    2896    3699    4113    4361    4396     376 
   Size:       2520     803     414     248      35    2663

  Fragments arranged by size: 

                2663    2520     803     414     248      35
 
BanII G_rGCy'C

Cuts at:    660     927     991    1022    3021    3407    3697    4727     660 
   Size:        267      64      31    1999     386     290    1030    2616

  Fragments arranged by size: 

                2616    1999    1030     386     290     267      64      31
            
 
BbeI G_GCGC'C

Cuts at:   2900    4117    4365    2900 
   Size:       1217     248    5218

  Fragments arranged by size: 

                5218    1217     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1989    2225    2807    2882    1989 
   Size:        236     582      75    5790

  Fragments arranged by size: 

                5790     582     236      75
 
BbvCI CC'TCA_GC

Cuts at:    679     679 
   Size:       6683
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124    1680    1714      90 
   Size:         34    1556      34    5059

  Fragments arranged by size: 

                5059    1556      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3555    4581    5637    6683    3555 
   Size:       1026    1056    1046    3555

  Fragments arranged by size: 

                3555    1056    1046    1026
 
BclI T'GATC_A

Cuts at:   2628    2790    4203    2628 
   Size:        162    1413    5108

  Fragments arranged by size: 

                5108    1413     162
 
BglI GCCn_nnn'nGGC

Cuts at:   1008    1008 
   Size:       6683
 
BglII A'GATC_T

Cuts at:   1085    4198    6235    1085 
   Size:       3113    2037    1533

  Fragments arranged by size: 

                3113    2037    1533
 
BlpI GC'TnA_GC

Cuts at:   3795    3795 
   Size:       6683
 
Bme1580I G_kGCm'C

Cuts at:    660     927    1022    2661    4308    4401    5525     660 
   Size:        267      95    1639    1647      93    1124    1818

  Fragments arranged by size: 

                1818    1647    1639    1124     267      95      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   2514    3895    3976    4311    6455    2514 
   Size:       1381      81     335    2144    2742

  Fragments arranged by size: 

                2742    2144    1381     335      81
 
BmtI G_CTAG'C

Cuts at:   3041    3041 
   Size:       6683
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5050    5050 
   Size:       6683
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    730     790    1337    2184    2441    2541    3391     730 
   Size:         60     547     847     257     100     850    4022

  Fragments arranged by size: 

                4022     850     847     547     257     100      60
 
Bpu10I CC'TnA_GC

Cuts at:    679    3527     679 
   Size:       2848    3835
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1144    1383    3266    3859    4738    5452    5750    1144 
   Size:        239    1883     593     879     714     298    2077

  Fragments arranged by size: 

                2077    1883     879     714     593     298     239
 
BsaI GGTCTCn'nnnn_

Cuts at:    116     740    1072    1516    1757    2292    2766    6387     116 
   Size:        624     332     444     241     535     474    3621     412

  Fragments arranged by size: 

                3621     624     535     474     444     412     332     241
            
 
BsaAI yAC'GTr

Cuts at:   4036    4036 
   Size:       6683
 
BsaBI GATnn'nnATC

Cuts at:      6    4197    6234       6 
   Size:       4191    2037     455

  Fragments arranged by size: 

                4191    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    642    1365    2897    4114    4362    5064    6560     642 
   Size:        723    1532    1217     248     702    1496     765

  Fragments arranged by size: 

                1532    1496    1217     765     723     702     248
 
BsaWI w'CCGG_w

Cuts at:    253     560     689    1676    2429    3872    4393    5071    5482 
   Size:        307     129     987     753    1443     521     678     411

Cuts at:   5482    5629    5903     253 
   Size:        147     274    1033

  Fragments arranged by size: 

                1443    1033     987     753     678     521     411     307
                 274     147     129
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1461    1491    1652    1682    2157    2187    2539    2569    2738 
   Size:         30     161      30     475      30     352      30     169

Cuts at:   2738    2768    3586    3616    1461 
   Size:         30     818      30    4528

  Fragments arranged by size: 

                4528     818     475     352     169     161      30      30
                  30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    134     594    1372    1553    2947    2952    2998    3042    3206 
   Size:        460     778     181    1394       5      46      44     164

Cuts at:   3206    3269    3307    3328    3607    3610    3613     134 
   Size:         63      38      21     279       3       3    3204

  Fragments arranged by size: 

                3204    1394     778     460     279     181     164      63
                  46      44      38      21       5       3       3
 
BseYI C'CCAG_C

Cuts at:    617     991    1557    2031    2143    2256    3001    3072    3503 
   Size:        374     566     474     112     113     745      71     431

Cuts at:   3503    4108    4866    5531    5939     617 
   Size:        605     758     665     408    1361

  Fragments arranged by size: 

                1361     758     745     665     605     566     474     431
                 408     374     113     112      71
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   2995    2995 
   Size:       6683
 
BsiEI CG_ry'CG

Cuts at:    104     834    1015    4225    4271    5501     104 
   Size:        730     181    3210      46    1230    1286

  Fragments arranged by size: 

                3210    1286    1230     730     181      46
 
BsiHKAI G_wGCw'C

Cuts at:    317     974    2661    3021    3407    3697    4475    4665    5525 
   Size:        657    1687     360     386     290     778     190     860

Cuts at:   5525     317 
   Size:       1475

  Fragments arranged by size: 

                1687    1475     860     778     657     386     360     290
                 190
 
BsmI GAATG_Cn'

Cuts at:   1718    2174    3076    3401    3426    1718 
   Size:        456     902     325      25    4975

  Fragments arranged by size: 

                4975     902     456     325      25
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1356    1356 
   Size:       6683
 
Bsp1286I G_dGCh'C

Cuts at:    317     660     927     974     991    1022    2661    3021    3407 
   Size:        343     267      47      17      31    1639     360     386

Cuts at:   3407    3697    4308    4401    4475    4665    4727    5525     317 
   Size:        290     611      93      74     190      62     798    1475

  Fragments arranged by size: 

                1639    1475     798     611     386     360     343     290
                 267     190      93      74      62      47      31      17
            
 
BspEI T'CCGG_A

Cuts at:    253     560     253 
   Size:        307    6376
 
BspHI T'CATG_A

Cuts at:   5115    5115 
   Size:       6683
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   3733    3947    4249    4630    3733 
   Size:        214     302     381    5786

  Fragments arranged by size: 

                5786     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     654     672     714     774     894     941    2960    4975 
   Size:        604      18      42      60     120      47    2019    2015

Cuts at:   4975    5029    6513    6678      50 
   Size:         54    1484     165      55

  Fragments arranged by size: 

                2019    2015    1484     604     165     120      60      55
                  54      47      42      18
 
BsrDI GCAATG_nn'

Cuts at:   1698    2342    4595    1698 
   Size:        644    2253    3786

  Fragments arranged by size: 

                3786    2253     644
 
BsrFI r'CCGG_y

Cuts at:    111     584    2970    4681    4862     111 
   Size:        473    2386    1711     181    1932

  Fragments arranged by size: 

                2386    1932    1711     473     181
 
BssHII G'CGCG_C

Cuts at:    736     736 
   Size:       6683
 
BssSI C'ACGA_G

Cuts at:   2980    3014    3510    4954    5662    2980 
   Size:         34     496    1444     708    4001

  Fragments arranged by size: 

                4001    1444     708     496      34
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3421    4060    3421 
   Size:        639    6044
 
BstBI TT'CG_AA

Cuts at:   5044    5044 
   Size:       6683
 
BstEII G'GTnAC_C

Cuts at:   1857    1857 
   Size:       6683
 
BstXI CCAn_nnnn'nTGG

Cuts at:    793    2185    2424     793 
   Size:       1392     239    5052

  Fragments arranged by size: 

                5052    1392     239
 
BstYI r'GATC_y

Cuts at:      1    1085    2404    3708    4175    4198    4533    4779    5085 
   Size:       1084    1319    1304     467      23     335     246     306

Cuts at:   5085    5097    5183    6235       1 
   Size:         12      86    1052     449

  Fragments arranged by size: 

                1319    1304    1084    1052     467     449     335     306
                 246      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   2203    2203 
   Size:       6683
 
BtgI C'CryG_G

Cuts at:    126     383     597     696     756     828    2825     126 
   Size:        257     214      99      60      72    1997    3984

  Fragments arranged by size: 

                3984    1997     257     214      99      72      60
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4633    4814    4977    4633 
   Size:        181     163    6339

  Fragments arranged by size: 

                6339     181     163
 
BtsI GCAGTG_nn'

Cuts at:    254    3315    3588    3926     254 
   Size:       3061     273     338    3011

  Fragments arranged by size: 

                3061    3011     338     273
 
DraI TTT'AAA

Cuts at:   3682    3682 
   Size:       6683
 
DraIII CAC_nnn'GTG

Cuts at:   2522    2522 
   Size:       6683
 
DrdI GACnn_nn'nnGTC

Cuts at:    361    4389    5733     361 
   Size:       4028    1344    1311

  Fragments arranged by size: 

                4028    1344    1311
 
EaeI y'GGCC_r

Cuts at:    479     699     759     831    1012    2175    3645    4268    4442 
   Size:        220      60      72     181    1163    1470     623     174

Cuts at:   4442    4833    4860    6205     479 
   Size:        391      27    1345     957

  Fragments arranged by size: 

                1470    1345    1163     957     623     391     220     181
                 174      72      60      27
 
EagI C'GGCC_G

Cuts at:    831    1012    4268     831 
   Size:        181    3256    3246

  Fragments arranged by size: 

                3256    3246     181
 
EarI CTCTTCn'nnn_

Cuts at:    176    1052    4706    4916     176 
   Size:        876    3654     210    1943

  Fragments arranged by size: 

                3654    1943     876     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1753    4891    5633    5779    6493    1753 
   Size:       3138     742     146     714    1943

  Fragments arranged by size: 

                3138    1943     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   3019    3405    3695    3019 
   Size:        386     290    6007

  Fragments arranged by size: 

                6007     386     290
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    472     730     790    1337    1432    1628    1795    2184    2441 
   Size:        258      60     547      95     196     167     389     257

Cuts at:   2441    2541    2786    3277    3391    4508    4940    5293    5955 
   Size:        100     245     491     114    1117     432     353     662

Cuts at:   5955     472 
   Size:       1200

  Fragments arranged by size: 

                1200    1117     662     547     491     432     389     353
                 258     257     245     196     167     114     100      95
                  60
 
EcoNI CCTnn'n_nnAGG

Cuts at:    217     508     217 
   Size:        291    6392
 
EcoO109I rG'GnC_Cy

Cuts at:    461     675     717     777     923     949    1018    1029    1730 
   Size:        214      42      60     146      26      69      11     701

Cuts at:   1730    1749    3822     461 
   Size:         19    2073    3322

  Fragments arranged by size: 

                3322    2073     701     214     146      69      60      42
                  26      19      11
 
EcoRI G'AATT_C

Cuts at:   3687    3687 
   Size:       6683
 
EcoRV GAT'ATC

Cuts at:   3456    3456 
   Size:       6683
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   4702    4702 
   Size:       6683
 
FspI TGC'GCA

Cuts at:   4464    4464 
   Size:       6683
 
HaeII r_GCGC'y

Cuts at:   2900    4117    4365    5595    6366    2900 
   Size:       1217     248    1230     771    3217

  Fragments arranged by size: 

                3217    1230    1217     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    925     957    1174    1206    1288    1320    1353    1385    1461 
   Size:         32     217      32      82      32      33      32      76

Cuts at:   1461    1493    1979    2011    2196    2228    2291    2323    2389 
   Size:         32     486      32     185      32      63      32      66

Cuts at:   2389    2421    2552    2584    2775    2794    2807    2826    4525 
   Size:         32     131      32     191      19      13      19    1699

Cuts at:   4525    4555    4557    4587     925 
   Size:         30       2      30    3021

  Fragments arranged by size: 

                3021    1699     486     217     191     185     131      82
                  76      66      63      33      32      32      32      32
                  32      32      32      32      32      32      30      30
                  19      19      13       2
 
HincII GTy'rAC

Cuts at:   3722    3722 
   Size:       6683
 
HindIII A'AGCT_T

Cuts at:   2641    2641 
   Size:       6683
 
KasI G'GCGC_C

Cuts at:   2896    4113    4361    2896 
   Size:       1217     248    5218

  Fragments arranged by size: 

                5218    1217     248
 
KpnI G_GTAC'C

Cuts at:   3703    3703 
   Size:       6683
 
MfeI C'AATT_G

Cuts at:    296    2531     296 
   Size:       2235    4448
 
MluI A'CGCG_T

Cuts at:   6116    6116 
   Size:       6683
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2048    2620    2627    5442    5626    2048 
   Size:        572       7    2815     184    3105

  Fragments arranged by size: 

                3105    2815     572     184       7
 
MscI TGG'CCA

Cuts at:   2177    3647    4444    2177 
   Size:       1470     797    4416

  Fragments arranged by size: 

                4416    1470     797
 
MslI CAynn'nnrTG

Cuts at:    112     382    2422    4672    4799     112 
   Size:        270    2040    2250     127    1996

  Fragments arranged by size: 

                2250    2040    1996     270     127
 
MspA1I CmG'CkG

Cuts at:    128     617     830    2230    2940    3615    3794    4108    4468 
   Size:        489     213    1400     710     675     179     314     360

Cuts at:   4468    5252    5497     128 
   Size:        784     245    1314

  Fragments arranged by size: 

                1400    1314     784     710     675     489     360     314
                 245     213     179
 
NaeI GCC'GGC

Cuts at:    586    4864     586 
   Size:       4278    2405
 
NarI GG'CG_CC

Cuts at:   2897    4114    4362    2897 
   Size:       1217     248    5218

  Fragments arranged by size: 

                5218    1217     248
 
NcoI C'CATG_G

Cuts at:    383     597    2825     383 
   Size:        214    2228    4241

  Fragments arranged by size: 

                4241    2228     214
 
NgoMIV G'CCGG_C

Cuts at:    584    4862     584 
   Size:       4278    2405
 
NheI G'CTAG_C

Cuts at:   3037    3037 
   Size:       6683
 
NspI r_CATG'y

Cuts at:   1191    2473    2811    3736    4236    5839    1191 
   Size:       1282     338     925     500    1603    2035

  Fragments arranged by size: 

                2035    1603    1282     925     500     338
 
PciI A'CATG_T

Cuts at:   1187    5835    1187 
   Size:       4648    2035
 
PflMI CCAn_nnn'nTGG

Cuts at:   3493    3493 
   Size:       6683
 
PfoI T'CCnGG_A

Cuts at:   1456    3407    1456 
   Size:       1951    4732
 
PmeI GTTT'AAAC

Cuts at:   3682    3682 
   Size:       6683
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5129    5161    5129 
   Size:         32    6651
 
PpuMI rG'GwC_Cy

Cuts at:    675     949    1029    1730     675 
   Size:        274      80     701    5628

  Fragments arranged by size: 

                5628     701     274      80
 
PsiI TTA'TAA

Cuts at:   1933    1933 
   Size:       6683
 
PspOMI G'GGCC_C

Cuts at:    656     923    1018     656 
   Size:        267      95    6321

  Fragments arranged by size: 

                6321     267      95
 
PstI C_TGCA'G

Cuts at:   3730    4415    3730 
   Size:        685    5998
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6683
 
PvuII CAG'CTG

Cuts at:    617    2230    3615    4108    4468     617 
   Size:       1613    1385     493     360    2832

  Fragments arranged by size: 

                2832    1613    1385     493     360
 
RsrII CG'GwC_CG

Cuts at:   4878    4878 
   Size:       6683
 
SacI G_AGCT'C

Cuts at:   3021    3407    3697    3021 
   Size:        386     290    6007

  Fragments arranged by size: 

                6007     386     290
 
SacII CC_GC'GG

Cuts at:    129     831     129 
   Size:        702    5981
 
SalI G'TCGA_C

Cuts at:   3720    3720 
   Size:       6683
 
SanDI GG'GwC_CC

Cuts at:    949     949 
   Size:       6683
 
SapI GCTCTTCn'nnn_

Cuts at:    176    4706    4916     176 
   Size:       4530     210    1943

  Fragments arranged by size: 

                4530    1943     210
 
SbfI CC_TGCA'GG

Cuts at:   3730    3730 
   Size:       6683
 
ScaI AGT'ACT

Cuts at:   6200    6200 
   Size:       6683
 
SfcI C'TryA_G

Cuts at:     33     570     900    1237    3726    4411    5379    5570      33 
   Size:        537     330     337    2489     685     968     191    1146

  Fragments arranged by size: 

                2489    1146     968     685     537     337     330     191
            
 
SfoI GGC'GCC

Cuts at:   2898    4115    4363    2898 
   Size:       1217     248    5218

  Fragments arranged by size: 

                5218    1217     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6683
 
SmaI CCC'GGG

Cuts at:    320     805    3705     320 
   Size:        485    2900    3298

  Fragments arranged by size: 

                3298    2900     485
 
SmlI C'TyrA_G

Cuts at:   1159    1398    3281    3838    4753    5467    5729    1159 
   Size:        239    1883     557     915     714     262    2113

  Fragments arranged by size: 

                2113    1883     915     714     557     262     239
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6683
 
SphI G_CATG'C

Cuts at:   2473    3736    4236    2473 
   Size:       1263     500    4920

  Fragments arranged by size: 

                4920    1263     500
 
SspI AAT'ATT

Cuts at:   3359    3359 
   Size:       6683
 
StyI C'CwwG_G

Cuts at:    383     597    1003    2825    3095    3817    4171     383 
   Size:        214     406    1822     270     722     354    2895

  Fragments arranged by size: 

                2895    1822     722     406     354     270     214
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1453    1488    2533    4266    4399    4858    5067    1453 
   Size:         35    1045    1733     133     459     209    3069

  Fragments arranged by size: 

                3069    1733    1045     459     209     133      35
 
TatI w'GTAC_w

Cuts at:    200     911    6198     200 
   Size:        711    5287     685

  Fragments arranged by size: 

                5287     711     685
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    233     265    1652    1684    2157    2189    3499    3531    6200 
   Size:         32    1387      32     473      32    1310      32    2669

Cuts at:   6200    6232     233 
   Size:         32     684

  Fragments arranged by size: 

                2669    1387    1310     684     473      32      32      32
                  32      32
 
Tth111I GACn'n_nGTC

Cuts at:   4480    4480 
   Size:       6683
 
XbaI T'CTAG_A

Cuts at:   3714    3714 
   Size:       6683
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    280     501    3339    4178     280 
   Size:        221    2838     839    2785

  Fragments arranged by size: 

                2838    2785     839     221
 
XmaI C'CCGG_G

Cuts at:    318     803    3703     318 
   Size:        485    2900    3298

  Fragments arranged by size: 

                3298    2900     485
 
ZraI GAC'GTC

Cuts at:   1366    1366 
   Size:       6683
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AhdI     AleI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    AvrII    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NheI     NspI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI     PsiI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AgeI     AloI     AscI     BaeI    BfrBI
    BmgBI    BsiWI    BsrGI  BstZ17I     ClaI    CspCI     FseI    FspAI
     HpaI     NdeI     NotI     NruI     NsiI     PacI     PasI     PmlI
    PshAI    PspXI     PsrI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     StuI     SwaI     XhoI     XmnI
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