Restriction map for pF1KB9458
 (Circular) (Six-base) MAPSORT of: pF1KB9458.seq  Check: 8038  from: 1  to: 5624



pF1KB9458  5624 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:30  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1279    2011    2274    1279 
   Size:        732     263    4629

  Fragments arranged by size: 

                4629     732     263
 
AatII G_ACGT'C

Cuts at:    785     785 
   Size:       5624
 
AccI GT'mk_AC

Cuts at:   1424    1559    1850    2662    1424 
   Size:        135     291     812    4386

  Fragments arranged by size: 

                4386     812     291     135
 
Acc65I G'GTAC_C

Cuts at:   2640    2640 
   Size:       5624
 
AclI AA'CG_TT

Cuts at:   5464    5464 
   Size:       5624
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    682    1093    1677    2313    2587    3449    3881    4234    4896 
   Size:        411     584     636     274     862     432     353     662

Cuts at:   4896     682 
   Size:       1410

  Fragments arranged by size: 

                1410     862     662     636     584     432     411     353
                 274
 
AfeI AGC'GCT

Cuts at:    494     494 
   Size:       5624
 
AflII C'TTAA_G

Cuts at:   2242    2242 
   Size:       5624
 
AflIII A'CryG_T

Cuts at:   2976    4776    5057    2976 
   Size:       1800     281    3543

  Fragments arranged by size: 

                3543    1800     281
 
AgeI A'CCGG_T

Cuts at:    306    2318     306 
   Size:       2012    3612
 
AleI CACnn'nnGTG

Cuts at:   1681    1882    2368    1681 
   Size:        201     486    4937

  Fragments arranged by size: 

                4937     486     201
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    979    2296     979 
   Size:       1317    4307
 
AlwNI CAG_nnn'CTG

Cuts at:    419     556    1662    2462    2929    4367     419 
   Size:        137    1106     800     467    1438    1676

  Fragments arranged by size: 

                1676    1438    1106     800     467     137
 
ApaI G_GGCC'C

Cuts at:    513    1232     513 
   Size:        719    4905
 
ApaLI G'TGCA_C

Cuts at:    873    1234    4462     873 
   Size:        361    3228    2035

  Fragments arranged by size: 

                3228    2035     361
 
ApoI r'AATT_y

Cuts at:   2628    4977    5233    2628 
   Size:       2349     256    3019

  Fragments arranged by size: 

                3019    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5624
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5624
 
AvaI C'yCGr_G

Cuts at:    501    1183    2391    2507    2530    2644     501 
   Size:        682    1208     116      23     114    3481

  Fragments arranged by size: 

                3481    1208     682     116     114      23
 
BamHI G'GATC_C

Cuts at:   2649    2649 
   Size:       5624
 
BanI G'GyrC_C

Cuts at:    309    1193    2640    3054    3302    3337     309 
   Size:        884    1447     414     248      35    2596

  Fragments arranged by size: 

                2596    1447     884     414     248      35
 
BanII G_rGCy'C

Cuts at:    502     513    1088    1184    1232    2638    3668     502 
   Size:         11     575      96      48    1406    1030    2458

  Fragments arranged by size: 

                2458    1406    1030     575      96      48      11
 
BbeI G_GCGC'C

Cuts at:   3058    3306    3058 
   Size:        248    5376
 
BbsI GAAGACnn'nnnn_

Cuts at:    360     574    1618    1851    1907    2070    2393    2580     360 
   Size:        214    1044     233      56     163     323     187    3404

  Fragments arranged by size: 

                3404    1044     323     233     214     187     163      56
            
 
BbvCI CC'TCA_GC

Cuts at:   2582    2582 
   Size:       5624
 
BciVI GTATCCnnnnn_n'

Cuts at:   3522    4578    5624    3522 
   Size:       1056    1046    3522

  Fragments arranged by size: 

                3522    1056    1046
 
BclI T'GATC_A

Cuts at:   1439    3144    1439 
   Size:       1705    3919
 
BglII A'GATC_T

Cuts at:   3139    5176    3139 
   Size:       2037    3587
 
BlpI GC'TnA_GC

Cuts at:   2525    2736    2525 
   Size:        211    5413
 
Bme1580I G_kGCm'C

Cuts at:    513     877    1232    1238    3249    3342    4466     513 
   Size:        364     355       6    2011      93    1124    1671

  Fragments arranged by size: 

                2011    1671    1124     364     355      93       6
 
BmgBI CAC'GTC

Cuts at:   1937    1937 
   Size:       5624
 
BmrI ACTGGGnnnn_n'

Cuts at:   2836    2917    3252    5396    2836 
   Size:         81     335    2144    3064

  Fragments arranged by size: 

                3064    2144     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    260    1299    3991     260 
   Size:       1039    2692    1893

  Fragments arranged by size: 

                2692    1893    1039
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    107     118     441     655    1099    1147    1312    1529    1550 
   Size:         11     323     214     444      48     165     217      21

Cuts at:   1550    2029    2065     107 
   Size:        479      36    3666

  Fragments arranged by size: 

                3666     479     444     323     217     214     165      48
                  36      21      11
 
Bpu10I CC'TnA_GC

Cuts at:   2268    2422    2582    2268 
   Size:        154     160    5310

  Fragments arranged by size: 

                5310     160     154
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1687    2800    3679    4393    4691    1687 
   Size:       1113     879     714     298    2620

  Fragments arranged by size: 

                2620    1113     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    106    1312    1820    2567    5328     106 
   Size:       1206     508     747    2761     402

  Fragments arranged by size: 

                2761    1206     747     508     402
 
BsaAI yAC'GTr

Cuts at:   2977    2977 
   Size:       5624
 
BsaBI GATnn'nnATC

Cuts at:      6    1042    1789    3138    5175       6 
   Size:       1036     747    1349    2037     455

  Fragments arranged by size: 

                2037    1349    1036     747     455
 
BsaHI Gr'CG_yC

Cuts at:    782    1108    2408    3055    3303    4005    5501     782 
   Size:        326    1300     647     248     702    1496     905

  Fragments arranged by size: 

                1496    1300     905     702     647     326     248
 
BsaWI w'CCGG_w

Cuts at:    306    2318    2813    3334    4012    4423    4570    4844     306 
   Size:       2012     495     521     678     411     147     274    1086

  Fragments arranged by size: 

                2012    1086     678     521     495     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    105     135     767     797    1171    1201     105 
   Size:         30     632      30     374      30    4528

  Fragments arranged by size: 

                4528     632     374      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    213    1096    1230    1568     213 
   Size:        883     134     338    4269

  Fragments arranged by size: 

                4269     883     338     134
 
BseYI C'CCAG_C

Cuts at:    145     489     495     513     885    1126    3049    3807    4472 
   Size:        344       6      18     372     241    1923     758     665

Cuts at:   4472    4880     145 
   Size:        408     889

  Fragments arranged by size: 

                1923     889     758     665     408     372     344     241
                  18       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    583     703     952    1045    1209     583 
   Size:        120     249      93     164    4998

  Fragments arranged by size: 

                4998     249     164     120      93
 
BsiEI CG_ry'CG

Cuts at:    104     929    1001    1603    3166    3212    4442     104 
   Size:        825      72     602    1563      46    1230    1286

  Fragments arranged by size: 

                1563    1286    1230     825     602      72      46
 
BsiHKAI G_wGCw'C

Cuts at:    877    1088    1238    2508    2638    3416    3606    4466     877 
   Size:        211     150    1270     130     778     190     860    2035

  Fragments arranged by size: 

                2035    1270     860     778     211     190     150     130
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:    289     729     289 
   Size:        440    5184
 
Bsp1286I G_dGCh'C

Cuts at:    502     513     877    1088    1184    1232    1238    2508    2638 
   Size:         11     364     211      96      48       6    1270     130

Cuts at:   2638    3249    3342    3416    3606    3668    4466     502 
   Size:        611      93      74     190      62     798    1660

  Fragments arranged by size: 

                1660    1270     798     611     364     211     190     130
                  96      93      74      62      48      11       6
 
BspHI T'CATG_A

Cuts at:   1802    4056    1802 
   Size:       2254    3370
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    605    1279    2011    2274    2674    2888    3190    3571     605 
   Size:        674     732     263     400     214     302     381    2658

  Fragments arranged by size: 

                2658     732     674     400     381     302     263     214
            
 
BsrBI CCG'CTC

Cuts at:     50    2267    3916    3970    5454    5619      50 
   Size:       2217    1649      54    1484     165      55

  Fragments arranged by size: 

                2217    1649    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2138    3536    2138 
   Size:       1398    4226
 
BsrFI r'CCGG_y

Cuts at:    306    2318    3622    3803     306 
   Size:       2012    1304     181    2127

  Fragments arranged by size: 

                2127    2012    1304     181
 
BsrGI T'GTAC_A

Cuts at:   1028    1433    1028 
   Size:        405    5219
 
BssSI C'ACGA_G

Cuts at:    225    3895    4603     225 
   Size:       3670     708    1246

  Fragments arranged by size: 

                3670    1246     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3001    3001 
   Size:       5624
 
BstBI TT'CG_AA

Cuts at:   3985    3985 
   Size:       5624
 
BstEII G'GTnAC_C

Cuts at:    753     753 
   Size:       5624
 
BstXI CCAn_nnnn'nTGG

Cuts at:    885    1884    2007    2034     885 
   Size:        999     123      27    4475

  Fragments arranged by size: 

                4475     999     123      27
 
BstYI r'GATC_y

Cuts at:      1     416    1172    1535    2649    3116    3139    3474    3720 
   Size:        415     756     363    1114     467      23     335     246

Cuts at:   3720    4026    4038    4124    5176       1 
   Size:        306      12      86    1052     449

  Fragments arranged by size: 

                1114    1052     756     467     449     415     363     335
                 306     246      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    360     360 
   Size:       5624
 
BtgI C'CryG_G

Cuts at:    107     154     798    1451    1472    1679    1721    1770     107 
   Size:         47     644     653      21     207      42      49    3961

  Fragments arranged by size: 

                3961     653     644     207      49      47      42      21
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    708    3574    3755    3918     708 
   Size:       2866     181     163    2414

  Fragments arranged by size: 

                2866    2414     181     163
 
BtsI GCAGTG_nn'

Cuts at:    715    2867     715 
   Size:       2152    3472
 
DraI TTT'AAA

Cuts at:   2623    2623 
   Size:       5624
 
DraIII CAC_nnn'GTG

Cuts at:   1217    1217 
   Size:       5624
 
DrdI GACnn_nn'nnGTC

Cuts at:   1390    2584    3330    4674    1390 
   Size:       1194     746    1344    2340

  Fragments arranged by size: 

                2340    1344    1194     746
 
EaeI y'GGCC_r

Cuts at:    157     866     926     986     998    1600    1673    3209    3383 
   Size:        709      60      60      12     602      73    1536     174

Cuts at:   3383    3774    3801    5146     157 
   Size:        391      27    1345     635

  Fragments arranged by size: 

                1536    1345     709     635     602     391     174      73
                  60      60      27      12
 
EagI C'GGCC_G

Cuts at:    926     998    1600    3209     926 
   Size:         72     602    1609    3341

  Fragments arranged by size: 

                3341    1609     602      72
 
EarI CTCTTCn'nnn_

Cuts at:    441    2227    3647    3857     441 
   Size:       1786    1420     210    2208

  Fragments arranged by size: 

                2208    1786    1420     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3832    4574    4720    5434    3832 
   Size:        742     146     714    4022

  Fragments arranged by size: 

                4022     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1086    2636    1086 
   Size:       1550    4074
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    107     118     441     655     682    1093    1099    1147    1312 
   Size:         11     323     214      27     411       6      48     165

Cuts at:   1312    1529    1550    1677    2029    2065    2313    2587    3449 
   Size:        217      21     127     352      36     248     274     862

Cuts at:   3449    3881    4234    4896     107 
   Size:        432     353     662     835

  Fragments arranged by size: 

                 862     835     662     432     411     353     352     323
                 274     248     217     214     165     127      48      36
                  27      21      11       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:    560     979    1464     560 
   Size:        419     485    4720

  Fragments arranged by size: 

                4720     485     419
 
EcoO109I rG'GnC_Cy

Cuts at:    509     510    1954    1967    2763     509 
   Size:          1    1444      13     796    3370

  Fragments arranged by size: 

                3370    1444     796      13       1
 
EcoRI G'AATT_C

Cuts at:   2628    2628 
   Size:       5624
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1419    2094    3643    1419 
   Size:        675    1549    3400

  Fragments arranged by size: 

                3400    1549     675
 
FspI TGC'GCA

Cuts at:   3405    3405 
   Size:       5624
 
HaeII r_GCGC'y

Cuts at:    496     941    3058    3306    4536    5307     496 
   Size:        445    2117     248    1230     771     813

  Fragments arranged by size: 

                2117    1230     813     771     445     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    105     137     767     799    1311    1343    1525    1557    1921 
   Size:         32     630      32     512      32     182      32     364

Cuts at:   1921    1953    2503    2535    3466    3496    3498    3528     105 
   Size:         32     550      32     931      30       2      30    2201

  Fragments arranged by size: 

                2201     931     630     550     512     364     182      32
                  32      32      32      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    675    2163    2663     675 
   Size:       1488     500    3636

  Fragments arranged by size: 

                3636    1488     500
 
KasI G'GCGC_C

Cuts at:   3054    3302    3054 
   Size:        248    5376
 
KpnI G_GTAC'C

Cuts at:   2644    2644 
   Size:       5624
 
MluI A'CGCG_T

Cuts at:   5057    5057 
   Size:       5624
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   4383    4567    4383 
   Size:        184    5440
 
MscI TGG'CCA

Cuts at:   1675    3385    1675 
   Size:       1710    3914
 
MslI CAynn'nnrTG

Cuts at:   1681    1882    2032    2368    3613    3740    1681 
   Size:        201     150     336    1245     127    3565

  Fragments arranged by size: 

                3565    1245     336     201     150     127
 
MspA1I CmG'CkG

Cuts at:    885    1113    1126    1167    1356    1772    1882    2735    3049 
   Size:        228      13      41     189     416     110     853     314

Cuts at:   3049    3409    4193    4438     885 
   Size:        360     784     245    2071

  Fragments arranged by size: 

                2071     853     784     416     360     314     245     228
                 189     110      41      13
 
NaeI GCC'GGC

Cuts at:   3805    3805 
   Size:       5624
 
NarI GG'CG_CC

Cuts at:   3055    3303    3055 
   Size:        248    5376
 
NcoI C'CATG_G

Cuts at:    107     798    1451    1721     107 
   Size:        691     653     270    4010

  Fragments arranged by size: 

                4010     691     653     270
 
NgoMIV G'CCGG_C

Cuts at:   3803    3803 
   Size:       5624
 
NspI r_CATG'y

Cuts at:   1029    1341    1758    2136    2677    3177    4780    1029 
   Size:        312     417     378     541     500    1603    1873

  Fragments arranged by size: 

                1873    1603     541     500     417     378     312
 
PciI A'CATG_T

Cuts at:   4776    4776 
   Size:       5624
 
PflMI CCAn_nnn'nTGG

Cuts at:    419    1785     419 
   Size:       1366    4258
 
PfoI T'CCnGG_A

Cuts at:    632    1175    1201    2219     632 
   Size:        543      26    1018    4037

  Fragments arranged by size: 

                4037    1018     543      26
 
PmeI GTTT'AAAC

Cuts at:   2623    2623 
   Size:       5624
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4070    4102    4070 
   Size:         32    5592
 
PspOMI G'GGCC_C

Cuts at:    509    1228     509 
   Size:        719    4905
 
PstI C_TGCA'G

Cuts at:    445     911    1373    1595    1658    1966    2204    2285    2671 
   Size:        466     462     222      63     308     238      81     386

Cuts at:   2671    3356     445 
   Size:        685    2713

  Fragments arranged by size: 

                2713     685     466     462     386     308     238     222
                  81      63
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5624
 
PvuII CAG'CTG

Cuts at:    885    1126    3049    3409     885 
   Size:        241    1923     360    3100

  Fragments arranged by size: 

                3100    1923     360     241
 
RsrII CG'GwC_CG

Cuts at:   3819    3819 
   Size:       5624
 
SacI G_AGCT'C

Cuts at:   1088    2638    1088 
   Size:       1550    4074
 
SacII CC_GC'GG

Cuts at:   1773    1773 
   Size:       5624
 
SalI G'TCGA_C

Cuts at:   2661    2661 
   Size:       5624
 
SapI GCTCTTCn'nnn_

Cuts at:    441    2227    3647    3857     441 
   Size:       1786    1420     210    2208

  Fragments arranged by size: 

                2208    1786    1420     210
 
SbfI CC_TGCA'GG

Cuts at:   1373    1966    2671    1373 
   Size:        593     705    4326

  Fragments arranged by size: 

                4326     705     593
 
ScaI AGT'ACT

Cuts at:   5141    5141 
   Size:       5624
 
SfcI C'TryA_G

Cuts at:     33     441     907    1305    1369    1464    1591    1632    1654 
   Size:        408     466     398      64      95     127      41      22

Cuts at:   1654    1962    2200    2281    2667    3352    4320    4511      33 
   Size:        308     238      81     386     685     968     191    1146

  Fragments arranged by size: 

                1146     968     685     466     408     398     386     308
                 238     191     127      95      81      64      41      22
            
 
SfoI GGC'GCC

Cuts at:   3056    3304    3056 
   Size:        248    5376
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5624
 
SgrAI Cr'CCGG_yG

Cuts at:    306     306 
   Size:       5624
 
SmaI CCC'GGG

Cuts at:   2393    2646    2393 
   Size:        253    5371
 
SmlI C'TyrA_G

Cuts at:   1702    2242    2507    2779    3694    4408    4670    1702 
   Size:        540     265     272     915     714     262    2656

  Fragments arranged by size: 

                2656     915     714     540     272     265     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5624
 
SphI G_CATG'C

Cuts at:   2677    3177    2677 
   Size:        500    5124
 
StyI C'CwwG_G

Cuts at:    107     798    1451    1721    1739    2758    3112     107 
   Size:        691     653     270      18    1019     354    2619

  Fragments arranged by size: 

                2619    1019     691     653     354     270      18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2140    3207    3340    3799    4008    2140 
   Size:       1067     133     459     209    3756

  Fragments arranged by size: 

                3756    1067     459     209     133
 
TatI w'GTAC_w

Cuts at:   1028    1433    1688    1994    5139    1028 
   Size:        405     255     306    3145    1513

  Fragments arranged by size: 

                3145    1513     405     306     255
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1171    1203    5141    5173    1171 
   Size:         32    3938      32    1622

  Fragments arranged by size: 

                3938    1622      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    333    3421     333 
   Size:       3088    2536
 
XbaI T'CTAG_A

Cuts at:   2655    2655 
   Size:       5624
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    948    2217    3119     948 
   Size:       1269     902    3453

  Fragments arranged by size: 

                3453    1269     902
 
XhoI C'TCGA_G

Cuts at:   2507    2507 
   Size:       5624
 
XmaI C'CCGG_G

Cuts at:   2391    2644    2391 
   Size:        253    5371
 
XmnI GAAnn'nnTTC

Cuts at:   2236    2260    2236 
   Size:         24    5600
 
ZraI GAC'GTC

Cuts at:    783     783 
   Size:       5624
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI    PflMI
     PfoI     PmeI     PpiI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI    SgrAI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AhdI     AloI     AscI    AvrII     BaeI     BcgI    BfrBI     BglI
     BmtI    BsiWI     BsmI    BspEI   BssHII  BstZ17I     ClaI    CspCI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PmlI    PpuMI    PshAI
     PsiI    PspXI     PsrI    SanDI    SexAI     SfiI    SnaBI     SrfI
     SspI     StuI     SwaI
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