Restriction map for pF1KB9458
 (Linear) (Six-base) MAPSORT of: KIBB9458.seq  Check: 3640  from: 1  to: 2511



KIBB9458.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1171    1903    2166    2511 
   Size:       1171     732     263     345

  Fragments arranged by size: 

                1171     732     345     263
 
AatII G_ACGT'C

Cuts at:      0     677    2511 
   Size:        677    1834
 
AccI GT'mk_AC

Cuts at:      0    1316    1451    1742    2511 
   Size:       1316     135     291     769

  Fragments arranged by size: 

                1316     769     291     135
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     574     985    1569    2205    2479    2511 
   Size:        574     411     584     636     274      32

  Fragments arranged by size: 

                 636     584     574     411     274      32
 
AfeI AGC'GCT

Cuts at:      0     386    2511 
   Size:        386    2125
 
AflII C'TTAA_G

Cuts at:      0    2134    2511 
   Size:       2134     377
 
AgeI A'CCGG_T

Cuts at:      0     198    2210    2511 
   Size:        198    2012     301

  Fragments arranged by size: 

                2012     301     198
 
AleI CACnn'nnGTG

Cuts at:      0    1573    1774    2260    2511 
   Size:       1573     201     486     251

  Fragments arranged by size: 

                1573     486     251     201
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     871    2188    2511 
   Size:        871    1317     323

  Fragments arranged by size: 

                1317     871     323
 
AlwNI CAG_nnn'CTG

Cuts at:      0     311     448    1554    2354    2511 
   Size:        311     137    1106     800     157

  Fragments arranged by size: 

                1106     800     311     157     137
 
ApaI G_GGCC'C

Cuts at:      0     405    1124    2511 
   Size:        405     719    1387

  Fragments arranged by size: 

                1387     719     405
 
ApaLI G'TGCA_C

Cuts at:      0     765    1126    2511 
   Size:        765     361    1385

  Fragments arranged by size: 

                1385     765     361
 
AvaI C'yCGr_G

Cuts at:      0     393    1075    2283    2399    2422    2511 
   Size:        393     682    1208     116      23      89

  Fragments arranged by size: 

                1208     682     393     116      89      23
 
BanI G'GyrC_C

Cuts at:      0     201    1085    2511 
   Size:        201     884    1426

  Fragments arranged by size: 

                1426     884     201
 
BanII G_rGCy'C

Cuts at:      0     394     405     980    1076    1124    2511 
   Size:        394      11     575      96      48    1387

  Fragments arranged by size: 

                1387     575     394      96      48      11
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     252     466    1510    1743    1799    1962    2285    2472 
   Size:        252     214    1044     233      56     163     323     187

Cuts at:   2472    2511 
   Size:         39

  Fragments arranged by size: 

                1044     323     252     233     214     187     163      56
                  39
 
BbvCI CC'TCA_GC

Cuts at:      0    2474    2511 
   Size:       2474      37
 
BclI T'GATC_A

Cuts at:      0    1331    2511 
   Size:       1331    1180
 
BlpI GC'TnA_GC

Cuts at:      0    2417    2511 
   Size:       2417      94
 
Bme1580I G_kGCm'C

Cuts at:      0     405     769    1124    1130    2511 
   Size:        405     364     355       6    1381

  Fragments arranged by size: 

                1381     405     364     355       6
 
BmgBI CAC'GTC

Cuts at:      0    1829    2511 
   Size:       1829     682
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     152    1191    2511 
   Size:        152    1039    1320

  Fragments arranged by size: 

                1320    1039     152
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      10     333     547     991    1039    1204    1421    1442 
   Size:         10     323     214     444      48     165     217      21

Cuts at:   1442    1921    1957    2511 
   Size:        479      36     554

  Fragments arranged by size: 

                 554     479     444     323     217     214     165      48
                  36      21      10
 
Bpu10I CC'TnA_GC

Cuts at:      0    2160    2314    2474    2511 
   Size:       2160     154     160      37

  Fragments arranged by size: 

                2160     160     154      37
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1579    2511 
   Size:       1579     932
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1204    1712    2459    2511 
   Size:       1204     508     747      52

  Fragments arranged by size: 

                1204     747     508      52
 
BsaBI GATnn'nnATC

Cuts at:      0     934    1681    2511 
   Size:        934     747     830

  Fragments arranged by size: 

                 934     830     747
 
BsaHI Gr'CG_yC

Cuts at:      0     674    1000    2300    2511 
   Size:        674     326    1300     211

  Fragments arranged by size: 

                1300     674     326     211
 
BsaWI w'CCGG_w

Cuts at:      0     198    2210    2511 
   Size:        198    2012     301

  Fragments arranged by size: 

                2012     301     198
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      27     659     689    1063    1093    2511 
   Size:         27     632      30     374      30    1418

  Fragments arranged by size: 

                1418     632     374      30      30      27
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     105     988    1122    1460    2511 
   Size:        105     883     134     338    1051

  Fragments arranged by size: 

                1051     883     338     134     105
 
BseYI C'CCAG_C

Cuts at:      0      37     381     387     405     777    1018    2511 
   Size:         37     344       6      18     372     241    1493

  Fragments arranged by size: 

                1493     372     344     241      37      18       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     475     595     844     937    1101    2511 
   Size:        475     120     249      93     164    1410

  Fragments arranged by size: 

                1410     475     249     164     120      93
 
BsiEI CG_ry'CG

Cuts at:      0     821     893    1495    2511 
   Size:        821      72     602    1016

  Fragments arranged by size: 

                1016     821     602      72
 
BsiHKAI G_wGCw'C

Cuts at:      0     769     980    1130    2400    2511 
   Size:        769     211     150    1270     111

  Fragments arranged by size: 

                1270     769     211     150     111
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     181     621    2511 
   Size:        181     440    1890

  Fragments arranged by size: 

                1890     440     181
 
Bsp1286I G_dGCh'C

Cuts at:      0     394     405     769     980    1076    1124    1130    2400 
   Size:        394      11     364     211      96      48       6    1270

Cuts at:   2400    2511 
   Size:        111

  Fragments arranged by size: 

                1270     394     364     211     111      96      48      11
                   6
 
BspHI T'CATG_A

Cuts at:      0    1694    2511 
   Size:       1694     817
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     497    1171    1903    2166    2511 
   Size:        497     674     732     263     345

  Fragments arranged by size: 

                 732     674     497     345     263
 
BsrBI CCG'CTC

Cuts at:      0    2159    2511 
   Size:       2159     352
 
BsrDI GCAATG_nn'

Cuts at:      0    2030    2511 
   Size:       2030     481
 
BsrFI r'CCGG_y

Cuts at:      0     198    2210    2511 
   Size:        198    2012     301

  Fragments arranged by size: 

                2012     301     198
 
BsrGI T'GTAC_A

Cuts at:      0     920    1325    2511 
   Size:        920     405    1186

  Fragments arranged by size: 

                1186     920     405
 
BssSI C'ACGA_G

Cuts at:      0     117    2511 
   Size:        117    2394
 
BstEII G'GTnAC_C

Cuts at:      0     645    2511 
   Size:        645    1866
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     777    1776    1899    1926    2511 
   Size:        777     999     123      27     585

  Fragments arranged by size: 

                 999     777     585     123      27
 
BstYI r'GATC_y

Cuts at:      0     308    1064    1427    2511 
   Size:        308     756     363    1084

  Fragments arranged by size: 

                1084     756     363     308
 
Bsu36I CC'TnA_GG

Cuts at:      0     252    2511 
   Size:        252    2259
 
BtgI C'CryG_G

Cuts at:      0      46     690    1343    1364    1571    1613    1662    2511 
   Size:         46     644     653      21     207      42      49     849

  Fragments arranged by size: 

                 849     653     644     207      49      46      42      21
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     600    2511 
   Size:        600    1911
 
BtsI GCAGTG_nn'

Cuts at:      0     607    2511 
   Size:        607    1904
 
DraIII CAC_nnn'GTG

Cuts at:      0    1109    2511 
   Size:       1109    1402
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1282    2476    2511 
   Size:       1282    1194      35

  Fragments arranged by size: 

                1282    1194      35
 
EaeI y'GGCC_r

Cuts at:      0      49     758     818     878     890    1492    1565    2511 
   Size:         49     709      60      60      12     602      73     946

  Fragments arranged by size: 

                 946     709     602      73      60      60      49      12
            
 
EagI C'GGCC_G

Cuts at:      0     818     890    1492    2511 
   Size:        818      72     602    1019

  Fragments arranged by size: 

                1019     818     602      72
 
EarI CTCTTCn'nnn_

Cuts at:      0     333    2119    2511 
   Size:        333    1786     392

  Fragments arranged by size: 

                1786     392     333
 
EcoICRI GAG'CTC

Cuts at:      0     978    2511 
   Size:        978    1533
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      10     333     547     574     985     991    1039    1204 
   Size:         10     323     214      27     411       6      48     165

Cuts at:   1204    1421    1442    1569    1921    1957    2205    2479    2511 
   Size:        217      21     127     352      36     248     274      32

  Fragments arranged by size: 

                 411     352     323     274     248     217     214     165
                 127      48      36      32      27      21      10       6
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     452     871    1356    2511 
   Size:        452     419     485    1155

  Fragments arranged by size: 

                1155     485     452     419
 
EcoO109I rG'GnC_Cy

Cuts at:      0     401     402    1846    1859    2511 
   Size:        401       1    1444      13     652

  Fragments arranged by size: 

                1444     652     401      13       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1311    1986    2511 
   Size:       1311     675     525

  Fragments arranged by size: 

                1311     675     525
 
HaeII r_GCGC'y

Cuts at:      0     388     833    2511 
   Size:        388     445    1678

  Fragments arranged by size: 

                1678     445     388
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      29     659     691    1203    1235    1417    1449    1813 
   Size:         29     630      32     512      32     182      32     364

Cuts at:   1813    1845    2395    2427    2511 
   Size:         32     550      32      84

  Fragments arranged by size: 

                 630     550     512     364     182      84      32      32
                  32      32      32      29
 
HincII GTy'rAC

Cuts at:      0     567    2055    2511 
   Size:        567    1488     456

  Fragments arranged by size: 

                1488     567     456
 
MscI TGG'CCA

Cuts at:      0    1567    2511 
   Size:       1567     944
 
MslI CAynn'nnrTG

Cuts at:      0    1573    1774    1924    2260    2511 
   Size:       1573     201     150     336     251

  Fragments arranged by size: 

                1573     336     251     201     150
 
MspA1I CmG'CkG

Cuts at:      0     777    1005    1018    1059    1248    1664    1774    2511 
   Size:        777     228      13      41     189     416     110     737

  Fragments arranged by size: 

                 777     737     416     228     189     110      41      13
            
 
NcoI C'CATG_G

Cuts at:      0     690    1343    1613    2511 
   Size:        690     653     270     898

  Fragments arranged by size: 

                 898     690     653     270
 
NspI r_CATG'y

Cuts at:      0     921    1233    1650    2028    2511 
   Size:        921     312     417     378     483

  Fragments arranged by size: 

                 921     483     417     378     312
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     311    1677    2511 
   Size:        311    1366     834

  Fragments arranged by size: 

                1366     834     311
 
PfoI T'CCnGG_A

Cuts at:      0     524    1067    1093    2111    2511 
   Size:        524     543      26    1018     400

  Fragments arranged by size: 

                1018     543     524     400      26
 
PspOMI G'GGCC_C

Cuts at:      0     401    1120    2511 
   Size:        401     719    1391

  Fragments arranged by size: 

                1391     719     401
 
PstI C_TGCA'G

Cuts at:      0     337     803    1265    1487    1550    1858    2096    2177 
   Size:        337     466     462     222      63     308     238      81

Cuts at:   2177    2511 
   Size:        334

  Fragments arranged by size: 

                 466     462     337     334     308     238     222      81
                  63
 
PvuII CAG'CTG

Cuts at:      0     777    1018    2511 
   Size:        777     241    1493

  Fragments arranged by size: 

                1493     777     241
 
SacI G_AGCT'C

Cuts at:      0     980    2511 
   Size:        980    1531
 
SacII CC_GC'GG

Cuts at:      0    1665    2511 
   Size:       1665     846
 
SapI GCTCTTCn'nnn_

Cuts at:      0     333    2119    2511 
   Size:        333    1786     392

  Fragments arranged by size: 

                1786     392     333
 
SbfI CC_TGCA'GG

Cuts at:      0    1265    1858    2511 
   Size:       1265     593     653

  Fragments arranged by size: 

                1265     653     593
 
SfcI C'TryA_G

Cuts at:      0     333     799    1197    1261    1356    1483    1524    1546 
   Size:        333     466     398      64      95     127      41      22

Cuts at:   1546    1854    2092    2173    2511 
   Size:        308     238      81     338

  Fragments arranged by size: 

                 466     398     338     333     308     238     127      95
                  81      64      41      22
 
SgrAI Cr'CCGG_yG

Cuts at:      0     198    2511 
   Size:        198    2313
 
SmaI CCC'GGG

Cuts at:      0    2285    2511 
   Size:       2285     226
 
SmlI C'TyrA_G

Cuts at:      0    1594    2134    2399    2511 
   Size:       1594     540     265     112

  Fragments arranged by size: 

                1594     540     265     112
 
StyI C'CwwG_G

Cuts at:      0     690    1343    1613    1631    2511 
   Size:        690     653     270      18     880

  Fragments arranged by size: 

                 880     690     653     270      18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2032    2511 
   Size:       2032     479
 
TatI w'GTAC_w

Cuts at:      0     920    1325    1580    1886    2511 
   Size:        920     405     255     306     625

  Fragments arranged by size: 

                 920     625     405     306     255
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1063    1095    2511 
   Size:       1063      32    1416

  Fragments arranged by size: 

                1416    1063      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     225    2511 
   Size:        225    2286
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     840    2109    2511 
   Size:        840    1269     402

  Fragments arranged by size: 

                1269     840     402
 
XhoI C'TCGA_G

Cuts at:      0    2399    2511 
   Size:       2399     112
 
XmaI C'CCGG_G

Cuts at:      0    2283    2511 
   Size:       2283     228
 
XmnI GAAnn'nnTTC

Cuts at:      0    2128    2152    2511 
   Size:       2128      24     359

  Fragments arranged by size: 

                2128     359      24
 
ZraI GAC'GTC

Cuts at:      0     675    2511 
   Size:        675    1836
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI    AflII     AgeI     AleI
     AlfI    AlwNI     ApaI    ApaLI     AvaI     BanI    BanII     BbsI
    BbvCI     BclI     BlpI Bme1580I    BmgBI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI     EagI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII
     MscI     MslI   MspA1I     NcoI     NspI    PflMI     PfoI   PspOMI
     PstI    PvuII     SacI    SacII     SapI     SbfI     SfcI    SgrAI
     SmaI     SmlI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI   AflIII     AhdI     AloI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI    BfrBI
     BglI    BglII     BmrI     BmtI    BsaAI    BsiWI     BsmI    BspEI
   BssHII   BstAPI    BstBI  BstZ17I     ClaI    CspCI     DraI     EciI
    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SalI    SanDI     ScaI    SexAI     SfiI     SfoI     SgfI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     XbaI
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