Restriction map for pF1KB9454
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AvaI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BcgI        
BciVI       BclI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BseAI       
BsiEI       BsiWI       BsmBI       BsoBI       BspDI       
BspEI       BspHI       BsrBI       BsrDI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
ClaI        CpoI        DraII       DraIII      DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MspA1I      MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SpeI        
SphI        Sse8387I    SspI        SwaI        Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI           49  GTCTAC               GT^[AC][GT]AC
AciI          198  GCGG                 CCGC(-3/-1)
AcsI          450  AAATTT               [GA]^AATT[CT]
AflIII       1069  ACATGT               A^C[GA][CT]GT
ApoI          450  AAATTT               [GA]^AATT[CT]
Asp700I       544  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          829  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         805  GGACC                G^G[AT]CC
BbvCI         784  GCTGAGG              CCTCAGC(-5/-2)
BcnI         1082  CCCGG                CC^[GC]GG
BfaI          982  CTAG                 C^TAG
BsaAI        1228  TACGTA               [CT]AC^GT[GA]
BsaI          827  GAGACC               GGTCTC(1/5)
BsaWI           6  TCCGGT               [AT]^CCGG[AT]
BsgI          665  GTGCAG               GTGCAG(16/14)
BsmI          997  GAATGC               GAATGC(1/-1)
Bsp1407I      507  TGTACA               T^GTACA
BspLU11I     1069  ACATGT               A^CATGT
BspMI         667  GCAGGT               ACCTGC(4/8)
BsrGI         507  TGTACA               T^GTACA
Cfr13I        805  GGACC                G^G[ACGT]CC
DraI           88  TTTAAA               TTT^AAA
DrdI         1456  GACAATGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I       279  CCTTGG               C^C[AT][AT]GG
HaeIII        814  GGCC                 GG^CC
MaeI          982  CTAG                 C^TAG
NciI         1082  CCCGG                CC^[GC]GG
NspI         1069  ACATGT               [GA]CATG^[CT]
PflFI         829  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacI         1358  GAGCTC               GAGCT^C
Sau96I        805  GGACC                G^G[ACGT]CC
SnaBI        1228  TACGTA               TAC^GTA
SspBI         507  TGTACA               T^GTACA
StuI          813  AGGCCT               AGG^CCT
StyI          279  CCTTGG               C^C[AT][AT]GG
TaqI          552  TCGA                 T^CGA
Tth111I       829  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       552  TCGA                 T^CGA
XcmI         1520  CCAATGTGACCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          544  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AspHI         766  GTGCTC               G[AT]GC[AT]^C
             1358  GAGCTC              
BanII         907  GAGCCC               G[GA]GC[CT]^C
             1358  GAGCTC              
BbsI          103  GAAGAC               GAAGAC(2/6)
             1073  GTCTTC              
BglII        1305  AGATCT               A^GATCT
             1470  AGATCT              
BpmI          593  CTGGAG               CTGGAG(16/14)
              755  CTGGAG              
BpuAI         103  GAAGAC               GAAGAC(2/6)
             1073  GTCTTC              
BsiHKAI       766  GTGCTC               G[AT]GC[AT]^C
             1358  GAGCTC              
BsmFI         192  GGGAC                GGGAC(10/14)
             1533  GGGAC               
BstYI        1305  AGATCT               [GA]^GATC[CT]
             1470  AGATCT              
HapII           7  CCGG                 C^CGG
             1083  CCGG                
HpaII           7  CCGG                 C^CGG
             1083  CCGG                
MaeII        1229  ACGT                 A^CGT
             1284  ACGT                
MflI         1305  AGATCT               [GA]^GATC[CT]
             1470  AGATCT              
MslI           94  CATTCTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1020  CACAGATGTG          
MspI            7  CCGG                 C^CGG
             1083  CCGG                
NlaIV         438  GGTTCC               GG[ACGT]^[ACGT]CC
              805  GGACCC              
PshAI          42  GACTGTCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1326  GACATTTGTC          
SfcI          844  CTACAG               C^T[GA][CT]AG
             1038  CTACAG              
SmlI         1103  CTCAAG               C^T[CT][GA]AG
             1140  CTCAAG              
TfiI            3  GATTC                G^A[AT]TC
             1410  GATTC               
Tsp45I        835  GTCAC                ^GT[GC]AC
             1525  GTGAC               
XhoII        1305  AGATCT               [GA]^GATC[CT]
             1470  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AfaI        AluI        BbvI        BmyI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       DdeI        
DpnII       EarI        EcoRII      Fnu4HI      FokI        
HinfI       HphI        ItaI        Ksp632I     MaeIII      
MboI        MboII       MnlI        MseI        MvaI        
MwoI        NdeII       NlaIII      PleI        RsaI        
Sau3AI      ScrFI       SfaNI       Tru9I       TseI        
Tsp509I     TspRI       
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