Restriction map for pF1KB9446
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       AccI        AccII       AclI        AflII       
AflIII      AgeI        Alw44I      AlwNI       AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
AspHI       AspI        AviII       AvrII       BalI        
BamHI       BbrPI       BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaBI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EaeI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HgaI        HincII      HindII      
HindIII     HpaI        KspI        MaeI        MaeIII      
MamI        MfeI        MluI        MluNI       MscI        
MspA1I      MunI        MvnI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacI        
SacII       SalI        SbfI        SexAI       SfcI        
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

Acc65I        688  GGTACC               G^GTACC
AccIII        171  TCCGGA               T^CCGGA
AciI          637  GCGG                 CCGC(-3/-1)
AcyI            3  GGCGCC               G[GA]^CG[CT]C
AhdI          291  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          421  GATCC                GGATC(4/5)
ApaI          410  GGGCCC               GGGCC^C
Asp718I       688  GGTACC               G^GTACC
AspEI         291  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          413  CCCGGG               C^[CT]CG[GA]G
AvaII          15  GGACC                G^G[AT]CC
BanII         410  GGGCCC               G[GA]GC[CT]^C
BbeI            3  GGCGCC               GGCGC^C
BbsI          288  GAAGAC               GAAGAC(2/6)
BbvCI         388  CCTCAGC              CCTCAGC(-5/-2)
BbvI          274  GCTGC                GCAGC(8/12)
BcgI           46  CGAGTGCTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI          410  GGGCCC               G[AGT]GC[ACT]^C
BpuAI         288  GAAGAC               GAAGAC(2/6)
BsaAI         193  TACGTG               [CT]AC^GT[GA]
BsaHI           3  GGCGCC               G[GA]^CG[CT]C
BsaWI         171  TCCGGA               [AT]^CCGG[AT]
BseAI         171  TCCGGA               T^CCGGA
BsiYI         352  CCTGGAAATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          352  CCTGGAAATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI         413  CCCGGG               C^[CT]CG[GA]G
Bsp1286I      410  GGGCCC               G[AGT]GC[ACT]^C
BspEI         171  TCCGGA               T^CCGGA
BspHI         449  TCATGA               T^CATGA
BsrDI         814  GCAATG               GCAATG(2/0)
BssSI         608  CTCGTG               CACGAG(-5/-1)
BstXI         765  CCAGGTGTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         420  AGATCC               [GA]^GATC[CT]
Cac8I         922  GCCAGC               GC[ACGT]^[ACGT]GC
DsaI          496  CCGTGG               C^C[GA][CT]GG
Eam1105I      291  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI         900  GAATTC               G^AATTC
Fnu4HI        274  GCTGC                GC^[ACGT]GC
Hin1I           3  GGCGCC               G[GA]^CG[CT]C
HphI           40  GGTGA                GGTGA(8/7)
ItaI          274  GCTGC                GC^[ACGT]GC
KasI            3  GGCGCC               G^GCGCC
KpnI          688  GGTACC               GGTAC^C
MflI          420  AGATCC               [GA]^GATC[CT]
MroI          171  TCCGGA               T^CCGGA
NarI            3  GGCGCC               GG^CGCC
PpuMI          14  AGGACCC              [GA]G^G[AT]CC[CT]
RcaI          449  TCATGA               T^CATGA
ScaI          183  AGTACT               AGT^ACT
SfaNI         751  GATGC                GCATC(5/9)
SfoI            3  GGCGCC               GGC^GCC
SmaI          413  CCCGGG               CCC^GGG
SmlI          580  CTTGAG               C^T[CT][GA]AG
TseI          274  GCTGC                G^C[AT]GC
XhoII         420  AGATCC               [GA]^GATC[CT]
XmaCI         413  CCCGGG               C^CCGGG
XmaI          413  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

Asp700I       120  GAAATACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              163  GAATGGGTTC          
BanI            3  GGCGCC               G^G[CT][GA]CC
              688  GGTACC              
BcnI          413  CCCGG                CC^[GC]GG
              414  CCGGG               
BsaI          382  GAGACC               GGTCTC(1/5)
              714  GAGACC              
BseRI         395  CTCCTC               GAGGAG(10/8)
              941  CTCCTC              
BsmAI         382  GAGAC                GTCTC(1/5)
              714  GAGAC               
BsmFI         515  GGGAC                GGGAC(10/14)
              762  GTCCC               
BsrI          512  ACTGG                ACTGG(1/-1)
              377  CCAGT               
CfoI            4  GCGC                 GCG^C
              778  GCGC                
DpnII         421  GATC                 ^GATC
              742  GATC                
DraII          14  AGGACCC              [GA]G^G[ACGT]CC[CT]
              409  AGGGCCC             
EcoO109I       14  AGGACCC              [GA]G^G[ACGT]CC[CT]
              409  AGGGCCC             
HaeII           3  GGCGCC               [GA]GCGC^[CT]
              777  AGCGCC              
HaeIII         65  GGCC                 GG^CC
              411  GGCC                
HapII         172  CCGG                 C^CGG
              414  CCGG                
HhaI            4  GCGC                 GCG^C
              778  GCGC                
HinP1I          4  GCGC                 G^CGC
              778  GCGC                
HinfI         477  GATTC                G^A[ACGT]TC
              571  GATTC               
HpaII         172  CCGG                 C^CGG
              414  CCGG                
MaeII         194  ACGT                 A^CGT
              677  ACGT                
MboI          421  GATC                 ^GATC
              742  GATC                
MslI          133  CATTACAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              745  CATCCCGATG          
MspI          172  CCGG                 C^CGG
              414  CCGG                
NciI          413  CCCGG                CC^[GC]GG
              414  CCGGG               
NdeII         421  GATC                 ^GATC
              742  GATC                
SapI          484  GAAGAGC              GCTCTTC(1/4)
              843  GAAGAGC             
Sau3AI        421  GATC                 ^GATC
              742  GATC                
TfiI          477  GATTC                G^A[AT]TC
              571  GATTC               
XmnI          120  GAAATACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              163  GAATGGGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BsaJI       
BssKI       BstF5I      BstNI       Cfr13I      DdeI        
EarI        EcoRII      FokI        Ksp632I     MboII       
MnlI        MseI        MvaI        MwoI        NlaIII      
NlaIV       RsaI        Sau96I      ScrFI       Tru9I       
Tsp509I     TspRI       
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