Restriction map for pF1KB9418
 (Circular) (Six-base) MAPSORT of: pF1KB9418.seq  Check: 8352  from: 1  to: 4970



pF1KB9418  4970 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:30  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    561     561 
   Size:       4970
 
AccI GT'mk_AC

Cuts at:   2008    2008 
   Size:       4970
 
Acc65I G'GTAC_C

Cuts at:   1986    1986 
   Size:       4970
 
AclI AA'CG_TT

Cuts at:   4810    4810 
   Size:       4970
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    335     512     572    1022    1674    1754    1850    2795    3227 
   Size:        177      60     450     652      80      96     945     432

Cuts at:   3227    3974    4242     335 
   Size:        747     268    1063

  Fragments arranged by size: 

                1063     945     747     652     450     432     268     177
                  96      80      60
 
AfeI AGC'GCT

Cuts at:    958     958 
   Size:       4970
 
AflIII A'CryG_T

Cuts at:    219    2322    3426    4403     219 
   Size:       2103    1104     977     786

  Fragments arranged by size: 

                2103    1104     977     786
 
AgeI A'CCGG_T

Cuts at:   3358    3358 
   Size:       4970
 
AleI CACnn'nnGTG

Cuts at:    523     523 
   Size:       4970
 
AlwNI CAG_nnn'CTG

Cuts at:   1489    2275    3842    1489 
   Size:        786    1567    2617

  Fragments arranged by size: 

                2617    1567     786
 
ApaI G_GGCC'C

Cuts at:   1609    1609 
   Size:       4970
 
ApaLI G'TGCA_C

Cuts at:   3740    3740 
   Size:       4970
 
ApoI r'AATT_y

Cuts at:    145    1152    1341    1974    4323    4579     145 
   Size:       1007     189     633    2349     256     536

  Fragments arranged by size: 

                2349    1007     633     536     256     189
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4970
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4970
 
AvaI C'yCGr_G

Cuts at:   1990    1990 
   Size:       4970
 
AvrII C'CTAG_G

Cuts at:   1405    1405 
   Size:       4970
 
BamHI G'GATC_C

Cuts at:   1014    1995    1014 
   Size:        981    3989
 
BanI G'GyrC_C

Cuts at:   1986    2400    2648    2683    1986 
   Size:        414     248      35    4273

  Fragments arranged by size: 

                4273     414     248      35
 
BanII G_rGCy'C

Cuts at:    278    1585    1609    1984    3014     278 
   Size:       1307      24     375    1030    2234

  Fragments arranged by size: 

                2234    1307    1030     375      24
 
BbeI G_GCGC'C

Cuts at:   2404    2652    2404 
   Size:        248    4722
 
BbsI GAAGACnn'nnnn_

Cuts at:   1028    1028 
   Size:       4970
 
BbvCI CC'TCA_GC

Cuts at:    413     413 
   Size:       4970
 
BciVI GTATCCnnnnn_n'

Cuts at:   2868    3629    4970    2868 
   Size:        761    1341    2868

  Fragments arranged by size: 

                2868    1341     761
 
BclI T'GATC_A

Cuts at:   2490    2490 
   Size:       4970
 
BglII A'GATC_T

Cuts at:    990    2485    4522     990 
   Size:       1495    2037    1438

  Fragments arranged by size: 

                2037    1495    1438
 
BlpI GC'TnA_GC

Cuts at:   2082    2082 
   Size:       4970
 
Bme1580I G_kGCm'C

Cuts at:   1609    2595    2688    3744    1609 
   Size:        986      93    1056    2835

  Fragments arranged by size: 

                2835    1056     986      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    508    2182    2263    2598    4742     508 
   Size:       1674      81     335    2144     736

  Fragments arranged by size: 

                2144    1674     736     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3337    3337 
   Size:       4970
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    745     745 
   Size:       4970
 
Bpu10I CC'TnA_GC

Cuts at:    413     413 
   Size:       4970
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1769    2146    3025    3517    3815    1769 
   Size:        377     879     492     298    2924

  Fragments arranged by size: 

                2924     879     492     377     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    886    4674     886 
   Size:       3788    1182
 
BsaAI yAC'GTr

Cuts at:   2323    2323 
   Size:       4970
 
BsaBI GATnn'nnATC

Cuts at:      6    1013    2484    4521       6 
   Size:       1007    1471    2037     455

  Fragments arranged by size: 

                2037    1471    1007     455
 
BsaHI Gr'CG_yC

Cuts at:   2401    2649    3351    4847    2401 
   Size:        248     702    1496    2524

  Fragments arranged by size: 

                2524    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1256    2159    2680    3358    3632    3779    4190    1256 
   Size:        903     521     678     274     147     411    2036

  Fragments arranged by size: 

                2036     903     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1218    1248    1704    1734    1218 
   Size:         30     456      30    4454

  Fragments arranged by size: 

                4454     456      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    412    1139    1577    1712     412 
   Size:        727     438     135    3670

  Fragments arranged by size: 

                3670     727     438     135
 
BseYI C'CCAG_C

Cuts at:    669     800    1247    2395    3153    3730    4226     669 
   Size:        131     447    1148     758     577     496    1413

  Fragments arranged by size: 

                1413    1148     758     577     496     447     131
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1240    1685    1240 
   Size:        445    4525
 
BsiEI CG_ry'CG

Cuts at:    104    1093    2512    2558    3766     104 
   Size:        989    1419      46    1208    1308

  Fragments arranged by size: 

                1419    1308    1208     989      46
 
BsiHKAI G_wGCw'C

Cuts at:    909    1984    2762    2952    3744     909 
   Size:       1075     778     190     792    2135

  Fragments arranged by size: 

                2135    1075     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    278     909    1585    1609    1984    2595    2688    2762    2952 
   Size:        631     676      24     375     611      93      74     190

Cuts at:   2952    3014    3744     278 
   Size:         62     730    1504

  Fragments arranged by size: 

                1504     730     676     631     611     375     190      93
                  74      62      24
 
BspEI T'CCGG_A

Cuts at:   1256    4190    1256 
   Size:       2934    2036
 
BspHI T'CATG_A

Cuts at:    375    4146     375 
   Size:       3771    1199
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    561    1474    1614    2020    2234    2536    2917     561 
   Size:        913     140     406     214     302     381    2614

  Fragments arranged by size: 

                2614     913     406     381     302     214     140
 
BsrBI CCG'CTC

Cuts at:     50    3262    3316    4800    4965      50 
   Size:       3212      54    1484     165      55

  Fragments arranged by size: 

                3212    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1644    1662    2882    1644 
   Size:         18    1220    3732

  Fragments arranged by size: 

                3732    1220      18
 
BsrFI r'CCGG_y

Cuts at:    253    1088    2968    3149    3358     253 
   Size:        835    1880     181     209    1865

  Fragments arranged by size: 

                1880    1865     835     209     181
 
BssSI C'ACGA_G

Cuts at:   3241    3599    3241 
   Size:        358    4612
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2347    2347 
   Size:       4970
 
BstBI TT'CG_AA

Cuts at:   3331    3331 
   Size:       4970
 
BstEII G'GTnAC_C

Cuts at:   1710    1710 
   Size:       4970
 
BstYI r'GATC_y

Cuts at:      1     990    1014    1041    1176    1473    1492    1995    2462 
   Size:        989      24      27     135     297      19     503     467

Cuts at:   2462    2485    2820    3066    4078    4164    4176    4522       1 
   Size:         23     335     246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     989     503     467     449     346     335     297
                 246     135      86      27      24      23      19      12
            
 
BtgI C'CryG_G

Cuts at:    107     896     107 
   Size:        789    4181
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2920    3101    3264    2920 
   Size:        181     163    4626

  Fragments arranged by size: 

                4626     181     163
 
BtsI GCAGTG_nn'

Cuts at:    754     848    2213     754 
   Size:         94    1365    3511

  Fragments arranged by size: 

                3511    1365      94
 
DraI TTT'AAA

Cuts at:    822     975    1969     822 
   Size:        153     994    3823

  Fragments arranged by size: 

                3823     994     153
 
DrdI GACnn_nn'nnGTC

Cuts at:   2676    3534    2676 
   Size:        858    4112
 
EaeI y'GGCC_r

Cuts at:   2555    2729    3120    3147    4492    2555 
   Size:        174     391      27    1345    3033

  Fragments arranged by size: 

                3033    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   2555    2555 
   Size:       4970
 
EarI CTCTTCn'nnn_

Cuts at:    266     897    1258    2993    3203     266 
   Size:        631     361    1735     210    2033

  Fragments arranged by size: 

                2033    1735     631     361     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3178    3488    3634    4780    3178 
   Size:        310     146    1146    3368

  Fragments arranged by size: 

                3368    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1982    1982 
   Size:       4970
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    335     512     572     745    1022    1674    1754    1850    2795 
   Size:        177      60     173     277     652      80      96     945

Cuts at:   2795    3227    3974    4242     335 
   Size:        432     747     268    1063

  Fragments arranged by size: 

                1063     945     747     652     432     277     268     177
                 173      96      80      60
 
EcoO109I rG'GnC_Cy

Cuts at:   1606    1626    2109    1606 
   Size:         20     483    4467

  Fragments arranged by size: 

                4467     483      20
 
EcoRI G'AATT_C

Cuts at:   1152    1974    1152 
   Size:        822    4148
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2989    2989 
   Size:       4970
 
FspI TGC'GCA

Cuts at:   2751    2751 
   Size:       4970
 
HaeII r_GCGC'y

Cuts at:    960    2404    2652    3674    4653     960 
   Size:       1444     248    1022     979    1277

  Fragments arranged by size: 

                1444    1277    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1704    1736    2812    2842    2844    2874    1704 
   Size:         32    1076      30       2      30    3800

  Fragments arranged by size: 

                3800    1076      32      30      30       2
 
HincII GTy'rAC

Cuts at:   2009    2009 
   Size:       4970
 
HindIII A'AGCT_T

Cuts at:    365     365 
   Size:       4970
 
KasI G'GCGC_C

Cuts at:   2400    2648    2400 
   Size:        248    4722
 
KpnI G_GTAC'C

Cuts at:   1990    1990 
   Size:       4970
 
MfeI C'AATT_G

Cuts at:    392    1595     392 
   Size:       1203    3767
 
MluI A'CGCG_T

Cuts at:   4403    4403 
   Size:       4970
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    147    1402    3641    3825     147 
   Size:       1255    2239     184    1292

  Fragments arranged by size: 

                2239    1292    1255     184
 
MscI TGG'CCA

Cuts at:   2731    2731 
   Size:       4970
 
MslI CAynn'nnrTG

Cuts at:    523    2959    3086     523 
   Size:       2436     127    2407

  Fragments arranged by size: 

                2436    2407     127
 
MspA1I CmG'CkG

Cuts at:   1737    2081    2395    2755    3768    4013    1737 
   Size:        344     314     360    1013     245    2694

  Fragments arranged by size: 

                2694    1013     360     344     314     245
 
NaeI GCC'GGC

Cuts at:   3151    3151 
   Size:       4970
 
NarI GG'CG_CC

Cuts at:   2401    2649    2401 
   Size:        248    4722
 
NcoI C'CATG_G

Cuts at:    107     896     107 
   Size:        789    4181
 
NgoMIV G'CCGG_C

Cuts at:   3149    3149 
   Size:       4970
 
NspI r_CATG'y

Cuts at:    223    2023    2523    3430     223 
   Size:       1800     500     907    1763

  Fragments arranged by size: 

                1800    1763     907     500
 
PciI A'CATG_T

Cuts at:    219    3426     219 
   Size:       3207    1763
 
PflMI CCAn_nnn'nTGG

Cuts at:    854     854 
   Size:       4970
 
PfoI T'CCnGG_A

Cuts at:    761     761 
   Size:       4970
 
PmeI GTTT'AAAC

Cuts at:   1969    1969 
   Size:       4970
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4109    4141    4109 
   Size:         32    4938
 
PpuMI rG'GwC_Cy

Cuts at:   1626    1626 
   Size:       4970
 
PspOMI G'GGCC_C

Cuts at:   1605    1605 
   Size:       4970
 
PstI C_TGCA'G

Cuts at:   1568    2017    2702    1568 
   Size:        449     685    3836

  Fragments arranged by size: 

                3836     685     449
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4970
 
PvuII CAG'CTG

Cuts at:   1737    2395    2755    1737 
   Size:        658     360    3952

  Fragments arranged by size: 

                3952     658     360
 
RsrII CG'GwC_CG

Cuts at:   3165    3165 
   Size:       4970
 
SacI G_AGCT'C

Cuts at:   1984    1984 
   Size:       4970
 
SalI G'TCGA_C

Cuts at:   2007    2007 
   Size:       4970
 
SapI GCTCTTCn'nnn_

Cuts at:    266     897    1258    2993    3203     266 
   Size:        631     361    1735     210    2033

  Fragments arranged by size: 

                2033    1735     631     361     210
 
SbfI CC_TGCA'GG

Cuts at:   2017    2017 
   Size:       4970
 
ScaI AGT'ACT

Cuts at:   1544    4487    1544 
   Size:       2943    2027
 
SfcI C'TryA_G

Cuts at:     33    1133    1564    2013    2698    3691    3882      33 
   Size:       1100     431     449     685     993     191    1121

  Fragments arranged by size: 

                1121    1100     993     685     449     431     191
 
SfoI GGC'GCC

Cuts at:   2402    2650    2402 
   Size:        248    4722
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4970
 
SmaI CCC'GGG

Cuts at:   1992    1992 
   Size:       4970
 
SmlI C'TyrA_G

Cuts at:   1784    2125    3040    3532    3794    1784 
   Size:        341     915     492     262    2960

  Fragments arranged by size: 

                2960     915     492     341     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4970
 
SphI G_CATG'C

Cuts at:   2023    2523    2023 
   Size:        500    4470
 
StyI C'CwwG_G

Cuts at:    107     896    1405    2104    2458     107 
   Size:        789     509     699     354    2619

  Fragments arranged by size: 

                2619     789     699     509     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    535    1763    2553    2686    3145    3354     535 
   Size:       1228     790     133     459     209    2151

  Fragments arranged by size: 

                2151    1228     790     459     209     133
 
TatI w'GTAC_w

Cuts at:   1272    1281    1542    4485    1272 
   Size:          9     261    2943    1757

  Fragments arranged by size: 

                2943    1757     261       9
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1218    1250    4487    4519    1218 
   Size:         32    3237      32    1669

  Fragments arranged by size: 

                3237    1669      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2767    2767 
   Size:       4970
 
XbaI T'CTAG_A

Cuts at:   2001    2001 
   Size:       4970
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1179    2465    1179 
   Size:       1286    3684
 
XmaI C'CCGG_G

Cuts at:   1990    1990 
   Size:       4970
 
XmnI GAAnn'nnTTC

Cuts at:    221     221 
   Size:       4970
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AleI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    AvrII    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI
     BclI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstYI
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI   PspOMI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII    AflII     AhdI     AlfI     AloI     AscI     BaeI     BcgI
    BfrBI     BglI    BmgBI     BmtI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI
    EcoRV     FseI    FspAI     HpaI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PmlI    PshAI     PsiI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     ZraI
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