Restriction map for pF1KB9351
##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

Acc65I      AclI        AocI        ApaI        AscI        
Asp718I     AvrII       BbeI        BbrPI       BciVI       
BclI        BglI        BlnI        BsaAI       BsaBI       
BsiEI       BsiWI       BsmBI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CpoI        EagI        EclXI       Eco52I      Eco81I      
EcoO65I     EcoRV       FbaI        FseI        HgaI        
HpaI        KasI        KpnI        MamI        MluI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RsrII       SalI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII        2936  GACGTC               GACGT^C
AccII        4839  CGCG                 CG^CG
AccIII        812  TCCGGA               T^CCGGA
AcyI         2936  GACGTC               G[GA]^CG[CT]C
AflII        2317  CTTAAG               C^TTAAG
AgeI         4796  ACCGGT               A^CCGGT
Aor51HI       574  AGCGCT               AGC^GCT
AseI         3075  ATTAAT               AT^TAAT
AsnI         3075  ATTAAT               AT^TAAT
AspI         1557  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         5189  CCCGGG               C^[CT]CG[GA]G
AviII        3129  TGCGCA               TGC^GCA
BamHI         175  GGATCC               G^GATCC
BbvCI        5613  CCTCAGC              CCTCAGC(-5/-2)
BfrI         2317  CTTAAG               C^TTAAG
BsaHI        2936  GACGTC               G[GA]^CG[CT]C
BseAI         812  TCCGGA               T^CCGGA
BsoBI        5189  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     2636  TGTACA               T^GTACA
BspDI        3373  ATCGAT               AT^CGAT
BspEI         812  TCCGGA               T^CCGGA
BspLU11I     4380  ACATGT               A^CATGT
BsrFI        4796  ACCGGT               [GA]^CCGG[CT]
BsrGI        2636  TGTACA               T^GTACA
BstUI        4839  CGCG                 CG^CG
Cfr10I       4796  ACCGGT               [GA]^CCGG[CT]
ClaI         3373  ATCGAT               AT^CGAT
DrdI         4488  GACTTATTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      574  AGCGCT               AGC^GCT
EcoRI        5527  GAATTC               G^AATTC
FspI         3129  TGCGCA               TGC^GCA
Hin1I        2936  GACGTC               G[GA]^CG[CT]C
KspI         4838  CCGCGG               CCGC^GG
MroI          812  TCCGGA               T^CCGGA
MvnI         4839  CGCG                 CG^CG
NdeI          450  CATATG               CA^TATG
PflFI        1557  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        4796  ACCGGT               A^CCGGT
PshBI        3075  ATTAAT               AT^TAAT
SacI         3166  GAGCTC               GAGCT^C
SacII        4838  CCGCGG               CCGC^GG
SexAI        1487  ACCTGGT              A^CC[AT]GGT
SmaI         5189  CCCGGG               CCC^GGG
SspBI        2636  TGTACA               T^GTACA
Tth111I      1557  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        5189  CCCGGG               C^CCGGG
XmaI         5189  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AflIII       1858  ACGTGT               A^C[GA][CT]GT
             4380  ACATGT              
AhdI         2612  GACTTGTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3868  GACAGAGAGTC         
AspEI        2612  GACTTGTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3868  GACAGAGAGTC         
BbsI         5393  GAAGAC               GAAGAC(2/6)
              147  GTCTTC              
BcgI           73  CGAGACCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4608  GCAGCTTTGTCG        
BglII        2176  AGATCT               A^GATCT
             4141  AGATCT              
BlpI         2021  GCTTAGC              GC^T[ACGT]AGC
             5643  GCTGAGC             
Bpu1102I     2021  GCTTAGC              GC^T[ACGT]AGC
             5643  GCTGAGC             
BpuAI        5393  GAAGAC               GAAGAC(2/6)
              147  GTCTTC              
BsaI           74  GAGACC               GGTCTC(1/5)
             5344  GAGACC              
BsaWI         812  TCCGGA               [AT]^CCGG[AT]
             4796  ACCGGT              
BspHI        1698  TCATGA               T^CATGA
             1991  TCATGA              
BspMI        2277  ACCTGC               ACCTGC(4/8)
             5696  ACCTGC              
BsrBI        4745  CCGCTC               CCGCTC(-3/-3)
             5482  GAGCGG              
CelII        2021  GCTTAGC              GC^T[ACGT]AGC
             5643  GCTGAGC             
DraI          846  TTTAAA               TTT^AAA
             4986  TTTAAA              
Eam1105I     2612  GACTTGTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3868  GACAGAGAGTC         
HindIII      2201  AAGCTT               A^AGCTT
             5727  AAGCTT              
PpuMI        1947  AGGACCC              [GA]G^G[AT]CC[CT]
             4521  AGGTCCT             
PvuII         121  CAGCTG               CAG^CTG
             5641  CAGCTG              
RcaI         1698  TCATGA               T^CATGA
             1991  TCATGA              
SmlI         2317  CTTAAG               C^T[CT][GA]AG
             2622  CTTGAG              
SspI         2123  AATATT               AAT^ATT
             5255  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
Alw44I      AlwI        AlwNI       ApaLI       ApoI        
Asp700I     AspHI       AvaII       BalI        BanI        
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    BsrDI       BsrI        BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     EcoT22I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MfeI        MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MunI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NsiI        NspI        PflMI       
PleI        PstI        RsaI        SapI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SphI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII       XmnI        
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