Restriction map for pF1KB9022
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AocI        Aor51HI     
AscI        AseI        AsnI        Asp718I     AviII       
AvrII       BbrPI       BbvCI       BcgI        BclI        
BfrI        BglII       BlnI        BsaAI       BsaWI       
BseAI       BsiWI       BspDI       BspEI       BspHI       
BspLU11I    BssSI       Bst1107I    BstBI       BstZ17I     
Bsu36I      ClaI        CpoI        Eco47III    Eco81I      
EcoT22I     FbaI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        MfeI        MluI        
MroI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SbfI        ScaI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspI        SwaI        
XhoI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AhdI         4206  GACAGTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       4403  GTGCAC               G^TGCAC
ApaLI        4403  GTGCAC               G^TGCAC
Asp700I      3351  GAAGGGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        4206  GACAGTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          176  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         3852  GGCGCC               GGCGC^C
BlpI         2335  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2335  GCTCAGC              GC^T[ACGT]AGC
BsgI         2231  GTGCAG               GTGCAG(16/14)
BsiEI         594  CGGCCG               CG[GA][CT]^CG
Bsp1407I     1453  TGTACA               T^GTACA
BsrDI        4973  GCAATG               GCAATG(2/0)
BsrGI        1453  TGTACA               T^GTACA
BssHII         15  GCGCGC               G^CGCGC
BstAPI       1072  GCAAAAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        2335  GCTCAGC              GC^T[ACGT]AGC
DraIII       3836  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          594  CGGCCG               C^GGCCG
Eam1105I     4206  GACAGTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         594  CGGCCG               C^GGCCG
Eco52I        594  CGGCCG               C^GGCCG
EcoNI         163  CCTGAGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         353  GAATTC               G^AATTC
EcoRV         542  GATATC               GAT^ATC
KasI         3852  GGCGCC               G^GCGCC
MslI         1986  CACAGATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI         3852  GGCGCC               GG^CGCC
PflFI         176  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          247  CTGCAG               CTGCA^G
SfcI          247  CTGCAG               C^T[GA][CT]AG
SfoI         3852  GGCGCC               GGC^GCC
SphI         2795  GCATGC               GCATG^C
SspBI        1453  TGTACA               T^GTACA
Tth111I       176  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         4938  TCTAGA               T^CTAGA
XmnI         3351  GAAGGGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcsI          353  GAATTC               [GA]^AATT[CT]
             3291  AAATTT              
ApoI          353  GAATTC               [GA]^AATT[CT]
             3291  AAATTT              
BbsI         3302  GAAGAC               GAAGAC(2/6)
             1481  GTCTTC              
BfaI         1069  CTAG                 C^TAG
             4939  CTAG                
BpuAI        3302  GAAGAC               GAAGAC(2/6)
             1481  GTCTTC              
BsaBI         973  GATGGCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1183  GATGGAGATC          
BsrBI        1672  GAGCGG               CCGCTC(-3/-3)
             3322  GAGCGG              
BstXI        1417  CCAAAGGGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4837  CCAGCTGGGTGG        
DraI         2716  TTTAAA               TTT^AAA
             2896  TTTAAA              
DrdI          614  GACAGATGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3112  GACATCGGAGTC        
FseI           24  GGCCGGCC             GGCCGG^CC
             5082  GGCCGGCC            
HaeII        2623  GGCGCT               [GA]GCGC^[CT]
             3852  GGCGCC              
MaeI         1069  CTAG                 C^TAG
             4939  CTAG                
MamI          973  GATGGCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1183  GATGGAGATC          
NspI          606  GCATGT               [GA]CATG^[CT]
             2795  GCATGC              
PvuII        4838  CAGCTG               CAG^CTG
             4932  CAGCTG              
SapI         1370  GAAGAGC              GCTCTTC(1/4)
             3913  GCTCTTC             


#########################################
# 3 OR MORE CUT ENZYMES (  123 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BalI        BamHI       BanI        BanII       
BbvI        BciVI       BcnI        BglI        BmyI        
BpmI        BsaHI       BsaI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrFI       BsrI        BssKI       BstEII      BstF5I      
BstNI       BstPI       BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     KspI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       PflMI       PleI        
PpuMI       PshAI       RsaI        SacI        SacII       
Sau3AI      Sau96I      ScrFI       SexAI       SfaNI       
SfiI        SmaI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI        
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