Restriction map for pF1KB7018
##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

Acc65I      AgeI        AocI        Aor51HI     AscI        
Asp700I     Asp718I     AvaI        AviII       AvrII       
BalI        BamHI       BbvCI       BcgI        BciVI       
BglI        BlnI        BsaI        BsiWI       BsoBI       
Bsp1407I    BspDI       BspLU11I    BsrDI       BsrGI       
BssHII      BstBI       BstEII      BstPI       BstXI       
Bsu36I      ClaI        CpoI        DraIII      EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FseI        FspI        
HpaI        KpnI        KspI        MfeI        MluI        
MluNI       MscI        MunI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PaeR7I      PinAI       PmeI        
PpuMI       PshAI       PvuI        PvuII       RsrII       
SacI        SacII       SalI        SbfI        SfiI        
SfuI        SgrAI       SmaI        SpeI        Sse8387I    
SspBI       StuI        SwaI        XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccIII       2648  TCCGGA               T^CCGGA
AclI          819  AACGTT               AA^CGTT
AflII         306  CTTAAG               C^TTAAG
AhdI         2539  GACGTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         1978  GGGCCC               GGGCC^C
AseI          937  ATTAAT               AT^TAAT
AsnI          937  ATTAAT               AT^TAAT
AspEI        2539  GACGTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2908  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI           16  GGCGCC               GGCGC^C
BbrPI         285  CACGTG               CAC^GTG
BcnI          181  CCGGG                CC^[GC]GG
BfrI          306  CTTAAG               C^TTAAG
BglII        2206  AGATCT               A^GATCT
BsaBI        2710  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2648  TCCGGA               [AT]^CCGG[AT]
BseAI        2648  TCCGGA               T^CCGGA
BsiEI        2609  CGGTCG               CG[GA][CT]^CG
BsmBI         101  CGTCTC               CGTCTC(1/5)
BsmFI        2182  GTCCC                GGGAC(10/14)
BspEI        2648  TCCGGA               T^CCGGA
BspHI         509  TCATGA               T^CATGA
BspMI        2673  ACCTGC               ACCTGC(4/8)
BsrBI         363  CCGCTC               CCGCTC(-3/-3)
BsrI         1366  CCAGT                ACTGG(1/-1)
BssSI        1261  CTCGTG               CACGAG(-5/-1)
Bst1107I     2521  GTATAC               GTA^TAC
BstZ17I      2521  GTATAC               GTA^TAC
DraI         1522  TTTAAA               TTT^AAA
DraII        1977  AGGGCCC              [GA]G^G[ACGT]CC[CT]
DsaI         2834  CCGTGG               C^C[GA][CT]GG
EaeI         1835  CGGCCA               [CT]^GGCC[GA]
Eam1105I     2539  GACGTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I     1977  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         682  GAATTC               G^AATTC
HgaI           56  GCGTC                GACGC(5/10)
HindIII       346  AAGCTT               A^AGCTT
KasI           16  GGCGCC               G^GCGCC
MamI         2710  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2648  TCCGGA               T^CCGGA
MspA1I        402  CCGCTG               C[AC]G^C[GT]G
NarI           16  GGCGCC               GG^CGCC
NciI          181  CCGGG                CC^[GC]GG
NspI         1271  GCATGC               [GA]CATG^[CT]
PacI         1819  TTAATTAA             TTAAT^TAA
PflFI        2908  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         285  CACGTG               CAC^GTG
PmlI          285  CACGTG               CAC^GTG
PshBI         937  ATTAAT               AT^TAAT
Psp1406I      819  AACGTT               AA^CGTT
RcaI          509  TCATGA               T^CATGA
SapI           68  GCTCTTC              GCTCTTC(1/4)
ScaI         2137  AGTACT               AGT^ACT
SexAI        2309  ACCAGGT              A^CC[AT]GGT
SfoI           16  GGCGCC               GGC^GCC
SnaBI        1117  TACGTA               TAC^GTA
SphI         1271  GCATGC               GCATG^C
Tth111I      2908  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AatII        1192  GACGTC               GACGT^C
             2539  GACGTC              
AccI         2521  GTATAC               GT^[AC][GT]AC
             2839  GTAGAC              
AflIII        284  ACACGT               A^C[GA][CT]GT
             2525  ACACGT              
BanII        1303  GAGCCC               G[GA]GC[CT]^C
             1978  GGGCCC              
BlpI         2295  GCTGAGC              GC^T[ACGT]AGC
             2655  GCTCAGC             
Bpu1102I     2295  GCTGAGC              GC^T[ACGT]AGC
             2655  GCTCAGC             
BsaAI         285  CACGTG               [CT]AC^GT[GA]
             1117  TACGTA              
BseRI        2883  CTCCTC               GAGGAG(10/8)
             1669  GAGGAG              
BsrFI         274  ACCGGC               [GA]^CCGG[CT]
             2607  GCCGGT              
BstAPI       1589  GCATGATGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2080  GCAGAAGCTGC         
CelII        2295  GCTGAGC              GC^T[ACGT]AGC
             2655  GCTCAGC             
Cfr10I        274  ACCGGC               [GA]^CCGG[CT]
             2607  GCCGGT              
DrdI          133  GACTTAATAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              391  GACACGCTGGTC        
EarI           69  CTCTTC               CTCTTC(1/4)
              464  CTCTTC              
FauI         2248  CCCGC                CCCGC(4/6)
              502  GCGGG               
HaeII           7  GGCGCT               [GA]GCGC^[CT]
               16  GGCGCC              
HincII        358  GTTGAC               GT[CT]^[GA]AC
              822  GTTGAC              
HindII        358  GTTGAC               GT[CT]^[GA]AC
              822  GTTGAC              
HphI          793  GGTGA                GGTGA(8/7)
             2014  GGTGA               
Ksp632I        69  CTCTTC               CTCTTC(1/4)
              464  CTCTTC              
PflMI        1278  CCAATGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1399  CCACCGTTTGG         
PstI         2127  CTGCAG               CTGCA^G
             2811  CTGCAG              
TaqI         2475  TCGA                 T^CGA
             2612  TCGA                
TthHB8I      2475  TCGA                 T^CGA
             2612  TCGA                
Van91I       1278  CCAATGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1399  CCACCGTTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        Alw44I      AlwI        AlwNI       ApaLI       
ApoI        AspHI       AvaII       BanI        BbsI        
BbvI        BclI        BfaI        BmyI        BpmI        
BpuAI       BsaHI       BsaJI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmI        Bsp1286I    
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EcoRII      EcoT14I     FbaI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HinfI       HpaII       ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspI        MvaI        MvnI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XhoII       
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