Restriction map for pF1KB7018
 (Circular) (Six-base) MAPSORT of: pF1KB7018.seq  Check: 232  from: 1  to: 6029



pF1KB7018  6029 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2790    2790 
   Size:       6029
 
AatII G_ACGT'C

Cuts at:   1304    2651    1304 
   Size:       1347    4682
 
AccI GT'mk_AC

Cuts at:   2630    2948    3067    2630 
   Size:        318     119    5592

  Fragments arranged by size: 

                5592     318     119
 
Acc65I G'GTAC_C

Cuts at:   3045    3045 
   Size:       6029
 
AclI AA'CG_TT

Cuts at:    928    5869     928 
   Size:       4941    1088
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   3854    4286    4639    5301    3854 
   Size:        432     353     662    4582

  Fragments arranged by size: 

                4582     662     432     353
 
AflII C'TTAA_G

Cuts at:    414     414 
   Size:       6029
 
AflIII A'CryG_T

Cuts at:    392    2633    3381    5181    5462     392 
   Size:       2241     748    1800     281     959

  Fragments arranged by size: 

                2241    1800     959     748     281
 
AhdI GACnn_n'nnGTC

Cuts at:   2652    2652 
   Size:       6029
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    706     706 
   Size:       6029
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2006    2038    2006 
   Size:         32    5997
 
AlwNI CAG_nnn'CTG

Cuts at:   1444    2194    3003    3334    4772    1444 
   Size:        750     809     331    1438    2701

  Fragments arranged by size: 

                2701    1438     809     750     331
 
ApaI G_GGCC'C

Cuts at:   2090    2090 
   Size:       6029
 
ApaLI G'TGCA_C

Cuts at:    396     804    3010    4867     396 
   Size:        408    2206    1857    1558

  Fragments arranged by size: 

                2206    1857    1558     408
 
ApoI r'AATT_y

Cuts at:    703     790    2133    3033    5382    5638     703 
   Size:         87    1343     900    2349     256    1094

  Fragments arranged by size: 

                2349    1343    1094     900     256      87
 
AseI AT'TA_AT

Cuts at:     20    1046      20 
   Size:       1026    5003
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6029
 
AvaI C'yCGr_G

Cuts at:   3049    3049 
   Size:       6029
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   1215    1248    1215 
   Size:         33    5996
 
BamHI G'GATC_C

Cuts at:   3054    3054 
   Size:       6029
 
BanI G'GyrC_C

Cuts at:    124     557    1258    2604    3045    3459    3707    3742     124 
   Size:        433     701    1346     441     414     248      35    2411

  Fragments arranged by size: 

                2411    1346     701     441     433     414     248      35
            
 
BanII G_rGCy'C

Cuts at:   1415    2090    3043    4073    1415 
   Size:        675     953    1030    3371

  Fragments arranged by size: 

                3371    1030     953     675
 
BbeI G_GCGC'C

Cuts at:    128    3463    3711     128 
   Size:       3335     248    2446

  Fragments arranged by size: 

                3335    2446     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    497    2222    2402    2812     497 
   Size:       1725     180     410    3714

  Fragments arranged by size: 

                3714    1725     410     180
 
BciVI GTATCCnnnnn_n'

Cuts at:   3927    4983    6029    3927 
   Size:       1056    1046    3927

  Fragments arranged by size: 

                3927    1056    1046
 
BclI T'GATC_A

Cuts at:    620    1830    2741    3549     620 
   Size:       1210     911     808    3100

  Fragments arranged by size: 

                3100    1210     911     808
 
BglII A'GATC_T

Cuts at:   2314    3544    5581    2314 
   Size:       1230    2037    2762

  Fragments arranged by size: 

                2762    2037    1230
 
BlpI GC'TnA_GC

Cuts at:   2404    2764    3141    2404 
   Size:        360     377    5292

  Fragments arranged by size: 

                5292     377     360
 
Bme1580I G_kGCm'C

Cuts at:    400     808    1983    2090    3014    3654    3747    4871     400 
   Size:        408    1175     107     924     640      93    1124    1558

  Fragments arranged by size: 

                1558    1175    1124     924     640     408     107      93
            
 
BmgBI CAC'GTC

Cuts at:    803     803 
   Size:       6029
 
BmrI ACTGGGnnnn_n'

Cuts at:   3241    3322    3657    5801    3241 
   Size:         81     335    2144    3469

  Fragments arranged by size: 

                3469    2144     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    134    2700    4396     134 
   Size:       2566    1696    1767

  Fragments arranged by size: 

                2566    1767    1696
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    983    1907    2294     983 
   Size:        924     387    4718

  Fragments arranged by size: 

                4718     924     387
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    535     606    1954    3205    4084    4798    5096     535 
   Size:         71    1348    1251     879     714     298    1468

  Fragments arranged by size: 

                1468    1348    1251     879     714     298      71
 
BsaI GGTCTCn'nnnn_

Cuts at:   5733    5733 
   Size:       6029
 
BsaAI yAC'GTr

Cuts at:    395    1227    3382     395 
   Size:        832    2155    3042

  Fragments arranged by size: 

                3042    2155     832
 
BsaBI GATnn'nnATC

Cuts at:      6    2822    3543    5580       6 
   Size:       2816     721    2037     455

  Fragments arranged by size: 

                2816    2037     721     455
 
BsaHI Gr'CG_yC

Cuts at:    125    1301    2648    3460    3708    4410    5906     125 
   Size:       1176    1347     812     248     702    1496     248

  Fragments arranged by size: 

                1496    1347    1176     812     702     248     248
 
BsaWI w'CCGG_w

Cuts at:   2756    3218    3739    4417    4828    4975    5249    2756 
   Size:        462     521     678     411     147     274    3536

  Fragments arranged by size: 

                3536     678     521     462     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    670     700    1082    1112     670 
   Size:         30     382      30    5587

  Fragments arranged by size: 

                5587     382      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1792    2982    1792 
   Size:       1190    4839
 
BseYI C'CCAG_C

Cuts at:   2813    2996    3454    4212    4877    5285    2813 
   Size:        183     458     758     665     408    3557

  Fragments arranged by size: 

                3557     758     665     458     408     183
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1504    2058    2180    1504 
   Size:        554     122    5353

  Fragments arranged by size: 

                5353     554     122
 
BsiEI CG_ry'CG

Cuts at:    104    2720    3571    3617    4847     104 
   Size:       2616     851      46    1230    1286

  Fragments arranged by size: 

                2616    1286    1230     851      46
 
BsiHKAI G_wGCw'C

Cuts at:    400     808    2890    3014    3043    3821    4011    4871     400 
   Size:        408    2082     124      29     778     190     860    1558

  Fragments arranged by size: 

                2082    1558     860     778     408     190     124      29
            
 
BsmI GAATG_Cn'

Cuts at:   1123    1552    2217    2717    1123 
   Size:        429     665     500    4435

  Fragments arranged by size: 

                4435     665     500     429
 
BsmBI CGTCTCn'nnnn_

Cuts at:    215     215 
   Size:       6029
 
Bsp1286I G_dGCh'C

Cuts at:    400     808    1415    1983    2090    2890    3014    3043    3654 
   Size:        408     607     568     107     800     124      29     611

Cuts at:   3654    3747    3821    4011    4073    4871     400 
   Size:         93      74     190      62     798    1558

  Fragments arranged by size: 

                1558     800     798     611     607     568     408     190
                 124     107      93      74      62      29
 
BspEI T'CCGG_A

Cuts at:   2756    2756 
   Size:       6029
 
BspHI T'CATG_A

Cuts at:    617    4461     617 
   Size:       3844    2185
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2790    3079    3293    3595    3976    2790 
   Size:        289     214     302     381    4843

  Fragments arranged by size: 

                4843     381     302     289     214
 
BsrBI CCG'CTC

Cuts at:     50     473    4321    4375    5859    6024      50 
   Size:        423    3848      54    1484     165      55

  Fragments arranged by size: 

                3848    1484     423     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   3941    3941 
   Size:       6029
 
BsrFI r'CCGG_y

Cuts at:    382    2715    4027    4208     382 
   Size:       2333    1312     181    2203

  Fragments arranged by size: 

                2333    2203    1312     181
 
BssSI C'ACGA_G

Cuts at:   1369    4300    5008    1369 
   Size:       2931     708    2390

  Fragments arranged by size: 

                2931    2390     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1703    2194    3406    1703 
   Size:        491    1212    4326

  Fragments arranged by size: 

                4326    1212     491
 
BstBI TT'CG_AA

Cuts at:   4390    4390 
   Size:       6029
 
BstYI r'GATC_y

Cuts at:      1     314     444    1151    2314    2823    3054    3521    3544 
   Size:        313     130     707    1163     509     231     467      23

Cuts at:   3544    3879    4125    4431    4443    4529    5581       1 
   Size:        335     246     306      12      86    1052     449

  Fragments arranged by size: 

                1163    1052     707     509     467     449     335     313
                 306     246     231     130      86      23      12
 
BstZ17I GTA'TAC

Cuts at:   2631    2631 
   Size:       6029
 
BtgI C'CryG_G

Cuts at:   2942    2942 
   Size:       6029
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3979    4160    4323    3979 
   Size:        181     163    5685

  Fragments arranged by size: 

                5685     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1153    3272    1153 
   Size:       2119    3910
 
DraI TTT'AAA

Cuts at:   1632    3028    1632 
   Size:       1396    4633
 
DrdI GACnn_nn'nnGTC

Cuts at:    247     505    3735    5079     247 
   Size:        258    3230    1344    1197

  Fragments arranged by size: 

                3230    1344    1197     258
 
EaeI y'GGCC_r

Cuts at:   1943    3614    3788    4179    4206    5551    1943 
   Size:       1671     174     391      27    1345    2421

  Fragments arranged by size: 

                2421    1671    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   3614    3614 
   Size:       6029
 
EarI CTCTTCn'nnn_

Cuts at:    183     578    4052    4262     183 
   Size:        395    3474     210    1950

  Fragments arranged by size: 

                3474    1950     395     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   4237    4979    5125    5839    4237 
   Size:        742     146     714    4427

  Fragments arranged by size: 

                4427     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   3041    3041 
   Size:       6029
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    983    1907    2294    3854    4286    4639    5301     983 
   Size:        924     387    1560     432     353     662    1711

  Fragments arranged by size: 

                1711    1560     924     662     432     387     353
 
EcoO109I rG'GnC_Cy

Cuts at:   2086    3168    2086 
   Size:       1082    4947
 
EcoRI G'AATT_C

Cuts at:    790    3033     790 
   Size:       2243    3786
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2215    2769    4048    2215 
   Size:        554    1279    4196

  Fragments arranged by size: 

                4196    1279     554
 
FspI TGC'GCA

Cuts at:   3810    3810 
   Size:       6029
 
HaeII r_GCGC'y

Cuts at:    119     128    3463    3711    4941    5712     119 
   Size:          9    3335     248    1230     771     436

  Fragments arranged by size: 

                3335    1230     771     436     248       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    670     702    2638    2670    2877    2909    3871    3901    3903 
   Size:         32    1936      32     207      32     962      30       2

Cuts at:   3903    3933     670 
   Size:         30    2766

  Fragments arranged by size: 

                2766    1936     962     207      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:    468     932    3068     468 
   Size:        464    2136    3429

  Fragments arranged by size: 

                3429    2136     464
 
HindIII A'AGCT_T

Cuts at:    454     454 
   Size:       6029
 
KasI G'GCGC_C

Cuts at:    124    3459    3707     124 
   Size:       3335     248    2446

  Fragments arranged by size: 

                3335    2446     248
 
KpnI G_GTAC'C

Cuts at:   3049    3049 
   Size:       6029
 
MluI A'CGCG_T

Cuts at:   5462    5462 
   Size:       6029
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    677    2485    2930    4788    4972     677 
   Size:       1808     445    1858     184    1734

  Fragments arranged by size: 

                1858    1808    1734     445     184
 
MscI TGG'CCA

Cuts at:   3790    3790 
   Size:       6029
 
MslI CAynn'nnrTG

Cuts at:    430     899    1711    2263    3017    4018    4145     430 
   Size:        469     812     552     754    1001     127    2314

  Fragments arranged by size: 

                2314    1001     812     754     552     469     127
 
MspA1I CmG'CkG

Cuts at:    512    3140    3454    3814    4598    4843     512 
   Size:       2628     314     360     784     245    1698

  Fragments arranged by size: 

                2628    1698     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   4210    4210 
   Size:       6029
 
NarI GG'CG_CC

Cuts at:    125    3460    3708     125 
   Size:       3335     248    2446

  Fragments arranged by size: 

                3335    2446     248
 
NgoMIV G'CCGG_C

Cuts at:   4208    4208 
   Size:       6029
 
NspI r_CATG'y

Cuts at:   1383    3082    3582    5185    1383 
   Size:       1699     500    1603    2227

  Fragments arranged by size: 

                2227    1699    1603     500
 
PacI TTA_AT'TAA

Cuts at:   1931    1931 
   Size:       6029
 
PasI CC'CwG_GG

Cuts at:   1878    1878 
   Size:       6029
 
PciI A'CATG_T

Cuts at:   5181    5181 
   Size:       6029
 
PflMI CCAn_nnn'nTGG

Cuts at:   1392    1513    1392 
   Size:        121    5908
 
PfoI T'CCnGG_A

Cuts at:    228     317    1437    2706     228 
   Size:         89    1120    1269    3551

  Fragments arranged by size: 

                3551    1269    1120      89
 
PmeI GTTT'AAAC

Cuts at:   3028    3028 
   Size:       6029
 
PmlI CAC'GTG

Cuts at:    395     395 
   Size:       6029
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4475    4507    4475 
   Size:         32    5997
 
PsiI TTA'TAA

Cuts at:   2204    2204 
   Size:       6029
 
PspOMI G'GGCC_C

Cuts at:   2086    2086 
   Size:       6029
 
PstI C_TGCA'G

Cuts at:   2239    2923    3076    3761    2239 
   Size:        684     153     685    4507

  Fragments arranged by size: 

                4507     685     684     153
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6029
 
PvuII CAG'CTG

Cuts at:   3454    3814    3454 
   Size:        360    5669
 
RsrII CG'GwC_CG

Cuts at:   4224    4224 
   Size:       6029
 
SacI G_AGCT'C

Cuts at:   3043    3043 
   Size:       6029
 
SalI G'TCGA_C

Cuts at:   3066    3066 
   Size:       6029
 
SapI GCTCTTCn'nnn_

Cuts at:    183    4052    4262     183 
   Size:       3869     210    1950

  Fragments arranged by size: 

                3869    1950     210
 
SbfI CC_TGCA'GG

Cuts at:   3076    3076 
   Size:       6029
 
ScaI AGT'ACT

Cuts at:   2247    5546    2247 
   Size:       3299    2730
 
SexAI A'CCwGG_T

Cuts at:   2417    2417 
   Size:       6029
 
SfcI C'TryA_G

Cuts at:     33     871    1070    1538    2235    2919    3072    3757    4725 
   Size:        838     199     468     697     684     153     685     968

Cuts at:   4725    4916      33 
   Size:        191    1146

  Fragments arranged by size: 

                1146     968     838     697     685     684     468     199
                 191     153
 
SfoI GGC'GCC

Cuts at:    126    3461    3709     126 
   Size:       3335     248    2446

  Fragments arranged by size: 

                3335    2446     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6029
 
SmaI CCC'GGG

Cuts at:   3051    3051 
   Size:       6029
 
SmlI C'TyrA_G

Cuts at:    414     550     585    1969    3184    4099    4813    5075     414 
   Size:        136      35    1384    1215     915     714     262    1368

  Fragments arranged by size: 

                1384    1368    1215     915     714     262     136      35
            
 
SnaBI TAC'GTA

Cuts at:   1227    1227 
   Size:       6029
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6029
 
SphI G_CATG'C

Cuts at:   1383    3082    3582    1383 
   Size:       1699     500    3830

  Fragments arranged by size: 

                3830    1699     500
 
SspI AAT'ATT

Cuts at:   1735    2052    2110    1735 
   Size:        317      58    5654

  Fragments arranged by size: 

                5654     317      58
 
StyI C'CwwG_G

Cuts at:    578     605    1019    2034    2297    3163    3517     578 
   Size:         27     414    1015     263     866     354    3090

  Fragments arranged by size: 

                3090    1015     866     414     354     263      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1870    3612    3745    4204    4413    1870 
   Size:       1742     133     459     209    3486

  Fragments arranged by size: 

                3486    1742     459     209     133
 
TatI w'GTAC_w

Cuts at:    833    1467    1758    2245    2255    5544     833 
   Size:        634     291     487      10    3289    1318

  Fragments arranged by size: 

                3289    1318     634     487     291      10
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    206     238    1508    1540    5546    5578     206 
   Size:         32    1270      32    4006      32     657

  Fragments arranged by size: 

                4006    1270     657      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3019    3826    3019 
   Size:        807    5222
 
XbaI T'CTAG_A

Cuts at:   3060    3060 
   Size:       6029
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3524    3524 
   Size:       6029
 
XmaI C'CCGG_G

Cuts at:   3049    3049 
   Size:       6029
 
ZraI GAC'GTC

Cuts at:   1302    2649    1302 
   Size:       1347    4682
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstYI  BstZ17I     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI     PsiI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AfeI     AgeI     AleI     AscI    AvrII    BbvCI     BcgI    BfrBI
     BglI     BmtI   Bpu10I    BsiWI    BsrGI   BssHII   BstEII    BstXI
   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI
    PpuMI    PshAI    PspXI     PsrI    SacII    SanDI     SfiI    SgrAI
     SrfI     StuI     SwaI     XhoI     XmnI
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