Restriction map for pF1KB7006
 (Linear) (Six-base) MAPSORT of: KIBB7006.seq  Check: 2051  from: 1  to: 3198



KIBB7006.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

Acc65I G'GTAC_C

Cuts at:      0    2824    3191    3198 
   Size:       2824     367       7

  Fragments arranged by size: 

                2824     367       7
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     809    1259    2159    2618    3143    3198 
   Size:        268     541     450     900     459     525      55

  Fragments arranged by size: 

                 900     541     525     459     450     268      55
 
AgeI A'CCGG_T

Cuts at:      0    1038    3198 
   Size:       1038    2160
 
AleI CACnn'nnGTG

Cuts at:      0    1503    3198 
   Size:       1503    1695
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1166    2797    3198 
   Size:       1166    1631     401

  Fragments arranged by size: 

                1631    1166     401
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2317    2349    2635    2667    3198 
   Size:       2317      32     286      32     531

  Fragments arranged by size: 

                2317     531     286      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     307    1077    1416    2244    3154    3198 
   Size:        307     770     339     828     910      44

  Fragments arranged by size: 

                 910     828     770     339     307      44
 
ApaLI G'TGCA_C

Cuts at:      0    2913    3198 
   Size:       2913     285
 
ApoI r'AATT_y

Cuts at:      0     419    2316    3140    3198 
   Size:        419    1897     824      58

  Fragments arranged by size: 

                1897     824     419      58
 
AseI AT'TA_AT

Cuts at:      0     722    3198 
   Size:        722    2476
 
AvaI C'yCGr_G

Cuts at:      0     311    1376    2003    3198 
   Size:        311    1065     627    1195

  Fragments arranged by size: 

                1195    1065     627     311
 
AvrII C'CTAG_G

Cuts at:      0    2617    3198 
   Size:       2617     581
 
BamHI G'GATC_C

Cuts at:      0    1030    2285    2325    2468    3198 
   Size:       1030    1255      40     143     730

  Fragments arranged by size: 

                1255    1030     730     143      40
 
BanI G'GyrC_C

Cuts at:      0    1842    2824    3191    3198 
   Size:       1842     982     367       7

  Fragments arranged by size: 

                1842     982     367       7
 
BanII G_rGCy'C

Cuts at:      0     545    3198 
   Size:        545    2653
 
BbeI G_GCGC'C

Cuts at:      0    1846    3198 
   Size:       1846    1352
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1353    2002    3198 
   Size:       1353     649    1196

  Fragments arranged by size: 

                1353    1196     649
 
BbvCI CC'TCA_GC

Cuts at:      0     386    3198 
   Size:        386    2812
 
BclI T'GATC_A

Cuts at:      0     996    3198 
   Size:        996    2202
 
BglI GCCn_nnn'nGGC

Cuts at:      0     142    1611    1663    1936    3198 
   Size:        142    1469      52     273    1262

  Fragments arranged by size: 

                1469    1262     273     142      52
 
BglII A'GATC_T

Cuts at:      0      56     902    1338    1617    3198 
   Size:         56     846     436     279    1581

  Fragments arranged by size: 

                1581     846     436     279      56
 
BlpI GC'TnA_GC

Cuts at:      0    1058    3198 
   Size:       1058    2140
 
Bme1580I G_kGCm'C

Cuts at:      0    2838    2917    3198 
   Size:       2838      79     281

  Fragments arranged by size: 

                2838     281      79
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58    1352    3198 
   Size:         58    1294    1846

  Fragments arranged by size: 

                1846    1294      58
 
Bpu10I CC'TnA_GC

Cuts at:      0     386    2657    3198 
   Size:        386    2271     541

  Fragments arranged by size: 

                2271     541     386
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1498    3198 
   Size:       1498    1700
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2031    2080    3198 
   Size:       2031      49    1118

  Fragments arranged by size: 

                2031    1118      49
 
BsaHI Gr'CG_yC

Cuts at:      0    1843    3198 
   Size:       1843    1355
 
BsaWI w'CCGG_w

Cuts at:      0    1038    1203    2282    3198 
   Size:       1038     165    1079     916

  Fragments arranged by size: 

                1079    1038     916     165
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1794    1824    1830    1860    3198 
   Size:       1794      30       6      30    1338

  Fragments arranged by size: 

                1794    1338      30      30       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      97    2567    3055    3198 
   Size:         97    2470     488     143

  Fragments arranged by size: 

                2470     488     143      97
 
BseYI C'CCAG_C

Cuts at:      0    1658    2251    2978    3015    3170    3198 
   Size:       1658     593     727      37     155      28

  Fragments arranged by size: 

                1658     727     593     155      37      28
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     959    3198 
   Size:        160     799    2239

  Fragments arranged by size: 

                2239     799     160
 
BsiEI CG_ry'CG

Cuts at:      0      26     149    1931    3198 
   Size:         26     123    1782    1267

  Fragments arranged by size: 

                1782    1267     123      26
 
BsiHKAI G_wGCw'C

Cuts at:      0     545     560    1088    1713    2917    3198 
   Size:        545      15     528     625    1204     281

  Fragments arranged by size: 

                1204     625     545     528     281      15
 
BsmI GAATG_Cn'

Cuts at:      0     252    1979    3198 
   Size:        252    1727    1219

  Fragments arranged by size: 

                1727    1219     252
 
Bsp1286I G_dGCh'C

Cuts at:      0     545     560    1088    1713    2838    2917    3198 
   Size:        545      15     528     625    1125      79     281

  Fragments arranged by size: 

                1125     625     545     528     281      79      15
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     371    1053    1424    3198 
   Size:        371     682     371    1774

  Fragments arranged by size: 

                1774     682     371     371
 
BsrDI GCAATG_nn'

Cuts at:      0    1148    2754    2756    3198 
   Size:       1148    1606       2     442

  Fragments arranged by size: 

                1606    1148     442       2
 
BsrFI r'CCGG_y

Cuts at:      0      42    1038    1455    3198 
   Size:         42     996     417    1743

  Fragments arranged by size: 

                1743     996     417      42
 
BsrGI T'GTAC_A

Cuts at:      0    1470    3198 
   Size:       1470    1728
 
BssSI C'ACGA_G

Cuts at:      0      79    1706    3198 
   Size:         79    1627    1492

  Fragments arranged by size: 

                1627    1492      79
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1416    3198 
   Size:       1416    1782
 
BstBI TT'CG_AA

Cuts at:      0    3144    3198 
   Size:       3144      54
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1395    2775    3015    3198 
   Size:       1395    1380     240     183

  Fragments arranged by size: 

                1395    1380     240     183
 
BstYI r'GATC_y

Cuts at:      0      56     902    1030    1188    1338    1617    1743    2285 
   Size:         56     846     128     158     150     279     126     542

Cuts at:   2285    2325    2468    3198 
   Size:         40     143     730

  Fragments arranged by size: 

                 846     730     542     279     158     150     143     128
                 126      56      40
 
Bsu36I CC'TnA_GG

Cuts at:      0    1700    2510    3198 
   Size:       1700     810     688

  Fragments arranged by size: 

                1700     810     688
 
BtgI C'CryG_G

Cuts at:      0     128    1400    1533    1838    3198 
   Size:        128    1272     133     305    1360

  Fragments arranged by size: 

                1360    1272     305     133     128
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2844    3198 
   Size:       2844     354
 
BtsI GCAGTG_nn'

Cuts at:      0    1835    2826    2917    3163    3198 
   Size:       1835     991      91     246      35

  Fragments arranged by size: 

                1835     991     246      91      35
 
ClaI AT'CG_AT

Cuts at:      0    1163    2108    3198 
   Size:       1163     945    1090

  Fragments arranged by size: 

                1163    1090     945
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2390    2425    3198 
   Size:       2390      35     773

  Fragments arranged by size: 

                2390     773      35
 
DraI TTT'AAA

Cuts at:      0     680    3198 
   Size:        680    2518
 
DraIII CAC_nnn'GTG

Cuts at:      0      69     564    3198 
   Size:         69     495    2634

  Fragments arranged by size: 

                2634     495      69
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1537    3198 
   Size:       1537    1661
 
EaeI y'GGCC_r

Cuts at:      0     131     146     512    1043    1385    1397    1442    1457 
   Size:        131      15     366     531     342      12      45      15

Cuts at:   1457    1568    1928    2222    2483    2891    3198 
   Size:        111     360     294     261     408     307

  Fragments arranged by size: 

                 531     408     366     360     342     307     294     261
                 131     111      45      15      15      12
 
EagI C'GGCC_G

Cuts at:      0     146    1928    3198 
   Size:        146    1782    1270

  Fragments arranged by size: 

                1782    1270     146
 
EarI CTCTTCn'nnn_

Cuts at:      0     205     793    1208    1862    2642    3198 
   Size:        205     588     415     654     780     556

  Fragments arranged by size: 

                 780     654     588     556     415     205
 
EcoICRI GAG'CTC

Cuts at:      0     543    3198 
   Size:        543    2655
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     268     809    1259    1352    2159    2618    3143 
   Size:         58     210     541     450      93     807     459     525

Cuts at:   3143    3198 
   Size:         55

  Fragments arranged by size: 

                 807     541     525     459     450     210      93      58
                  55
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2944    3198 
   Size:       2944     254
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1603    2607    3198 
   Size:       1603    1004     591

  Fragments arranged by size: 

                1603    1004     591
 
EcoRI G'AATT_C

Cuts at:      0    2316    3198 
   Size:       2316     882
 
EcoRV GAT'ATC

Cuts at:      0    2464    3198 
   Size:       2464     734
 
HaeII r_GCGC'y

Cuts at:      0    1846    3198 
   Size:       1846    1352
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     283     315     981    1013    1500    1532    2143    2175 
   Size:        283      32     666      32     487      32     611      32

Cuts at:   2175    2453    2485    3028    3060    3198 
   Size:        278      32     543      32     138

  Fragments arranged by size: 

                 666     611     543     487     283     278     138      32
                  32      32      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     259     674    1915    3198 
   Size:        259     415    1241    1283

  Fragments arranged by size: 

                1283    1241     415     259
 
KasI G'GCGC_C

Cuts at:      0    1842    3198 
   Size:       1842    1356
 
KpnI G_GTAC'C

Cuts at:      0    2828    3195    3198 
   Size:       2828     367       3

  Fragments arranged by size: 

                2828     367       3
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     150    2147    2450    2529    2580    3198 
   Size:        150    1997     303      79      51     618

  Fragments arranged by size: 

                1997     618     303     150      79      51
 
MscI TGG'CCA

Cuts at:      0     514    1045    1387    1399    1444    2224    2485    2893 
   Size:        514     531     342      12      45     780     261     408

Cuts at:   2893    3198 
   Size:        305

  Fragments arranged by size: 

                 780     531     514     408     342     305     261      45
                  12
 
MslI CAynn'nnrTG

Cuts at:      0    1503    3061    3198 
   Size:       1503    1558     137

  Fragments arranged by size: 

                1558    1503     137
 
MspA1I CmG'CkG

Cuts at:      0    1468    1503    1650    1662    3198 
   Size:       1468      35     147      12    1536

  Fragments arranged by size: 

                1536    1468     147      35      12
 
NarI GG'CG_CC

Cuts at:      0    1843    3198 
   Size:       1843    1355
 
PasI CC'CwG_GG

Cuts at:      0     871    3198 
   Size:        871    2327
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     845    1198    3198 
   Size:        845     353    2000

  Fragments arranged by size: 

                2000     845     353
 
PfoI T'CCnGG_A

Cuts at:      0      35    2450    3198 
   Size:         35    2415     748

  Fragments arranged by size: 

                2415     748      35
 
PpuMI rG'GwC_Cy

Cuts at:      0    2607    3198 
   Size:       2607     591
 
PspXI vC'TCGA_Gb

Cuts at:      0    1376    3198 
   Size:       1376    1822
 
PstI C_TGCA'G

Cuts at:      0    1412    1421    3198 
   Size:       1412       9    1777

  Fragments arranged by size: 

                1777    1412       9
 
PvuI CG_AT'CG

Cuts at:      0      26    3198 
   Size:         26    3172
 
PvuII CAG'CTG

Cuts at:      0    1468    1650    1662    3198 
   Size:       1468     182      12    1536

  Fragments arranged by size: 

                1536    1468     182      12
 
SacI G_AGCT'C

Cuts at:      0     545    3198 
   Size:        545    2653
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1208    3198 
   Size:       1208    1990
 
SexAI A'CCwGG_T

Cuts at:      0      65    3198 
   Size:         65    3133
 
SfcI C'TryA_G

Cuts at:      0     698    1408    1417    3198 
   Size:        698     710       9    1781

  Fragments arranged by size: 

                1781     710     698       9
 
SfoI GGC'GCC

Cuts at:      0    1844    3198 
   Size:       1844    1354
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1038    3198 
   Size:       1038    2160
 
SmlI C'TyrA_G

Cuts at:      0     311    1376    1477    3198 
   Size:        311    1065     101    1721

  Fragments arranged by size: 

                1721    1065     311     101
 
StuI AGG'CCT

Cuts at:      0    1987    2622    2869    3198 
   Size:       1987     635     247     329

  Fragments arranged by size: 

                1987     635     329     247
 
StyI C'CwwG_G

Cuts at:      0     660    1302    1382    2486    2617    2852    2864    2894 
   Size:        660     642      80    1104     131     235      12      30

Cuts at:   2894    3198 
   Size:        304

  Fragments arranged by size: 

                1104     660     642     304     235     131      80      30
                  12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1531    1652    2992    3198 
   Size:       1531     121    1340     206

  Fragments arranged by size: 

                1531    1340     206     121
 
TatI w'GTAC_w

Cuts at:      0    1470    3198 
   Size:       1470    1728
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1830    1862    3198 
   Size:       1830      32    1336

  Fragments arranged by size: 

                1830    1336      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1437    1821    2163    2385    3063    3198 
   Size:       1437     384     342     222     678     135

  Fragments arranged by size: 

                1437     678     384     342     222     135
 
XhoI C'TCGA_G

Cuts at:      0     311    1376    3198 
   Size:        311    1065    1822

  Fragments arranged by size: 

                1822    1065     311
 
XmnI GAAnn'nnTTC

Cuts at:      0    1979    2421    3198 
   Size:       1979     442     777

  Fragments arranged by size: 

                1979     777     442
 

 Enzymes that do cut:

   Acc65I     AcuI     AgeI     AleI     AlfI     AloI    AlwNI    ApaLI
     ApoI     AseI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BclI     BglI    BglII     BlpI Bme1580I     BpmI
   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
    HaeII    Hin4I  HindIII     KasI     KpnI     MmeI     MscI     MslI
   MspA1I     NarI     PasI    PflMI     PfoI    PpuMI    PspXI     PstI
     PvuI    PvuII     SacI     SapI    SexAI     SfcI     SfoI    SgrAI
     SmlI     StuI     StyI    TaqII     TatI     TstI     XcmI     XhoI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AhdI
     ApaI     AscI    AsiSI     BaeI     BcgI    BciVI    BfrBI    BmgBI
     BmrI     BmtI     BplI    BsaAI    BsaBI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI   BssHII   BstEII  BstZ17I     EciI     FalI     FseI
     FspI    FspAI   HincII     HpaI     MfeI     MluI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI     PsrI
    RsrII    SacII     SalI    SanDI     SbfI     ScaI     SfiI     SgfI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI  Tth111I
     XbaI     XmaI     ZraI
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