Restriction map for pF1KB5664
 (Linear) (Six-base) MAPSORT of: KIBB5664.seq  Check: 2222  from: 1  to: 1134



KIBB5664.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     385    1134 
   Size:        385     749
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115    1096    1134 
   Size:        115     981      38

  Fragments arranged by size: 

                 981     115      38
 
AleI CACnn'nnGTG

Cuts at:      0     127    1134 
   Size:        127    1007
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     928    1134 
   Size:        928     206
 
ApaI G_GGCC'C

Cuts at:      0     969    1134 
   Size:        969     165
 
ApoI r'AATT_y

Cuts at:      0     205    1134 
   Size:        205     929
 
BanI G'GyrC_C

Cuts at:      0     452     505    1134 
   Size:        452      53     629

  Fragments arranged by size: 

                 629     452      53
 
BanII G_rGCy'C

Cuts at:      0     969    1134 
   Size:        969     165
 
BbeI G_GCGC'C

Cuts at:      0     509    1134 
   Size:        509     625
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     310     453    1064    1134 
   Size:        310     143     611      70

  Fragments arranged by size: 

                 611     310     143      70
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     138     172     336     370    1134 
   Size:        138      34     164      34     764

  Fragments arranged by size: 

                 764     164     138      34      34
 
BclI T'GATC_A

Cuts at:      0     419    1134 
   Size:        419     715
 
BglI GCCn_nnn'nGGC

Cuts at:      0     367    1134 
   Size:        367     767
 
Bme1580I G_kGCm'C

Cuts at:      0     823     969    1134 
   Size:        823     146     165

  Fragments arranged by size: 

                 823     165     146
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      10    1134 
   Size:         10    1124
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      40    1134 
   Size:         40    1094
 
BsaAI yAC'GTr

Cuts at:      0      72    1134 
   Size:         72    1062
 
BsaBI GATnn'nnATC

Cuts at:      0     187    1134 
   Size:        187     947
 
BsaHI Gr'CG_yC

Cuts at:      0     370     440     506    1134 
   Size:        370      70      66     628

  Fragments arranged by size: 

                 628     370      70      66
 
BseYI C'CCAG_C

Cuts at:      0     501    1134 
   Size:        501     633
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     832    1134 
   Size:        520     312     302

  Fragments arranged by size: 

                 520     312     302
 
BsiHKAI G_wGCw'C

Cuts at:      0     134     866    1134 
   Size:        134     732     268

  Fragments arranged by size: 

                 732     268     134
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      28    1134 
   Size:         28    1106
 
Bsp1286I G_dGCh'C

Cuts at:      0     134     823     866     969    1134 
   Size:        134     689      43     103     165

  Fragments arranged by size: 

                 689     165     134     103      43
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     263     385    1070    1134 
   Size:        263     122     685      64

  Fragments arranged by size: 

                 685     263     122      64
 
BsrBI CCG'CTC

Cuts at:      0     405     708    1134 
   Size:        405     303     426

  Fragments arranged by size: 

                 426     405     303
 
BsrDI GCAATG_nn'

Cuts at:      0     488     587    1134 
   Size:        488      99     547

  Fragments arranged by size: 

                 547     488      99
 
BsrFI r'CCGG_y

Cuts at:      0      30     262    1134 
   Size:         30     232     872

  Fragments arranged by size: 

                 872     232      30
 
BstEII G'GTnAC_C

Cuts at:      0     624    1134 
   Size:        624     510
 
BstYI r'GATC_y

Cuts at:      0    1121    1134 
   Size:       1121      13
 
Bsu36I CC'TnA_GG

Cuts at:      0     337    1134 
   Size:        337     797
 
BtgI C'CryG_G

Cuts at:      0     323     713     731    1134 
   Size:        323     390      18     403

  Fragments arranged by size: 

                 403     390     323      18
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     252     378     741    1134 
   Size:        252     126     363     393

  Fragments arranged by size: 

                 393     363     252     126
 
BtsI GCAGTG_nn'

Cuts at:      0     718    1134 
   Size:        718     416
 
EaeI y'GGCC_r

Cuts at:      0     260     389     614     686     899    1134 
   Size:        260     129     225      72     213     235

  Fragments arranged by size: 

                 260     235     225     213     129      72
 
EarI CTCTTCn'nnn_

Cuts at:      0     139     781     790     835    1134 
   Size:        139     642       9      45     299

  Fragments arranged by size: 

                 642     299     139      45       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     861    1134 
   Size:        861     273
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      10     115    1096    1134 
   Size:         10     105     981      38

  Fragments arranged by size: 

                 981     105      38      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     340     832    1134 
   Size:        340     492     302

  Fragments arranged by size: 

                 492     340     302
 
EcoO109I rG'GnC_Cy

Cuts at:      0     965    1134 
   Size:        965     169
 
HaeII r_GCGC'y

Cuts at:      0     509    1134 
   Size:        509     625
 
HincII GTy'rAC

Cuts at:      0     282    1134 
   Size:        282     852
 
KasI G'GCGC_C

Cuts at:      0     505    1134 
   Size:        505     629
 
MscI TGG'CCA

Cuts at:      0     391     688     901    1134 
   Size:        391     297     213     233

  Fragments arranged by size: 

                 391     297     233     213
 
MslI CAynn'nnrTG

Cuts at:      0     127     289     622     796    1134 
   Size:        127     162     333     174     338

  Fragments arranged by size: 

                 338     333     174     162     127
 
NaeI GCC'GGC

Cuts at:      0     264    1134 
   Size:        264     870
 
NarI GG'CG_CC

Cuts at:      0     506    1134 
   Size:        506     628
 
NcoI C'CATG_G

Cuts at:      0     713    1134 
   Size:        713     421
 
NgoMIV G'CCGG_C

Cuts at:      0     262    1134 
   Size:        262     872
 
NspI r_CATG'y

Cuts at:      0     222    1134 
   Size:        222     912
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      72     329    1134 
   Size:         72     257     805

  Fragments arranged by size: 

                 805     257      72
 
PspOMI G'GGCC_C

Cuts at:      0     965    1134 
   Size:        965     169
 
PstI C_TGCA'G

Cuts at:      0     151     382     808    1134 
   Size:        151     231     426     326

  Fragments arranged by size: 

                 426     326     231     151
 
RsrII CG'GwC_CG

Cuts at:      0     728    1134 
   Size:        728     406
 
SapI GCTCTTCn'nnn_

Cuts at:      0     139    1134 
   Size:        139     995
 
SbfI CC_TGCA'GG

Cuts at:      0     808    1134 
   Size:        808     326
 
SfcI C'TryA_G

Cuts at:      0     147     378     804    1134 
   Size:        147     231     426     330

  Fragments arranged by size: 

                 426     330     231     147
 
SfoI GGC'GCC

Cuts at:      0     507    1134 
   Size:        507     627
 
StuI AGG'CCT

Cuts at:      0     103     432    1134 
   Size:        103     329     702

  Fragments arranged by size: 

                 702     329     103
 
StyI C'CwwG_G

Cuts at:      0     363     713    1134 
   Size:        363     350     421

  Fragments arranged by size: 

                 421     363     350
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     993    1134 
   Size:        993     141
 
TatI w'GTAC_w

Cuts at:      0     221     584    1134 
   Size:        221     363     550

  Fragments arranged by size: 

                 550     363     221
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     764     796    1134 
   Size:        764      32     338

  Fragments arranged by size: 

                 764     338      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     307    1134 
   Size:        307     827
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AlfI     ApaI     ApoI     BanI    BanII
     BbeI     BbsI     BcgI     BclI     BglI Bme1580I     BpmI     BsaI
    BsaAI    BsaBI    BsaHI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspMI    BsrBI    BsrDI    BsrFI   BstEII    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I
    HaeII   HincII     KasI     MscI     MslI     NaeI     NarI     NcoI
   NgoMIV     NspI    PflMI   PspOMI     PstI    RsrII     SapI     SbfI
     SfcI     SfoI     StuI     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BbvCI    BciVI    BfrBI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaWI    BsaXI
    BseRI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI  BstZ17I     ClaI    CspCI     DraI
   DraIII     DrdI     EagI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI   MspA1I     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    PvuII     SacI    SacII     SalI
    SanDI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com