Restriction map for pF1KB5645
 (Circular) (Six-base) MAPSORT of: pF1KB5645.seq  Check: 660  from: 1  to: 6623



pF1KB5645  6623 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   3341    3341 
   Size:       6623
 
AatII G_ACGT'C

Cuts at:    650     650 
   Size:       6623
 
AccI GT'mk_AC

Cuts at:   2555    3546    3661    2555 
   Size:        991     115    5517

  Fragments arranged by size: 

                5517     991     115
 
Acc65I G'GTAC_C

Cuts at:   3639    3639 
   Size:       6623
 
AclI AA'CG_TT

Cuts at:   6463    6463 
   Size:       6623
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    441    1034    3114    4448    4880    5627    5895     441 
   Size:        593    2080    1334     432     747     268    1169

  Fragments arranged by size: 

                2080    1334    1169     747     593     432     268
 
AflIII A'CryG_T

Cuts at:   1456    3975    5079    6056    1456 
   Size:       2519    1104     977    2023

  Fragments arranged by size: 

                2519    2023    1104     977
 
AgeI A'CCGG_T

Cuts at:   1925    5011    1925 
   Size:       3086    3537
 
AhdI GACnn_n'nnGTC

Cuts at:    130     130 
   Size:       6623
 
AleI CACnn'nnGTG

Cuts at:    976    1928     976 
   Size:        952    5671
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1906    2860    1906 
   Size:        954    5669
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1263    1295    1263 
   Size:         32    6591
 
AlwNI CAG_nnn'CTG

Cuts at:    178    1198    3322    3928    5495     178 
   Size:       1020    2124     606    1567    1306

  Fragments arranged by size: 

                2124    1567    1306    1020     606
 
ApaI G_GGCC'C

Cuts at:    262     603     262 
   Size:        341    6282
 
ApaLI G'TGCA_C

Cuts at:   1863    5393    1863 
   Size:       3530    3093
 
ApoI r'AATT_y

Cuts at:   1301    2345    3627    5976    6232    1301 
   Size:       1044    1282    2349     256    1692

  Fragments arranged by size: 

                2349    1692    1282    1044     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6623
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6623
 
AvaI C'yCGr_G

Cuts at:    407    3282    3643     407 
   Size:       2875     361    3387

  Fragments arranged by size: 

                3387    2875     361
 
BamHI G'GATC_C

Cuts at:   1481    3648    1481 
   Size:       2167    4456
 
BanI G'GyrC_C

Cuts at:    157     242     484    3136    3639    4053    4301    4336     157 
   Size:         85     242    2652     503     414     248      35    2444

  Fragments arranged by size: 

                2652    2444     503     414     248     242      85      35
            
 
BanII G_rGCy'C

Cuts at:    262     603    1354    3273    3637    4667     262 
   Size:        341     751    1919     364    1030    2218

  Fragments arranged by size: 

                2218    1919    1030     751     364     341
 
BbeI G_GCGC'C

Cuts at:    246    4057    4305     246 
   Size:       3811     248    2564

  Fragments arranged by size: 

                3811    2564     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    409     447     599     657     785    2294    3555     409 
   Size:         38     152      58     128    1509    1261    3477

  Fragments arranged by size: 

                3477    1509    1261     152     128      58      38
 
BbvCI CC'TCA_GC

Cuts at:    496    1042     496 
   Size:        546    6077
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   3154    3188    3379    3413    3154 
   Size:         34     191      34    6364

  Fragments arranged by size: 

                6364     191      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2533    3271    4521    5282    6623    2533 
   Size:        738    1250     761    1341    2533

  Fragments arranged by size: 

                2533    1341    1250     761     738
 
BclI T'GATC_A

Cuts at:   1472    4143    1472 
   Size:       2671    3952
 
BfrBI ATG'CAT

Cuts at:    147     147 
   Size:       6623
 
BglI GCCn_nnn'nGGC

Cuts at:   2752    2752 
   Size:       6623
 
BglII A'GATC_T

Cuts at:   1724    4138    6175    1724 
   Size:       2414    2037    2172

  Fragments arranged by size: 

                2414    2172    2037
 
BlpI GC'TnA_GC

Cuts at:   3735    3735 
   Size:       6623
 
Bme1580I G_kGCm'C

Cuts at:    262     415     487     603    1867    2741    2849    4248    4341 
   Size:        153      72     116    1264     874     108    1399      93

Cuts at:   4341    5397     262 
   Size:       1056    1488

  Fragments arranged by size: 

                1488    1399    1264    1056     874     153     116     108
                  93      72
 
BmrI ACTGGGnnnn_n'

Cuts at:   1674    2198    3045    3234    3258    3835    3916    4251    6395 
   Size:        524     847     189      24     577      81     335    2144

Cuts at:   6395    1674 
   Size:       1902

  Fragments arranged by size: 

                2144    1902     847     577     524     335     189      81
                  24
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1067    1290    1371    4990    1067 
   Size:        223      81    3619    2700

  Fragments arranged by size: 

                3619    2700     223      81
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    448     789    1440     448 
   Size:        341     651    5631

  Fragments arranged by size: 

                5631     651     341
 
Bpu10I CC'TnA_GC

Cuts at:    496    1042    3272     496 
   Size:        546    2230    3847

  Fragments arranged by size: 

                3847    2230     546
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3554    3799    4678    5170    5468    3554 
   Size:        245     879     492     298    4709

  Fragments arranged by size: 

                4709     879     492     298     245
 
BsaI GGTCTCn'nnnn_

Cuts at:    668    1259    1540    2414    2962    6327     668 
   Size:        591     281     874     548    3365     964

  Fragments arranged by size: 

                3365     964     874     591     548     281
 
BsaAI yAC'GTr

Cuts at:    525    3976     525 
   Size:       3451    3172
 
BsaBI GATnn'nnATC

Cuts at:      6    4137    6174       6 
   Size:       4131    2037     455

  Fragments arranged by size: 

                4131    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    131     243     647     716    1568    2492    3438    4054    4302 
   Size:        112     404      69     852     924     946     616     248

Cuts at:   4302    5004    6500     131 
   Size:        702    1496     254

  Fragments arranged by size: 

                1496     946     924     852     702     616     404     254
                 248     112      69
 
BsaWI w'CCGG_w

Cuts at:   1925    2631    3812    4333    5011    5285    5432    5843    1925 
   Size:        706    1181     521     678     274     147     411    2705

  Fragments arranged by size: 

                2705    1181     706     678     521     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1625    1655    1625 
   Size:         30    6593
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    814    1344    1649     814 
   Size:        530     305    5788

  Fragments arranged by size: 

                5788     530     305
 
BseYI C'CCAG_C

Cuts at:    276     602    1228    1501    1589    2385    4048    4806    5383 
   Size:        326     626     273      88     796    1663     758     577

Cuts at:   5383    5879     276 
   Size:        496    1020

  Fragments arranged by size: 

                1663    1020     796     758     626     577     496     326
                 273      88
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    607    1098    2040     607 
   Size:        491     942    5190

  Fragments arranged by size: 

                5190     942     491
 
BsiEI CG_ry'CG

Cuts at:    104    1317    4165    4211    5419     104 
   Size:       1213    2848      46    1208    1308

  Fragments arranged by size: 

                2848    1308    1213    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1867    1925    3332    3637    4415    4605    5397    1867 
   Size:         58    1407     305     778     190     792    3093

  Fragments arranged by size: 

                3093    1407     792     778     305     190      58
 
BsmI GAATG_Cn'

Cuts at:    167     639    1213    2813     167 
   Size:        472     574    1600    3977

  Fragments arranged by size: 

                3977    1600     574     472
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1663    2483    2510    3445    1663 
   Size:        820      27     935    4841

  Fragments arranged by size: 

                4841     935     820      27
 
Bsp1286I G_dGCh'C

Cuts at:    262     415     487     603    1354    1867    1925    2741    2849 
   Size:        153      72     116     751     513      58     816     108

Cuts at:   2849    3273    3332    3637    4248    4341    4415    4605    4667 
   Size:        424      59     305     611      93      74     190      62

Cuts at:   4667    5397     262 
   Size:        730    1488

  Fragments arranged by size: 

                1488     816     751     730     611     513     424     305
                 190     153     116     108      93      74      72      62
                  59      58
 
BspEI T'CCGG_A

Cuts at:   2631    5843    2631 
   Size:       3212    3411
 
BspHI T'CATG_A

Cuts at:   5799    5799 
   Size:       6623
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1381    3341    3673    3887    4189    4570    1381 
   Size:       1960     332     214     302     381    3434

  Fragments arranged by size: 

                3434    1960     381     332     302     214
 
BsrBI CCG'CTC

Cuts at:     50     432    1319    4915    4969    6453    6618      50 
   Size:        382     887    3596      54    1484     165      55

  Fragments arranged by size: 

                3596    1484     887     382     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1302    2166    4535    1302 
   Size:        864    2369    3390

  Fragments arranged by size: 

                3390    2369     864
 
BsrFI r'CCGG_y

Cuts at:    556    1780    1925    1981    4621    4802    5011     556 
   Size:       1224     145      56    2640     181     209    2168

  Fragments arranged by size: 

                2640    2168    1224     209     181     145      56
 
BsrGI T'GTAC_A

Cuts at:    608     608 
   Size:       6623
 
BssSI C'ACGA_G

Cuts at:   2842    3264    4894    5252    2842 
   Size:        422    1630     358    4213

  Fragments arranged by size: 

                4213    1630     422     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1697    1862    2171    4000    1697 
   Size:        165     309    1829    4320

  Fragments arranged by size: 

                4320    1829     309     165
 
BstBI TT'CG_AA

Cuts at:   4984    4984 
   Size:       6623
 
BstEII G'GTnAC_C

Cuts at:   1601    1601 
   Size:       6623
 
BstXI CCAn_nnnn'nTGG

Cuts at:    114     678     114 
   Size:        564    6059
 
BstYI r'GATC_y

Cuts at:      1    1481    1724    3612    3648    4115    4138    4473    4719 
   Size:       1480     243    1888      36     467      23     335     246

Cuts at:   4719    5731    5817    5829    6175       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1888    1480    1012     467     449     346     335     246
                 243      86      36      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    388     787     388 
   Size:        399    6224
 
BtgI C'CryG_G

Cuts at:    107    1258    1435    1606    2018     107 
   Size:       1151     177     171     412    4712

  Fragments arranged by size: 

                4712    1151     412     177     171
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    672     687    2891    4573    4754    4917     672 
   Size:         15    2204    1682     181     163    2378

  Fragments arranged by size: 

                2378    2204    1682     181     163      15
 
BtsI GCAGTG_nn'

Cuts at:   2241    3866    2241 
   Size:       1625    4998
 
ClaI AT'CG_AT

Cuts at:   2933    2933 
   Size:       6623
 
DraI TTT'AAA

Cuts at:   1404    3622    1404 
   Size:       2218    4405
 
DraIII CAC_nnn'GTG

Cuts at:    528    1121    3386     528 
   Size:        593    2265    3765

  Fragments arranged by size: 

                3765    2265     593
 
DrdI GACnn_nn'nnGTC

Cuts at:   4329    5187    4329 
   Size:        858    5765
 
EaeI y'GGCC_r

Cuts at:    447     558     680     885    1031    1314    1998    2021    2225 
   Size:        111     122     205     146     283     684      23     204

Cuts at:   2225    2703    3369    4208    4382    4773    4800    6145     447 
   Size:        478     666     839     174     391      27    1345     925

  Fragments arranged by size: 

                1345     925     839     684     666     478     391     283
                 205     204     174     146     122     111      27      23
            
 
EagI C'GGCC_G

Cuts at:   1314    4208    1314 
   Size:       2894    3729
 
EarI CTCTTCn'nnn_

Cuts at:    562    1010    4646    4856     562 
   Size:        448    3636     210    2329

  Fragments arranged by size: 

                3636    2329     448     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    197    2968    4831    5141    5287    6433     197 
   Size:       2771    1863     310     146    1146     387

  Fragments arranged by size: 

                2771    1863    1146     387     310     146
 
EcoICRI GAG'CTC

Cuts at:   3635    3635 
   Size:       6623
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    441     448     789    1034    1440    3114    4448    4880    5627 
   Size:          7     341     245     406    1674    1334     432     747

Cuts at:   5627    5895     441 
   Size:        268    1169

  Fragments arranged by size: 

                1674    1334    1169     747     432     406     341     268
                 245       7
 
EcoO109I rG'GnC_Cy

Cuts at:    258     259     599     963    1594    2066    3319    3514    3762 
   Size:          1     340     364     631     472    1253     195     248

Cuts at:   3762     258 
   Size:       3119

  Fragments arranged by size: 

                3119    1253     631     472     364     340     248     195
                   1
 
EcoRI G'AATT_C

Cuts at:   1301    3627    1301 
   Size:       2326    4297
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   4642    4642 
   Size:       6623
 
FspI TGC'GCA

Cuts at:    970    3379    4404     970 
   Size:       2409    1025    3189

  Fragments arranged by size: 

                3189    2409    1025
 
FspAI rTGC'GCAy

Cuts at:   3379    3379 
   Size:       6623
 
HaeII r_GCGC'y

Cuts at:    246    4057    4305    5327    6306     246 
   Size:       3811     248    1022     979     563

  Fragments arranged by size: 

                3811    1022     979     563     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    116     148     686     718     922     954    1237    1269    2507 
   Size:         32     538      32     204      32     283      32    1238

Cuts at:   2507    2539    2635    2667    2982    3014    3425    3457    4465 
   Size:         32      96      32     315      32     411      32    1008

Cuts at:   4465    4495    4497    4527     116 
   Size:         30       2      30    2212

  Fragments arranged by size: 

                2212    1238    1008     538     411     315     283     204
                  96      32      32      32      32      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:   2958    3078    3662    2958 
   Size:        120     584    5919

  Fragments arranged by size: 

                5919     584     120
 
KasI G'GCGC_C

Cuts at:    242    4053    4301     242 
   Size:       3811     248    2564

  Fragments arranged by size: 

                3811    2564     248
 
KpnI G_GTAC'C

Cuts at:   3643    3643 
   Size:       6623
 
MfeI C'AATT_G

Cuts at:   1748    2610    1748 
   Size:        862    5761
 
MluI A'CGCG_T

Cuts at:   6056    6056 
   Size:       6623
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    623    2574    5294    5478     623 
   Size:       1951    2720     184    1768

  Fragments arranged by size: 

                2720    1951    1768     184
 
MscI TGG'CCA

Cuts at:    449     682     887    1033    2227    2705    3371    4384     449 
   Size:        233     205     146    1194     478     666    1013    2688

  Fragments arranged by size: 

                2688    1194    1013     666     478     233     205     146
            
 
MslI CAynn'nnrTG

Cuts at:    152     976    1928    2581    2798    2918    3472    4612    4739 
   Size:        824     952     653     217     120     554    1140     127

Cuts at:   4739     152 
   Size:       2036

  Fragments arranged by size: 

                2036    1140     952     824     653     554     217     127
                 120
 
MspA1I CmG'CkG

Cuts at:    606    1814    2132    2426    2640    3734    4048    4408    5421 
   Size:       1208     318     294     214    1094     314     360    1013

Cuts at:   5421    5666     606 
   Size:        245    1563

  Fragments arranged by size: 

                1563    1208    1094    1013     360     318     314     294
                 245     214
 
NaeI GCC'GGC

Cuts at:   1782    4804    1782 
   Size:       3022    3601
 
NarI GG'CG_CC

Cuts at:    243    4054    4302     243 
   Size:       3811     248    2564

  Fragments arranged by size: 

                3811    2564     248
 
NcoI C'CATG_G

Cuts at:    107    1258    1435    1606     107 
   Size:       1151     177     171    5124

  Fragments arranged by size: 

                5124    1151     177     171
 
NgoMIV G'CCGG_C

Cuts at:   1780    4802    1780 
   Size:       3022    3601
 
NotI GC'GGCC_GC

Cuts at:   1314    1314 
   Size:       6623
 
NsiI A_TGCA'T

Cuts at:    149     149 
   Size:       6623
 
NspI r_CATG'y

Cuts at:    151    1460    2017    3676    4176    5083     151 
   Size:       1309     557    1659     500     907    1691

  Fragments arranged by size: 

                1691    1659    1309     907     557     500
 
PciI A'CATG_T

Cuts at:   1456    5079    1456 
   Size:       3623    3000
 
PflMI CCAn_nnn'nTGG

Cuts at:    178     339    1753     178 
   Size:        161    1414    5048

  Fragments arranged by size: 

                5048    1414     161
 
PfoI T'CCnGG_A

Cuts at:    950    3087     950 
   Size:       2137    4486
 
PmeI GTTT'AAAC

Cuts at:   3622    3622 
   Size:       6623
 
PmlI CAC'GTG

Cuts at:    525     525 
   Size:       6623
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5762    5794    5762 
   Size:         32    6591
 
PpuMI rG'GwC_Cy

Cuts at:   1594    2066    3514    1594 
   Size:        472    1448    4703

  Fragments arranged by size: 

                4703    1448     472
 
PshAI GACnn'nnGTC

Cuts at:   2366    2435    2726    2942    3398    2366 
   Size:         69     291     216     456    5591

  Fragments arranged by size: 

                5591     456     291     216      69
 
PspOMI G'GGCC_C

Cuts at:    258     599     258 
   Size:        341    6282
 
PstI C_TGCA'G

Cuts at:    746     992    2505    2839    3670    4355     746 
   Size:        246    1513     334     831     685    3014

  Fragments arranged by size: 

                3014    1513     831     685     334     246
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6623
 
PvuII CAG'CTG

Cuts at:    606    1814    2640    4048    4408     606 
   Size:       1208     826    1408     360    2821

  Fragments arranged by size: 

                2821    1408    1208     826     360
 
RsrII CG'GwC_CG

Cuts at:   4818    4818 
   Size:       6623
 
SacI G_AGCT'C

Cuts at:   3637    3637 
   Size:       6623
 
SalI G'TCGA_C

Cuts at:   3660    3660 
   Size:       6623
 
SanDI GG'GwC_CC

Cuts at:   2066    2066 
   Size:       6623
 
SapI GCTCTTCn'nnn_

Cuts at:    562    4646    4856     562 
   Size:       4084     210    2329

  Fragments arranged by size: 

                4084    2329     210
 
SbfI CC_TGCA'GG

Cuts at:   3670    3670 
   Size:       6623
 
ScaI AGT'ACT

Cuts at:   6140    6140 
   Size:       6623
 
SexAI A'CCwGG_T

Cuts at:    338    2213     338 
   Size:       1875    4748
 
SfcI C'TryA_G

Cuts at:     33     742     988    2112    2501    2835    3098    3429    3666 
   Size:        709     246    1124     389     334     263     331     237

Cuts at:   3666    4351    5344    5535      33 
   Size:        685     993     191    1121

  Fragments arranged by size: 

                1124    1121     993     709     685     389     334     331
                 263     246     237     191
 
SfoI GGC'GCC

Cuts at:    244    4055    4303     244 
   Size:       3811     248    2564

  Fragments arranged by size: 

                3811    2564     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6623
 
SgrAI Cr'CCGG_yG

Cuts at:   1925    1925 
   Size:       6623
 
SmaI CCC'GGG

Cuts at:    409    3645     409 
   Size:       3236    3387
 
SmlI C'TyrA_G

Cuts at:   3569    3778    4693    5185    5447    3569 
   Size:        209     915     492     262    4745

  Fragments arranged by size: 

                4745     915     492     262     209
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6623
 
SphI G_CATG'C

Cuts at:   3676    4176    3676 
   Size:        500    6123
 
StyI C'CwwG_G

Cuts at:    107     510    1258    1435    1597    1606    2618    3757    4111 
   Size:        403     748     177     162       9    1012    1139     354

Cuts at:   4111     107 
   Size:       2619

  Fragments arranged by size: 

                2619    1139    1012     748     403     354     177     162
                   9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1347    2986    3253    4206    4339    4798    5007    1347 
   Size:       1639     267     953     133     459     209    2963

  Fragments arranged by size: 

                2963    1639     953     459     267     209     133
 
TatI w'GTAC_w

Cuts at:    608    1079    1792    6138     608 
   Size:        471     713    4346    1093

  Fragments arranged by size: 

                4346    1093     713     471
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1512    1544    1809    1841    6140    6172    1512 
   Size:         32     265      32    4299      32    1963

  Fragments arranged by size: 

                4299    1963     265      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   4420    4420 
   Size:       6623
 
XbaI T'CTAG_A

Cuts at:   3654    3654 
   Size:       6623
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    948    1265    2211    2767    4118     948 
   Size:        317     946     556    1351    3453

  Fragments arranged by size: 

                3453    1351     946     556     317
 
XmaI C'CCGG_G

Cuts at:    407    3643     407 
   Size:       3236    3387
 
XmnI GAAnn'nnTTC

Cuts at:   3418    3418 
   Size:       6623
 
ZraI GAC'GTC

Cuts at:    648     648 
   Size:       6623
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    FspAI    HaeII    Hin4I   HincII     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NotI     NsiI     NspI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI     PstI
     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI
     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AscI    AvrII     BaeI    BmgBI     BmtI    BsiWI
   BssHII  BstZ17I    CspCI    EcoNI    EcoRV     FseI  HindIII     HpaI
     NdeI     NheI     NruI     PacI     PasI     PsiI    PspXI     PsrI
    SacII     SfiI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI
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