Restriction map for
pF1KB5266
(Circular) (Six-base) MAPSORT of: pF1KB5266.seq Check: 6853 from: 1 to: 4163
pF1KB5266 4163 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 142 142
Size: 4163
AccI GT'mk_AC
Cuts at: 1201 1201
Size: 4163
Acc65I G'GTAC_C
Cuts at: 1179 1179
Size: 4163
AclI AA'CG_TT
Cuts at: 4003 4003
Size: 4163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 524 1052 1094 1121 1988 2420 2773 3435
Size: 368 528 42 27 867 432 353 662
Cuts at: 3435 156
Size: 884
Fragments arranged by size:
884 867 662 528 432 368 353 42
27
AfeI AGC'GCT
Cuts at: 210 210
Size: 4163
AflIII A'CryG_T
Cuts at: 1515 3315 3596 1515
Size: 1800 281 2082
Fragments arranged by size:
2082 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 340 1144 1468 2906 340
Size: 804 324 1438 1597
Fragments arranged by size:
1597 1438 804 324
ApaLI G'TGCA_C
Cuts at: 3001 3001
Size: 4163
ApoI r'AATT_y
Cuts at: 648 814 1116 1167 3516 3772 648
Size: 166 302 51 2349 256 1039
Fragments arranged by size:
2349 1039 302 256 166 51
AseI AT'TA_AT
Cuts at: 20 20
Size: 4163
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 1183 1183
Size: 4163
BamHI G'GATC_C
Cuts at: 1188 1188
Size: 4163
BanI G'GyrC_C
Cuts at: 1179 1593 1841 1876 1179
Size: 414 248 35 3466
Fragments arranged by size:
3466 414 248 35
BanII G_rGCy'C
Cuts at: 1177 2207 1177
Size: 1030 3133
BbeI G_GCGC'C
Cuts at: 1597 1845 1597
Size: 248 3915
BciVI GTATCCnnnnn_n'
Cuts at: 2061 3117 4163 2061
Size: 1056 1046 2061
Fragments arranged by size:
2061 1056 1046
BclI T'GATC_A
Cuts at: 1683 1683
Size: 4163
BglII A'GATC_T
Cuts at: 1678 3715 1678
Size: 2037 2126
BlpI GC'TnA_GC
Cuts at: 1275 1275
Size: 4163
Bme1580I G_kGCm'C
Cuts at: 1788 1881 3005 1788
Size: 93 1124 2946
Fragments arranged by size:
2946 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1375 1456 1791 3935 1375
Size: 81 335 2144 1603
Fragments arranged by size:
2144 1603 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 4163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1339 2218 2932 3230 1339
Size: 879 714 298 2272
Fragments arranged by size:
2272 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3867 3867
Size: 4163
BsaAI yAC'GTr
Cuts at: 1516 1516
Size: 4163
BsaBI GATnn'nnATC
Cuts at: 6 1677 3714 6
Size: 1671 2037 455
Fragments arranged by size:
2037 1671 455
BsaHI Gr'CG_yC
Cuts at: 124 1594 1842 2544 4040 124
Size: 1470 248 702 1496 247
Fragments arranged by size:
1496 1470 702 248 247
BsaWI w'CCGG_w
Cuts at: 1352 1873 2551 2962 3109 3383 1352
Size: 521 678 411 147 274 2132
Fragments arranged by size:
2132 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1105 1105
Size: 4163
BseYI C'CCAG_C
Cuts at: 1588 2346 3011 3419 1588
Size: 758 665 408 2332
Fragments arranged by size:
2332 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 582 758 582
Size: 176 3987
BsiEI CG_ry'CG
Cuts at: 104 1705 1751 2981 104
Size: 1601 46 1230 1286
Fragments arranged by size:
1601 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1177 1955 2145 3005 1177
Size: 778 190 860 2335
Fragments arranged by size:
2335 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 1177 1788 1881 1955 2145 2207 3005 1177
Size: 611 93 74 190 62 798 2335
Fragments arranged by size:
2335 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2595 2595
Size: 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 142 1213 1427 1729 2110 142
Size: 1071 214 302 381 2195
Fragments arranged by size:
2195 1071 381 302 214
BsrBI CCG'CTC
Cuts at: 50 129 2455 2509 3993 4158 50
Size: 79 2326 54 1484 165 55
Fragments arranged by size:
2326 1484 165 79 55 54
BsrDI GCAATG_nn'
Cuts at: 119 2075 119
Size: 1956 2207
BsrFI r'CCGG_y
Cuts at: 2161 2342 2161
Size: 181 3982
BssSI C'ACGA_G
Cuts at: 2434 3142 2434
Size: 708 3455
BstAPI GCAn_nnn'nTGC
Cuts at: 1540 1540
Size: 4163
BstBI TT'CG_AA
Cuts at: 2524 2524
Size: 4163
BstYI r'GATC_y
Cuts at: 1 1188 1655 1678 2013 2259 2565 2577 2663
Size: 1187 467 23 335 246 306 12 86
Cuts at: 2663 3715 1
Size: 1052 449
Fragments arranged by size:
1187 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 515 686 515
Size: 171 3992
BtgI C'CryG_G
Cuts at: 107 155 107
Size: 48 4115
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2113 2294 2457 2113
Size: 181 163 3819
Fragments arranged by size:
3819 181 163
BtsI GCAGTG_nn'
Cuts at: 570 1406 570
Size: 836 3327
DraI TTT'AAA
Cuts at: 843 1162 843
Size: 319 3844
DraIII CAC_nnn'GTG
Cuts at: 918 918
Size: 4163
DrdI GACnn_nn'nnGTC
Cuts at: 902 1869 3213 902
Size: 967 1344 1852
Fragments arranged by size:
1852 1344 967
EaeI y'GGCC_r
Cuts at: 141 1748 1922 2313 2340 3685 141
Size: 1607 174 391 27 1345 619
Fragments arranged by size:
1607 1345 619 391 174 27
EagI C'GGCC_G
Cuts at: 1748 1748
Size: 4163
EarI CTCTTCn'nnn_
Cuts at: 803 1082 2186 2396 803
Size: 279 1104 210 2570
Fragments arranged by size:
2570 1104 279 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2371 3113 3259 3973 2371
Size: 742 146 714 2561
Fragments arranged by size:
2561 742 714 146
EcoICRI GAG'CTC
Cuts at: 1175 1175
Size: 4163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 524 1052 1094 1121 1988 2420 2773 3435
Size: 368 528 42 27 867 432 353 662
Cuts at: 3435 156
Size: 884
Fragments arranged by size:
884 867 662 528 432 368 353 42
27
EcoO109I rG'GnC_Cy
Cuts at: 1302 1302
Size: 4163
EcoRI G'AATT_C
Cuts at: 1116 1167 1116
Size: 51 4112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 835 915 2182 835
Size: 80 1267 2816
Fragments arranged by size:
2816 1267 80
FspI TGC'GCA
Cuts at: 1944 1944
Size: 4163
HaeII r_GCGC'y
Cuts at: 212 1597 1845 3075 3846 212
Size: 1385 248 1230 771 529
Fragments arranged by size:
1385 1230 771 529 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 358 384 390 416 912 944 2005 2035 2037
Size: 26 6 26 496 32 1061 30 2
Cuts at: 2037 2067 358
Size: 30 2454
Fragments arranged by size:
2454 1061 496 32 30 30 26 26
6 2
HincII GTy'rAC
Cuts at: 455 1202 455
Size: 747 3416
HpaI GTT'AAC
Cuts at: 455 455
Size: 4163
KasI G'GCGC_C
Cuts at: 1593 1841 1593
Size: 248 3915
KpnI G_GTAC'C
Cuts at: 1183 1183
Size: 4163
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2922 3106 2922
Size: 184 3979
MscI TGG'CCA
Cuts at: 143 1924 143
Size: 1781 2382
MslI CAynn'nnrTG
Cuts at: 112 284 2152 2279 112
Size: 172 1868 127 1996
Fragments arranged by size:
1996 1868 172 127
MspA1I CmG'CkG
Cuts at: 340 1274 1588 1948 2732 2977 340
Size: 934 314 360 784 245 1526
Fragments arranged by size:
1526 934 784 360 314 245
NaeI GCC'GGC
Cuts at: 2344 2344
Size: 4163
NarI GG'CG_CC
Cuts at: 1594 1842 1594
Size: 248 3915
NcoI C'CATG_G
Cuts at: 107 107
Size: 4163
NgoMIV G'CCGG_C
Cuts at: 2342 2342
Size: 4163
NspI r_CATG'y
Cuts at: 1216 1716 3319 1216
Size: 500 1603 2060
Fragments arranged by size:
2060 1603 500
PciI A'CATG_T
Cuts at: 3315 3315
Size: 4163
PflMI CCAn_nnn'nTGG
Cuts at: 362 1066 362
Size: 704 3459
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2609 2641 2609
Size: 32 4131
PsiI TTA'TAA
Cuts at: 706 851 863 706
Size: 145 12 4006
Fragments arranged by size:
4006 145 12
PstI C_TGCA'G
Cuts at: 345 1210 1895 345
Size: 865 685 2613
Fragments arranged by size:
2613 865 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 340 1588 1948 340
Size: 1248 360 2555
Fragments arranged by size:
2555 1248 360
RsrII CG'GwC_CG
Cuts at: 2358 2358
Size: 4163
SacI G_AGCT'C
Cuts at: 1177 1177
Size: 4163
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 3680 3680
Size: 4163
SfcI C'TryA_G
Cuts at: 33 332 341 1206 1891 2859 3050 33
Size: 299 9 865 685 968 191 1146
Fragments arranged by size:
1146 968 865 685 299 191 9
SfoI GGC'GCC
Cuts at: 1595 1843 1595
Size: 248 3915
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SmaI CCC'GGG
Cuts at: 1185 1185
Size: 4163
SmlI C'TyrA_G
Cuts at: 1318 2233 2947 3209 1318
Size: 915 714 262 2272
Fragments arranged by size:
2272 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 1216 1716 1216
Size: 500 3663
StyI C'CwwG_G
Cuts at: 107 1297 1651 107
Size: 1190 354 2619
Fragments arranged by size:
2619 1190 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1746 1879 2338 2547 1746
Size: 133 459 209 3362
Fragments arranged by size:
3362 459 209 133
TatI w'GTAC_w
Cuts at: 3678 3678
Size: 4163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3680 3712 3680
Size: 32 4131
Tth111I GACn'n_nGTC
Cuts at: 1960 1960
Size: 4163
XbaI T'CTAG_A
Cuts at: 1194 1194
Size: 4163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1658 1658
Size: 4163
XmaI C'CCGG_G
Cuts at: 1183 1183
Size: 4163
XmnI GAAnn'nnTTC
Cuts at: 680 680
Size: 4163
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI
BspEI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI