Restriction map for pF1KB5099
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflIII      AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AvaI        AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BclI        BcnI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstZ17I     
CelII       ClaI        CpoI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
FbaI        FseI        HaeII       Hin1I       HincII      
HindII      HpaI        KasI        KpnI        Ksp632I     
KspI        MaeII       MluI        MluNI       MroI        
MscI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NotI        NruI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccII          45  CGCG                 CG^CG
AocI         2037  CCTGAGG              CC^T[ACGT]AGG
ApaI         2198  GGGCCC               GGGCC^C
AspI          886  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        2047  TGCGCA               TGC^GCA
BanII        2198  GGGCCC               G[GA]GC[CT]^C
BciVI        1384  GGATAC               GTATCC(6/5)
BpmI          437  CTCCAG               CTGGAG(16/14)
BsaBI        1849  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI        1583  GAGGAG               GAGGAG(10/8)
BsrFI         569  ACCGGC               [GA]^CCGG[CT]
BstUI          45  CGCG                 CG^CG
Bsu36I       2037  CCTGAGG              CC^T[ACGT]AGG
CfoI         2048  GCGC                 GCG^C
Cfr10I        569  ACCGGC               [GA]^CCGG[CT]
Eco81I       2037  CCTGAGG              CC^T[ACGT]AGG
EcoRI         414  GAATTC               G^AATTC
EcoRV         625  GATATC               GAT^ATC
EcoT14I       720  CCAAGG               C^C[AT][AT]GG
FspI         2047  TGCGCA               TGC^GCA
HapII         570  CCGG                 C^CGG
HgaI           46  GCGTC                GACGC(5/10)
HhaI         2048  GCGC                 GCG^C
HinP1I       2048  GCGC                 G^CGC
HpaII         570  CCGG                 C^CGG
MamI         1849  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1565  CAATTG               C^AATTG
MspA1I       1811  CAGCTG               C[AC]G^C[GT]G
MspI          570  CCGG                 C^CGG
MunI         1565  CAATTG               C^AATTG
MvnI           45  CGCG                 CG^CG
NheI           33  GCTAGC               G^CTAGC
PflFI         886  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          698  CTGCAG               CTGCA^G
PvuII        1811  CAGCTG               CAG^CTG
SfaNI        1344  GATGC                GCATC(5/9)
SfcI          698  CTGCAG               C^T[GA][CT]AG
StyI          720  CCAAGG               C^C[AT][AT]GG
Tth111I       886  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AflII        1069  CTTAAG               C^TTAAG
             2191  CTTAAG              
AspHI         259  GAGCAC               G[AT]GC[AT]^C
             2127  GAGCAC              
BfrI         1069  CTTAAG               C^TTAAG
             2191  CTTAAG              
BsgI            9  GTGCAG               GTGCAG(16/14)
              366  GTGCAG              
BsiHKAI       259  GAGCAC               G[AT]GC[AT]^C
             2127  GAGCAC              
BsmI          318  GAATGC               GAATGC(1/-1)
             2226  GAATGC              
BstYI         141  AGATCC               [GA]^GATC[CT]
             1854  AGATCC              
DraI         1321  TTTAAA               TTT^AAA
             1545  TTTAAA              
EcoT22I       182  ATGCAT               ATGCA^T
             1777  ATGCAT              
FauI           43  CCCGC                CCCGC(4/6)
             1902  GCGGG               
HindIII       392  AAGCTT               A^AGCTT
              743  AAGCTT              
MflI          141  AGATCC               [GA]^GATC[CT]
             1854  AGATCC              
MslI          424  CACCTTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              763  CATTACTGTG          
NlaIV        2197  GGGGCC               GG[ACGT]^[ACGT]CC
             2198  GGGCCC              
NsiI          182  ATGCAT               ATGCA^T
             1777  ATGCAT              
PpuMI        2041  AGGACCT              [GA]G^G[AT]CC[CT]
             2075  AGGACCT             
SspI         1205  AATATT               AAT^ATT
             1316  AATATT              
TaqI         1756  TCGA                 T^CGA
             1797  TCGA                
TthHB8I      1756  TCGA                 T^CGA
             1797  TCGA                
XhoII         141  AGATCC               [GA]^GATC[CT]
             1854  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AvaII       BbvI        BfaI        
BmyI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspHI       BspMI       BsrDI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MvaI        MwoI        
NdeII       NlaIII      PleI        RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SmlI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XmnI        
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